Objective To investigate the relationship between the different embolization agent and the spinal cord ischemic injury. Methods Bilateral of intercostal arteries were embolized with gelatin sponge and lipiodol to block the blood supply of spinal cord. To detect the somatosensory evoked potentials(SEPs), 6 canines in both groups of gelatin sponge and lipiodol were treated by bilateral intercostal arteries embolization. The median nerve and fibular nerve were recorded and the ratio of lower extremity to upper extremity SEPs amplitude (LE/UE ratio) were calculated; the results were compared with the pathologic findings and the motor function. Results The baseline, LE/UE, evoked potential amplitude ratios in all dogs before operation ranged from 0.22 to 0.92 with a combined total mean of 0.49?0.07. The SEPs changes at 30 minutes post operation were more obvious than pre operation in gelatin sponge group and lipiodol group with statistic significance. The LE/UE ratio of gelatin sponge group recovered one hour after operation, but this was absent in lipiodol group. So did the latency SEPs changes. Conclusion The SEPs could not be used to evaluate the motor function of spinal cord comprehensively.

To further analyze immunoglobulin (Ig) properties of panel reactive antibodies (PRA) and their potential harmful effects on allogeneic renal transplants. Methods Since dithiothreitol (DTT) is able to crack disulphuric bond of Ig and to depoly-merize IgM with heavy molecular weight, PRA study was performed with treatment of DTT. Thus, a DTT-ERA method was established, which can identify immunoglobulin class in sera of high PRA patients. Results Among 701 recipients, whose sera were positive by the standard PRA, the positive rate of DTT-PRA was 33.2% . Besides, positive PRA patients could be divided into three groups according to their sera's sensitivity to DTT: Group 1 consisted of patients whose sera contained exclusively IgM antibodies; Group 2 consisted of patients whose sera contained of IgG antibodies only; Group 3 consisted of patients whose sera were found to contain both IgM class antibodies and IgG antibodies. Conclusion IgM class antibodies are not associated with posttrans-plant rejection, while IgG antibodies alone and mixture of IgG and IgM antibodies may mediate acute, or even hyperacute rejection.

Objective:To construct the Helicobacter pylori infected C57BL/6 mice model to observe the activation of NOD1/NF-κB signaling pathways in the gastric tissues,and study its roles in inflammatory response during Hp infection.Methods:6-8 week-old C57BL/6 mice were randomly divided into two groups,the Hp infection group and the control group,and mice were given by gavage every 48 h for five times with Hp or PBS,respectively.All the animals were sacrificed at different time point and the gastric tissue were stained with hematoxylin-eosin( HE);The mRNA expression of NOD1 and RIP2 in gastric tissues were examined by RT-PCR;Levels of IFN-βand IP-10 in mice serum were assessed by ELISA;Nuclear translocation of p65 in gastric tissue was detected by Western blot.Results:Hp infection elicits an inflammatory cell response,glands in gastric tissue were reduced or atrophic,as compared with that in the control group.The levels of IP-10 and IFN-βincreased in the model group, and peaked at 16 weeks after Hp infection.Hp infection increased the mRNA expression of NOD1 and the p65 content in nuclear between 24-120 h(P<0.05),and the highest level at 48 h,subsequently the expression levels were began to decrease.The mRNA expression level of RIP2 was up-regulated after Hp was administrated, peaked at 48 h and declined after 72 h.However, the expression levels would rise again at 120 h.Conclusion: Hp infection can activate the NOD1/NF-κB signaling pathways and induce the production of IFN-βand IP-10 in gastrics of mice.

Objective To research the relationship between quality of life among the elderly with chronic periodontitis (CP) and the severity of CP.Methods A total of 471 elderly patients seeking treatment in the affiliated Stomatology Hospital of Kunming Medical University from September 2013 to January 2014 and their companions were investigated using the questionnaire according to the Chinese version of the Oral health Impact Profile-14.Those who diagnosed with CP were separated into two groups according to Community periodontal index (CPI) through periodontal examination.Group A was diagnosed with the early CP,and Group B was diagnosed with the late CP.Database was established by Epidata and statistical analysis was performed by SPSS.Results A total of 237 elderly patients with early CP and 139 with late CP were recorded.The mean OHIP-14 scores of the former were 14.36 ± 6.67,and the score of the later were 15.65 ± 7.12 relatively.The two groups who lived through negative effects were compared.The results suggested in the 14 items of OHIP-14.The statistical significance existed in the following 4 items,obvious pain appeared,uncomfortable when eating anything,unsatisfied with own diet,become easy to lose one's temper to others (P＜0.05).Conclusion OHR-QOL of elderly patients with the late CP is lower than that of those with the early CP.

AIM:To investigate the effect of miRNA-181a inhibition on the proliferation, migration and cell cycle of the human multiple myeloma cell line RPMI 8226.METHODS:Real-time PCR was used to detect miRNA-181a expression in serum samples from multiple myeloma or healthy subjects .After transfection with miRNA-181a inhibitor, the cell viability was examined by CCK-8 assay and colony formation assay .The cell migration ability was analyzed by wound healing assay .The cell cycle was detected by flow cytometry .Moreover , the protein level of cyclin D 1 and the phosphoryla-tion of PI3K and Akt were determined by Western blot .RESULTS:The expression of miRNA-181a was significantly in-creased in the serum from multiple myeloma patients as compared with healthy group .Inhibition of miRNA-181a expression by transfection with miRNA-181a inhibitor remarkably decreased the cell viability , migratory ability, the population of G0/G1 phase and cyclin D1 protein expression in the RPMI8226 cells.However, the population of S phase and the phosphory-lation of PI3K and Akt were reduced .CONCLUSION:Down-regulation of miRNA-181a inhibits the viability and migra-tory ability in the RPMI8226 cells via inhibition of cell cycle and PI 3K/Akt signaling pathway .

Objective To evaluate the effect of propofol on first spike latency (FSL) of inferior colliculus neurons and explore the electrophysiological mechanisms underlying the propofol-induced loss of heating.Methods Forty-three SD rats of both sexes weighing 200-250 g were used in this study.FSL was recorded with glass recording micro-electrode inserted in inferior colliculus.Propofol 100 mg/kg was administered intrapefitoneally.FSL was recorded before and every t0 min after propofol administration when sound intensity was between 90 dB SPL and 10 dB SPL before threshold.First-order exponential function was used to fit FSL-sound intensity curve at different time points before and after propofol administration.Results The inferior colliculus neurons showed offset response in one rat.FSL extended to 0.8 ms at 10 min after propofol administration.In the remaining 42 rats,the inferior colliculus neurons responded only to the beginning part of the sound.FSL was prolonged at 10 min after propofol administration.R2 of first-order exponential function ＞ 0.95 at different time points after propofol administration ( P ＜ 0.05 ).FSL-sound intensity curve was shifted parallelly upwards after administration.Conclusion Propofol affects auditory information transmission by extending FSL of rat inferior colliculus neurons but does not change the meaning of the information encoded by FSL.

Adult ; Aluminum Silicates ; toxicity ; Humans ; Middle Aged ; Mining ; Occupational Exposure ; Prevalence ; Silicosis ; epidemiology

Crohn Disease ; pathology ; Humans ; Infant ; Male

Transient receptor potential vanilloid 1 (TRPV1) is a non-selective positive ion channel that is mainly expressed in sensory neurons and a member of transient receptor potential (TRP) family. The receptor could be activated by mechanical irritation, chemical irritation or endogenous ligand to mediate pains and cause injury to body functions. Traditional Chinese medicine believes that the mechanism of pain is that "stagnation leads to pain". Specifically, both of the contracture and tautness caused by cold and the blood stasis could result in blood impassability and pain. Most of traditional Chinese medicines for clearing heat and removing toxicity have the anti-inflammatory effect, while those for warming interior, and promoting blood circulation to remove blood stasis have the effect in smoothening blood vessels. Therefore, either with the anti-inflammatory effect or the effect in smoothening blood vessels, traditional Chinese medicines for clearing heat and removing toxicity, warming interior, and promoting blood circulation have the analgesic effect In this paper, the authors summarize the analgesic effect of the above three traditional Chinese medicines, with TRPV1 as the target.
Analgesics ; administration & dosage ; Animals ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Pain ; drug therapy ; genetics ; metabolism ; TRPV Cation Channels ; antagonists & inhibitors ; genetics ; metabolism

Bacterial Infections ; epidemiology ; microbiology ; China ; Drug Resistance, Bacterial ; Humans ; Microbial Sensitivity Tests