Animals ; Male ; Prostate-Specific Antigen ; analysis ; metabolism ; Prostatectomy ; adverse effects ; standards ; Specimen Handling ; methods ; standards

The tumor microenvironment is closely related to the occurrence and development of tumor. Hypoxia is considered to be one of the most important factors in tumor microenvironment.Formation of hypoxic microenvironment can be found in most of malignant tumors,which can inhibit the anti-tumor immune response. Recent studies have indicated that immunosuppressive cells,tumor stem cells and circulating tumor cells in hypoxic tumor microenvironment can mediate immune suppression and immune tolerance,and then promote development of tumor.The new immune therapy will focus on normalizing tumor vasculature,reconstructing the tumor microenvironment,avoiding immune suppression and averting tumor immune tolerance.

OBJECTIVE:To discuss the curative effects,adverse drug reactions(ADRs)and cost—effectiveness of 3 kinds of nitroimidazole drugs for treatment of trichomonal vaginitis.METHODS:111 cases of trichomonal vaginitis were randomly divided into 3 groups,administered with metronidazol(group A),tinidazole (group B),and ornidazol(group C)respectively,the curative effects and ADRs were observed and cost-effectiveness analysis was made in all groups.RESULTS:The costs in 3 groups were 0.74 yuan,33.6 yuan and 41.2 yuan respectively.The cure rates were 59.4%,81.6%and 97.2%,respectively.The cost-effectiveness ratios were 0.01,0.41,and 0.42 respectively.The increment cost -effectiveness ratios in group B and C were 1.48 and 1.07,as compared with group A.The occurrences of ADRs were 62.2%,18.4%,and11.1%respectively.CONCLUSION:Among 3 regimes,regime C is the preferred one.

Objective To study the high polar constituents of Gastrodia elata.Methods The constituents were isolated by macroporous resin column chromatography combined with preparative HPLC and identified by spectral analyses and physico-chemical properties comparisons.Results Ten compounds were isolated from H2O and 20% EtOH fractions eluted from resin column and their structures were identified as citric acid monoethyl ester(Ⅰ),uridine(Ⅱ),adenine(Ⅲ),dactylose A(Ⅳ),uracil(Ⅴ),tyrosine(Ⅵ),N2-(p-hydroxybenzyl) guanosine(Ⅶ),1-isoferuloyl-?-D-glucopyranoside(Ⅷ),4-(?-D-glucopyranosyloxy) benzaldehyde(Ⅸ),and p-hydroxyl benzoic acid(Ⅹ).Conclusion Compound Ⅶ is a new compound named gastronucleoside.Other nine compounds are isolated from G.elata for the first time.

Objective To investigate the effectiveness of interventional therapy for the atherosclerotic renal artery occlusion (ARAO). Methods During the period of June 2001-Dec. 2007, 16 patients with ARAO (total of 16 occluded arteries) underwent interventional managements, including percutaneous endovascular renal artery revascularization, balloon dilatation angioplasty and stent placement. Follow-up survey was made at regular intervals. The patent condition of the renal artery was evaluated with ultrasonography and digital subtraction angiography. The blood pressure and the renal function were determined and the data were statistically analyzed in order to assess the intermediate and long-term effect of the interventional therapy. Results Of 16 patients, technical success was achieved in 15 (93.8%) and failure occurred in one. During a follow-up period of 9 - 24 months, 3 patients died. According to the data obtained at each patient’s last follow-up survey, the hypertension fell to normal in 3 (25.0%), was improved in 7 (58.3%) and showed no marked change in 2 patients (16.7%), with a clinical efficacy of 83.3% (10 / 12). The renal function was improved in 2 (16.7%), stabilized in 6 (50%) and deteriorated in 4 patients (33.3%), with an effective rate of 66.7% (8 / 12). Conclusion For the treatment of atherosclerotic renal artery occlusion, the interventional therapy carries high successful rate and can effectively lower the blood pressure level, in addition, it can also protect the renal function in a certain degree.

AIM: To establish the method of determining cinobufagin and resibufogenin in Xinkening Capsules(Venenum Bufonis,etc.). METHODS: HPLC was applied to determining cinobufagin and resibufogenin.The determination was carried out using an ODS column with a solvent system: methanol-water(50∶40).The flow rate was 1.0 mL/min,detection wavelength was at 296 nm. RESULTS: The linear ranges of cinobufagin and resibufogenin were 3.5 ?g-0.28 ?g and 3.75 ?g-0.30 ?g,respectively.The average recoveries of cinobufagin and resibufogenin were 99.86%(RSD=1.8%,n=5),and 99.84%(RSD=1.2%,n=5),respectively. CONCLUSION: This method is simple,accurate with strong specificity and can be used to control the quality of Xinkening Capsules.

Anthracosis ; diagnosis ; Diagnostic Errors ; Humans ; Lung Diseases ; diagnosis ; Male ; Middle Aged ; Pulmonary Artery

Treatment options of retinoblastoma should be individualized. Patients with early intraocular stage may be managed with focal treatments, including cryotherapy, thermotherapy, laser photocoagulation, brachytherapy and so on. Patients with middle in- traocular stage may be treated with chemoreduction plus focal therapy. In patients with advanced stage, enucleation and chemotherapy are recommended, with the addition of orbital radiation for patients with extraocular retinoblastoma. Patients with systemic metastases require intensive chemotherapy and consolidation with autologous hematopoietic stem cell rescue.

Object To study the low polarity constituents of Gastrodia elata Blume Methods The constituents were isolated by column with prepared TLC, and identified by NMR, MS spectra Results Seven compounds were isolated and identified They were p hydroxybenzyl ethyl ether (Ⅰ), ? sitosterol (Ⅱ), palmitic acid (Ⅲ), 2, 2′ methylenebis (6 tert butyl 4 methylphenl) (Ⅳ), p hydroxy benzyl methyl ether (Ⅴ), dimethyl phthalate (Ⅵ), p hydroxybenz aldehyde (Ⅶ) Conclusion Two compounds were isolated from G elata firstly, and compound Ⅳ as tetra benzol dimer is also isolated from the plants of Gastrodia R. Br. and Orchidaceae for the first time

Diabetes Mellitus ; Diabetic Neuropathies ; drug therapy ; physiopathology ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Inventions