1.Constructing scientific training quality assurance system to ensure the training quality of master of public health (MPH)
Ruiquan FAN ; Weihong DAI ; Dexiu GAN ; Xiao PENG ; Wen CHEN
Chinese Journal of Medical Education Research 2002;0(01):-
On the basis of the summarization about some MPH education experience in Sun Yat-sen University,we make the assumption concerning construction of MPH training quality assurance system ,expound the content and operating mode of this training quality system,and then offer reference for improvement of MPH training quality.
2.Preparation and identification of scFv and bsFv against transferrin receptor.
Jing, LIU ; Daiwen, XIAO ; Xiaoou, ZHOU ; Xue, WEN ; Hong, DAI ; Zhihua, WANG ; Xin, SHEN ; Wei, DAI ; Daofeng, YANG ; Guanxin, SHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2008;28(6):621-5
To obtain single chain variable fragment (scFv) and bivalent single chain variable fragment (bsFv) against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy (VH) and FR4 of variable light (VL), respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv.
Base Sequence
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Cloning, Molecular
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Escherichia coli/genetics
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Escherichia coli/metabolism
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Genetic Vectors/genetics
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Hep G2 Cells
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K562 Cells
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Molecular Sequence Data
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Receptors, Transferrin/*immunology
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Recombinant Fusion Proteins/biosynthesis
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Recombinant Fusion Proteins/genetics
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Single-Chain Antibodies/*biosynthesis
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Single-Chain Antibodies/genetics
3.Value of urinary retinal binding protein in early renal function impairment for patients with pneumoconiosis complicated with chronic obstructive pulmonary diseases.
Jun-he DAI ; Si-hai LIU ; Xiao-jing LIU ; Li-da YAN ; Wen-shou XU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(2):123-124
4."Outcomes in patients with ""successful"" recanalization in anterior circulation stroke treated with mechanical thrombectomy: TICI 3 versus TICI 2b"
Fengli LI ; Jinjing WANG ; Zheng DAI ; Feng PENG ; Lulu XIAO ; Wen SUN ; Min LI ; Xinfeng LIU
International Journal of Cerebrovascular Diseases 2017;25(5):406-411
ObjectiveTo investigate the difference in the outcomes in patients with acute anterior circulation stroke having achieved the Thrombolysis in Cerebral Infarction (TICI) 2b and TICI 3 recanalization after mechanical thrombectomy.MethodsThe patients with acute anterior circulation stroke having achieved successful recanalization after mechanical thrombectomy were enrolled retrospectively.They were divided into TICI 2b (almost complete recanalization) group and TICI 3 (complete recanalization) group.The good outcome was defined as the modified Rankin Scale score 0-2 at 3 months after onset.ResultsA total of 83 patients were enrolled in the study, including 38 patients (45.8%) with TICI 2b and 45 (54.2%) with TICI 3;49 (59.0%) had good outcome, and 34 (40.9%) had poor outcome.The good outcome rate in the TICI 3 group was significantly higher than that in the TICI 2b group (68.9% vs.47.4%;χ2=3.946, P=0.047).After adjusting for age, hypertension, diabetes, baseline systolic blood pressure, triglyceride, intravenous thrombolysis, and ASITN/SIR collateral grades, TICI 3 was an independent predictor for good outcome at 3 months after onset (odds ratio [OR] 3.759, 95% confidence interval [CI] 1.098-12.871;P=0.035), while the higher baseline National Institutes of Health Stroke Scale score (OR 0.820, 95% CI 0.715-0.941;P=0.005) and higher fasting glucose (OR 0.610, 95% CI 0.410-0.906;P=0.014) were the independent predictors for poor outcome at 3 months after onset.ConclusionsThere are difference in the outcomes in patients with successful recanalization in anterior circulation stroke treated with mechanical thrombectomy.The outcome is substantially better in TICI 3 than TICI 2b patients.
5.Clinical study on mild moxibustion for knee osteoarthritis
Ming DAI ; Xiao-Yan FANG ; Hui-Yi CHEN ; Yan-Hong WANG ; Yi-Wen WU
Journal of Acupuncture and Tuina Science 2019;17(1):62-66
Objective:To observe the clinical effect of mild moxibustion for knee osteoarthritis (KOA) and to explore the role of mild moxibustion in relieving pain,reducing stiffness and improving joint dysfunction in patients with KOA.Methods:Eighty patients with KOA were randomly allocated into either a moxibustion group or a medication group by the random number table,with 40 cases in each group.The moxibustion group used mild moxibustion at Neixiyan (EX-LE 5),Dubi (ST 35),Xuehai (SP 10) and Liangqiu (ST 34),30 min each time,3 times a week;the medication group was given celecoxib capsule (celebrex),0.2 g each time,once a day.Both groups were treated for 4 weeks.The visual analog scale (VAS) and Western Ontario and McMaster Universities osteoarthritis index (WOMAC)scores were evaluated before and after treatment.The efficacy of the two groups was compared after treatment.Results:After treatment,the overall efficacy of the moxibustion group was significantly different from that of the medication group (P<0.05).The VAS and WOMAC scores of the two groups were lower than those before treatment (both P<0.01).The changes in the VAS and WOMAC scores after treatment in the moxibustion group were significantly different from those in the medication group (both P<0.05).After treatment,in single item of WOMAC,the changes in pain and joint dysfunction in the moxibustion group were more statistically significant than those in the medication group (both P<0.05).Conclusion:Mild moxibustion and oral celebrex can reduce the VAS and WOMAC scores of patients with KOA.Mild moxibustion is superior to oral celebrex in relieving pain and improving joint function.
6.Epidemiological characteristics of influenza in Mianyang, Sichuan, 2019-2021
WEN Yan ; CHEN Hua ; LIU Shi-an ; YANG Xiao-rong ; CHEN Guo ; DAI Yu-ke
China Tropical Medicine 2023;23(1):64-
Objective To understand the epidemiological and etiological characteristics of influenza in Mianyang City
from 2019 to 2021, so as to provide a basis for the prevention and control of influenza. Methods Influenza surveillance data
in Mianyang City from 2019 to 2021 were collected and analyzed statistically. Results A total of 55 970 cases of influenza
were reported in Mianyang City from 2019 to 2021, with an average annual incidence of 388.08/100 000. A total of 103 723
cases of influenza -like illness cases (ILI) were reported, with an average annual ILI% of 3.58%. The incidence, ILI% , and
positive detection rates of influenza were all far higher than those in the corresponding period in 2019. The classification of the
population is mainly composed of students under the age of 15. The top three reported cases were Fucheng District (20 118,
35.94%), Youxian District (6 394, 11.42%) and Jiangyou District (5 800, 10.36%). 10 126 samples of ILI were received and
detected, with a positive rate of 19.53%, the positive rate of ILI samples was mainly students under 15 years old. The dominant
strains of influenza viruses showed an alternating trend over the years, and A (H3) was the predominant type in 2019. Except for
2 A (H9) strains detected in 2021, the rest were all BV strains. Due to the impact of COVID-19 in 2020, the positive detection
rate was low throughout the year. 43 outbreaks of ILI were reported, which were mainly occurred in winter, and most of them
were in primary schools. Conclusion From 2019 to 2021, the characteristics of cases, ILI, pathogen surveillance and
outbreak events of influenza in Mianyang City are basically the same, with students under 15 years of age and schools
remaining the key population and sites of concern. the importance of non-pharmaceutical interventions for influenza prevention
and control is further evidenced by the low incidence of influenza during the COVID-19 pandemic.
7.Clinical observation of gefitinib in treatment of female patients with adenocarcinoma of lung WU
Xiao-Ping WU ; Ying-Zhi ZHUANG ; Hao JIANG ; You-Hua WU ; Wen-Xiang DAI ; Xiao-Hong AI ; San-Yuan TANG ;
Cancer Research and Clinic 2006;0(12):-
Objective To evaluate the efficacy and adverse effects of gefitinib in the treatment of fe- male patients with advanced adenocarcinoma of lung who had failed to previous chemotherapy.Methods These patients received 250mg of gefitinib orally,once daily until disease progression or development of intol- erable toxic reaction.They were evaluated one month after treatment and every other month thereafter.Results Among the 27 evaluable patients,there were 1 CR(3.7%),11 PR(40.8%),10 SD(37.0%)and 5 PD(18.5%). The overall response rate was 44.5%(95% CI 29%~68%);and 22 patients(81.5%)gained profit(CR+PR+ SD)from the clinical therapy(95% CI 62%~94%);the mean TTP was 7.2 months.Symptomatic improvement rate was 80.0%.The main adverse effects were mild rash and diarrhea.Conclusion gefitinib has significant efficacy in the treatment of female patients with advanced tung cancer who had failed to previous chemother- apy.Adverse effects are mild.gefitinib is a suitable therapy for these patients.
8.Clinical features and outcome analysis of 83 childhood Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis with HLH-2004 protocol.
Li XIAO ; Ying XIAN ; Bi-tao DAI ; Yong-chun SU ; Jian-wen XIAO ; Qi-cheng ZHENG ; Xiao-dong ZHAO ; Jie YU
Chinese Journal of Hematology 2011;32(10):668-672
OBJECTIVETo investigate the clinical features of Epstein-Barr virus-related hemophagocytic lymphohistiocytosis (EBV-HLH), to analysis the outcome of HLH-2004 protocol, and to explore the prognostic factors in EBV-HLH patients.
METHODSThe clinical features at onset and outcome of HLH-2004 protocol from 83 pediatric patients with EBV-HLH enrolled from January 2006 to December 2009 in our hospital were analyzed retrospectively. Univariate and multivariate COX regression analysis were used to identify statistically significant prognostic factors.
RESULTS(1) Among the 83 patients, 45 were males and 38 were females. The age of onset ranged from 6 months to 14 years 4 months. 44 patients were treated with HLH-2004, and 3-year overall survival (OS) was (55.8 ± 7.9)%. (2) The most common clinical features of EBV-HLH included high fever, cytopenia, hepatosplenomegaly, and coagulopathy; The respiratory symptoms, angina phlogistic, skin rashes, neurologic abnormality were rare. 97.3% of patients showed an elevation of serum ferritin, liver dysfunction and lipid metabolism disorders was found in most of EBV-HLH patients. 89.0% of patient had hemophagocytosis in bone marrow at diagnosis of EBV-HLH. (3) COX regression analysis revealed that anemia degree, serum albumin < 30 g/L, CD4:CD8 abnormity, NK cell < 3%, treatment protocol were related with the prognosis significantly (P < 0.05).
CONCLUSIONEBV-HLH in pediatric patients has severe clinical feature and poor prognosis. HLH-2004 protocol is an effective treatment for patients with EBV-HLH. Symptomatic treatment can't rescue the patients of EBV-HLH.
Adolescent ; Child ; Child, Preschool ; Epstein-Barr Virus Infections ; drug therapy ; Female ; Herpesvirus 4, Human ; Humans ; Infant ; Killer Cells, Natural ; Lymphohistiocytosis, Hemophagocytic ; drug therapy ; virology ; Male ; Prognosis ; Retrospective Studies ; Treatment Outcome
9.Effect of total saponins from Sanguisorba officinalis on megakaryocyte progenitor cells proliferation, differentiation and relative receptor expression.
Yan-Ping DAI ; Xiao-Ping GAO ; Jian-Ming WU ; Xiang LI ; Fei-Hong HUANG ; Wen-Jun ZOU
China Journal of Chinese Materia Medica 2014;39(9):1685-1689
OBJECTIVETo investigate the effects of total saponins from Sanguisorba officinalis (DYS) on hematopoietic cell proliferation, differentiation and the expression level of IL-3R and c-kit.
METHODBaf3 and 32D cells were cultured with or without IL-3, then the cells were exposed to DYS in different concentrations of 5, 10, 20, 30 and 40 mg x L(-1) for 24, 48, 72 and 96 hours separately. After that, the cell proliferation and differentiation capacity were determinated by the methods of CCK8 and Giemsa staining separately. The effects of DYS on the expression level of IL-3 receptor in Baf3 cells and the expression level of c-kit in 32D cells were determinated using RT-PCR.
RESULTDYS promotes alone proliferation of Baf3 cells and 32D cells after 48 h. In contrast to control cells, 32D cells containing DYS without IL-3 form many large clusters. DYS also increases the proliferation when cultured with IL-3. High concentration of DYS induce alone the differentiation of 32D cells and increase alone the number of the polyploidy megakaryocyte. Moreover, DYS increases alone the expression level of IL-3R in Baf3 cells and the expression level of c-kit in 32D cells separately.
CONCLUSIONOur data shows DYS can promote alone proliferation and differentiation of megakaryocyte progenitor cells. The proliferative and differentiative effect of DYS on megakaryocyte progenitor cells is correlated to the up-regulation of IL-3 receptor and c-kit expression.
Animals ; Cell Differentiation ; drug effects ; genetics ; Cell Line ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Gene Expression ; drug effects ; Interleukin-3 ; pharmacology ; Megakaryocyte Progenitor Cells ; drug effects ; metabolism ; Mice ; Proto-Oncogene Proteins c-kit ; genetics ; Receptors, Interleukin-3 ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sanguisorba ; chemistry ; Saponins ; pharmacology ; Time Factors
10.Therapeutic effect of "shen-bing N05" on adenine-induced chronic nephritis in rats
Quan-Rong ZHOU ; Shi-Wen DAI ; Xu-Ping WANG ; Wei-Min ZHANG ; Shu-Yan XU ; Cheng-Wei XIAO ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim The therapeutic effect of "shen-bing NQ 5", a preparation of traditional Chi-nese medicine on adenine-induced chronic nephritis in rats was studied. MethodsChronic nephritis in rats was induced by giving adenine 80 mg?kg-1?d-1 orally. Themodel rats were treated by "shen-bing No 5" in different dosages. Levels of urea ni-trogen(BUN), creatinine (Cr), total protein(TP), and Na+ in serum and or urine weredetected and the changes of kidney were obseraed. Results The "shen-bing No 5"markedly lowered the levels of urine protein in 24 hours, BUN in urine and BUN, Na+in serum (P