China ; Humans ; Liver ; surgery ; Translational Medical Research

p16 tumor suppressor gene plays an important role in early diagnosis for lung cancer. The occurrence of lung cancer is closed associated with p16 gene aberrant methylation in promotor, seconed exon homozygous deletion, gene point mutation , lose of protein and mRNA expression. Also p16 is related to gene therapy and drug target therapy. This review will summarize the relationship between p16 and lung cancer.

Laparoscopic hepatectomy has many advantages over open surgery, including minimization of local injury, reduced systemic reactions, and faster postoperative recovery. The aim of this "Consensus" is to provide guidance and reference to surgeons who perform, or are interested in performing laparoscopic liver surgeries.

Hepatectomy ; history ; methods ; History, 20th Century ; History, 21st Century ; Humans

Humans ; Hypertension, Portal ; surgery ; Liver ; pathology ; surgery ; Liver Failure ; surgery ; Liver Neoplasms ; surgery ; Liver Transplantation

Bile Duct Neoplasms ; pathology ; surgery ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma ; pathology ; surgery ; Hepatectomy ; methods ; Hepatic Artery ; pathology ; surgery ; Humans ; Lymph Node Excision ; methods ; Portal Vein ; pathology ; surgery

Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Central Nervous System ; drug effects ; growth & development ; Female ; Organophosphorus Compounds ; toxicity ; Pesticides ; toxicity ; Pregnancy ; Rats

Anionic surfactants in waters were determined using on-line double microporous membrane extraction-flow injection analysis method. This method was based on the extraction of the ion-association complex (λ_(max)=650 nm) which formed from methylene blue cation and anionic surfactants. The on-line double microporous membrane liquid-liquid extraction phase separators were adopted. Optimization of variables of the flow injection manifold, groove mechanical dimension of the phase separator, and pore size of the membrane was performed. The proposed method had a linear range of 25.0-1000.0 μg/L(r≥0.999). The detection limit was 4.28 μg/L. The relative standard deviation(n=7) of different concentration was 0.7%-6.0%. The recovery was between 96%-110% and the sample throughput was 18 h-1. The method was applied to the analysis of standard Reference materials with satisfactory results. For practical application, two water samples were analyzed with the established method.

Objective To compare the efficacy of vidarabine monophosphate and ribavirin for treating hand-foot-mouth disease (HFMD) in children.Methods 120 children with HFMD and admitted to a hospital from April 2013 to April 2015 were randomly divided into observation group and control group,60 cases in each group,observation group treated with vidarabine monophosphate,control group treated with ribavirin,the therapeutic efficacy between two groups were compared.Results After treatment,the negative conversion rate of intestinal virus EV and EV71,as well as Coxsackie virus A16(CA16) in observation group were 87.0％,85.7％,and 93.1％ respectively,in control group were 64.0％,69.8％,and 78.6％ respectively,difference between two groups was statistically significant (all P＜0.05).The average length of hospital stay in observation group was shorter than control group ([4.5 ± 1.3] days vs [6.2 ± 1.2] days,P＜0).05),symptom(fever,stomatitis,skin rash) control time in observation group were all shorter than control group(all P＜0.05).Whether patients with or without complication,therapeutic effect of the observation group was better than that of control group;myocardial enzyme data,C reactive protein (CRP),and white blood cell(WBC) in observation group were all lower than control group(all P＜0.05).Conclusion Efficacy of vidarabine monophosphate for treating children with HFMD is better than ribavirin,but it needs further study.

Objective To evaluate the effect of activated killer cell immunoglobulin-like receptor (S-KIR) on acute rejection (AR) after cadaveric liver transplantation. Methods Fifty-six randomized pairs of donors and recipients of liver transplantation were investigated to define their KIR genotype with PCR-SSP method. The perioperative clinical data of recipients were collected and statistically analyzed by SPSS 16.0. Results Inhibitory KIR (L-KIR, genotype AA) accounted for 50% (28/56) in the liver transplantation patients, of whom the expression frequency was 0.499; S-KIR (genotype AB/BB) accounted for 50% (28/56), of whom the expression frequency was 0.500. Out of the 56 pairs, AR occurred in16 recipients with an incidence of 28.6%. AR occurred 7-30 days after operation, and the median time was 14 days. AR occurred in 18.8% (3/16) of the recipients with AA assortment and in 43.8% (7/16) with AB/BB assortment, and there was no significant difference between two groups (P>0.05). AR occurred in 25.0% (4/16) of the donors with AA assortment and in 12.5% (2/16) with AB/BB assortment, and there was also no significant difference between two groups (P>0.05). AR occurred in 16.67% of the recipients who expressed one activating KIR gene (S1), and in 83.33% of the recipients who expressed two or more than two activating KIR genes (S2), with significant difference between two groups (P<0.05). Conclusions The genotype assortment and number of S-KIR may be related to the AR occurrence after liver transplantation. KIR tests of recipients may have significant clinical value in research of the effect of low-dose, low concentration, and combined therapy with anti-rejection drugs after liver transplantation.