Adult ; Brucellosis ; Humans ; Male ; Middle Aged ; Occupational Diseases

Adult ; Female ; Glottis ; Humans ; Laryngeal Mucosa ; transplantation ; Laryngoscopy ; Laryngostenosis ; surgery ; Lasers, Gas ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Young Adult

Objective:To study the influence of morphine on the expression of nestin in the ependymal epithelia,central gray and hippocampal formations in mice.Methods:Twenty health mice were evenly randomized into control group and experiment group.Mice in the control group were injected with normal saline(0.1 ml daily)and those in the experimental group were injected with morphine (0.1 ml,1 mg daily).Thirty days later,the mice brain samples were harvested and made into paraffin sections.Immumohistochemical ABC technique was used to observe the expression of nestin under light microscope.The images were analyzed with the image analytical system.Results:In the control group,the ependymal epithelia,the central gray,the periventricular gray substances and the hippocampal formations had weak expression of the nestin,with a mean gray scale of 150.98?13.31;there were 5 kinds of nestin-positive cells:(1) the basal cells of ependymal epithelium,(2)cells distributed in the periventricular gray substance and the deep lamella of central gray, (3)cells distributed in the superficial lamella of central gray,the subiculum,the parahippocampal gyrus and the cortex inⅡ,Ⅲlayers of the entorhinal area,(4)cells frequently seen in the rectum of midbrain and the subiculum,and(5)cells distributed in the tectum of midhrain,the hippocampus,gyrus dentatus,parahippocampal gyrus and the cortex in V layer of the entorhinal area;the density of nestin in the subiculum and entorhinal area was(7.20?1.23)mm~2.In the experiment group,the ependymal epithelia,the central gray,the periventricular gray substances and the hippocampal formations had positive expression of the nestin,with the mean gray scale being 133.03?22.28;the density of the above-mentioned 5 kinds of cells increased;the density of nestin in the subiculum and entorhinal area was(10.50?1.43)mm~2.The mean value of gray scale and nestin-positive neurons were significantly different between the 2 groups (P

Aim To ascertain whether defibrase has the protective effect against reperfusion injury after cerebral ischemia.Methods 70 renovascular hypertensive rats(RHR) were randomly divided into defibrase group, control group and sham-operated group.Reversible middle cerebral artery occlusion(MCAO) models were produced by the modified. Longa's method,and reperfusion was begun 2 hours after occlusion.Rats in the defibrase group were given defibrase 10 U?kg-1 body weight via femonal intraveneous injection, and in the control group with the same amount of saline. The brain pieces were processed by TTC and HE staining and the infarct size,brain microvessels damage and secondary bleeding were compared between the two groups. Results The volume of infarction in the defibrase group was obviously smaller than in the control group, the damage of brain microvessels was less severe, and the bleeding lesions under optical microscope were less than in the control group. Conclusion Defibrase has protective effect against reperfusion injury post cerebral ischemia.

Objective To build and to apply the delayering and overall rationing system nursing mode in obstetrics with a view to provide patients with the full range and comprehensive good nursing care.Methods 2 450 patients received traditional nursing care in 2009 and 2900 patients received the delayering and overall rationing system nursing in 2010 were divided into the control group and experimental group,respectively.Reconstituting nursing practice circuit,reforming the mode of scheduling,reinforcing reliable safeguards system and establishing the model of assess performance integration were carried out in experimental group.The satisfaction rate of patients in the two groups were compared.Results The overall satisfaction of inpatients in the two group was 82.13％ and 87.26％,and the satisfaction rate in patients who had left hospital was 92.56％ and 98.82,the satisfaction of basic nursing was 84.21％ and 96.60％ and the satisfaction of health education was 90.15％ and 99.23％ all of the differences were significantly obvious( x2 was 10.32,5.96,8.85,7.81,respectively;P ＜ 0.05 ).Conclusions The application of delayering and overall rationing system nursing mode can improve the satisfaction rate of patients it is a new and efficacious road to apply excellent nursing care.

Aged ; Humans ; Kidney Neoplasms ; pathology ; Male ; Neoplasm Metastasis ; Paranasal Sinus Neoplasms ; secondary

Adult ; Aged ; Aged, 80 and over ; Breast Neoplasms ; complications ; Female ; Humans ; Infusions, Intralesional ; Lung Neoplasms ; complications ; Lymphoma ; complications ; Male ; Middle Aged ; Pericardial Effusion ; etiology ; therapy ; Recombinant Proteins ; administration & dosage ; therapeutic use ; Remission Induction ; Tumor Necrosis Factors ; administration & dosage ; therapeutic use

Adult ; Case-Control Studies ; Cell Line, Tumor ; Female ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; immunology ; metabolism ; pathology ; Neoplasm Staging ; T-Lymphocytes, Cytotoxic ; immunology

Objective To investigate the expression of cyclin kinase inhibitor P21~(WAF1) and P27~(KIP1)in children with acute leukemia and its clinical significance.Methods A total of 32 hospitalized children with acute leukemia(AL) were included in this study.Their bone marrow samples were collected before chemotherapy and individual patient was detected after complete remission(CR).The method of immunocytochemistry was used to estimate the expression of P21~(WAF1) and P27~(KIP1).Both positive percentage and intensity of the cells were counted.Results Findings showed that the positive ratios of P21~(WAF1) and P27~(KIP1) in total samples,ALL samples and AML samples were lower than the control group(P

To evaluate the automated segmentation algorithm for detection of intra - retinal layers to process images obtained from ultra- high resolution optical coherence tomography ( OCT ) . Graph theory and the shortest path search based on dynamic programming were applied to automatically segment the 8 intra - retinal layers. We experimentally verified the accuracy and reliability of the algorithm. The results showed that the intra-retinal layer boundaries between automated and manual segmentations matched well. The algorithm successfully segmented the intra- retinal layers in glaucoma, high myopia, and retinitis pigmentosa patients. The proposed automatic segmentation for intra-retinal layers provides a promising tool for quantitative analysis in clinical diagnosis and treatment.