1.Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice.
Yan-yan WANG ; Ru-zhui CHEN ; Xiao-nani ZHU ; Jing LIU ; Zhi-hui LI ; Xiu-juan LIU ; Zhi-hui LI ; Xin NA ; Shan-shan LIANG ; Guo-guang QIU ; Wei ZHANG ; Hai WANG ; Xue-lan WANG
Chinese Journal of Applied Physiology 2012;28(3):221-224
OBJECTIVETo establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.
METHODSFive transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.
RESULTSNinety five homozygous transgenic mice were selected, and the results indicated that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.
CONCLUSIONExogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast, reliable and economical way to screen homozygous and hemizygous transgenic mice.
Animals ; Female ; Homozygote ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microinjections ; Mutation ; tau Proteins ; genetics
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