S100A8 and S100A9 are abundantly expressed in neutrophils cytoplasm,they are calciumbinding proteins and they often exist as S100A8/A9 heterodimer.Previous studies have shown that the biological functions of S100A8 and S100A9 are associated with chronic inflammatory diseases and a variety of cancers.They are important to inflammation by binding and activation Toll-like receptor4 (TLR4) and receptor for advanced glycation end products(RAGE),and mediating intracellular inflammatory signaling transduction.This review summarizes the studies on functions and molecular mechanism of S100A8 and S100A9 in autoimmune diseases,which might propose new strategies for diagnosis,treatment and suggested disease activity.

Objective To evaluate the feasible of dual-energy coronary artery CTA combined with affirmed iterative reconstruction technique for overweight patients.Methods 60 cases underwent dual-energy coronary artery CTA were enrolled in this reasearch.Patients were divided into two groups according to different reconstruction arithmetic model:group A with FBP reconstruction model,while group B with SAFIRE reconstruction model and monoenergetic images from 65 to 90 keV (with increment of 5 keV).All the data were measured by one doctor worked in CT department for more than 5 years,and the image quality were analyzed and evaluated by two senior radiologists.Randomized block ANOVA analysis was used to compare objective evaluation indices of different monoenergetic images from 65 to 90 keV to get the best keV level.The comparative t-test was applied to objective evaluation indices(CT values,SD,SNR,CNR and image quality score) of group A and B and comparative x2-test was applied to image quality score at the best keV level.Results There were significant differences in CT value,SD,SNR,CNR of different keV images (P ＜0.05).For objective evaluation of image quality,the SNR and CNR of group B were significantly higher than group A,and SD was significantly lower than group A (P＜0.05).For subjective evaluation of image quality,there were statistically significant difference between group B and group A (P＜0.05),the segment score of group B was superior to group A.Radiation dose:the DLP value was (238.7±30.82) mGy · cm,and the ED value was (3.3±0.43) mSv.Conclusion SAFIRE combine with purified single spectrum technique can improve the image quality and reduce radiation dose in dual-energy coronary artery CTA,and 75 keV is the best single energy level.

Arthritis, Juvenile ; diagnosis ; Child ; Forecasting ; Humans ; Immunologic Tests ; Lupus Erythematosus, Systemic ; diagnosis ; Mucocutaneous Lymph Node Syndrome ; diagnosis ; Rheumatic Diseases ; diagnosis ; immunology ; therapy ; Rheumatic Fever ; diagnosis ; Scleroderma, Systemic ; diagnosis

Humans ; Macrophage Activation Syndrome ; diagnosis

The organic acidemia/aciduria is one of the most common inherited metabolic disorders in clinic,more than 50 species have been found until now.The illness is believed to be caused by gene mutation,leading to the reduction or loss of enzyme activity and the accumulation of carboxylic acid and its metabolites.The manifestations of increased blood organic acids include refractory metabolic acidosis,paroxysmal vomiting,feeding difficulties,hypotonia,convulsions and disturbance of consciousness.Most of the organic acidemia begins in neonatal period or infancy,accompanied by progressive neurological damages at most of the time.There are little specific clinical features can be found in this kind of diseases,therefore,early diagnosis and treatment must be initiated in order to decrease risk of neurological induries and damages or acute deaths.So application of the gas chromatography-mass spectrometry and tandem mass spectrometry is important to the early diagnosis,helpful for improving the outcomes and reducing child mortality.

Urea cycle disorder is a common inherited metabolic disorder,and it is the most common genetic cause of hyperammoniema in children.The illness is believed to be caused by gene mutation of six main enzymes in urea cycle,leading to ammonia,which is produced by amino acid catabolism,can't conver to urea through the urea cycle and be discharged through the urine.The manifestations of hyperammonemia turn out to be disorders of brain function (refusing to milk,vomiting,drowsiness,coma,convulsions,ataxia,aggressive behaviors).The incidence of this disease is 1/30 000.At the same time,the severity of the clinical symptoms is connected with the extent of the enzymes defects.More serious the enzymes defected,the earlier and worse the disease onsets.Some children with mild enzyme defects may intermittently attack or have a delay onset.Serious nervous system injuries can be found in hyperammonernia,therefore,early diagnosis and treatment must be ensured in order to decrease risk of mental injuries and damages or acute deaths.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

Objective To evaluate the reliability of using imipenem,meropenem,cefoperazonesulbactam,piperacillin-tazobactam in the treatment of hospital-acquired Gram-negative bacterial infections with Monte Carlo simulation(MCS).Methods The MIC of the four agents collected from hospital-acquired infections were detected in accordance with broth dilution method of Clinical and Laboratory Standard Institute (CLSI).MCS were conducted with MICs and the pharmacokinetics parameters of the four agents based on conventional dose regimens.The cumulative fraction of response (CFR) of time over MIC target attainment in different dosing regimen were generated.Results A total of 2 541 strains,including 2 093 strains of Enterobacteriaceae and 448 strains of glucose non-fermentative bacilli were collected.The MIC90 of imipenem and meropenem against Enterobacteriaceae were less than 1 mg/L in general,whereas MICg0 of two agents with β-1actamase inhibitors was around 64 mg/L.As to glucose non-fermenting bacteria,MICs of all the four agents were very high,especially to Acinetobacter baumannii,which indicated MIC50 more than 32 mg/L.MCS revealed that carbapenems had significantly higher CFR than those with β-1actamase inhibitors.Imipenem and meropenem (1 g,q8 h) obtained CFRs of 74.69％ and 81.42％,respectively.The CFR of cefoperazone-sulbactam (2 g,q8 h) and piperacillin-tazobactam (4 g,q6 h) (both excluding β-1actamase inhibitors) were just 49.59％ and 27.66％ respectively,which increased after excluding A.baumannii in piperacillin-tazobactam.Conclusions The conventional dose regimens of imipenem and meropenem are reliable for the empiric therapy of Gram-negative hospital-acquired bacterial infections.Piperacillin-tazobactam is suggested to use with higher doses or prolonged infusion time to satisfy the time of drug concentration exceeded the MIC (T ＞ MIC) requirement.More clinical studies of cefoperazone-sulbactam should be conducted to optimize its regimen and guarantee its efficacy.

Objective To compare the therapeutic efficacy and adverse effects between transcather arte-rial chemoembolization(TACE) and comprehensive therapy in primary advanced heptocellular carcinoma(HCC).Methods 216 patients with primary HCC were arranged randomly into two groups:104 (group A) were treated with TACE alone,the rest(group B)were treated with TACE combined with intraperitoneal perfusion chemothera-py, Chinese herbs and immunotherapy.The therapeutic efficacy and adverse effects were analysed and compared between two groups.Results The CR + PR rates were 28.84% in group A and 34.82% in group B.The 4-year survival rates were 1.92% in group A and 3.87% in group B(P < 0.05).There were no severe side effects in both groups.Conclusions The short-term effective rate and 4-year survival rate in comprehensive treatment group were superior to the control group.Therefore, the comprehensive treatment is worthwhile to use in primary advanced hepatocellular carcinoma.

Objective To assess the incidence rate of postoperative nausea and vomiting (PONV) after total intravenous anesthesia (TIVA) with propofol versus sevoflurane-based anesthesia with or without ondansetron in patients undergone gynecological laparoscopy. Methods Totally 138 patients were randomly divided into three groups: sevoflurane group (Sev group) sevoflurane-ondansetron group (Sev-O group),and propofol TIVA group (TIVA group),with 46 cases in each. In the Sev and Sev-O groups,anesthesia was maintained by inhalation of 50% N2O and sevoflurane;in the Sev-O group,8 mg of prophylactic ondansetron was given intravenously 30 minutes prior to the end of the operation. In the TIVA group,target-controlled infusion of propofol and remifentanil were used. In each group,the incidence of nausea and vomiting and use of antiemetic drugs in 24 hours after the surgery were recorded. Results In 0 to 2 hours after the operation,the incidence rate of nausea and vomiting in the TIVA group was significantly lower than that in the Sev group [22% (10/46) vs 54% (25/46),?2=10.376,P=0.001] and Sev-O group [50% (23/46),?2=7.986,P=0.005]. In 2-6 hours,the rate in the Sev-O group was lower than that in the Sev group [22% (10/46) vs 46% (21/46),?2=5.887,P=0.015]. The total incidence rate of nausea and vomiting in 24 hours after the operation was 57% (26/46) in the TIVA group,which was significantly lower than that in the Sev group [80% (37/46),?2=6.093,P=0.014]. In the Sev,Sev-O,and TIVA groups,there were 13 (28%),6 (13%),and 6 (13%) cases respectively received antiemetic drugs after the surgery. Conclusions Compared with sevoflurane-based anesthesia,propofol TIVA results in a lower rate of PONV after gynecological laparoscopy.