Endophthalmitis ; complications ; drug therapy ; microbiology ; Humans ; Infant, Newborn ; Meningoencephalitis ; complications ; drug therapy ; microbiology ; Suppuration ; complications

Objective:To explore clinical and histopathological characteristics of primary biliary cirrho-sis-autoimmune hepatitis overlap syndrome.Methods:Clinical data and pathological findings of 10 pa-tients were reviewed.Results:Serum glutamine transpeptidase,alkaline phosphatase levels,alaninetransaminase,aspartate transaminase,serum IgG and IgM were elevated in all the patients.They were allpositive for anti-mitochondrial antibody and AMA-M2.Nine patients were positive for anti-nuclear anti-body and one patient was positive for anti liver-kidney microsome antibody.Liver biopsies in these pa-tients revealed:ten patients had bile duct lesion,hepatitis activities ranged from moderate to severe,andfibrosis ranged from S1 to S3.Conclusion:PBC-AIH overlap syndrome is mostly found in middle-agedwomen.It has the clinical and histopathological characteristics of both PBC and AIH.Accurate andprompt diagnosis of overlap syndrome patients should be based on the clinical presentation,biochemicaland immune indexes,and hepalic pathological changes.

Objective To analyze ultrasonic manifestations of intrahepatic and perihepatic abnormalities after liver transplantation.Methods Ultrasonograms of intrahepatic and perihepatic abnormalities in 114 patients after liver transplantation were retrospectively analyzed.Results Among 114 patients,perihepatic effusion was observed in 96,perihepatic hematoceles or hematoma formation in 19,intrahepatic hematoma in 1,ischemic necrosis of hepatic segments in 2,bile leakage in 7,biloma in 3,focal fatty infiltration of transplanted liver in 3 and intrahepatic solid masses in 6 patients.Conclusion Ultrasonography has great application value in diagnosis of intrahepatic and perihepatic abnormalities after liver transplantation.

Objective To explore the development on infrastructure and professional contingence related to injury prevention and control, in China. Methods 38 Centers for Disease Control and Prevention(CDC)were investigated by using a self designed questionnaire and data was analyzed descriptively. Results At present, all the CDCs at provincial and city level had not set up a specific department related to injury prevention and control, except for Shanghai and Guangxi CDCs.The proportions of full-time and part-time staff in all of the investigated CDCs were 37.30% and 62.70% respectively. From 2005 to 2008, the proportions of CDC which had made funds more than 500 000 Yuan available on injury prevention and control were: 27.78% in the eastern areas, 28.58% in central and 7.69% in the western areas of China. There were 76.92% of the CDCs in the western areas of China that the invested funding was less than 100 000 Yuan in the past years. Most of the routine work that had been carried out in those CDCs were surveillance and public education programs including collection of data and special surveys related to injuries on children, adolescents and the elderly population. 44.44%, and 28.57% of the CDCs in the eastern and central parts of the country wished to establish a Department of injury prevention and control, while 76.92% of the CDCs in the western part expressed their strong request for professional training on injury. Conclusion China remained underdeveloped in the development of institutional and professional team working on injuries which called for, setting up related programs to suit the local needs. In accordance with the working condition, the progress that had been made and the objective demand on institutional and professional contingence of the problems in different areas,both short and medium terms on the issue,need to be put forward to develop both institutional and professional programs on injuries in the eastern, central, and western areas of China.

Objective To find a way to measure and count plane distribution of cells distributed on single layer and compare differences of undifferentiated mesenchymal cells of periosteum germinal layer from different parts of the body.Methods After counting the number of undifferentiated mesenchymal cells of periosteum germinal layer from different parts of the body microscopically and figuring out the number of cells per area unit in each periosteum specimen,the obtained data were statistically analyzed and the stratum structure of periosteum observed microscopically.Results The homogeneity of variance test showed homoscedasticity,with no statistical significance(P＞0.05).The analysis of variance found homoscedasticity but showed no statistical significance(F=0.253,P＞0.05).The periosteum of patel- la,tibial plateau and costa had two layers,while the periosteum of costal cartilage had three layers. Conclusions There is no conspicuous difference upon proliferation and evoluting activities of periosteum from different parts of body.Therefore,it is unnecessary to choose specific parts for drawing the periote- um in clinical situation.In the meantime,the structure of periosteum from different parts diversifies.

Objective To study the incidence and risk factors of post-operative pain after receive offpump coronary artery bypass grafting(OPCAB) and totally endoscopic coronary artery bypass graft on beating heart(BH-TECAB).Methods Totals of 80 patients received coronary artery bypass grafting were divided into two groups according to the different of operation procedure,BH-TECAB group(40 cases)and OPCAB group(40 cases).Basic situation,anesthesia conditions,surgical conditions,postoperative situation,and the incidence and degree of postoperative pain in two groups were collected.Results There was no significant difference between two groups in the basic situation.All patients had no postoperative complications and recovered smoothly.The incidence of 1-degree,2-degree and 3-degree postoperative pain in the BH-TECAB group [ 32.5％ ( 13/40),7.5％ (3/40) and 2.5％ ( 1/40 ) ] was significantly lower than that in the OPCAB group [45.0％ ( 18/40),15.0％ (6/40) and 10.0％ (4/40) ],the difference was significant (P ＜0.05).The time of breathing machine using,blood transfusion in 24 hours,the amount of flow in BH-TECAB group,respectively,was ( 14.35 ± 1.66 ) h,( 54.79 ± 15.69 ) ml,( 227.12 ± 120.38 ) ml,that significantly lower than that in OPCAB group which was ( 18.64 ± 1.57) h,( 120.75 ± 20.98 ) ml and ( 389.17 ± 138.66 ) ml,the difference was significant ( P ＜ 0.05 ).Conclusions The different of the operation procedure influences the incidence of postoperative pain of patients receive coronary artery bypass grafting.BH-TECAB has the features of mini-wound,less pain and fast healing.

Objective The present study was conducted to identify the Vibrio cholera type and to analyze its antibiotic resistance in an epidemic of cholera in Haiyan County in 2018, which would provide the references for prevention and control of cholera. Methods Stool samples of the patient and his close contacts as well as the food and environmental samples were collected for identification of the type of Vibrio cholerae and the toxin gene. The resistance of identified Vibrio cholerae to 20 different common antibiotics were tested. Results A total of 176 samples were collected, including 101 stool samples from the case and his close contacts, 35 environmental samples and 40 food samples. Among those samples, only one strain of V. cholerae, O139, was isolated from the patient's first feces sample. It was detected as a toxin gene of ctxA positive by real-time fluorescence PCR. Antibiotic resistance test showed that the strain was sensitive to norfloxacin, levofloxacin, ciprofloxacin, cefotaxime, cephalothin, ampicillin, and amoxicillin. It was 100% resistant to tetracycline, doxycycline, neomycin, kanamycin, streptomycin, and rifampicin. Conclusion V. cholerae O139 strain with ctxA is detected in an epidemic of cholera. Norfloxacin, levofluoxacin and some other antibiotics could be used for clinical treatment and prevention. It should pay attention to this strain of V. cholera regarding the multiple drug resistance and the change of antibiotic resistance.

OBJECTIVETo study the epidemiological and clinical characteristics and risk factors of cirrhosis-related hepatocellular carcinomas (HCC) in patients with hepatitis C virus (HCV) infection.

METHODSEighty-nine compensated and decompensated HCV cirrhosis patients were analyzed and followed-up. The main clinical and laboratory variables were analyzed as incidence factors of HCC with univariate analysis and multivariate analysis regression models.

RESULTSThe patients were followed-up for 86 months. Thirty-five of the 89 patients had HCC during the 86 months follow-up. Their five and ten-year cumulative incidences were 16.9% and 40.4% respectively. Of the 35 HCC patients, 4 had a family history of hepatitis C, 12 had a familial history of HCC, and 7 had a history of alcohol ingestion. Five and ten-year cumulative incidences of HCC in patients with hepatic steatosis were 24.6% and 51.0% respectively. Five-year and ten-year cumulative incidences of HCC in patients with non-hepatic steatosis were 8.7% and 26.2% respectively, and the difference in the cumulative incidences between them was significant (P < 0.05). Hepatic steatosis severity was associated with the severity of the cirrhosis. ALT and TBil levels were higher in the HCC group than in the non-HCC group, ALB was lower in the HCC group than in the non-HCC group, and the differences between them were significant (P < 0.05). Child-Pugh score and the severity of the hepatic steatosis during follow-up were independently correlated with HCC.

CONCLUSIONHCC is the most important and frequent outcome of chronic hepatitis C cirrhosis. Child-Pugh score and the severity of the hepatic steatosis are related to the risk factors. History of alcohol ingestion and family history of hepatitis C are also related to liver cancer.

Aged ; Carcinoma, Hepatocellular ; etiology ; Female ; Follow-Up Studies ; Hepatitis C, Chronic ; complications ; Humans ; Liver Cirrhosis ; complications ; Liver Neoplasms ; etiology ; Male ; Middle Aged ; Risk Factors

Adult ; Aged ; Female ; Hepatitis, Chronic ; mortality ; therapy ; Humans ; Liver Failure ; therapy ; Male ; Middle Aged ; Prognosis ; Sorption Detoxification ; Survival Rate

Low levels of coagulation factor VIII (fVIII) protein expression caused by its inefficient secretion and the over-sized fVIII gene affect the transgene-based gene therapy for hemophilia A adversely. Our previous study demonstrated that intein-mediated protein trans-splicing for delivery of the fVIII gene with a dual-vector system could improve secretion of post-translationally spliced fVIII by light chain in cis. In this study, a human/porcine hybrid fVIII (HP-fVIII) containing replaced A1 and A3 domains of porcine fVIII was investigated for secretion and activity of the spliced HP-fVIII after intein-based dual-vector delivery of the HP-fVIII gene. A pair of expression plasmids comprising intein-fused HP-fVIII heavy and light chains were constructed and transiently co-transfected into COS-7 cells. The spliced HP-fVIII and bio-activity in culture media were quantitatively analyzed by ELISA and Coatest method respectively. The intracellular splicing of HP-fVIII was detected by Western blotting. The results showed that in the culture supernatant of cells co-transfected with HP-fVIII, the amount and activity of spliced HP-fVIII were significantly higher than those of spliced hfVIII secreted from the cells co-transfected with human fVIII [(184+/-34 ng/mL) vs (48+/-12) ng/mL, P<0.01; (1.18+/-0.22) IU/mL vs (0.31+/-0.10) IU/mL, P<0.01], demonstrating the dramatically enhancing effect of porcine A1 and A3 domains on the secretion of intein-spliced HP-fVIII. The spliced HP-fVIII protein and its activity were also detected in the supernatant from combined cells separately transfected with intein-fused HP-fVIII heavy and light chain genes, indicating that the intein-mediated HP-fVIII splicing was independent of cellular mechanism and could occur outside the cell after the secretion of precursor proteins. Additionally, an intracellularly spliced HP-fVIII band was found with a molecular weight similar to human fVIII protein, confirming the HP-fVIII splicing. These results provided experimental basis for ongoing study using intein-based dual adeno-associated virus (AAV) vector to transfer HP-fVIII gene in animal models.
Animals ; COS Cells ; Cercopithecus aethiops ; Dependovirus ; genetics ; metabolism ; Factor VIIIa ; biosynthesis ; genetics ; Genetic Vectors ; Humans ; Inteins ; Protein Splicing ; Recombinant Fusion Proteins ; genetics ; Swine ; Trans-Splicing