Objective To observe the therapeutic effect of red peony root decoction (RPRD) on model rats with acute pancreatitis (AP),and the effect on the expression of NF-κB,TNF-α and IL-6.Methods Forty SD rats were randomly divided into five groups:normal control group (group A),AP model groups (group B,24 h; group C,72 h) and RPRD treatment groups (group D,24 h; group E,72 h).There were 8 rats in each group.The AP models were induced by intraperitoneally injecting with L-arginine.After the model was established,rats of group B and group C were treated with intra-gastric instillation of NS,and rats in group D and group E with RPRD.At 24 and 72 hours after induction of AP,rats in each group were sacrificed and blood samples were taken for the measurement of serum amylase,TNF-α and IL-6.The expression of NF-κB p65 protein in the pancreas were detected by Western Blot,and pathological status of the pancreas were analyzed.Results The serum amylase level in group B and group D was significantly higher than that in group A (P＜0.05),and the level in group D was obviously lower than that in group B (P＜0.05).The serum amylase level in group C and group E was decreased,and there was no difference between group A (P＞0.05).The pathological severity and pathological scores of the pancreas in group D and group E were more lighter than those in group B and group C (P＜0.05).The level of serum TNF-α and IL-6 was significantly decreased in RPRD treatment groups (P＜0.05),group D compared with group B,[ (12.54±2.12)pg/ml,(552.88±81.27)pg/ml] vs.[ (20.53±4.16)pg/ml,(1255.75± 118.82)pg/ml]; group E compared with group C,[ (11.01 ±2.02)pg/ml,(582.87± 88.86) pg/ml] vs [ (23.85 ± 5.59) pg/ml,(1366.33 ± 163.16) pg/ml].The expression levels of NF-κBp65 protein in the pancreas of group D and group E were significantly lighter than those in group C at both time points (group D compared with group B,(0.07±0.055) vs (0.71 ±0.029),P＜0.05; group E compared with group C,(0.15±0.038) vs (0.65±0.026).Conclusion RPRD may alleviate the pathologic changes in AP

Objective To observe the therapeutic effects of Radix Paeoniae Rubra decoction (RPRD) on a-cute pancreatitis (AP) in rats, and to study effects of RPRD on the expression of NF-κB, TNF-α and IL-6. Method Forty Sprague-Dawley (SD) rats were, randomly divided into five groups: normal control group (group A), AP model groups (group B, 24 h; group C,72 h) and RPRD treatment groups (group D, 24 h;group E,72 h).There were 8 rats in each group. The AP models were induced by intraperitoneally injecting with L-arginine. After the model was estabhshed, rats of group B and group C were treated with inn'a-gastric instillation of NS, and rats in group D and group E with RPRD. At 24 and 72 hours after induction of AP, rats in each group were sacri-riced and blood samples were taken for the measurement of serum amylase. The expression of NF-κB 1065 protein and TNF-α and IL-6 mRNA in the pancreas was detected by Western blotting and real-time quantitative PCR, re-spectively, and the pathological status of the pancreas was analyzed. Results The serum amylase level in group B and group D was obviously increased at 24 hours, and were significantly higher than that in group A (P < 0.05) ; the level in group D was lower thaa that in group B (P < 0.05). the level reduced at 72 hours, and there was no difference between group E and group C (P > 0.05). The pathological severity and the pathological score of pan-creatic tissue in group D and group E at 24 hours and 72 hours were more hghter than those in group B and hours C (P < 0. 05). The expression levels of NF-κB p65 protein (Western blotting) in the pancreas of group D and group E were significantly lighter than those in group B and group C at both time points (group D compared with group B, (0.07 ± 0.006) vs. (0.71 ± 0.029), P < 0.05;group D compared with B, (0.07 ± 0.006) vs. (0.71 ±0.029), P <0.05; group E compared with C, 0.18±0.014 vs. 0.65±0.026, P <0.05). The results of real-time quantitative PCR shown that the expression levels of TNF-α and IL-6 mRNA in model treatment group were significantly stronger than that in RPRD group at the two time points (P < 0.05). Conclusions RPRD is effective for the treatment of AP rats to inhibit the activity of NF-κB. Inhibition of NF-κB offers a promising strategy to treat AP by down-regulating eytokine expression.

Adaptor Proteins, Signal Transducing ; genetics ; Bone Marrow Diseases ; genetics ; pathology ; DNA Mutational Analysis ; DNA-Binding Proteins ; genetics ; Exocrine Pancreatic Insufficiency ; genetics ; pathology ; Glucose-6-Phosphatase ; genetics ; Humans ; Leukocyte Elastase ; genetics ; Lipomatosis ; genetics ; pathology ; Mutation ; Neutropenia ; congenital ; genetics ; Proteins ; genetics ; Transcription Factors ; genetics

To study the effect of total matrines and extracts from Periplaneta americana on negative endometrial cancer cell JEC of progesterone receptors. After detecting the effect of total matrine, extracts from P. americana and their combination on JEC cells' growth inhibition, cell cycle, P53 and c-erbB-2 gene protein expressions through MTT, flow cytometry instrument and Western blot method, the author found that, (1) MTT: total matrines and extracts from P. americana could inhibit the growth of JEC cell, with significant increase in the inhibitory effect in the combination group. (2) Flow cytometry instrument: the cell cycle at G0/G1 increased after the treatment with total matrines, the cell cycle at G2/M increased after the treatment with extracts from periplaneta americana, and the ratio of G0/G1 cell cycle in the combination group was significantly higher than the other groups, with inhibition in cell growth and statistical difference in inter-group comparison (P < 0.05). (3) Western blot: the expression level of P53 increased and c-erbB-2 decreased after the treatment with total matrines, extracts from P. americana and their combination on JEC cell, with statistical difference in inter-group comparison (P < 0.05). The above results suggested that total matrines, extracts from P. americana and their combination could induce cell cycle arrest and inhibit the growth of JEC cell by up-regulating P53 and down-regulating the c-erbB-2 level.
Alkaloids ; therapeutic use ; Animals ; Cell Line, Tumor ; Endometrial Neoplasms ; chemistry ; drug therapy ; pathology ; Female ; Flow Cytometry ; Humans ; Periplaneta ; Phytotherapy ; Plant Extracts ; therapeutic use ; Quinolizines ; therapeutic use ; Receptors, Progesterone ; analysis ; Tumor Suppressor Protein p53 ; analysis ; physiology

Hematopoietic Stem Cell Transplantation ; Hepatocyte Growth Factor ; pharmacology ; Humans ; Immunologic Factors ; pharmacology ; Postoperative Period

Examination paper analysis is an important job in examination management.It includes qualificative analysis and quantitative analysis,which are connective and complementary.This essay discusses the selection and application of examination paper analysis indexes.

Clinopodii Herba is a type of traditional folk medicine. Modern research showed that Clinopodii Herba contains triterpenes and its saponins, flavones, volatile oil, phenylpropanoids and other chemical components. It has many pharmacological activities such as hemostasis, antibiosis, hypoglycemic activity, anti-oxidation, and anti-in-flammation. The main clinical application of Clinopodii Herba is for the treatment of various types of bleeding. After searching literatures on Clinopodii Herba for the recent 20 years, this article reviewed the chemical components, pharmacological activities, clinical application and research condition of Clinopodii Herba in order to provide further references for this medicine.

Objective To investigate the effect of blocking VEGF expression on the radiation sensitivity of esophageal cancer cell line TE-1 in vivo. Methods 32 male Balb/c/nu nude mice were randomly divided into four groups, including control group, radiation group, anti-VEGF group, and anti-VEGF + radiation group. The anti-VEGFcDNA cells were subcutaneously injected into the paw pats of mice (2 × 106/100 μl). The subcutaneous tumors were irradiated with 18 Gy of 60Co y-rays when the diameter of tumors varied from 0. 8 to 1.0 cm. The volume of the tumors was measured before and after irradiation, respectively. The expression level of VEGF mRNA and protein were examined by RT-PCR and Western blotting, respectively. Apoptotic cells were detected by electron micrographs. Results Latent period of the tumor formation of anti-VEGF group was lengthened compared with other groups(t = 13. 898,P ＜0.01 ). The volumes of tumor in anti-VEGF group [ ( 1207. 50 ± 97.07 ) mm3 ] and anti-VEGF +radiation group [ ( 1057. 5 ± 91.50 ) mm3 ] were not statistically different post-irradiation ( t = 1. 124, P ＞0.05 ) , but smaller than those in control group [ ( 5442. 50 ± 185.08 ) mm3 ] and radiation group [ (2922. 50 ± 152. 773)mm3 ] with statistical differences( t = 9. 475-21. 238, P ＜ 0. 01 ). The expression level of endogenous VEGFmRNA and protein in anti-VEGF group and anti-VEGF + radiation group were statistically different from control group and radiation group (F = 387.394, 13.519, P ＜ 0.01 ).Conclusions Antisense VEGF could inhibit the proliferation of esophageal cancer cell in the nude mice.Effect of blocking VEGF expression before irradiation on esophageal cancer xenografts might be limited.

Animals ; Cells, Cultured ; Hepatocytes ; drug effects ; secretion ; Male ; Pancreatic Polypeptide ; pharmacology ; Rats ; Rats, Sprague-Dawley

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Male ; Mucopolysaccharidosis II ; genetics ; Pedigree