ObjectiveTo investigate the feasibility and importance of the application of simulation ward in medical practice between classes.MethodsTo divide the interns into two groups:the experimental group of 30 and the control group of 30. Both groups apply the simulation ward to practice between classes and both are assessed at the end of the practice.ResultsTo compare the final score of the experimental group and the control group,and significant differences are found in the following aspects:humanistic care,history taking,physical examination,medical record analysis,the theoretical knowledge examinations with the data of P ＜0.05.ConclusionThe application of simulation ward in medical practice between classes can improve the quality of clinical teaching.

Objective To investigate the correlation of uric acid(UA) level and the circadian rhythm of blood pressure in hypertensive patients. Methods Among the individuals who presented to the cardiology clinic, 70 patients who had hypertension and were diagnozed with non- dipper hypertension (non-dipper hypertension group) by 24 h ambulatory blood pressure monitoring (ABPM), 70 patients with dipper hypertension patients (dipper hypertension group), and 52 normotensive individuals (control group) were enrolled in this study. Peripheral venous blood samples were collected from all the patients in order to evaluate the hematological and biochemical parameters. All the assessed parameters were compared among three groups. Results The level of UA in non-dipper hypertension group was the highest, in dipper hypertension group was higher and in contrl group was the lowerst:(393.57 ± 53.52), (280.57 ± 41.64), (267.66 ± 59.38) μmol/L, and there were significant differences (P<0.01). Multivariate Logistic regression analysis revealed that the level of UA was an independent risk factor for non-dipper circadian rhythm of blood pressure (P = 0.003, OR = 2.26, 95% CI: 1.34- 3.89). Conclusions The higher level of UA may be a risk factor for non-dipper circadian rhythm of blood pressure in hypertension patients.

Objective To discuss the nursing for patients with articular cartilage injury of knee joint after arthroscopic and stem cells transplantation.Methods With autologous bone marrow stem cell as the parent,stem cells were transplanted to patients' knee joints after separation and differentiation in vitro,promoting the recovery and reconstruction of the cartilage.Nursing intervention was implemented to 16 patients from preoperative preparation until after the surgery,to enhance patients' sense of self-protection and encourage them to do functional exercise actively.Results The success rate of operation was 100％ for the 16 patients,whose function was improved obviously after 4 weeks without recurrence and exacerbation.Conclusions Arthroscopic and stem cells transplantation can effectively alleviate the symptoms of articular cartilage injury of knee joint,and the whole nursing intervention can promote patients' functional recovery as soon as possible.

In the past few decades, our understanding of platelets has made great progress. Platelets play an unexpected central role in cancer and greatly affect the behavior of cancer cells. At the same time, the physiology and phenotype of platelets are also affected by cancer cells. Therefore, platelet-based tumor targeted therapy strategies have attracted the attention of researchers, but the limitations of their application require more attention. In this paper, the strategies of platelet-based tumor targeted therapy are summarized, and the strategies of platelet mimicking nanocarrier delivery, platelet hitch riding, platelet membrane coating biomimetic and engineered platelet targeting are mainly introduced. The easy activation, hard storage and unknown functional and phenotypic changes of platelets were discussed. At the same time, the strategy of platelet-based targeted tumor therapy is reviewed from theoretical basis and practical application. The development potential of platelets in the field of tumor diagnosis and treatment is discussed, which will provide some theoretical reference for the study of platelet-related tumor diagnosis and targeted therapy.

Background The excitotoxicity to retinal neurons caused by abnormal elevation of glutamate in retina is a common pathology concomitant with major blind-causing eye diseases.However,an effective approach to protect retinal neurons from glutamate-induced excitotoxicity is still lack.Intraperitoneal administration of α-melanocyte stimulating hormone(α-MSH)has been shown to protect hippocampal neurons from glutamate-induced excitotoxicity.Objective This study was to investigate the protective effect of α-MSH on glutamate-induced excitotoxicity in a chicken embryonic retinal explant culture system.Methods The retinas were isolated from chick embryos at embryonic day 9(E9) and cultured as explants.The explants at 3,5 and 7 days in vitro and the retinas at corresponding embryonic day 12,14 and 16(E12,E14,E16)were collected.The morphology of explant cultures was examined by hematoxylin and eosin staining,and the expression of melanocortin receptors (MCRs)was analyzed by real-time PCR.In the experiment of glutamate-induced excitotoxicity,the retinal explants at 4 days in vitro were treated with glutamate for 48 hours,α-MSH was incubated with the explants 30 minutes before and during the glutamate treatment period.Then the apoptotic cells were detected by TUNEL staining and quantified.The glutamate alone treated-explants and those treated with culture media were included as controls.The expression of glial fibrillary acidic protein(GFAP) at 48 hours after treatment in all retinal explants was analyzed by real-time PCR.Results Hematoxylin and eosin staining showed that the retinal explants exhibited similar morphology to those observed in the retinas from chick embryos at the corresponding developmental stages.The real-time PCR analyses of chick retinas showed that MC1R mRNA level at E9,E12,E14 and E16 was significantly lower than that in post-hatch day 1 (all P=0.000) ;whereas the transcript level of MC5R was significantly increased from E9 to E12 and E14 (both P =0.000),and then gradually decreased from E14 to P1.The expression of these genes showed similar temporal patterns in the retinal explant cultures.TUNEL staining revealed that treatment of the retinal explant cultures with α-MSH substantially and significantly reduced number of apoptotic cells induced by glutamate (P =0.000),which was accompanied by significant suppression of glutamate-induced GFAP up-regulation (P =0.000).Conclusions Application of α-MSH dramatically ameliorated glutamate-induced cell death in retinal explant cultures.This protective effect may be due to α-MSH-mediated suppression of astrogliosis caused by abnormal elevation of glutamate.

Objective To investigate the effect of sperm -associated antigen 9 ( SPAG9 ) on migration and invasion abilities of human osteo-sarcoma U2OS cells.Methods siRNA was used to decrease SPAG9 expression, and then cells were subjected to CCK -8 assay, wound healing assay and transwell assay to detect siSPAG9 on cell migration and invasion abilities. Western blot and Gelatin zymography were used to detect matrix metalloproteinase-2 ( MMP-2) and matrix metalloprotei-nase-9 ( MMP -9 ) expression and activities. Results siRNA for SPAG9 significantly decreased SPAG9 mRNA and protein expression. siSPAG9 had no effect on the proliferation of U2OS cells, but significant-ly inhibited its wound healing, migration and invasion abilities. The activities and expression of MMP-2 amd MMP-9 were also suppressed by siRNA for SPAG9.Conclusion Depletion of SPAG9 results in the inhi-bition of migration and invasion abilities of human osteosarcoma U2OS cells.

Objective To explore the relationships among negative life events,meaning in life and suicidal risk of college students.Methods A total of 897 students from southern medical university were surveyed by adolescent life events scale (ALES),suicidal behaviors questionnaire-revised (SBQ-R) and meaningful life measure-Chinese revised (MLM-CR).Results The scores of negative life events,meaning in life and suicidal risk were 1.03±0.88,124.32±20.35 and 4.17± 1.66,respectively.There were 64 (7.1％) college students with high risk of suicide.The overall stress score was positively correlated with total suicidal risk score (r=0.134,P＜0.01).Total suicidal risk score was negatively correlated with total score of meaning in life(r=-0.340,P＜0.01).Moderating effects of meaning in life between negative life events and suicidal risk were significant (P＜0.01).Meaning in life fully mediated the effects of negative life events on suicidal risk (P＜0.01),which accounted for 59.6％ of the total effects.Conclusion Meaning in life as a mediator and moderator between negative life events and suicidal risk.Meaning in life might play protective role in reducing the suicidal risk.

Objective To revise and evaluation the psychometric properties and usability of the Personal Meaning Profile Brief( PMP-B) . Methods 1256 university students were measured by PMP-B, Purpose in Life ( PIL) and the Satisfaction with Life Scale ( SWLS) ,and 234 university students were meas-ured by Meaningful Life Measure ( MLM) .321 university students were measured by PMP-B twice at differ-ent time after four weeks to analyze the reliability and validity of PMP-B. Results The items discrimination indices were all above 0.4(r=0.470-0.833,P<0.01). The internal consistency reliability of PMP-B was 0.957,the split-half reliability was 0.936 and the test-retest reliability was 0.852. Confirmatory factor analysis confirmed the hypothesized seven-factor model of PMP-B (χ2/df=3.17,GFI=0.965,AGFI=0.947,RMR=0.042,NFI=0.977,CFI=0.982,RMSEA=0.042). The criterion correlations with PIL,MLM and SWLS were 0.710,0.842 and 0.756 respectively (P<0.01). The PMP-B total score of the university students was (112.07±20.02),and female had high life meaning than male which was more positive in interpersonal rela-tionship,life belief and self-acceptance. Conclusion The reliability and validity of PMP-B are favorable,it can assess people''''s life meaning from different sources.

This paper discussed the concept, categorizations, methods and basic principles for establishing of soft index survey tools (SIST) in clinical medicinal field, pointed out the relation between the connatural theoretical and practical mode of TCM and the establishment of SIST having TCM characteristics, and elaborated several key points which should be paid attention to in the establishment of SIST. It was pointed out that there was broad space for application of SIST in TCM study.
Diagnosis, Differential ; Health Status Indicators ; Humans ; Medicine, Chinese Traditional ; methods ; Quality of Life ; Research Design ; Surveys and Questionnaires

Non-randomized studies (NRS) have gradually attracted people's attention in the field of clinical medicine. In this paper, the importance of NRS was emphasized on the viewpoints of the coherence of scientific research, the limitation of randomized control trials (RCTs), the immanent characteristics of TCM clinical practice and the actual requirement of complex intervention in clinical practice. And two main points in evaluation and implementation of NRS differed to those in RCTs were put forward, i.e. the first, the full-scale design of the study with the professional characteristics should be described very explicitly, especially the speciality of the objects, intervention elements and indexes for effectiveness evaluation, etc.; the second, the control of bias and evaluation of effectiveness, and their influence on conclusion induction should be fully taken into consideration. At the same time, the key step in improving the quality of NRS of TCM and integrative medicine was preliminary discussed in this paper.
Clinical Trials as Topic ; Humans ; Medicine, Chinese Traditional ; Randomized Controlled Trials as Topic ; Research Design