1.Progresses in studies on residual cardiovascular problems in children with postoperative tetralogy of Fallot.
Chinese Journal of Pediatrics 2004;42(4):311-314
Arrhythmias, Cardiac
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etiology
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Cardiovascular Abnormalities
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etiology
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physiopathology
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Child
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Heart Function Tests
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Heart Septal Defects, Ventricular
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etiology
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Humans
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Postoperative Care
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Postoperative Complications
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Prognosis
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Pulmonary Valve Insufficiency
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etiology
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Tetralogy of Fallot
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surgery
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Time Factors
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Ventricular Outflow Obstruction
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etiology
4.Age-associated alternations in cardiac β-adrenergic receptor signaling
Jing MA ; Shiwen WANG ; Ruiping XIAO
Journal of Geriatric Cardiology 2005;2(4):254-257
During aging, cardiac contractile response to β-AR stimulation is decreased in humans and animal models. Recent studies demonstrate that the positive inotropic effects of both β1-AR and β2-AR stimulation are significantly decreased with aging.This is accompanied by decreases in both β-AR subtype densities and a reduction in membrane adenylyl cyclase activity. However,neither G protein-coupled receptor kinases (GRKs) nor inhibitory G proteins (Gi) appears to contribute to the age-associated reduction in the β-AR modulation of contraction. Thus, while both aging and chronic heart failure exhibit a diminution in cardiac β-AR responsiveness, only heart failure exhibits increased GRK-mediated desensitization ofβ-Ars and an upregulation of Gi proteins.
5.RNA interference and its effect of CYP76AH1 in biosynthesis of tanshinone.
Ying MA ; Xiao-hui MA ; Xiao-jing MA ; Juan GUO ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(8):1439-1443
Tanshinones, the main bioactive compounds of Salvia miltiorrhiza, are the diterpenoid pigments, multiple genes were proved to be involved in their biosynthesis in plants. CYP76AH1 is the initial P450 gene in the tanshinones biosynthetic pathway, its function has been validated by yeast expression and in vitroenzymatic reaction. In order to clarify the function of CYP76AH1 in vivo, in this study, we constructedthe RNA interference of CYP7AH1 in S. miltiorrhiza hairy root. The RNA interference vector with a hairpin structure was constructed using the Gateway technology, and then the interference fragment was integrated into the genome of S. miltiorrhiza mediated by Agrobacterium rhizogenes. Several highly CYP76AH1 interference S. miltiorrhiza hairy roots were obtained for further analysis.
Agrobacterium
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genetics
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metabolism
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Biosynthetic Pathways
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Cytochrome P-450 Enzyme System
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genetics
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metabolism
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Diterpenes, Abietane
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biosynthesis
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Gene Expression Regulation, Plant
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Plant Proteins
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genetics
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metabolism
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RNA Interference
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Salvia miltiorrhiza
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genetics
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metabolism
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microbiology
7.Effect of microencapsulation on the expression of the oxidative stress genes of HepG2 cells and exogenous regulation.
Jing XIAO ; Ying ZHANG ; Weiting YU ; Wei WANG ; Xiaojun MA
Journal of Biomedical Engineering 2014;31(2):373-378
The aim of this research is to investigate the influence of microencapsulation on the expression of the oxidative stress genes and exogenous regulation of HepG2 cells. We compared the expression of hemeoxygenase-1 (HO-1) and glutathione S-transferases-A1 (GST-A1) in HepG2 cells under different culture conditions through real-time PCR. The effects of exogenous antioxidants on cell viability and albumin levels were also evaluated through MTT assay and ELISA assay. The results showed that after culturing for 6 and 16 days, the expression levels of HO-1 in encapsulated cells were approximately 4.9 and 3.1 times higher than that of monolayer cells at the same culture period; As for the expression levels of GST-A1, they were elevated to 11.2 and 33 times of monolayer cells (P < 0.05). Accordingly, we found that NAC at 5-10 mmol/L significantly increased the viability by 40%-70% and the biosynthetic function by 20%-30% in microencapsulated HepG2 cells (P < 0.05). GSH increased the viability of the encapsulated cells by 20%-55% and the biosynthetic function by 15% (P < 0.05). In conclusion, oxidative stress exists in the microcapsules and affects genes expression. Exogenous antioxidants can prevent the inhibition effects of oxidative stress on cellular growth.
Antioxidants
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pharmacology
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Cell Survival
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Gene Expression Regulation
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Glutathione Transferase
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metabolism
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Heme Oxygenase-1
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metabolism
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Hep G2 Cells
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Humans
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Oxidative Stress
8.Molecular docking of chlorogenic acid, 3,4-di-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid with human serum albumin.
Jing ZHOU ; Hongyue MA ; Xinsheng FAN ; Wei XIAO ; Tuanjie WANG
Journal of Integrative Medicine 2012;10(10):1149-54
To investigate the mechanism of binding of human serum albumin (HSA) with potential sensitinogen, including chlorogenic acid and two isochlorogenic acids (3,4-di-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid).
9.Neonatal chondrodysplasia punctata in a case.
Xiu-jing WU ; Li-ping SHI ; Xiao-lu MA
Chinese Journal of Pediatrics 2009;47(3):229-230
10.The understanding level of disease and the way of obtaining disease information of children with leukemia: a qualitative study
Jing HAN ; June LIU ; Qian XIAO ; Xiaolei ZHENG ; Yuehong MA
Chinese Journal of Practical Nursing 2010;26(7):1-4
Objective To describe the understanding level of disease of children with leukemia and their ways of obtaining disease information in order to help nurses and parents to select appropriate content and manners to communicate with children about disease information.Methods In-depth interviews were conducted with 25 children' parents using a descriptive qualitative research method,and the data were analyzed using content analysis.Results Children during remission stage of leukemia had different understanding levels of their disease.Ways of children with leukemia to obtain disease information was correlated with their mental maturity.Conclusions Disease information should be told according to children's age,disease course and level of thinking,and health professionals and parents could provide appropilate ways of obtaining information for children on basis of their mental maturity.