Graft vs Host Disease ; diagnosis ; therapy ; Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Postoperative Complications ; diagnosis ; therapy ; Transplantation Conditioning

China ; Humans ; Multiple Myeloma ; therapy

Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; therapy

China ; Hematopoietic Stem Cell Transplantation ; Humans

BACKGROUND:During autologous tendon grafting, the ultimate tensile strength used for suturing the end of the ligament is important for successful surgery. Improving suturing strength and increasing the number of stitches is a good choice for increasing the fixed intensity. But excess amount of stitches can produce too many thread residues, thereby affecting tendon healing.
OBJECTIVE:To investigate the essential number of suturing pins for the anterior cruciate ligament revascularization in ligament end suture fixation to reduce suturing thread exposure.
METHODS:(1) In vitro biomechanics test:12 patel ar ligament specimens were divided into two groups:the specimens were sutured with 5 or 3 stitches using Krackow suture method. The suturing thread was J&J tendon suture thread. The strength of tensile was compared between the two groups by Tensile mechanical test was conducted to compare the strength of tensile between the two groups and to explore the optimal number of stitches and suturing method. (2) Clinical application:According to the results of in vitro experiments, modified Krackow suture method was used clinical y for arthroscopic anterior cruciate ligament reconstruction in 125 cases, including 62 cases receiving 3-stitch suture, and 63 cases undergoing 5-stitch suture.
RESULTS AND CONCLUSION:The fixed strength of tensile at suture sites was over 100 N for 3-stitch double-lock suture and 110 N for 5-stitch double-lock suture. There was no significant difference in the fixed strength between the two groups, but their strength values were both over the breaking strength of suturing materials. Moreover, the fixed strength could not be reduced by suturing throughout the ligament at the first stitch. Al the 125 cases were fol owed for 6.4 months averagely, and both 3-stitch and 5-stitch suture methods achieved good outcomes. The satisfaction rate was up to 99%, and no suture breakage or loosing occurred at early and late stages. These findings suggest that, using 3-stitch double-lock suture method, a satisfactory fixed strength can be achieved with reduced thread exposure. The suturing thread can run through the ligament at the first stitch, which can reduce thread exposure but not reduce the fixed strength.

BACKGROUND:The traditional treatment methods for osteoporosis accompanied by bone defects, such as autogenous bone graft, al ograft, biomaterial implants, have significant limitations. The regenerative medicine approach using adipose-derived stem cells as seed cells offers a new way for the repair of bone defects fol owing osteoporosis. OBJECTIVE:To review the pathogenesis of osteoporosis and its impacts on the repair of bone defects, the signal pathway regulation of osteogenic differentiation of adipose-derived stem cells, and the feasibility of adipose-derived stem cells for repairing osteoporotic bone defects. METHODS:A computer-based online search of CNKI database and PubMed database was performed to retrieve the relevant articles published from January 1998 to September 2014 with the key words of“adipose-derived stem cells, osteoporosis, bone defect, osteogenic differentiation, bone regeneration”in Chinese and English, respectively. Final y 77 articles were included for review after deleting unrelated and repetitive ones. RESULTS AND CONCLUSION:In recent years, adipose-derived stem cells have been widely used in regenerative medicine research. With the development of relevant disciplines such as regenerative medicine, tissue engineering, molecular biology, and material science, in-depth studies on regulatory mechanisms of osteogenic differentiation of adipose-derived stem cells have been developed. Adipose-derived stem cells combined with biological scaffolds for construction of tissue-engineered bones provides a new way to repair osteoporotic bone defects.

The standard non-surgery approach for locally advanced non-small cell lung cancer (NSCLC) is concurrent chemoradiotherapy.Common chemotherapy regimens include EP,NP,DC and PC.New chemotherapy drugs combination and targeted therapy are still under study.The tolerances of elderly patients are declining for concurrent chemoradiotherapy,but it is not the absolute contraindication.The tolerance for hyperfraction and high dose radiotherapy is well,however,the effectiveness and long-term toxicity need to be further studied and evaluated.

Objective:To prepare fluorouracil-loaded chitosan nanoparticle (5-Fu-CS-NP) for ophthalmologic usage and evaluate its releasing characteristics in vitro. Methods: 5-Fu-CS-NP was prepared with 5-Fu, polyacrylic acid and chitosan using dispersion method. The mean size, entrapment efficiency (EE%), drug loading (DL%) and the in vitro releasing characteristics of 5-Fu-CS-NP were investigated.Results and conclusion: The mean size of 5-Fu-CS-NP was (144.6?3.1) nm, the EE% was 84.5% and the DL% was 3.91%. 5-Fu-CS-NP had a satisfactory sustained-releasing effect and the releasing could last for 3-7 days, and the release behavior was not greatly changed within a pH value of 7.2-7.4.

Objective To study clinical features of patients with Parkinson's disease (PD)with chronic pain and its correlation with cognitive function.Methods 100 PD patients with chronic pain were selected as the observa-tion group.100 PD patients without chronic pain were selected as disease control group.90 healthy subjects with pain were selected as control group.Statistical and clinical features of PD patients with pain,and the relationship between cognitive function were made.Results 100 patients with chronic pain with PD skeletal muscle pain in 42 cases (42.0%),motility pain in 23 cases (23.0%),23 cases of nerve root pain (23.0%),9 cases of central pain (9.0%),akathisia in 3 patients (3.0%);Control pain in patients with chronic pain with part mainly in the head and neck and the waist,PD patients with chronic pain with mainly in the limbs,pain in the two groups the incidence of head and neck pain,waist pain,limb pain had statistical differences (χ2 =10.017,4.990,21.770,all P <0.05);Observation group of daily life,emotion,ability to walk,normal work,sleep,life fun scores were better than those in control group,with statistical significance (t =7.021,7.011,3.167,7.016,7.014,8.036,all P <0.05);PD with pain group and PD without pain in gender,age,course of the disease symptoms,PD with pain group and PD without pain in UPDRS score,H -Y in installment,HAMD scores,PDQL score,LED aspects comparison was statistically significant (χ2 =5.307,4.880,all P <0.05),PD with pain group smell disorders,motion sickness were higher than the proportion of the PD without pain group,with statistical significance (t =-1.689,-1.561,all P <0.05);PD patients with pain group named ability score,delayed recall below PD without pain group,the difference was statistically significant (P <0.05),PD patients with pain and PD without pain group in the MoCA scores and other project score had no statistical significance (t =-0.967,-0.272,-0.791,-0.837,-0.671,-0.658,all P >0.05).Conclusion PD associated with chronic pain is given priority to with skeletal muscle pain,PD associated with chronic pain pain is different from normal people,PD associated with chronic pain has influence on daily life, sleep quality and depression of patients bad feelings,PD associated with chronic pain are closely related to the depres-sion and cognitive dysfunction of patients.Cognitive and emotional disorders may be involved in the pathogenesis of chronic pain.

Objective:To observe the clinical effect of piribedil and madopar on Parkinson's disease ( PD) . Methods:Totally 58 patients with PD were divided randomly into the treatment group (29 cases) and the control group (29 cases). Piribedil and madopar were used in the treatment group, while only madopar was applied in the control group. Therapeutic effect of both groups was evaluated by UPDRS at the end of 6 courses. Results:The decrease of UPDRS after the remedy in the treatment group was more significant than that in the control group (P<0. 05), and the total effective rate in the treatment group(89. 7%) was significantly higher than that in the control group(65. 5%)(P<0. 05). Conclusion:The clinical effect of piribedil combined with madopar on PD is superior to that of madopar alone, therefore, the combination use is a useful method in the treatment of PD with the value of popularization and applica-tion.