Principles of constructing medical information technology curriculum system were pro-posed revolving round the core problem of how to combine needs of developing life science with culturing talents who can independently apply information technology knowledge in studying and researching. De-tailed implementing measures for constructing medical information technology curriculum system were summarized after repeated practical teaching. Longitudinal classification based on discipline and trans-verse classification based on teaching level were combined to construct the whole standard course. Finally, courses were appropriately distributed according to detailed culturing objectives for students in medical universities and colleges,thus a suitable medical information technology curriculum system in medical universities and colleges was formed.

After the need of medical information professionals was analyzed, the reform of training model for medi-cal information professionals in Department of Information Management and Information System, Hubei Medical and Pharmaceutical College, was described in aspects of the position of its training target, construction of its teaching team, optimization of its course teaching system structure, updating of its teaching methods, and improvement of its practical ability training system in order to provide certain beneficial experiences and reference for the training of medical information professionals in higher education institutions.

Objective To approach the clinical characteristics of extrapulmonary complication induced by mycoplasma pneumoniae(MP) infection and infectious mononucleosis syndrome. Methods The MP-IgM(+), EBV- IgM(+) were determined by enzyme- linked immunosorbent assay.There were 18 cases infected with MP and infectous mononucleosis syndrome with abnormal fever,cervix lyphadenectasis,pharyngitis,liver and spleen intumescence,lung infection, and so on.They were analyzed by reviewing.Results There were 8 cases(44.4%) with extrapulmonary complication, among them 3 cases(16.6%) with liver injury,1 case (5.56%) with heart injury,2 cases(11.1%) with both heart and liver injuries,1 case(5.56%) with urinary system injury (nephritis), 1 case (5.56%) with nervous system injury (encephalitis).The complication usually arised 1 week after primary affection, but had no typical clinical symptoms.There was only 1 case with encephalitis who expressed the symptom of nervous system symptom firstly, and was clinically dia- gnosed by assistant examinations. All the cases recovered after 3 weeks′ treatment with macrolides, 5-7 days with ganciclovir, and also with regular complication and support treatments.Conclusions The incidence of extrapulmonary complication induced by MP infection and infectous mononucleosis syndrome is higher than that by the single infection, and its clinical symptom isn′t obvious and is easy to be covered by original illness. And a few showing the extrapulmonary as the first symptom is easily mistakenly diagnosed .As a result, the prognosis is favorable if we make subsidiary inspection and diagnosis as soon as possible and give it with regular treatment.

The diagnosis of myocardial damage in systemic lupus erythematosus(SLE) is mainly based on clinical symptoms,physical examinations and electrocardiographic abnormality,echocardiographic changes,and myocardial enzymologic diversity in general.In recent years,it has been suggested that cardiac troponin I possesses high sensitivity and specificity in the diagnosis of myocardial damage.Lupus myocardial damage is diagnosed definitively depending on endomyocardial biopsy.Its treatment has been empirical,and hormone combined with immunodepressive is routine treatment.According to pathogenetic condition,there are still several therapies such as intravenous immunoglobulin therapy,blood purification and autologous hematopoietic stem cell transplantation,extracorporeal membrane oxygenation,and so on.Most often,myocardial dysfunction in SLE is of subclinical progression,and that early diagnosis and prompt treatment may play an important role in relieving patients' condition,raising quality of life and improving prognosis.

Reflex sympathetic dystrophy after stroke,also called shoulder-hand syndrome(SHS),is a common complication of stroke.It is presumed that SHS is consequence of many factors,including periphery injury,effect of sympathetic never system,neurogenic inflammation,and plasticity of central nervous system.

Microbes are important composition of ecology system. Nowadays it's very important to study the microbial diversity and community structure, especially in water bioremediation by microbiological resources. Modern molecular biology techniques provide effective methods to study the microbial ecosystem in aqua. Several techniques were summarized, including 16S rDNA clone library, DGGE (Denaturing gradient gel electrophoresis), RFLP (Restriction Fragment Length Polymorphism) and T-RFLP (Terminal Restriction Fragment Length Polymorphism), while their application to analyze water microbial diversity were introduced.

Lipases have catalytic active in both aqueous phase and the non-aqueous phase and have a wide range of application in various industrial areas.However,the high cost of lipase production has restricted its extensive use in industry.Solid state fermentation possesses many advantages,such as low requirement for devices,low energy consumption,low production cost,little pollution to environment and easily being popularized,which have made it an important means in microbial production of lipases.Owing to the rapidly increased energy cost and the people's awareness of environmental protection,the solid state fermentation technique,which was regarded as low-tech in the past,has regained attention and developed rapidly since the 1990s.The production of lipase by SSF technique was reviewed.Mainly contents describe its characteristics,including physical and chemical factors and bioreactors.

Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on regulatory T cells(CD4+CD25+ Foxp3+ T cell)and airway inflammation in asthmatic rats. Methods Asthmatic models were established in 28 rats with ovalbumin as sensitinogen,and four groups were randomly divided(n=7).1,25(OH)2D3 was orally administered 1 h before challenge in treatment group 1,dexamethasone was subcutaneously injected 1 h before challenge in treatment group 2,both 1,25(OH)2D3 and dexamethasone were given 1 h before challenge in treatment group 3,while no medicine was administered 1 h before challenge in group 4(asthmatic control group).The levels of IL-4 and IL-10 in bronchoalveolar lavage fluid(BALF) were detected,the total cell numbers were obtained and the eosinophil count was performed.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells were measured with flow cytometry,and the mRNA expression of IL-10 and Foxp3 in lung tissues was detected.Wistar rats without asthma were served as normal controls(n=7). Results There were significant differences between asthmatic control group and normal control group in each parameter.Compared with asthmatic control group,the total cell number and eosinophil count decreased,the level of IL-4 decreased and the level of IL-10 increased in BALF in treatment groups.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells significantly increased,and the mRNA expression of IL-10 and Foxp3 in lung tissues increased in treatment groups compared with asthmatic control group.There was no significant difference between treatment groups and normal control group in each parameter. Conclusion Administration of 1,25(OH)2D3 increases the proportion of Treg and expression of Foxp3 mRNA in peripheral blood and spleen in asthmatic rats.1,25(OH)2D3 has a regulatory effect on Treg,and may serve as a potential therapeutic approach against asthma.

Stereotactic radiotherapy is a rapidly growing treatment strategy for tumor due to the advances in target definition,radiation dose delivery,and image guidance of radiation.We reviewed the clinical application of stereotactic radiotherapy in the management of early stage non-small cell lung cancer including the technique,the workflow of the technique established,the outcome and relative radiation toxicity.