1.Analysis on medication rules of state medical master Yan Zhenghua from prescriptions with citri reticulatae pericarpium based on data mining.
Jia-Rui WU ; Wei-Xian GUO ; Bing ZHANG ; Xiao-Meng ZHANG ; Bing YANG ; Xiao-Guang SHENG
China Journal of Chinese Materia Medica 2014;39(4):618-622
The prescriptions containing pericarpium citri reticulatae that built by Professor. Yan were collected to build a database based on traditional Chinese medicine (TCM) inheritance assist system. After analyzed by data mining, such as apriori algorithm, the frequency of single medicine, the frequency of drug combination, the association rules between drugs and core drug combinations can be get from the database. Through the analysis of 1 027 prescriptions with pericarpium citri reticulatae, these prescriptions were commonly used to treat stomach aches, cough and other syndromes. The most frequency drug combinations were "Citri Reticulatae Pericarpium-Poria", "Paeoniae Radix Rubra-Citri Reticulatae Pericarpium" and so on. The drug association rules that the confidence was 1 were "Glycyrrhizae Radix ex Rhizoma --> Citri Reticulatae Pericarpium", "Paeoniae Alba Radix-Cyperi Rhizoma --> Citri Reticulatae Pericarpium", "Poria --> Citri Reticulatae Pericarpium", and so on. The drugs in the prescriptions containing pericarpium citri reticulatae that built by Professor Yan mostly had the effects of regulating the flow of Qi and invigorate blood circulation, which reflected the clearly thought when making prescriptions.
Citrus
;
chemistry
;
Data Mining
;
Drug Interactions
;
Drug Prescriptions
;
Drug Therapy, Combination
;
Drugs, Chinese Herbal
;
pharmacology
;
Humans
;
Practice Patterns, Physicians'
2.Study on chemical constituents of Achillea alpina.
Xiao-qing CHEN ; Meng WANG ; Xin ZHANG ; Wei-wei GUO ; Xia WU
China Journal of Chinese Materia Medica 2015;40(7):1330-1333
Twelve compounds were isolated from the aerial parts of Achillea alpina by column chromatographies on silica gel, Sephadex LH-20, and semi-preparative HPLC. The structures were elucidated on the basis of spectral analysis. The compounds were identified as pellitorine(1), 8,9-dehydropellitorine(2), (E,E)-2,4-undecadien-8, 10-diynoic acid isobutylamide(3), (E,E)-2,4-tetradecadien-8,10-diynoic acid isobutylamide(4),sintenin(5), 4',5,7,8-tetramethoxyflavone(6), chrysoplenetin(7), formononetin(8), aurantiamide(9), asperglaucide(10), artemetin(11), and eupatorin(12). compounds 1-5 were isolated from this plant for the first time, and compounds 6-10 were isolated from the genus Achillea for the first time.
Achillea
;
chemistry
;
Chromatography, High Pressure Liquid
;
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
;
Molecular Structure
;
Spectrometry, Mass, Electrospray Ionization
3.Analysis on principle of treatment of phlegm retention syndrome of Ma Peizhi based on apriori and clustering algorithm.
Jia-Rui WU ; Wei-Xian GUO ; Xiao-Meng ZHANG ; Xiu-Qin HUANG ; Bing YANG
China Journal of Chinese Materia Medica 2014;39(4):631-633
Analyzed the prescriptions for phlegm retention syndrome that built by Ma Peizhi by the association rules and clustering algorithm, the frequency of drug usage and the relationship between drugs could be get. And from that we could conclude the experiences for phlegm retention syndrome of Ma Peizhi of menghe medical genre. The results of the analysis were that 18 core combinations were dig out, such as Citri Exocarpium Rubrum-Eriobotryae Folium-Citri Reticulatae Pericarpium. And there were 9 new prescriptions were found out such as Aurantii Fructus-Citri Exocarpium Rubium-Eriobotryae Folium-Citri Reticulatae Pericarpium. The results of the analysis were proved that Ma Peizhi of Menghe Medical Genre was good at curing phlegm retention syndrome by using the traditional Chinese medicine of mild and light, such as the medicines of mild tonification, and clearing damp and promoting diuresis.
Algorithms
;
Data Mining
;
Databases, Factual
;
Drug Prescriptions
;
standards
;
Drugs, Chinese Herbal
;
therapeutic use
;
Humans
;
Mucus
;
chemistry
;
Practice Patterns, Physicians'
;
Respiratory Tract Diseases
;
drug therapy
4.Analysis on medication rule in prescriptions for hemoptysis of Menghe physician Ma Peizhi based on knowledge-discovery in database.
Jia-Rui WU ; Wei-Xian GUO ; Xiu-Qin HUANG ; Bing ZHANG ; Xiao-Meng ZHANG
China Journal of Chinese Materia Medica 2014;39(4):627-630
The clinical prescriptions of hemoptysis that built by Menghe physician Ma Peizhi were collected, and were analyzed by the method of unsupervised data mining, such as association rules and clustering algorithm. From the prescriptions, we got the frequency of drugs, the association rules among drugs, 10 core drug combinations and 5 new prescriptions. Accordingly, after the analysis, Menghe physician Ma Peizhi had rich experience in the treatment of hemoptysis. His therapy for hemoptysis was clearing heat and moistening lung, dispel heat from blood to stop bleeding, and enriching yin and blood. And we can also make a conclusion that the TCM inheritance support system is of great practical value for minning the old doctors' clinical experiences.
Data Mining
;
Databases, Factual
;
Drug Prescriptions
;
standards
;
Drugs, Chinese Herbal
;
chemistry
;
therapeutic use
;
Hemoptysis
;
drug therapy
;
Humans
;
Practice Patterns, Physicians'
5.Analysis on principle of treatment of cough of yan zhenghua based on apriori and clustering algorithm.
Jia-Rui WU ; Wei-Xian GUO ; Xiao-Meng ZHANG ; Bing YANG ; Bing ZHANG
China Journal of Chinese Materia Medica 2014;39(4):623-626
Based on the data mining methods of association rules and clustering algorithm, the 188 prescriptions for cough that built by Yan Zhenghua were collected and analyzed to get the frequency of drug usage and the relationship between drugs. From which we could conclude the experiences of Yan Zhenghua for the treatment of cough. The results of the analysis were that 20 core combinations were dig out, such as Bambusae Caulis in Taenias-Almond-Sactmarsh Aster. And there were 10 new prescriptions were found out, such as Sactmarsh Aster-Scutellariae Radix-Album Viscum-Bambusae Caulis in Taenian-Eriobotryae Folium. The results of the analysis were proved that Yan Zhenghua was good at curing cough by using the traditional Chinese medicine that can dispel wind and heat from the body, and remove heat from the lung to relieve cough.
Algorithms
;
Cough
;
drug therapy
;
Data Mining
;
Drug Prescriptions
;
Drug Therapy, Combination
;
Drugs, Chinese Herbal
;
therapeutic use
;
Humans
;
Practice Patterns, Physicians'
6.Comment on applications of data mining used in studies of heritage of experiences of national medical masters.
Jia-Rui WU ; Shi-Huan TANG ; Wei-Xian GUO ; Xiao-Meng ZHANG ; Bing ZHANG
China Journal of Chinese Materia Medica 2014;39(4):614-617
Data mining, as known as knowledge discovery in databases, is a non-trivial process of revealing the implied, previously unknown and potentially useful information from the massive data. In recently years, the applications of data mining in the field of pharmaceutical research of traditional Chinese medicine have widespread. Especially in the field of the heritage of experiences of na-tional medical masters, data mining plays an important role. In this study, we would expound of the use of methods of data mining in the heritage of experiences of national medical masters, and analyze their advantages and disadvantages, such as association rules, Bayesian networks, neural networks, and decision trees.
Data Mining
;
Databases, Pharmaceutical
;
Medicine, Chinese Traditional
;
Neural Networks (Computer)
7.Study on medication regularity of grand master of traditional Chinese medicine YAN Zheng-hua's Ostreae Concha-containing prescriptions based on data mining.
Jia-Rui WU ; Wei-Xian GUO ; Xiao-Meng ZHANG ; Bing YANG ; Bing ZHANG
China Journal of Chinese Materia Medica 2014;39(14):2762-2766
In this study, prescriptions were collected to establish a database based on the traditional Chinese medicine inheritance support system (TCMISS). Such data-mining methods as software's statistical statement module, data analysis module and apriori algorithm were used to analyze the frequency of single drug, the frequency of drug combination, the association rules and the core drug combinations of ostreae concha-containing prescriptions. The results showed that Ostreae Concha-containing prescriptions were frequently used to treat insomnia, vertigo, stomach-ache and other syndromes. The frequently used drugs included Ossis Mastodi Fossilia, Parched Ziziphi Spinosae Semen, Polygoni Multiflori Caulis, Salviae Miltiorrhizae Radix and Paeoniae Rubra Radix. The frequently used drug combinations included "Ostreae Concha and Ossis Mastodi Fossilia", "Ostreae Concha and Parched Ziziphi Spinosae Semen", and "Ostreae Concha and Polygoni Multiflori Caulis". The drug association rules with the confidence coefficient of more than 0. 95 included "Ossis Mastodi Fossilia-->Ostreae Concha", "Cocos Poria-Ossis Mastodi Fossilia-Ostreae Concha", "Ossis Mastodi Fossilia-Parched Ziziphi Spinosae Semen-->Ostreae Concha", and "Salviae Miltiorrhizae Radix-Ossis Mastodi Fossilia--Ostreae Concha".
Animals
;
Chemistry, Pharmaceutical
;
Data Mining
;
Databases, Pharmaceutical
;
Drug Prescriptions
;
Humans
;
Medicine, Chinese Traditional
;
methods
;
Ostrea
;
chemistry
;
Physicians
;
Software
8.Experimental study on toxicity and changes of biochemical indicator in acute bromoxynil poisoning.
Huan LIANG ; Xiao LIU ; Zhong-qiu LU ; Qiao-meng QIU ; Guo-xin HU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(8):494-495
Acute Disease
;
Animals
;
Behavior, Animal
;
drug effects
;
Female
;
Male
;
Mice
;
Mice, Inbred ICR
;
Nitriles
;
poisoning
;
Rabbits
9.Preparation and evaluation of mouse model of house dust mite-induced asthma.
Wei GUO ; Meng-Rong LI ; Jian-Jun XIAO ; Min HUANG
Chinese Journal of Contemporary Pediatrics 2008;10(5):647-650
OBJECTIVETo prepare a mouse model of asthma by sensitizing and challenging with house dust mite allergen Derp and evaluate its reliability by measuring airway allergy inflammation and airway responsiveness.
METHODSTwelve C57BL/6J mice were randomly divided into two groups: control and asthma model. Mice of the asthma model group were sensitized by intraperitoneal injection of house dust mite allergen Derp on the first and tenth days of the experiment. From the 17th day, the mice were challenged by intranasal Derp, once every other day, seven times. The control group was treated with normal sodium instead of Derp. Twenty-four hours after the last challenge, airway responsiveness was evaluated. Bronchoalveolar lavage and histological examination of the lung were performed.
RESULTSAirway resistance increased and dynamic lung compliance decreased significantly in the asthma model group as compared to the control group (P<0.01). When airway resistance increased by 25% and dynamic lung compliance decreased by 15%, the required metacholine concentration in the asthma model group was significantly lower than that in the control group (P<0.01). In the bronchoalveolar lavage fluid of the asthma model group, the number of total cells, absolute number of eosinophils (EOS) and the percentage of EOS in the total cell were significantly higher than those in the control group (P<0.01). Pulmonary pathological scores in the asthma model group were significantly higher than those in the control group (P<0.01). The asthma model group showed ultrastructural changes of bronchial and pulmonary arterioles. Goblet cells, mastocyte granules, and increased mucus were observed in the lung tissues of the asthma model group.
CONCLUSIONSA mouse model of asthma was prepared by sensitizing and challenging with house dust mite allergen Derp, with the characteristics of airway allergy inflammation and airway hypersensitivity reaction.
Airway Resistance ; Animals ; Arterioles ; ultrastructure ; Asthma ; etiology ; pathology ; physiopathology ; Disease Models, Animal ; Eosinophils ; pathology ; Female ; Lung ; pathology ; ultrastructure ; Lung Compliance ; Mice ; Mice, Inbred C57BL ; Pyroglyphidae ; immunology
10.Methylation in Promoter Region of SLC6A2 Gene in Heart Failure Patients and Its Correlation with Qi Deficiency/Blood Stasis Syndrome.
Jie-wei LUO ; Xiao-rong MENG ; Fang-meng HUANG ; Dan HU ; Xiao YANG ; Xing-yu ZHENG ; Shi-chao WEI ; Miao-miao GUO ; Shi-ming WU ; Hong-yi CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1448-1454
OBJECTIVETo explore the methylation status in promoter region of norepinephrine transporter gene (NET, SLC6A2) in heart failure ( HF) patients and its correlation with qi deficiency/blood stasis syndrome (QDS/BSS).
METHODSThirty-six patients with heart failure (NYHA classification III to IV) were recruited in the study (as the heart failure group) and their scores of QDS/BSS were evaluated. Besides, a healthy elderly group (30 cases) and a healthy youth group (30 cases) were also set up. They were recruited from Physical Examination Center of Fujian Provincial Hospital. Pyrosequencing was applied to detect the methylation in promoter region of SLC6A2 gene, and the total methylation index (MTI) of CpG island was calculated. The correlation between the methylation status in promoter region of SLC6A2 and scores of QDS/BSS was assessed using Pearson and Partial analyses. Risk factors were screened and adjusted using Logistic regression.
RESULTSBy one-factor analysis of variance, the total MTI in the HF group (219.72% ± 54.03%) was obviously higher than that in the healthy elderly group (194.47% ± 34.92%) and the healthy youth group (161.60% ± 41.11%) (all P < 0.05). Meanwhile, the total MTI was higher in the healthy elderly group than in the healthy youth group (P < 0.01). By covariance analysis , after controlling age and BMI, the total MTI was higher in the HF group than in the healthy elderly group (P = 0.041), while it was higher in the healthy elderly group than in the healthy youth group (P = 0.016). Age was found to play an essential role in affecting MTI of SLC6A2 gene promoter region among the 3 groups (F = 16.447, P = 0.01). The total MTI was quite lower in the healthy youth group. Results of Partial correlation analysis showed MTI was positively correlated with scores of qi deficiency and blood stasis respectively (r = 0.494 and 0.419 respectively, both P < 0.05). Logistic regression analysis showed after adjusting confounding factors, the relative risk (OR value) of total MTI of SLC6A2 gene in promoter region was 1.038 (95% CI, 1.006 to 1.071, P = 0.020).
CONCLUSIONSAbnormally elevated methylation of the promoter region of SLC6A2 gene is one of risk factors for HF. In addition, the degree of methylation of the promoter region of SLC6A2 gene was positively correlated with the severity of QDS/BSS.
Adolescent ; Aged ; DNA Methylation ; Heart Failure ; genetics ; physiopathology ; Humans ; Logistic Models ; Medicine, Chinese Traditional ; Norepinephrine Plasma Membrane Transport Proteins ; genetics ; Promoter Regions, Genetic ; Qi