2.Comparison of MRI contrast agents gadopentetate dimeglumine, gadodiamide and gadovist: their relaxation rates and effects on imaging
Chinese Journal of Geriatrics 2016;35(9):993-995
Objective To evaluate the relaxation rates and imaging effects of three MRI contrast agents gadopentetate dimeglumine(0.5 mol/L),gadodiamine and gadovist(1.0 mol/L) in the nervous system.Methods Relaxation rate differences between the three contrast agents were assessed using the GE Signa HDx 3.0 T MR scanner and phantom solutions of different albumin concentrations.Twenty leukemia patients whose initial scans had been conducted with the injection of a standard dose(0.5 mol/L)of gadopentetate dimeglumine as the contrast agent were recalled to have a follow-up scan for signs of brain infections with the same imaging protocols,except that a high concentration(1.0 mol/L)gadovist was used this time as the contrast agent.CNR and SNR in the ROI were measured for quantitative analysis.Results Changes in dosage of the three contrast agents produced no difference in intensity of the image signal for each phantom solution of a specific albumin concentration(5.0 g/L:P=0.35,6.5g/L:P =0.27,8.0 g/L:P=0.23).Two sets of scans of the leukemia patients showed that high concentration(1.0 mol/L)gadovist generated higher SNR and CNR in the ROI of the white matter,gray matter and vasculature than standard concentration(0.5 mol/L) gadopentetate dimeglumine(P< 0.05).Conclusions A half dose of high concentration(1.0 mol/L) gadovist generates better imaging enhancement than standard concentration(0.5 mol/L)gadopentetate dimegluminethe.Gadopentetate dimeglumine,gadodiamide and gadovist have no significant difference in relaxation rate.
3.Construction and Identification of Recombinant Plasmid of p3XFLAG-CMV7-NICD1
Journal of China Medical University 2015;(3):217-220
Objective To construct the eukaryotic expression vector of Notch1intracellular domain,p3XFLAG?CMV7?NICD1,so as to prepare for the further research and exploration of effect of Notch1 on promoting epithelial?mesenchymal transition of human lens epithelial cells. Methods The cDNA fragment was reversely transcribed by RT?PCR from total RNA extracted from the SRA01/04 cells and was encoded with the specific am?plification?targeted NICD1was obtained from the SRA01/04 cells,then the cDNA fragment was inserted into p3XFLAG?CMV7 to transcribe Esche?richia coli DH5α. And the recombinant plasmid was extracted after bacterial screening by LB plating medium and confirmed by the restriction endo?nuclease digestion and DNA sequencing. Results The target gene obtained had the same molecular size as predicted. It was indicated that recom?bined p3XFLAG?CMV7 plasmid contained correct recombinant human Notch1 sequences and p3XFLAG?CMV7?NICD1 was constructed success?fully. The western blotting showed protein NICD1 expressed in SRA01/04 cells transfected with p3XFLAG?CMV7?NICD1. Conclusion The suc?cessful construction of p3XFLAG?CMV7?NICD1 will provide a foundation for a further study studies in on the effect relationship of Notch signaling pathway and in posterior capsular opacification(PCO)after cataract extraction.
4.Risk factors of acute organophosphorus pesticide poisoning
Xiao-Wei LIU ; Zhi LIU ;
Chinese Journal of Emergency Medicine 2006;0(05):-
Objective To explore the risk factors of acute organophosphorus pesticide poisoning(AOPP). Method The patients with acute organophosphorus pesticide poisoning,admitted from September 2004 to August 2005,were retrospectively analyzed.The 87 patients were divided into groups according to the presence or absence of combined conditions including hypotension,hypoxemia or metabolic acidosis.Acute physiology and chronic health evaluationⅡ(APACHEⅡ)score was calculated.The chi-square test was used to examine the mortality between those groups.Results The total in-hospital mortality of 87 acute organophosphorus pesticide poisoning patients was 21.8%.The mean APACHEⅡscore was(7.58?5.32)in the 68 survivors and(21.17~9.46)in the 19 dead,there were significant differences between the survivors and the dead(t=9.25,P20 was 65.2%(15/23),andit was 6.3% (4/64)in patients with APACHEII score
5.Determination of Triptolide and Wilforlide A in Biological Samples by LC-MS/MS.
Journal of Forensic Medicine 2015;31(6):445-453
OBJECTIVE:
To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and to verify the method.
METHODS:
After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl (100 mm x 2.1 mm, 5 µm) with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization (ESI⁺) in positive mode was elected and the data was collected using multiple-reaction monitoring (MRM).
RESULTS:
The linearity was good (r > 0.995 0) and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%.
CONCLUSION
This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.
Chromatography, High Pressure Liquid
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Diterpenes/urine*
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Epoxy Compounds/urine*
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Humans
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Oleanolic Acid/urine*
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Phenanthrenes/urine*
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Sensitivity and Specificity
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Tandem Mass Spectrometry
7.CD30-positive of diffuse large B-cell lymphoma of small intestine co-existing with tubular adenocarcinoma of rectum: report of a case.
Chinese Journal of Pathology 2007;36(9):641-642
Adenocarcinoma
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metabolism
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pathology
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surgery
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Aged
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Humans
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Intestinal Neoplasms
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metabolism
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pathology
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surgery
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Intestine, Small
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Ki-1 Antigen
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metabolism
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Lymphoma, Large B-Cell, Diffuse
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metabolism
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pathology
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surgery
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Male
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Neoplasms, Multiple Primary
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metabolism
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pathology
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surgery
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Rectal Neoplasms
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metabolism
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pathology
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surgery
8.Effect of Aitongxiao Granules Containing Serum on Proliferation and Apoptosis of SMMC-7721 and Bel-7404 Cells in Vitro
Yongqin ZHANG ; Xiao LIU ; Ailing WEI
Chinese Journal of Information on Traditional Chinese Medicine 2014;(3):58-61
Objective To observe the anti-cancer effect in vitro of Aitongxiao Granules containing serum on SMMC-7721 and Bel-7404 liver cancer cells, thus revealing the anti-cancer mechanism of the treatment in adjusting proliferation and apoptosis. Methods Male Sprague-Dawley rats were administered by gastrogavage Aitongxiao decoction of 86.4, 43.2, 21.6 g/kg twice a day for 1 week. Using serum pharmacologic method, the proliferation of SMMC-7721 and Bel-7404 liver cancer cells were determined by MTT chromatometry after co-cultured with medicated serum containing different concentration of Aitongxiao decoction. The apoptosis of SMMC-7721 and Bel-7404 liver cancer cells were detected by flow cytometry. Results The research in vitro showed that Aitongxiao Granules containing serum low, medium and high dose groups had varying degrees of inhibition on hepatoma cells, and this inhibition showed a concentration dependence within a certain range. Aitongxiao Granules containing serum could induce apoptosis of human hepatoma SMMC-7721 cells and Bel-7404 cells. Conclusion Aitongxiao Granules showed effect of inhibiting proliferation and inducing apoptosis on SMMC-7721 and Bel-7404 cells in vitro.
9.Methods of trasferral of tissues and cells on slices.
Chinese Journal of Pathology 2008;37(5):348-349
Adoptive Transfer
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methods
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trends
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Cells
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pathology
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Cells, Cultured
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pathology
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Humans
10.Analysis of the mutation of rhodopsin gene in an inbreeding family with autosomal recessive retinitis pigmentosa
Jing LIU ; Lin XIAO ; Wei WANG
Chinese Journal of Ocular Fundus Diseases 2001;0(03):-
Objective To detect characteristics and the pathogenesis of rhodopsin (RHO) gene mutation in an inbreeding family with autosomal recessive retinitis pigmentosa (ARRP). Methods Peripheral venous blood 5-8 ml was abstracted from 8 members in the inbreeding ARRP family and 10 control individuals. DNA gene group was picked. Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR),and the mutation of RHO gene was screened by direct DNA sequence measurement. Results The Gln-344-Arg mutation in the RHO gene was detected in 3 patients with ARRP and homozygotes of the mutation in 3 patients were found. Heterozygous of the mutation was detected in the parent of patients and 1 healthy family member. No mutation of RHO gene was found in 2 healthy family members and 10 control individuals. Conclusions The Gln-344-Arg mutation in the RHO gene may be the pathogenic factor of the ARRP family; the frequency of the mutation of RHO gene may increase in the inbreeding ARRP family.