Compared to portal vein embolization and traditional two-stage hepatectomy,associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) can induce the proliferation of liver rapidly,concomitantly with high incidence of complications and mortality in the perioperative period.The feasibility and safety have been improved gradually as the improvement of technology and the accumulation of experience.But it is still controversial on its efficacy for malignant tumors,especially with insufficiency of medium-and long-term out-comes.The mechanism of rapid proliferation induced by ALPPS needs more studies with further steps.

Objective To analyse effect of different dose of sustained-release theophyline on prealbumin, C-reactive protein and white blood cell count in patients with pediatric asthma.Methods 58 children with acute asthma attack in our hospital were collected.All children were randomly divided into high dose group and low dose group,29 cases in each group.On the basis of conventional treatment, high dose group were treated with high dose theophylline sustained release tablets 5 mg/kg, one times per eight hours,orally,and low dose group was treated with low dose theophylline sustained release tablets,2 mg/kg, one times per eight hours,orally.Two groups were treatment for two weeks.After the treatment, the serum levels of pre albumin, C-reactive protein and veinal blood level of white blood cell count were detected in all children.Results Compared with high dose group post-treatment, the serum level PA was higher in low dose group (P<0.05);the serum level of CRP was lower in low dose group (P<0.05);the veinal blood level of WBC, percentage of neutrophils and percentage of eosinophils was lower in low dose group (P<0.05) .Conclusion Compared with high dose of sustained-release theophyline,the low dose of sustained-release theophyline can significantly reduce the serum CRP level and veinal blood level of WBC, percentage of neutrophils and percentage of eosinophils, improve serum PA level in patients with pediatric asthma, reduce inflammation in patients.

Objective\ To exam the expression and the distribution of the aromatase mRNA in the brain of the mouse. Methods\ RNA dot\|blotting as well as in situ hybridization technique were used. Results\ (1)There were aromatase specific mRNA expression in the brain tissue during the period from E16 to P300,the highest levels of mRNA were detected at postnatal 6 days,and the lowest levels were found at adulthood.(2)The location of the aromatase mRNA was confined to neuronal(but not glial)cell bodies and their processes.(3)The mainly distribution of aromatase mRNA was detected in the regions of the cerebral cortex,thalamus,hypothalamus and limbic system.Many heavily labeled cells were found in the layer of pyramidal cells of cerebral cortex,medial preoptic area.medial septal nucleus,pyramidal layer of hippocampus,amygdaloid nuclei.cingulate cortex,piriform cortex and periamygdaloid cortex.The moderately dense signal was present in several thalamic and hypothalamic nuclei such as ventromedial nucleus,ventrolateral nucleus,laterodorsal thalamic nucleus,paraventricular nucleus,etc. Conclusion\ There was relationship between the gene expression of aromatase with the development of brain,there was good agreement between the distribution of aromatase mRNA and aromatase activity as previously reported.The high levels of aromatase mRNA in the region of hippocampus and cerebral cortex suggested that aromatase may implicate for sex dimorphism in cognition as well as learning and memory.\;

Objective To explore the gene expression of aromatase and estrogen receptor (ER-?) in malignant glioma cell line SHG-44. Methods Cell culture, immunocytochemistry, in situ hybridization and RT-PCR techniques were used. Results Aromatase and estrogen receptor gene expressions were detected in SHG-44 cells.The aromatase gene in these cells was expressed by means of the multi promoters (1^3, 1^4 and P Ⅱ).Conclusion It may provide some new data for the hormone regulation in carcinoma of nerve system.

Objective To explore the effect and adverse reaction of topiramate(TPM) on treating Lennox-Gastaut syndrome(LGS)(including therapeutic alliance or single ). Methods Twenty-four cases with LGS whose attacks could not be controlled by re-(gular) therapy were selected.TPM was gradually increased from low dosage till its showing effect or untolerant adverse reaction.Results Two cases were excluded because of adverse reaction and increase of attacks. The remained cases were followed up from 6 months to 15 months (average: 9 months). The total effective rate was 82.6%, 11 cases accounting for 45.8% free of attack. The tonic-clonic seizure reduced more than 50% accounting for 82.2%, the full control accounting for 66.7%. The myoclonic seizure reduced more than 50% accounting for 81.8%,the full control accounting for 58.8%.The atypical absence seizure reduced more than 50% accounting for 81.8%, the full control accounting for 63.6%. The maximum effect occurred about 2-40 weeks following TPM used, the dosage about 2-10 mg/(kg?d).The adverse reaction included anorexia (8 cases), language disorder (5 cases), drowsiness (4 cases), decrease of anamnesis (3 cases), weight loss or unchanged(3 cases), inattention (3 cases), depression (3 cases), mental bradypraxia (2 cases ), skin damage (1 case), stupor (1 case), gross hematuria(1 case).The hepatic and renal function were normal during therapy. Conclusion TPM is a new, broad-spectrum, effective and safe antiepileptics drug on treating LGS.

China ; Disasters ; Earthquakes ; Emergency Medical Services ; Humans ; Public Health

Chorionic Villi ; metabolism ; pathology ; ultrastructure ; Diabetes, Gestational ; metabolism ; pathology ; Female ; Glucose ; metabolism ; Glycogen ; metabolism ; Humans ; Lipids ; analysis ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Placenta ; metabolism ; pathology ; ultrastructure ; Pregnancy

Continuous Positive Airway Pressure ; Facial Bones ; abnormalities ; Humans ; Infant ; Larynx ; pathology ; Oxygen Inhalation Therapy ; Pharynx ; pathology ; Polysomnography ; Respiration ; Risk Factors ; Sleep Apnea Syndromes ; diagnosis ; etiology ; therapy ; Sleep Apnea, Obstructive ; diagnosis ; etiology ; therapy

Objective To investigate the effect of meridian massage on the growth and development of premature infants.Methods 60 preterm infants were randomly divided into the observation group and the control group with 30 cases in each group,the control group adopted modified domestic simple meridian massage method,and the observation group increase meridian massage based on the control group,2 times a day and 15 minutes one time.Body weight,length,intake of milk,sleep time,time to regain birth weight,and meconium drained time were eomparel between two groups.Results The observation group was significantly better than the control group in body weight,length,intake of milk,sleep time,time to regain birth weight,and meconium drained time.Conclusions Meridian massage based on the domestic simple touch method plays a significant role in promoting the growth and development of premature infants.

A quantitative method using ultrahigh-performance liquid chromatography was established to simultaneously determine ten ginsenoside active ingredients including ginsenoside Rg6, F4, Rk3, Rh4, 20(S) -Rg3, 20(R) -Rg3, Rk1, Rg5, 20(S)-Rh2 and 20(R)-Rh2 in steamed notoginseng. The ten ginsenosides of steamed notoginseng with different head numbers, parts, and steaming time were determined by this method. An Acquity BEH C18 chromatographic column (2.1 mm x 100 mm, 1.7 microm) was used to perform the determination, which was maintained at 35 degrees C throughout the analysis. Mobile phase was composed of water and acetonitrile with flow rate at 0.3 mL x min(-1) under gradient elution, and detection wavelength was set to 203 nm for monitoring the separation. The results demonstrate ginsenoside Rg6, F4, Rk3, Rh4, 20 (S)-Rg3, 20 (R) -Rg3, Rk1, Rg5, 20 (S)-Rh2 and 20(R) -Rh2 have shown good linearity (R2 > or = 0.999 8) within 0.46-115, 2.06-515, 1.632408, 3.216-804, 1.392-348, 1.4-350, 0.496-248, 3.012-1 506, 0.82-205 and 0.832-208 mg x L(-1), and their average recoveries were 97.00%, 97.96%, 98.86%, 95.27%, 98.67%, 98.02%, 95.53%, 96.63%, 99.57% and 103.6%, respectively. The proposed approach was quick and accurate and portrayed excellent repeatability and determination efficiency. The quality of steamed notoginseng was effectively controlled, which served as a foundation for establishing a normalized processing technique and quality standard for ensuring the reliability and consistency of its clinical efficacy.
Chromatography, High Pressure Liquid ; methods ; Ginsenosides ; analysis ; Panax notoginseng ; chemistry ; Reproducibility of Results ; Steam