Adolescent ; Adult ; Darier Disease ; therapy ; Electroacupuncture ; Female ; Humans ; Young Adult

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Isocitrate Dehydrogenase ; genetics ; Leukemia, Myeloid, Acute ; genetics ; Male ; Mutation ; Nuclear Proteins ; genetics ; Prognosis ; Treatment Outcome

Objective To observe the effect of Manzhi Kechuan Ling(MKL)on pulmonary function and quality of life (QOL)of patients with chronic obstructive pulmonary disease (COPD)at stationary stage.Methods Seventy-six COPD patients were randomized into two groups.The treatment group (N=40)received oral use of MKL (mainly composed of Radix Ginseng,Radix Astragali,Rhizoma Atractylodis Macrocephalae,Colla Cornus Cervi,Placenta Hominis,Gecko,Semen Juglandis,Gecko,Fructus Perillae,Bulbus Fritillariae Cirrhosae,Rhizoma Pinelliae)for 6 months,and was followed up for another 6 months after suspension of medication.The control group (N=36)did not receive any medication.The pulmonary function,TCM syndrome scores,QOL scores and annual acute attack times were examined before treatment,6 and 12 months after treatment.Meanwhile,artery blood gas analysis was performed before treatment and 6 months after treatment.Results Inthe treatment group,the pulmonary function indexes of forced expiratory volume in one second (FEV1)andforced vital capacity (FVC)were improved 6 months after treatment (P0.05).In the control group,FEV1 and FVC presented a decreasing trend 6 and 12 months after treatment,and FEV1 and FCV 12 months after treatment differed from those before treatment (P0.05).There showed statistical differences of PaO2 and PaCO2 between the two groups 6 months after treatment(P

Objective To investigate the therapeutic effect of Shouling Jiannao Capsule (SJC)for the treatment of multiple infarction dementia (MID),and to observe the effect of SJC on blood lipid level and hemorrheology.Methods Fifty MID patients aged 45~80 subjecting to the diagnostic standards were randomly divided into two groups:treatment group (N=30)received SJC (mainly composed of Radix Polygoni Multiflori,Ganoderma Lucidum,Radix Notoginseng,Radix Salviae Miltiorrhizae and Radix Curcumae),and control group (N=20)received dihydroergotoxine.The treatment lasted 2 months.The therapeutic effect in both groups was evaluated after treatment,and the changes of scores of TCM syndromes,Hasegava Dementia Scale(HDS),and Mini-mental State Examination (MMSE)were observed before and after treatment.Meanwhile,blood lipid level,hemorheological indexes,erythrocyte superoxide dismutase(SOD),plasma lipid peroxide(LPO)and transcranial color Doppler(TCD)were examined and statistically analyzed before and after treatment in both groups.Results After treatment,the total effective rate was 90.0% in the treatment group and 55.0% in the control group,the difference being significant(P

Aim To study the mechanism of metformin in the treatment of type 2 diabetic melitus (T2DM) rats. Methods Rats were randomly divided into normal group, model group and metformin group with 10 rats in each group. Four weeks after induction with HFSD, 35 mg 'kg-1 STZ was injected intramuscularly, and the experiment was completed after 8 weeks of administration. UPLC/ESI-TOF-MS was used to study the effects of metformin on metabolites in serum of T2DM rats, and qPCR was used to find its target. Results Compared with normal group, the body weight, pancreatic index and insulin level in model group decreased significantly (P < 0. 05) , liver index, GLU, TC, TG and FFA levels increased significantly (P < 0.01), OGTT was significantly impaired, while met-formin could significantly increase body weight, pancreatic index and insulin level (P <0. 05) , reduce liver index, GLU, TC, TG and FFA levels (P < 0. 05) , and improve OGTT. After metahonomics, 17 biomark-ers were obtained. After verification, metformin was found to regulate the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids. Conclusions Metformin can improve the lipid metabolism disorder of T2DM by regulating the synthesis and metabolism of cholesterol, fatty acids, bile acids and phospholipids.

We pretreated sawdust (Castanopsis fissa Rehd.et Wils) by solid state fermentation (SSF) with Phanerochaete chrysosporium, and then compressed it into pellets with the moisture content of 15% and the pressure of 98 MPa, to solve the problem of low density, low Meyer hardness, high water uptake, and short storage period of pellet in the woody pellet industry. We studied the effects of fermentation time on pelletization and pellets's characteristics (including energy consumption, density, Meyer hardness, and hydrophobicity). SSF affected the heating values of pellet. Compared with fresh sawdust, SSF consumed more energy at the maximal value by 6.98% but saved extrusion energy by 32.19% at the maximum. Meanwhile, SSF could improve the density, Meyer hardness and hydrophobicity of pellet. Pellet made of sawdust pretreated by SSF for 48 d had best quality, beneficial for long-term transportation and storage of pellets.
Biofuels ; Fermentation ; Hydrophobic and Hydrophilic Interactions ; Phanerochaete ; Water ; Wood

Objective To study the effect of antiapoptosis factors PED/PEA-15 and XIAP on prostate cancer cells(PC-3)apoptosis.Methods The expressions of XIAP and PED/PEA-15 in prostate cancer cells(PC-3)were respectively assayed using the RT-PCR technique.XIAP and PED/ PEA-15 specific siRNA vectors were designed and constructed and then were transiently cotransfected into PC-3 cells under induction of liposome.The effects of siRNA vectors on PED/PEA-15 and XIAP transcription were assayed by RT-PCR technique,and the effect of XIAP and PED/PEA-15 on cancer cell apoptosis were determined by flow cytometry and microscope observation.Results PED/PEA- 15 and XIAP were both highly expressed in PC-3 cells.Enzyme digestion analysis and DNA sequencing confirmed that the PED/PEA-15 and XIAP-specific siRNA expression vectors were constructed successfully.The designed siRNA sequences of PED/PEA-15 and XIAP could specifically inhibit their transcription.The PC-3 cells which were cotransfected with PED/PEA-15 and XIAP- specific siRNA vectors were more sensitive to doxorubicin.The apoptosis rate of cotransfected cells was significantly increased.Conclusions PED/PEA-15 and XIAP might be involved in the development of prostate cancer.

Hernia, Inguinal ; diagnostic imaging ; Humans ; Male ; Middle Aged ; Peritoneal Dialysis, Continuous Ambulatory ; Peritoneum ; diagnostic imaging ; Tomography, X-Ray Computed ; methods

OBJECTIVEIn this study, five heavy metals contamination of soil and different parts of Panax notoginseng in the plantation area was investigated. Analysis of heavy metals correlation between the planting soil and P. notoginseng; and the absorption and accumulation characteristics and translocation of soil heavy metals by P. notoginseng plants was revealed.

METHODThrough field investigation and laboratory analytical methods, analysis of China's 30 different soil P. notoginseng origin and content of heavy metals in five different parts of the P. notoginseng plant content of heavy metals.

RESULTThe results revealed that the soil heavy metals should not be neglected in the plantation area Referring to the national soil quality standards (GB15608-1995), the excessive degree of soil heavy metals pollution showed Hg > As > Cd > Cr in the plantation area, and Pb content of soil was in the scope of the standard. Refer to 'Green Industry Standards for Import and Export of Medical Plants and Preparations', the excessive degree of heavy metals content of P. notoginseng plants showed As > Pb > Cr > Cd, and Hg content of plants was in the scope of the standard. Concentrations of five heavy metals of underground parts of P. notoginseng plants are higher than aboveground, and heavy metals elements are more concentrated in the root, followed by the rhizome of P. notoginseng plants. Heavy metal accumulation characteristics of the different parts of the P. notoginseng of the overall performance is the root > the rhizome > the root tuber > leaves > stems. From the point of view BCF value analysis of various parts of the P. notoginseng plants to absorb heavy metals in soil, BCF values of all samples were less than 1, description P. notoginseng not belong Hyperaccumulator. From the view of transportation and related analysis of the soil-P. notoginseng systems, the rhizome of P. notoginseng and the content of As and Cr in soil was significantly correlated, the root of P. notoginseng and the content of Cd in soil was significantly correlated, and no significant correlation between the other indicators. Through the analysis of transportation transfer coefficient showed: Pb, As and Cr are not easy to transport aboveground part from the underground, but Cd and Hg are relatively easy to transport stems from rhizome, the migration of five heavy metals in the aerial part is relatively strong, and heavy metal of stems is easily transported to the leaves.

CONCLUSIONP. notoginseng does not belong to the enrichment of heavy metals in crops, especially for Hg in soil with strong patience. In survey area, the content of heavy metals of P. notoginseng's planting soil is relatively high, and the heavy metals As, Pb, Cr, Cd of P. notoginseng also exist heavy metals exceeded problems. Due to the presence of heavy metals in crops internal absorption and translocation of special laws, accumulation of heavy metals varied significantly in different parts of P. notoginseng. The overall, the performance for the heavy metal content of the underground parts is more than aboveground, it explain heavy metals of P. notoginseng plants is still the main source of the soiL Therefore, the key to control of planting area soil environmental quality and reduce exogenous harmful substances secondary pollution of soil in the cultivation process are to study and solve the heavy metals pollution problem of P. notoginseng.

Adsorption ; China ; Laboratories ; Metals, Heavy ; analysis ; Panax notoginseng ; chemistry ; Soil ; chemistry ; Soil Pollutants ; analysis

Acquired chromosomal translocations can be identified in nearly 50% of human acute myeloid leukemias. The common chromosomal translocation in this disease is t(8;21) (q22;q22). It involves the aml1 (runx1) gene on chromosome 21 and the eto (mtg8, runx1t1) gene on chromosome 8 generating the aml1/eto fusion gene. An initial model for its pathogenesis emphasized the conversion of a hematopoietic transcriptional activator AML1 into a leukemogenic repressor which blocked myeloid differentiation at the level of target gene regulation. Aml1/eto fusion genes inhibit key hematopoietic transcription factor that function as tumor suppressors at several nodal point during hematopoietic differentiation. A new model is presented in which aml1/eto coordinates expansion of the stem cell compartment with diminished lineage commitment and with genome instability. In this review, the molecular role of aml1/eto fusion gene and his transcribed isoforms in regulating stem renewal, blocking hematopoietic differentiation and interacting with various lineage-specific transcription factors are summarized.
Chromosomes, Human, Pair 21 ; Chromosomes, Human, Pair 8 ; Core Binding Factor Alpha 2 Subunit ; genetics ; Humans ; Leukemia, Myeloid, Acute ; genetics ; pathology ; Oncogene Proteins, Fusion ; genetics ; RUNX1 Translocation Partner 1 Protein