2. Confocal laser scanning microscopy for observing difference in synthesizing exopolysaccharide by highly cariogenic clinical and standard strains of biofilm Streptococcus mutans
Academic Journal of Second Military Medical University 2011;32(4):393-398
Objective To observe the different abilities in synthesizing exopolysaccharides between biofilm cells of Streptococcus mutans (S. mutans) clinical isolate 593 and standard stain S. mutans ATCC 25175 (serotype c). Methods S. mutans biofilm specimens were formed on the polystyrene plastic sheets for 3, 12and 20 h. The exopolysacchasides was stained with fluorescein isothiocyanate-conjugated concanavalin A and were visualized by confocal laser scanning microscopy. The amounts of exopolysaccharides produced by adhesive S. mutans in 3-24 h were determined by the anthrone method. Results The green fluorescence in strain 593 group was stronger and wider than that in the S. mutans ATCC 25175 group, with significant differences found for the amounts of water-soluble glucans during 3-20 h and water-insoluble glucans during 3-16 h (P<0.05). Conclusion The synthesis of exopolysaccharides during formation of biofilm increases with time in both clinical and standard strains of S. mutans. However, the stronger synthesizing ability of strain 593 in the early biofilm formation stage (3-16 h) may be the reason for its higher cariogenic ability, indicating that the clinical isolates may be more sensitive in studying the mechanism of caries pathogenesis.
4.Progresses in studies on residual cardiovascular problems in children with postoperative tetralogy of Fallot.
Chinese Journal of Pediatrics 2004;42(4):311-314
Arrhythmias, Cardiac
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etiology
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Cardiovascular Abnormalities
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etiology
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physiopathology
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Child
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Heart Function Tests
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Heart Septal Defects, Ventricular
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etiology
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Humans
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Postoperative Care
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Postoperative Complications
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Prognosis
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Pulmonary Valve Insufficiency
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etiology
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Tetralogy of Fallot
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surgery
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Time Factors
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Ventricular Outflow Obstruction
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etiology
5.Clinical Observation of Wenjing Shexue Recipe in Treating Polycystic Ovarian Syndrome
Yanhui HUANG ; Xiaobin LI ; Jing XIAO
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(3):418-422
Objective To observe the clinical efficacy of Wenjing Shexue Recipe ( WSR) in treating polycystic ovarian syndrome ( PCOS) with liver-kidney deficiency, and to explore the possible therapeutic mechanism. Methods Seventy-four PCOS patients were randomized into observation group ( N=38) and control group ( N=36). The observation group was given oral use of WSR and the control group was treated with Ethinylestradiol and Cyproterone Acetate Tablets orally. Both groups received treatment for 6 months, and were followed up for 3 moths after drug withdrawal. After treatment, the therapeulic effect was evaluated , and the changes of body mass index ( BMI) , hairiness. acne, and sexual hormone levels were monitored before and after treatment. Results ( 1) The total effective rate of the observation gorup was 92.11% and that of the control group was 75.00%, the difference being significant ( P<0.05). ( 2) After treatment for 6 months, body mass index (BMI), the scores of hairiness and acne, and serum levels of testosterone (T) and luteotrophic hormone (LH) were decreased in the observation group (P<0.05 compared with those before treatment). The control group had lower scores of hairiness and acne and serum levels of T and LH than those before treatment ( P<0.05) , but BMI showed no obvious decrease (P>0.05) . The results of follow-up showed that BMI, the scores of hairiness and acne, and serum levels of T and LH were still lower in the observation group than those before treatment (P<0.05), but the above indexes except acne scores were increased in the control group (P>0.05 compared with those before treatment) and were higher than those of the observation group (P<0.05). Conclusion WSR exerts better effect for the treatment of PCOS with liver-kidney deficiency than Ethinylestradiol and Cyproterone Acetate Tablets, and the possible mechanism is probably related with the regulation of endocrine disturbance by decreasing serum T and LH levels.
8.Clinical Observation of Wenyang Huayu Sanjie Decoction in Treating Adenomyosis with Syndrome of Blood Deficiency and Cold Stagnation
Yanhui HUANG ; Jing XIAO ; Liqun LIU
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(5):834-837,842
Objective To observe the therapeutic effect of Wenyang Huayu Sanjie Decoction ( WHSD) for the treatment of adenomyosis with the syndrome of blood deficiency and cold stagnation. Methods Seventy qualified patients were evenly randomized into observation group and control group. The control group was treated with Mirena intrauterine contraceptive device, and the observation group was treated with WHSD ( composed of Danggui Sini San, Wuzhuyu Shengjiang Tang, Shixiao San and Rhizoma Sparganii, Rhizoma Curcumae). The clinical outcomes included the menstrual blood volume, menstrual cycle, menstruation, degrees of dysmenorrhea, uterine volume, and serum CA125 level in the two groups before treatment and 3, 6 months after treatment. Results ( 1) After treatment for 6 months, the total effective rate of the observation group was 94.29%, significantly higher than 85.71% in the control group ( P<0.05). ( 2) After treatment, dysmenorrheal degrees, menstrual blood volume, menstrual cycle, menstruation, uterine volume, and serum CA125 level were improved in both groups ( P<0.05) , and the observation group had better effect on relieving dysmenorrhea, improving menstruation, and decreasing CA125 level than the control group ( P<0.05). ( 3) No obvious adverse reaction was found in the observation group, but most of the cases in the control group had menstrual disorder such as vaginal irregular bleeding and prolongation of menstruation. Conclusion WHSD has certain therapeutic effect on adenomyosis with the syndrome of blood deficiency and cold stagnation, and can also obviously improve the quality of life of the patients.
9.Demands for cardiac rehabilitation information in patients with coronary atherosclerotic heart disease and influential factors
Jing XIAO ; Lingzhi HUANG ; Lezhi LI
Journal of Central South University(Medical Sciences) 2017;42(8):973-978
Objective:To investigate the demands for cardiac rehabilitation information in patients with coronary atherosclerotic heart disease (CHD) and influential factors.Methods:Information demands for cardiac rehabilitation in CHD patients were surveyed by questionnaire and the influential factors were analyzed by one-way ANOVA and multi-factor analysis of variance.Results:The score of demands for cardiac rehabilitation information in CHD patients was 3.86±0.53.Among them,the most urgent top 5 items were:drug knowledge,diagnosis and treatment,basic knowledge of the heart,emergency and safety and nutrition knowledge.The top 3-demand modes were:communication with medical workers,movies or videos to take home,and lectures.The score of demands for cardiac rehabilitation information was different in different age groups.The highest score was in the patients with age less than 60.There were different demands in different characteristic groups.Conclusion:The most urgent need and mode are drug knowledge and communication with medical workers,respectively.With the age increase,the demands for patients' cardiac rehabilitation information decrease.An individualized health education strategy should be developed according to the characteristics of CHD patients.
10.Effect of surgery-lowing intraocular pressure on optic nerve head parameters and retinal nerve fiber layer thickness
Hui, XIAO ; Xing, LIU ; Jing-jing, HUANG ; Zhen, MAO
Chinese Journal of Experimental Ophthalmology 2013;(5):465-470
Background Lowing intraocular pressure (IOP) is the only effective treating method in glaucoma.Making sure the change of optic disc parameters and retinal nerve fiber layer thickness(RNFLT) in primary open-angle glaucoma (POAG) patients after IOP reduced can provide objective evidence for evaluating the effect of lowing-IOP on the function of optic nerve.Objective The present study was to evaluate the change of optic disc parameters and RNFLT in POAG patients after trabeculectomy,and analyze the affecting factors of image parameters.Methods A serial case-observational study was adapted.Forty eyes of 39 patients with POAG underwent trabeculectomy were recruited in the study.IOP was examined and perimetry was performed before operation and 2 weeks,1 month,3 months,6 months and 1 year after surgery.Optical parameters and RNFLT were measured using Heidelberg retina tomography (HRT) and optical coherence tomography (OCT) in above-mentioned time points.The correlations between lowing-IOP range or disease severity and optical disc parameters or mean RNFLT (mRNFLT) were analyzed by Pearson linear correlation.Results Mean IOP of the 40 eyes was (32.77±8.64) mmHg before operation and (12.45 ±3.65) mmHg,(13.05±3.90) mmHg,(13.42± 3.33) mmHg,(14.22± 2.60) mmHg and (14.74±2.46)mmHg 2 weeks,1 month,3 months,6 months and 1 year after operation,with a significant difference among various time points (F=13.73,P =0.00).However,no significant difference was seen in the mean defect of perimetry among the different time points (F=1.02,P=0.41).The cup area (CA),cup/disc area ratio (C/DAR),cup volume (CV),mean cup depth (MCD),cup shape measurement (CSM),horizontal C/D,vertical C/D,rim area (RA),rim/disc area ratio (R/DAR),height variation contour (HVC),rim volume (RV),mRNFLT,RNFL cross sectional area (RNFLA) were gradually improved after operation in comparison with before operation.One year after operation,RV,CSM,mRNFLT,RNFLA were still increased,but vertical C/D was reduced after operation than that of before (P<0.05).No significant differences were found in the whole mRNFLT and superior quadrant mRNFLT,inferior quadrant mRNFLT,nasal quadrant mRNFLT and temporal quadrant mRNFLT among before and after operation (F=1.63,P=0.16; F=0.51,P=0.77; F=1.44,P=0.20;F=1.02,P=0.32; F=1.31,P=0.30).Positive correlations were found between lowing-IOP range and the improvement of RV,CSM,vertical C/D (r =0.45,P =0.02; r=0.41,P=0.04; r=0.58,P=0.00),and negative correlations were seen between absolute value of MD and the improvement of RV,CSM 1 year after operation (r=-0.43,P=0.03; r=-0.62,P=0.00).Conclusions Reduction of IOP after trabeculectomy results in the reversal of optic disc parameters.The improvement of the optic disc parameters may be a protective factor for progression of glaucomatous optical nerve damage.