Nasal polyps is a common disease in clinical, with nasal mucosal inflammatory hyperplasia. However, the molecular mechanism of it is largely unknown. Tumor suppressor genes, proliferation and apoptosis related genes and cytokines play an important role in the formation and pathological development of nasal polyps. The expression level of tumor suppressor related proteins and receptors is not constant, high level of these expression can stimulate nasal mucosal proliferation, while low or lacking expression may facilitate nasal polyps cells apoptosis. Both proliferation and apoptosis make a big difference in nasal polyps formation and development. This article concludes the relationships between new progressions in nasal polyps pathogenesis and tumor suppressor genes and proliferation apoptosis genes, which not only helps practitioners deeply and comprehensively understand nasall polyps pathogenesis but promotes explorations to effective therapeutics.
Apoptosis ; Apoptosis Regulatory Proteins ; genetics ; Cytokines ; Disease Progression ; Genes, Tumor Suppressor ; Humans ; Nasal Mucosa ; Nasal Polyps ; genetics

Objective To explore the effects of vascular endothelial growth factor (VEGF) and nitric oxide (NO) on coronary collateral blood flow (CCBF) in a rabbit model of physical ischemic training (PIT),and to provide a foundation for the study of the mechanism of collateral circulation formation in PIT.Methods Models of intermittent myocardial ischemia were established in 55 rabbits by installing a water balloon constrictor on the left ventricular coronary artery.The rabbits were then divided into a sham-operation group,a pure ischemia group,a PIT group,a PIT group with NO inhibition pretreatment,a PIT group with VEGF inhibition treatment,and a PIT group with both NO and VEGF inhibition pretreatment.Microspheres were injected at modeling and before sacrifice before and after ischemic stimulation.At the termination of the experiment the ischemic myocardial tissue of the left ventricular anterior wall was sampled,and then relative CCBF was measured using the microspheres.Results There was no significant difference among the 6 groups before training.After training prominent differences among the groups were observed,with the PIT group having the greatest increase in CCBF.CCBF in the pure ischemia group,the PIT group,and the PIT group with NO inhibition pretreatment were significantly higher than in the sham-operation group.Significant differences were also found between the PIT group and the three pretreatment groups.CCBF in the PIT group with NO pretreatment and VEGF inhibition was significantly different from that in the NO pretreatment group,while it was not found in the group with VEGF inhibition pretreatment.The CCBF in the group with VEGF inhibition pretreatment and the group with NO inhibition pretreatment was similar.Conclusions PIT can improve CCBF to the remote ischemic myocardium when combined with VEGF and NO pretreatment.Blocking VEGF or NO can reduce CCBF after training,and both VEGF and NO are involved in the formation of collateral circulation,with VEGF playing the major role.

Objective To investigate the application value of three-dimensional power Doppler ultrasonography(3D-PDU) for synovial disease in patients with rheumatoid arthritis(RA). Methods Forty four patients with RA were divided into active (23 cases) and non-active (21 cases) groups. 3D-PDU was used in the wrists to measure the volume of synovium(V) ,vascular index(VI) in synovium.and Doppler spectrum was used to measure synovial artery peak systolic blood flow velocity(PSV) , end diastolic velocity (EDV) and resistance index(RI). Results There were significant differences of the V [(1. 73 0. 73) cm3] and VI (9. 53 6. 11) in the group of active patients compared to the V [(1. 09 0. 76) cm3 , P = 0. 008] and VI [(3.86 4.99), P = 0.000] in the ones of inactive patients. No differences were found for PSV [(16. 8 6. 29) cm/s vs (13. 5 8. 54) cm/s, P = 0. 282] ,EDV [(5. 51 1.77) cm/s vs (5. 03 2. 76) cm/s, P = 0. 539] and RI (0. 66 0. 07 vs 0. 62 0. 08, P = 0. 095)in the comparison of two groups. Conclusions 3D-PDU in determining RA disease activity in patients with synovial medium has a higher application value, VI can be used as an effective indicator to determine the disease activity.

Objective To explore the side effects of pemetrexed in the maintenane therapy of nonsmall-cell lung cancer (NSCLC) at advanced stage and summarize the nursing measures. Methods Fifty-one NSCLC patients were treated maintenance therapy by pemetrexed. The side effects were observed and corresponding nursing measures were taken. Results The side effects included bone marrow depression of 33 cases, adverse reactions in gastrointestinal tract of 14 cases, and skin rashes of 4 cases, all at the degree of I-II. All of them were alleviated after active treatments. Conclusion Such nursing measures as close of side effects and careful nursing to the NSCLC patients treated with maintenance therapy with temetrexed are main measures for the success of treatment.

OBJECTIVETo investigate the Toxoplasma gondii (TOX) infection in males with sterility and the effect of the infection on the reproductive function of males.

METHODSEnzyme linked immunoabsorbent assay (ELISA) was used to detect TOX-CAg, TOX-IgG and TOX-IgM in the peripheral blood of male patients with sterility.

RESULTSAmong 100 cases of male sterility, 7 were TOX-IgG positive (7%), 16 TOX-IgM positive (16%) and 13 TOX-CAg positive (13%). Among 100 normal males, 7 were TOX-IgG positive (7%), 3 TOX-IgM positive (3%) and 1 TOX-CAg positive (1%).

CONCLUSIONTOX infection may affect the fertility of males and cause male sterility. For this reason, males should prevent themselves from TOX infection.

Adult ; Animals ; Antibodies, Protozoan ; blood ; Antigens, Protozoan ; blood ; China ; epidemiology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin G ; blood ; Immunoglobulin M ; blood ; Infertility, Male ; epidemiology ; parasitology ; Male ; Toxoplasma ; immunology ; Toxoplasmosis ; complications ; epidemiology

[Objective] To investigate the expression of MICA mRNA and protein in osteosarcoma and give a more comprehensive understanding to its immune evasion.[Methods] RT-PCR was used to analyze MICA mRNA in 11 osteosarcoma tissues and 5 osteosarcoma cell lines.MICA expression was examined in 66 paraffin-embedded osteosarcoma tissues and 6 paraffin-embedded normal bone tissues by immunohistochemistry,and MICA protein in 9 fresh osteosarcoma tissues was detected by Western blot too.MICA surface expression was estimated by flow cytometry.[Results] Nine of eleven (81.8%) osteosarcoma specimens and all of the five cell lines consistently expressed MICA mRNA.Up-regulation of MICA expression was found in osteosarcoma (34/66,51.6%),compared with normal bone tissues (0/6,0%).The cell lines Saos-2,MG63,and HOS showed positive surface MICA expression,while the U2OS and OS732 showed very limited expression.[Conclusion] MICA mRNA and protein predominantly expressed on osteosarcoma tissues and cell lines.

In order to explore reasonable artificial cultivation pattern of Thesium chinense, the biological characteristics and nutrients change in the process of winter dormancy of T. chinense was studied. The phenological period of T. chinense was observed by using fixed-point notation and the starch grains changes were determined dynamically by PAS-vanadium iron hematoxylin staixjing method. Soluble sugar and starch content were measured by anthrone-sulfuric acid method and amylase activity was determined by DN'S method. The results showed that the normal life cycle of T. chinense was two years. T. chinense was growing by seed in the first year, but growing by the root neck bud in the second year. During the process of dormancy, starch and soluble sugar could mutual transformation in different periods. T. chinense had sufficient carbohydrate to maintain growth and also a lot of small molecules to improve their ability to fight against adversity.
Plant Dormancy ; Plant Leaves ; chemistry ; growth & development ; metabolism ; Plant Roots ; chemistry ; growth & development ; metabolism ; Plant Stems ; chemistry ; growth & development ; metabolism ; Santalaceae ; chemistry ; growth & development ; metabolism ; Seasons ; Starch ; analysis ; metabolism

Objective To study the effect on the environmental radioactivity level and the health of the residents in Shanghai when Qinshan Nuclear Power Station(QNPS) has been in normal operational condition for 17 years. Methods The field monitoring and the sampling analytical method were used for monitoring the radioactivity level in outdoor environment, drinking water, food and soil. Results The outdoor natural radiation level and the radioactivity level in the sample of the environmental media were on the normal background level. There was no distinct change in the environmental radiation level of Jinshan Area when QNPS was in normal operational condition. The radioactivity level in the environmental media was far lower than the limit values specified by the national standard GB. The effective dose of the outdoor natural external exposure for the residents is about 0.102 mSv/a and the effective dose of the internal exposure from the artificial radionuclides 90Sr and 137Cs in food for the residents is about 0.488 μSv/a. Conclusions There is no effect of the radioactive contamination on the environmental radioactivity level and the health of the residents in Shanghai when QNPS has been in normal operational condition for 17 years.

Response Surface Methodology was applied to optimize the culture components for lipopeptide production by Bacillus natto TK-1. In the first step, two level factorial design of Plackett-Burman was used to evaluate the influence of six related factors. It showed that three factors playing the important roles in the medium, including peptone, yeast extract powder and CaCl_2. The path of steepest ascent was used to approach the optimal region of the fermentation conditions subsequently. In the third step, the concentrations of those three main factors were further optimized by using Box-Behnken and Response Surface Analysis. By solving the quadratic regression model equation, the optimal concentrations of the variables were determined as: peptone 1.73%, yeast extract powder 0.063 %, CaCl_2 1.385?10-4mol/L. Under the optimal culture conditions, the diameter of haemolysis zone increased 29.3 % than before. HPLC analysis showed the precise production of lipopeptide was 30.2% higher than preliminary culture. Furthermore, at three batches cultivation, the experiment values under the optimal conditions agreed with the predictive values. It showed that Response Surface Methodology was proper and a good choice for optimization.

Fermentation for Eicosapentaenoic Acid(EPA) production by Nitzschia laevis at various temperature between 10℃ and 30℃ was investigated and the dynamics characteristics during fermentation process were also analyzed.Based on the results,a varying temperature nursing method of two stage control strategy is proposed:During the first stage,which comprises the delay phase and the initial index phase,the temperature is maintained at 25℃;then the temperature is shifted to 20℃ and kept up till the end of the fermentation process.By this method,a EPA content of 6.0% and a yield of 291.60 mg/L have been gained.These are 24.07% and 18.81% higher than that of fixed temperature(25℃) fermentation,respectively.