1.Effect of Metformin on Sex Hormone,Insulin Resistance and Related Indexes in Women with Polycystic Ovarian Syndrome
Chunqiong REN ; Yong XIAO ; Yi LI ; Haixia REN
China Pharmacy 2016;27(27):3791-3793,3794
OBJECTIVE:To investigate the effect of metformin on sex hormone level,insulin resistance and related indexes in women with polycystic ovarian syndrome(PCOS). METHODS:79 PCOS women were collected as PCOS group,other 86 healthy women as control group. All patients received 0.85 g Metformin tablet,twice a day,for totally 6 months. Boby mass,BMI,waist circumference,hip circumference,ratio of waist to hip,glycated hemoglobin,T,DHEA-S,E2,LH,FSH,PRL,PROG,SHBG, FPG,FINS,LH/FSH,HOMA-IR and FAI before and after treatment in PCOS group were observed and compared with control group. RESULTS:During treatment,2 patients made withdrawal because of pregnancy,3 made withdrawal because of showing gastrointestinal side effects,2 were lost to follow,totally 72 completed it. After treatment,body mass,BMI,waist circumference, hip circumference,glycated hemoglobin,FINS,HOMA-IR,T,LH,LH/FSH,PROG,FAI in PCOS group were significantly low-er than before,SHBG was significantly higher than before,glycated hemoglobin and PROG were lower than control group,while waist circumference,FINS,HOMA-IR,LH/FSH and FAI were higher than control group,the differences were statistically signifi-cant (P<0.05). There was no significant difference in ratio of waist to hip,FPG,DHEA-S,E2,FSH and PRL before and after treatment(P>0.05). CONCLUSIONS:Metformin can effectively improve the sex hormone levels and insulin resistance of patients with PCOS.
2.Interventional therapy of cavernous hemangioma of the liver(CHL)
Xiao LI ; Weixin REN ; Yulan GU
Journal of Medical Postgraduates 2001;14(2):162-164
This assay reviewed the feeding arteries of CHL and summarized the mechanism,technologic method and common embolization agent of interventional therapy,as well as its effect,indications,contraindications and complications.
3.Effect of propofol on hypoxic pulmonary vasoconstriction in rats
Chinese Journal of Anesthesiology 1995;0(12):-
Objective To investigate the effect of propofol on hypoxic pulmonary vasoconstriction(HPV)and assess the underlying mechanism in rats.Methods Twenty male SD rats weighing 300-400 g were.anesthetizedwith intraperitoneal phenobarbital.Heart and lungs were removed after thoracotomy.Pulmonary.arterial rings 4 mmin length and 1.0-1.4 mm in diameter were prepared and suspended in Earl solution maintained at 37℃ with a pHof 7.40 and aerated with 20% O_2-5% CO_2-25% N_2.The rings were stimulated with phenylephrine(PE)10~(-6)mol?L~(-1) with different preloads(300,500,700,900,1 100 mg).The isometric tension of the arterial rings wasmeasured.The optimal preload was determined to be 900 mg which allowed best contractility.Hypoxia was inducedby aerating the solution with 95% N_2-5% CO_2 and the flow rate was adjusted to maintain PO_2 of the solution at40-20 mm Hg and pH at 7.40.HPV of the rings were recorded.Then propofol was added to achieve a finalconcentration of 1,3,10,30,100 ?mol?L~(-1) and HPV was again induced and the changes in HPV wererecorded.In addition the effects of propofol(10,30 ?mol?L~(-1))on vasoconstriction produced by KCI and PE werealso measured.Results The lower doses of propofol(10 ?mol?L~(-1))significantly inhibited HPV(P
5.The inhibitory action of Bevacizumab on rat corneal neovascularization
Guo-Li, JING ; Xiao-Wei, GAO ; Bing, REN ; Yun, XIAO
International Eye Science 2008;8(10):1955-1958
AIM To evaluate the anti-angiogenesis action of Bevacizumab on corneal neovascularization(CNV) in rats induced by alkali burns.·METHODS: 20 Health Wistar rats, aging from 6 to 8 weeks and weighting from 170g to 190g from 170g to 190g were prepared for CNV animal models. Both corneas of each animal in experimental were cauterized with alkali, then all rats were randomly divided into four groups (each group have 5 rats and 10 corneas), the both corneas of each rats were received subconjunctival Bevacizumab in different dosage (group 2, 0.5rag; group 3, 1.0mg; group 4, 2.0mg)and the group 1 received carrier solution. The occurrence and development ofCNVwereobservedbyslit-lamp microscope, and length and area of CNV were calculated. All rats were followeded up 16 days after alkali burns. The 40 corneas were taken for histopathological examination. Vascular endothelial growth factor (VEGF) were detected in all rat corneas by immunohistochemistry method. ·RESULTS: In the bevacizumabotreated eyes, the vascular area was lower than in the control eyes. The treated group was statistical differences compared with the control group; when vascular area were compared between the treated groups, no statistical differences were observed. The histopathological findings showed that the inflammation cells and the neovascularity in each treated group were significantly fewer than that in the control group. The expression of VEGF markedly increased in CNV control group compared with bevacizumab-injected group. ·CONCLUSION: Subconjunctival application of a certain concentrations Bevacizumab could inhibit angiogenesis in rats corneas induced by alkali burns.
6.Effects of lead exposure in drinking water on hydrogen peroxide, hydroxyl free radicals and lipid peroxidation levels in brain tissues of rats
Yuanmei XIAO ; Qunying XU ; Zhongwei ZHANG ; Wei LI ; Jiangao FENG ; Qingfeng REN ; Xiaohui REN ; Weijuan LI
Tianjin Medical Journal 2015;(10):1119-1121
Objective To explore the effects of lead acetate on free radicals and lipid peroxidation in the cerebral cor?tex, cerebellum, and hippocampus in rat brains. Methods SD rats (n=48), who were just weaned, were randomly divided in?to 4 groups base on their weight. Then the rats were fed with lead acetate in drinking water at the final concentrations of 0 mg/L (deionized water), 200 mg/L, 400 mg/L, 800 mg/L respectively. Blood lead level as well as the hydroxyl free radical inhibiting activity, the levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in cerebral cortex, cerebellum, and hippocam?pus were measured 60 days after lead contamination in water. Results Upon lead exposure, blood lead levels increased sig?nificantly as compared with the control. The hydroxyl free radical inhibiting activity in cerebral cortex, cerebellum, and hip?pocampus decreased significantly in a dose dependent manner of lead(P < 0.05). And they all correlated negatively with blood lead level (r=-0.505,-0.414,-0.448, P<0.05). By contrast, blood lead level was positively correlated with H2O2 and MDA in these brain tissues (r=0.301, 0.411, 0.378, and 0.404, 0.324, 0.510,P < 0.05). Conclusion Lead exposure can lead to lipid peroxidation of rat brain tissues through inducing free radicals.
7.Clinical observation of biopsy by double paths for higher positive percentage.
Hong-Quan WEI ; Gang YU ; Xiao-Tian LI ; Zhong REN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(1):67-68
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Biopsy
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methods
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Child
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Child, Preschool
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Endoscopy
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Female
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Humans
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Larynx
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pathology
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Male
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Middle Aged
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Nasopharynx
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pathology
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Young Adult
8.Study on biological characteristics of adult adipose-derived mesenchymal stem cells and its differentiation into cardiomyocytes in vitro
Gang REN ; Yunfei BIAN ; Xiaojia WU ; Maolian LI ; Chuanshi XIAO
Journal of Chinese Physician 2010;12(7):907-909
Objective To induce adipose-derived mesenchymal stem cells (ADMSCs) differentiation into cardiomyocytes, so as to provide stem cells for myocardial regeneration.Method ADMSCs were isolated and cultured from adult adipose tissue in vitro.They were induced with 10μmol/L 5-azacytidine (5Aza) for 24h.At the 7th, 14th, 21st days after induction, the expression of α-sarcomeric actin, and myosin heavy chain were repeatedly detected by immunocytochemistry.The expression of ANP was detected by RT-PCR.Results At the 7th day after induction, there was no expression of α-sarcomeric actin and MHC.At the 14th day, there was a little expression of α-sarcomeric actin and MHC that could be seen in cells.At the 21st day, there was increased expression of α-sarcomeric and MHC, and the expression of ANP was positive results detected by RT-PCR.Conclusions 5-Aza can induced ADMSCs to differentiate into cardiomyocytes.ADMSCs might be a potential source of cell transplantation for myocardial.
10.Prediction of Long-term Response to Cardiac Resynchronization Therapy by Real-time Three-dimensional Echocardiography
Weidong REN ; Yangjie XIAO ; Chunyan MA ; Shuang LIU ; Xiuyun LI
Journal of China Medical University 2010;(9):752-754
Objective To evaluate the value of real-time three-dimensional echocardiography(RT3DE)to predict the long-term response to cardiac resynchronization therapy(CRT).Methods Twenty-six patients with heart failure were scheduled for CRT.RT3DE was performed before and one year after the pacemaker implantation,and left ventricular(LV)dyssynchrony was defined as the maximum difference(Tmsv 16-Dif)and standard deviation(Tmsv 16-SD)when the minimum systolic volume of 16 segments was reached.Patients were divided into re-sponders and non-responders according to a reduction ≥15 % in LV end-systolic volume(LVESV)after CRT.Results Seventeen patients(65.4 %)were classified as responders.Compared with non-responders,the responders demonstrated a significant increase in LV ejection fraction,and reduction in Tmsv 16-SD.Tmsv 16-SD was a determinant factor for Δ LVESV ≥15%.Tmsv 16-SD 〉3.5% could be employed to evaluate the short-term response to CRT with 80 % sensitivity and 77 % specificity.Conclusions CRT can increase the LV systolic function and synchrony.RT3DE is highly predictive for long-term response to CRT