2.A STUDY ON BIOTECHNOLOGY OF GLYCOLIPID PRODUCED BY A BACILLUS COAGULANS
Xi-Qun ZHENG ; Xiao-Lan LIU ; Lu ZHANG ; Wei MA ;
Microbiology 1992;0(04):-
The biotechnology of glycolipid fermented b y a bacillus coagulans was studied and the fermentation pro cess in 10L bioreactor was conducted.Suitable medium contained 6% bean oil as ca rbon source,3 5g/L NaNO 3 as nitrogen source,0 75g/L yeast extract and some i no rganic salts.The fermentation temperature of 30℃,initial pH of 8 5,strring rev olution of 150~240r/min and fermentation period of 96h proved to be optimal.The yield of glycolipid in 10L bioreactor was 7 073g/L.
3.Aggressive systemic mastocytosis: report of a case.
Mi WANG ; Qun-Pei YANG ; Xiao-Qing WANG ; Xia XU ; Wei-Ping LIU
Chinese Journal of Pathology 2010;39(11):775-777
Antineoplastic Agents
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therapeutic use
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Diagnosis, Differential
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Female
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Histiocytosis, Langerhans-Cell
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metabolism
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pathology
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Humans
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Interferons
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therapeutic use
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Leukemia, Mast-Cell
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metabolism
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pathology
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Mastocytosis, Cutaneous
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metabolism
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pathology
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Mastocytosis, Systemic
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diagnostic imaging
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drug therapy
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metabolism
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pathology
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Middle Aged
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Proto-Oncogene Proteins c-kit
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metabolism
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Radiography
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Radionuclide Imaging
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Spleen
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pathology
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surgery
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Splenectomy
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Tryptases
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metabolism
6.T2 mapping Imaging of Lumbar Intervertebral Disc Degeneration by Using 1.5T Magnetic Resonance
Qun WEN ; Fanhua MENG ; Ting QIAN ; Chunxiao WEI ; Jun LIU ; Jing XIAO
Chinese Journal of Medical Imaging 2017;25(7):531-535
Purpose To investigate the value of MRI T2 mapping imaging in diagnosis of lumbar intervertebral disc degenerative (IVDD).Materials and Methods A prospective study was performed on 100 patients who underwent 1.5T MRI examination because of lumbar and back pain from October 2013 to December 2015 at the Fifth People's Hospital of Shanghai,Fudan University.The MRI examinations included conventional sagittal T1WI and T2WI,axial T2WI and median sagittal T2 mapping imaging.According to Pfirrmann standard,475 lumbar intervertebral discs were graded.T2 values of nucleus pulposus (NP) and annulus fibrosus (AF) of anteriority and posterior with different grades were measured and compared.The correlations between T2 value,age and grade were further analyzed.30 patients were selected and underwent a second MR examination after half a year,and the difference of T2 values between the two MR examinations were compared.Results Except for grade Ⅳ and grade Ⅴ,the differences of T2 values of NP between the other grades of lumbar intervertebral discs were statistically significant (P<0.05).T2 values of NP were negatively correlated with the grades (r=-0.77).There were no significant differences in T2 values of NP and AF of anteriority and posterior in 30 patients with back pain between the two MR measurements of half year interval (P>0.05).Conclusion T2 mapping imaging can quantitatively assess the degree of IVDD,especially T2 values of NP can reflect the differences of IVDD with grade Ⅰ-Ⅳ,so it can provide the imaging evidence for the diagnosis of IVDD.
7.MSCT measurement of anterior clinoid process and clinoid segment of internal carotid artery
Jiangjun QIN ; Hongxiu XIAO ; Rong TU ; Xiaolu ZHOU ; Qun QIN ; Wei TANG
Journal of Practical Radiology 2014;(5):740-743
Objective To observe the anatomy status of the anterior clinoid process (ACP)and the anterior clinoid segment of in-ternal carotid artery (ICA)respectively by multisliced computed tomography (MSCT),and to provide useful imaging information for ACP removal surgery.Methods A total of 100 patients (200 sides)had volume rendering reconstruction of skull.Cranium was removed along cranio-orbital bone in simulation.Then the anatomical structures of the ACP and its surrounding were observed in cephalad direction.The total length,medium length,basic width,medium width of the ACP and the sagittal view curve length of anterior clinoid segment of the ICA from both sides were measured.Results Total length of left ACP was (9.82±2.48)mm,basal width was (9.47±1.88)mm,medium length was (5.03±1.55)mm,medium width was (6.1 9 ±1.75)mm;for right side total length was (10.41±2.1 6)mm,basal width was (9.66 ±2.21)mm,medium length was (5.86 ±2.48)mm,medium width was (6.66±1.5 1)mm.Left anterior clinoid segment of ICA curve length was (6.74±2.25)mm;right was (8.54±3.00)mm.Paired sample t test showed no significant difference in total length,basal width and medium width of ACP in both sides (P >0.05);while the difference in medium length and curve length of the anterior clinoid segment of ICA were statistically significant respectively (P <0.05).Conclusion MSCT can clearly display the vivisection and variation status of the ACP and the anterior clinoid segment of the ICA and can provide useful imaging information for removal of ACP in operation.
8.Multislice Spiral CT-based Simulation in Enlarging Exposure Extent of Internal Carotid Artery Siphon Before and After Removing Anterior Clinoid
Jiangjun QIN ; Hongxiu XIAO ; Rong TU ; Xiaolu ZHOU ; Qun QIN ; Wei TANG
Chinese Journal of Medical Imaging 2013;(11):801-803
Purpose To study the exposure extent of internal carotid artery siphon (ICAS) before and after removing anterior clinoid process (ACP) using multislice spiral CT (MSCT) simulation, and to improve the tumor resection rate and ensure the operation effect. Materials and Methods MSCT three-dimensional images reconstruction simulating supraorbital keyhole approach of 100 patients (200 sides) were observed, the distance between the crotch of anterior cerebral artery and middle cerebral artery and ICAS before and after removing ACP (exposure extent) was measured. Results In 100 patients (200 sides ACP), the exposure extent before and after removing ACP were (14.3±3.9) mm and (30.5±4.2) mm, respectively on the left side with statistical difference (t=45.278, P<0.001), and were (15.9±3.8) mm and (31.8±3.9) mm, respectively on the right side with statistical difference (t=40.513, P<0.001). The exposure extent increased (16.3±3.6) mm and (15.8±3.9) mm, respectively on the left and right side with no statistical difference (t=0.251, P>0.05). Conclusion MSCT simulating supraorbital keyhole approach in removing ACP can effectively increase the exposure length of ICA, and enlarge the exposure extent of sella region, thus provide reliable imaging information for removing tumor and selecting surgical project in this region.
9.Distribution of CD68+ Monocytes/Macrophages in Human Normal Dermis
Yingjun GUO ; Yuming ZHAO ; Yakun WANG ; Yi XIAO ; Qun WEI ; Hongduo CHEN
Chinese Journal of Dermatology 1994;0(02):-
Objective To study the distribution, morphology and density of CD68+ monocytes/macro,phages in normal human dermis. Methods Normal skin specimens from 6 sites including face, trunk, proximal limbs, distal limbs, palms and soles of 8 adults were collected. The epidermal sheets, horizontal and longitudinal sections of the specimens were stained with an ABC immunoperoxidase procedure with anti-CD68 monoclonal antibodies. Results The CD68+ cells were detected within the superficial dermis with various densities: 562 ? 230/mm2 at distal limbs, 517 ? 162/mm2 at trunk, 509 ? 235/mm2 at face, 507 ? 192/mm2 at palms, 472 ? 138/mm2 at proximal limbs and 361 ? 78/mm2 at soles. Lower densities of CD68+ cells with dendritic morphology were found in the deep (reticular) dermis, and dispersed among collagen bundles. Conclusions There are CD68+ monocytes and macrophages in the superficial dermis, forming a relatively dense network of defense. Such a distribution might indicate the clear polarity of the CD68+ monocytes/macrophages in the dermis, with their direction of defense towards the dermal-epidermal junction.
10.Determination of Paeonol in Yuxianling Granules by HPLC
Qun HE ; Pingan LIU ; Biqing ZHAO ; Wanzhong LI ; Wei GONG ; Xiao LIANG
China Pharmacy 2005;0(15):-
OBJECTIVE: To establish a HPLC method for the determination of Paeonol in Yuxianling granules. METHODS: The determination was performed on Phenomenex C18-ODS with mobile phase consisted of methanol-water (60∶40) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 274nm. RESULTS: The linear range of Paeonol was 8.24~41.20?g?mL-1(r=0.999 7). The recovery was 99.50% (RSD=1.12%). CONCLUSION: The established method is simple, rapid, sensitive, accurate, stable and reproducible, and suitable for the determination of the content of paeonol in Yuxianling granules.