Objective: To study the role of mRNA expression of inducible nitric oxide synthase (iNOS) in the occurrence and development of oral squamous cell carcinoma (OSCC) and the relationship between the expressions of iNOS mRNA and the clinical pathologic characteristics of OSCC. Methods: In situ hybridization assay was used to detect iNOS mRNA in 10 cases of normal oral mucosa, 12 cases of oral epithelial simple hyperplasia, 28 cases of oral epithelial atypicall hyperplasia, and 32 cases of OSCC. Results: The expressions of iNOS mRNA was negative in the normal oral mucosa. The expression of iNOS mRNA in both atypical hyperplasia and OSCC groups were significantly higher than that in simple hyperplasia group (P <0.001). Statistical analysis showed a significant correlation between iNOS mRNA expression and the grade of atypical hyperplasia (P <0.05). No significant difference of iNOS mRNA expression was found between atypical hyperplasia and OSCC groups (P > 0.05). The expression of iNOS mRNA in the group of well-, moderately- and poorly-differentiated squamous cell carcinoma was significantly different (P < 0.05). The expression of iNOS mRNA in stage III and IV OSCC tissues was significantly higher than those at stage I and II, and significantly higher in OSCC patients with neck lymph node metastasis than those without neck lymph node metastasis (P <0.05). Conclusion: iNOS mRNA may play an important role in OSCC development and progression. The expression of iNOS has a close relationship with the differentiation, TNM stage, and neck lymph node metastasis of OSCC. Detection of NOS may be useful in evaluation of patient's prognosis and guidance of OSCC treatment.

Anti-Bacterial Agents ; therapeutic use ; Bacterial Infections ; diagnosis ; therapy ; China ; Communicable Diseases ; diagnosis ; therapy ; Cross Infection ; prevention & control ; therapy ; Granulocyte Colony-Stimulating Factor ; therapeutic use ; Granulocyte-Macrophage Colony-Stimulating Factor ; therapeutic use ; Humans ; Immunoglobulins, Intravenous ; therapeutic use ; Infant, Newborn ; Sepsis ; diagnosis ; therapy

Administration, Inhalation ; Adrenergic beta-Agonists ; adverse effects ; Child ; Extremities ; Humans ; Male ; Paralysis ; chemically induced ; Terbutaline ; administration & dosage ; adverse effects

Animals ; Arrhythmias, Cardiac ; etiology ; therapy ; Captopril ; therapeutic use ; Disease Models, Animal ; Electric Stimulation Therapy ; methods ; Myocardial Ischemia ; physiopathology ; therapy ; Rabbits ; Ventricular Fibrillation

Diagnosis, Differential ; Diarrhea ; etiology ; Humans ; Infant ; Male ; Mediastinal Cyst ; complications ; diagnosis ; surgery ; Mediastinal Neoplasms ; complications ; diagnosis ; Respiratory Sounds ; etiology ; physiopathology ; Treatment Outcome ; X-Rays

Objective:To investigate the efficacy of sequential therapy of CD19 and CD22 chimeric antigen receptor T cells (CAR-T) for relapsed/refractory mediastinal B lymphoblastic lymphoma (B-LBL).Methods:One patient with relapsed/refractory mediastinal B-LBL treated with sequential therapy of CD19 and CD22 CAR-T who was admitted to Tongji Hospital of Huazhong University of Science and Technology Tongji Medical College in March 2017 was reported. At 1, 3, 6, 12 and 18 months after CAR-T therapy, the indicators of primary disease remission were monitored and the relevant literature was reviewed.Results:The patient relapsed and progressed after third-line chemotherapy, and then received sequential therapy of CD19 and CD22 CAR-T. In the course of cellular immunotherapy, the patient presented grade 1 cytokine release syndrome. After active treatment, the patient got stable condition and was discharged. The patient came to the hospital for regular review, and the mediastinal mass of the patient was dynamically followed up. After CAR-T therapy, the mediastinal mass of the patient was significantly reduced, and the patient was in continuous remission for 18 months.Conclusions:Sequential therapy of CD19 and CD22 CAR-T provides a new therapeutic approach for relapsed/refractory B-LBL. For patients with poor curative effect of conventional chemotherapy, CAR-T therapy should be actively performed as soon as possible to improve the remission rate and the long-term prognosis of patients.

Objective To establish a method of reverse hybridization to detect five subfamilies of low risk Human Papillomaviruses(HPV6,11,42,43 and 44)and eighteen subfamilies of high risk HPV (HPV16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83 and MM4)in one reaction.Methods Special probes for twenty-three HPV subfamilies were fixed on nylon membrane bars,biotin labeled general primers mediated polymerase chain reaction(GP-PCR)were applied in HPV DNA amplification.PCR amplified DNA fragments were reversely hybridized with special probes that were fixed on the membranes. All samples(136)detected by reverse hybridization method were paralleled with the methods of Hybridization Capture Ⅱ(HC-Ⅱ)and sequencing.Results Positive rate of the 136 samples detected by reverse hybridization was 41.9%,while HC-Ⅱ 42.6% and sequencing 40.4%.Reverse hybridization detection indicated coherence with the other two methods(Kappa 0.8644 and 0.9089,respectively).While sequencing was lab standard for DNA test,the sensitivity was 96.36%,specificity was 95.06%,accuracy was 95.59%.Conclusions Method of reverse hybridization is adaptable to 23 kinds of HPV subfamilies, which can confirm the exactly subfamilies of HPV infection.This method is adaptable in clinical detection of HPV,with high sensitivity,high specificity,simply and convenient operation and the results are easily to be read.

Objective To discuss the valuable influence of the total-body massage on the facilitation of the rehabilitation for the women in puerperium.Methods Both Chinese traditional medical total-body massage and the biological information theories and its methodology were employed in dealing with 50 cases of puerperants without contraindications,who had labored naturally 12 hours before and who had received cesarean sections 48 hours before. The results were compared with the control group in which conventional preventive health care for puerperants was adopted.Results Notable differences exist in the aspect of lochia,defecation,lactation and emotions between the two groups.Conclusion Significant benefits can be obtained from the massage to facilitate the rehabilitation of the women in puerperium while promoting their quality of lives and broadening the coverage of services of the preventive health care for the puerperants.

Objective To simulate the mechanical environment of cells in vivo and study cellular signal transduction mechanisms. Methods A device was developed which could provide high cell yield, control the time, strain magnitude, direction and frequency of stretch, and applied 10% cyclic strain to cell culture substrate with stretch frequency at 1Hz. Results After being stretched, morphology and cytoskeleton of cells were altered. The major axis of cells and the alignment of stress fibers were vertical to the orientation of cyclic stretch. Conclusion This device provides versatile options for the study on the cellular responses of mechanical loading.

Objective To explore the relation between lower extremity deep venous thrombosis and knee injury.Methods 100 knee injury patients were operated on in our department.The difference of incidence rates of DVT,the thrombus location and incidence rates of DVT before and after operation in the two groups was compared.There were no fatal pulmonary embolism occurring in either group.Results The incidence rate of DVT in tibial plateau fracture group was 52％,that in patellar fracture was 30％ (P ＜ 0.05).DVT incidence rate of popliteal,pretibial,posterior tibial vein thrombosis in group of tibial plateau fracture was higher than that in group of patellar fracture (P ＜ 0.05),while there was no significant difference in postoperative thrombosis between the two groups(P ＞ 0.05).Conclusions There is a high incidence rate of DVT in knee joint injury.Which is closely related to the position of fracture and limb immobilization.