1.A 96-week comparison of de novo combination therapy with lamivudine and adefovir dipivoxil to optimization monotherapy for chronic hepatitis B
Ying YE ; Xiao CHANG ; Jiabin LI
Chinese Journal of Clinical Infectious Diseases 2012;05(3):131-136
Objective To compare the 96-week efficacy of de novo combination therapy with lamivudine ( LAM ) and adefovir dipivoxil (ADV) to that of optimization monotherapy for chronic hepatitis B (CHB).Methods A total of 155 CHB patients were collected from the First Affiliated Hospital of Anhui Medical University during 2007 and 2009.All patients were randomly assigned to LAM monotherapy group ( n =53 ),ADV monotherapy group ( n =50 ) or LAM with ADV combination group ( n =52 ) according to randomized digital table.The liver and kidney functions,HBV serum markers,and HBV DNA loads were tested every 24 weeks.If patients in LAM or ADV group had poor response or virological breakthrough,they were given optimized therapy with ADV or LAM at week 24,48 or 72.One-way ANOVA (normal distribution and homoscedasticity ) and non-parametric test (non-normal distribution ) were performed to compare measurement data among groups.The impact factors of early virological response were analyzed by binary Logistic regression method.Results At week 24,the complete virological responses in LAM group,ADV group,and LAM + ADV group were 66.0% ( 35/53 ),34.0% ( 17/50 ) and 90.4% ( 47/52 ),respectively (x2 =35.282,P < 0.01 ) ; while,at week 96 the complete virological responses in three groups were96.2% (51/53),86.0% (43/50) and 100.0% (52/52),respectively (x2 =19.115,P>0.05).At week 96,the cumulative recover rates of ALT in LAM group,ADV group,and LAM + ADV group were 86.8% (46/53),82.0% (41/50)and 94.2% (49/52),respectively (x2 =3.613,P >0.05);however,the ALT levels in three groups were statistically different (x2 =11.195,P < 0.01 ).At week 96,the HBeAg seroconversion rates in LAM group,ADV group,and LAM + ADV group were 31.3% ( 10/32),20.7% ( 6/29 ) and 38.7% ( 12/31 ),respectively (x2 =2.313,P > 0.05 ).Early virological response was not found in I patient in LAM group and 19 patients in ADV group; virological breakthrough occurred in 11 patients in LAM group and 1 patient in ADV group.All patients in LAM + ADV group had early virological responses and had no virological breakthrough.Logistic regression showed that complete virological response at week 24 was correlated with the baseline HBeAg,the initial treatment and HBV DNA load.Layered evaluation showed that there were significant differences in early complete virological responses among three groups for patients with positive HBeAg,HBV DNA > 6.28 × 106 copies/mL and ALT ≤5 ×ULN (x2 =7.726,10.921 and6.100,P<0.05 or <0.01) ; for those with HBV DNA >6.28 × 106copies/mL,complete virological response was not observed in ADV group treated for 24 weeks.Conclusion LAM combined with ADV has stronger antiviral activity,lower resistance rate and can improve liver function and virological response,especially for the patients with HBeAg-positive,high HBV DNA loads and ALT ≤5 × ULN.
2.Advance in Brain Mechanism in Nervous and Mental Diseases Based on Resting-state Networks (review)
Juan XIAO ; Jingling CHANG ; Ying GAO
Chinese Journal of Rehabilitation Theory and Practice 2013;19(8):743-747
As a vital part of human body, the brain executes junior and senior function through coordination and interaction of different functional regions. Modern scientific research showed that there were many human brain functional networks in the resting-state. The resting-state functional magnetic resonance imaging technology, which was getting more and more mature, taking opportunities for brain functional networks and was widely used in nervous and mental diseases, providing new methods and ideas for the diagnosis and assessment of nervous and mental diseases. This paper focused on the brain mechanism research methods based on resting-state networks and its application in nervous and mental diseases.
3.The Inhibitory Effect of Recombinant Solube KDR and Its Antibody on Endotheliocyte Proliferation
Li LIU ; Chang XIAO ; Ye WANG ; Ying CAO
Chinese Journal of Cancer Biotherapy 1994;0(01):-
Objective: To investigate the inhibitory effect of soluble KDR(sKDR) and its antibody on endotheliocyte(EC) proliferation.Methods: The binding of sKDR with VEGF was detected by ELISA. The KDR262 antiserum was prepared from rabbit and the specificity was defined by Western blot. The inhibition of EC proliferation was analyzed by 3H-TdR up-take, MTT and cell counter. Results: The sKDR could bind to VEGF165 specially, the ability was enhanced by heparin. The specific binding of KDR262 antiserum to KDR was also detected. The inhibitory rate of sKDR(10,2,0 4?g/ml) on EC proliferation was 56%,44%,32% respectively. The inhibitory rate of KDR262 antiserum (1∶50,1∶200,1∶800) was 70%, 56%,43% respectively. The cell counter also showed that EC proliferation was inhibited significantly in sKDR group and KDR262 antibody group, vs VEGF165 group, GST group and PBS group. The inhibitory effect was characterized by concentration-dependent and was higher in KDR262 antibody group than that in sKDR group. Conclusion: sKDR expressed by E.coli and its antibody had significant inhibitory influence on EC proliferation.
4.STUDDZS OF SCREENING MICROORGANISMS DEGRADING LINEAR SODIUM ALKYLBENZENESULFONATE (LAS) AND ITS DEGRADINGCHARACTERISTICS
Qi-Feng YING ; Chang-Song XIAO ; Shu-Lan JI ;
Microbiology 1992;0(01):-
A bacterium which can effectively degrade LAS (Linear Sodium Alkylbenzenesulfonate) was isolated from washing powder manufacturing effluent and was preliminarily identified as Corynebacterium jeikeium GZ6. The bacterium can degrade LAS up to till 700 mg/L, and the optimum pH, temperature and concentration of LAS are 7.0, 30℃ and 400 mg/L, respectively. The biodegradation rate can reach 98.7% after 24 hours'cultivation in the suitable conditions. Experiments also showed that some heavy metal ions such as Hg2+ , Co2+ , Cd2+ can differently inhibit the degradation of LAS.
5.Sequence structure and phylogenetic analysis of the chloroplast genomes of Alangium chinense (Lour.) Harms and its different subspecies
Xiao-ying YANG ; Chang LIU ; Xian-fa ZENG ; Xiong-wei LIU ; Jie-hong ZHAO ; Ting-ting FENG ; Ying ZHOU
Acta Pharmaceutica Sinica 2022;57(10):3229-3239
italic>Alangium chinense is a commonly used medicinal plant of Alangiaceae
6.Adverse drug reactions induced by Mailuoning injection: literature review on 162 cases.
Gui-Ying XING ; Chang-Yu LI ; Yuan-Xiao YANG
China Journal of Chinese Materia Medica 2008;33(11):1322-1326
To understand the adverse drug reaction (ADR) induced by Mailuoning injection. 162 ADRs due to the drug were retrieved from national medical journals of 1988-2007 for statistics. It was shown that there was no relationship between ADR and dosage, but ADR appeared mostly in middle-aged and old groups, and more in male than in female. The occurrence of ADR was commonly within 30 min after injection. It involved injuries of various systems and organs. As for the 123 cases of allergy, 38 were anaphylactic shock (accounting for 23.46%), two people died. ADR characterized by immediate type on initial use and tachy type. It should be paid more attention to the Mailuoning injection for the immediate hypersensitivity reaction (such as anaphylactic shock).
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7.Synergic effects of bexarotene and TRAIL on apoptosis of leukemic cell line KG1a
Liyu ZHOU ; Zheng ZHANG ; Shaoxu YING ; Xiao LI ; Chunkang CHANG ; Qi HE ; Quan PU
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(11):1316-1319
Objective To explore the effects and mechanism of bexarotene in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on apoptosis of leukemic cell line KG1a. Methods KG1a cells at logarithmic growth phase were obtained, and were divided into TRAIL group, bexarotene group, 300 ng/mL TRAIL in combination with bexarotene group and 2.0 μmol/L bexaroten in combination with TRAIL group. Cell apoptosis rate was detected in each group by flow cytometry. Flow cytometry was also employed to determine the apoptosis rates of KG1a cells after treatment with bexarotene and TRAIL in different sequences. The expression of Fas associated death domain-like IL-1 beta converting enzyme inhibitory protein (c-FLIP) was detected by Western blotting. Results There was no significant difference in cell apoptosis rates between TRAIL group and bexarotene group of each concentration (except for bexarotene 2.0 μmol/L) (P > 0.05). The cell apoptosis rates of 300 ng/mL TRAIL in combination with bexarotene group and 2.0 μmol/L bexaroten in combination with TRAIL group were significantly higher than those in TRAIL group and bexarotene group of each corresponding concentration (P <0.01). Sequential analysis revealed that bexarotene could reverse the resistance of KG1a cells to TRAIL (P < 0.001). Compared with single use of 2.0 μmol/L bexarotene or 300 ng/mL TRAIL, combination use could significantly down-regulated the expression of c-FLIP (P < 0.05). Conclusion Bexarotene can significantly enhance the apoptosis of KG1a cells induced by TRAIL, which may be attributed to the down-regulation of c-FLIP expression.
9.Echinacoside and Cistanche tubulosa(Schenk)R.wight have male reproductiveprotection effect by targeting hypothalamic and rogen receptor
Xiao-Ying ZHANG ; Zhi-Hui JIANG ; Xue-Mei CHANG ; Rui-Min CAO
Chinese Journal of Pharmacology and Toxicology 2018;32(4):257-258
OBJECTIVE Cistanche deserticola Y. C. Ma is an official plant that grows in arid or semi-arid areas.Our early work demonstrated that Cistanche extracts protect against sperm damage in mice under bisphenol A induced reproductive damage. Echinacoside (ECH)is one of the major active components. The present study investigated the mechanisms behind the possible protective effects of ECH against oligoasthenospermia in rat and identified the interaction between ECH and AR. METH-ODS The distribution of ECH was assayed by HPLC,the quantity and quality of sperm was evaluated and hormone levels were determined by radioimmunosorbent assay. The levels of androgen receptor (AR)and key steroidogenic-related genes were reduced as determined by Western blotting and qPCR analysis.The interaction between ECH and AR were evaluated by fluorescence localization assay,indi-rect ELISA and molecular docking. RESULTS ECH significantly increased the quantity of sperm and secretions of luteinizing hormone and testosterone.ECH was distributed to the hypothalamus but not in the testes.ECH combined with hypothalamic AR in the pocket of Met-894 and Val-713 to inhibit transfer of AR from the cytoplasm to nuclei in the hypothalamus.While negative feedback of sex hormone regula-tion was inhibited, positive feedback was stimulated to increase the secretion of luteinizing hormone and testosterone subsequently enhancing the quantity of sperm. C. militaris significantly alleviated the BPA-induced reproductive damage by increasing testicular superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and glutathione (GSH); as well as by reducing serum malondialdehyde (MDA). C. militaris not only obviously enhanced the levels of serum LH and T, but it also improved the sperm count and motility compared to the BPA-treated group.CONCLUSION C.militaris and ECH protect the BPA induced reproductive damage.ECH blocks AR activity in the hypothalamus to increase the quantity of sperm and protect against oligoasthenospermia in rats.
10.Effect of synapsin-Ⅰ antisense oligonucleotide on neural differentiation of embryonic stem cells in vitro
Yan-Ling LIANG ; Su-Ming ZHANG ; Min ZHANG ; Li-Ying CHANG ; Xiao-Qing LI ;
Chinese Journal of Neurology 1999;0(06):-
Objective To investigate the role of synapsin-I in the differentiation of the embryonic stem cells (ESC) into the neuron,and to seek a controllable point for the ESC neural differentiation in vitro.Methods Neural differentiation of ESC was induced with the "five step approach",synapsin-I antisense oligonucleotides (AS ONs) was employed to inhibit the synapsin-I expression at different stages. At different time points,morphology,differentiation efficiency and neural specific markers were compared among the normal group and the transfected groups.A tumor cell line called PC12 cell was compared with the ESC at the same time.Results After the synapsin-I AS ONs were used in ESC differentiation, considerable decreases of neurite growth rate and neural precursor cells (nestin (+)) percentage were observed at Stage 3(68.5%?4.2% vs 76.2%?5.1% and 75.8%?4.9%,P