Objective To determine the status of self-efficacy and self-care agency of the empty nest elderly patients in the community,and analyze their correlation. Methods A total of 320 empty nest elderly with chronic disease in Fushun, Liaoning Province. The General Information Questionnaire, Exercise of Self-Care Agency scale (ESCA) and the Chronic Disease Self-efficacy Scale were used to investigate the empty nest elderly with chronic disease in the community and analyze the present situation level of the self-care agency and the self-efficacy and the correlation between them. Results General data showed no significant difference between solitary empty-nest elderly and connubial empty-nest elderly (P>0.05). Solitary empty-nest elderly got (93.36 ± 13.49) points and (5.22 ± 0.62) points in ESCA and the Chronic Disease Self-efficacy Scale,which were lower than (98.10±11.34) points and (5.32±0.30) points of connubial empty-nest elderly, the difference was statistically significant (t=-0.866,-0.467, P<0.05). There was significantly positive correlation between self-efficacy and self-care agency (r=0.318, P<0.01). Conclusions There is positive correlation between self-efficacy and self-care agency. It is suggested to implement community-based nursing interventions to enhance their self-efficacy and to promote their self-care agency,there by to improve their quality of life .

Arthritis, Rheumatoid ; diagnosis ; drug therapy ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods

Objective　To study the alterations of surfactant protein A (SP-A) and B (SP-B) in rats in the early stage of acute lung injury induced by oleic acid. Methods　An acute lung injury model was established by injecting oleic acid into the superior vena cava of rats and the levels of SP-A and SP-B in branchoalveolar lavage fluid (BALF) were determined with Western Blot and ELISA. Results　The levels of SP-A and SP-B in BALF were significantly decreased in the injured group 1 h after acute lung injury (P<0.01) and remained low in the next 6 h. Conclusion　The levels of SP-A and SP-B were decreased in the early stage of acute lung injury. The methods used for detecting SP-A and SP-B we adopted are specific and sensitive, and might be helpful for other experimental study.

Establishment of bioassay methods is the technical issues to be faced with in the bioassay of Chinese materia medica. Taking the bioassay of Coptis chinensis Franch. as an example, the establishment process and application of the bioassay methods (including bio-potency and bio-activity fingerprint) were explained from the aspects of methodology, principle of selection, experimental design, method confirmation and data analysis. The common technologies were extracted and formed with the above aspects, so as to provide technical support for constructing pattern and method of the quality control for Chinese materia medica based on the dao-di herbs and bioassay.

Epilepsy, Absence ; Humans

Objective There are two main functions of gastrointestinal tract,digestion and absorption,and barrier function.The latter has an important defensive effect,which keeps the body away from the invading and damaging of bacteria and endotoxin.It maintains the systemic homeostasis.Intestinal dysfunction would happen when body suffers from diseases or harmful stimulations.The more serious intestinal disorders would harm the intestinal protective mechanism,or intestinal barrier function,and bacterial/endotoxin translocation,of intestinal failure(IF) would ensue.This article provides a critical review of the evidence indicating that an increase in bacterial translocation is associated with sepsis,and even the multiple organ failure syndrome in critically ill patients.The in-transit microorganisms play an essential role in the homeostasis of local and systemic immunity.Methods All studies published from 2000 to June 2005 about intestinal permeability,bacterial translocation,and systemic inflammatory response syndrome were located by search of PubMed.Results Clinical and experimental studies investigating the correlation between bacterial translocation and systemic inflammatory response syndrome,associated with the damage of the gut barrier function.To keep the mucosal barrier function intact is one of the main issues in the prevention of bacterial translocation.This could be achieved by the adequate delivery of oxygen and nutrient supplementation to the gut.Enteral nutrition,probiotic can be a good choice.Conclusion With a better understanding of the bacteria-host interactions in health and the alterations induced by critical illness,new therapies that improve the environment of both may lead to better recovery rates in intensive care unit patients.

Objective To investigate and analyze the diagnostic significance of humoral and cellular immune function detection in the patients with syphilis.Methods Eighty-six cases of syphilis without anti-syphilis treatment in our hospital were selected as the research subjects(syphilis),including 26 cases of primary syphilis,39 cases of secondary syphilis 21 cases of latent syphilis,and 30 healthy subjects were selected as the control group.Then the humoral immunity and cellular immunity levels were compared between the two groups.Results The levels of IgG,IgA and IgM in the syphilis group were significantly increased compared with the control group(P<0.05).The IgG,IgA and IgM levels had no statistical difference between the primary syphilis patients and control group(P>0.05),which in the patients with secondary syphilis and latent syphilis were significantly increased compared with the control group,moreover the increase in the secondary syphilis was most significant(P<0.05);the CD3+,CD4+,CD4+/CD8+ and NK levels in different stages of syphilis were significantly decreased compared with the control group(P<0.05),while the CD8+ level was significantly higher than that in the control group(P<0.05),in which the change in the secondary syphilis patients was most significant.Conclusion The humoral immune in the patients with syphilis is hyperfunction,while cellular immune is dysfunction,the patients present the overall immune function disorder,therefore the humoral and cellular immunity detection has a certain clinical value in the diagnosis of syphilis.

Objective To study the effect and mechanism of gene PBP2 and gene PBP3 in drug resistance of Klebsiella pneumoniae(KP) to different antibacterial agents.Methods A total of 203 strains of KP which isolated from clinical samples in each department in our hospital during the period from April 2014 to April 2015.Strains were identified and antibiotic susceptibility were determined with automatic bacteria identification system,then extracted DNA genome from bacteria,proceeded with electro-phoresis after the product of PBP2 and PBP3 were amplified by PCR,examined genetic expression with RT-PCR,and study their connection to drug-fast of the KP combining results of antibiotic susceptibility.Results In 203 strains of KP,105 strains resistant to β-lactam drug (51.7%),88 strains were sensitive,resistant rate to imipenem and meropenem were 5.4%,3.9% respectively.The PCR amplification products of the electrophoresis bands of PBP2,PBP3 gene,18 strains were negative,185 strains were positive,which was not parallel,there were respectively negative and positive strains among them.There was no significant correlation between PBP2,PBP3 and multiple drug resistance of KP(P=0.295,0.628).PBP2 was not related to the resistance of carbapenem(P=0.637).PBP3 had correlation with carbapenem resistance,but the correlation coefficient was not high(P=0.041,r=0.148).After reverse transcription of PBP2,PBP3,statistically analyze electrophoresis bands of amplification products of carbapenem-resistant KP and 16SrRNA amplification bands of gray gradient ratio,there was no statistical significance(PBP2:P=0.331,PBP3:P=0.383).Conclusion Gene PBP2,PBP3 might not be the necessary reasons of KP being multiple drugs resistant.

After the need of medical information professionals was analyzed, the reform of training model for medi-cal information professionals in Department of Information Management and Information System, Hubei Medical and Pharmaceutical College, was described in aspects of the position of its training target, construction of its teaching team, optimization of its course teaching system structure, updating of its teaching methods, and improvement of its practical ability training system in order to provide certain beneficial experiences and reference for the training of medical information professionals in higher education institutions.

Objective To investigate the association between systemic lupus erythematosus(SLE) and polymorphism of cytotoxic T-lymphocyte antigen 4 gene(CTLA-4) and the effect on ANA and anti-dsDNA antibody.Methods The A/G substitution was investigated in the exon 1 position 49 in 92 SLE patients and 60 controls by PCR restriction fragment length polymorphism(PCR-RFLP).ANA and anti-dsDNA antibody were examined by indirect fluorescence immunoassay.Results SLE patients had significantly higher frequencies of the CTLA-4+49GG allele than controls(P