1.Impact of High Pulmonary Blood Flow on Content and Metabolism of Collagen in Rats
xiao-hui, LI ; jun-bao, DU ; chao-shu, TANG
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To explore impact of high pulmonary blood flow on the content and metabolism of collagen in rats.Methods Thirty-two male SD rats were randomly divided into shunt group and control group.Rats in shunt group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary blood flow.In control group,rats experienced the same expe-rimental processes except the shunting procedure.After 4 and 11 weeks of experiment,these changes of pulmonaryartery collagen Ⅰ,collagen Ⅲ,matrix metalloproteinase(MMP-13)and tissue inhibitor of metalloproteinase(TIMP-1) protein expression of rat were investigated by immunohistochemistry.Results After 4 weeks and 11 weeks of shunt,the collagen Ⅰ,collagen Ⅲ,MMP-13 and TIMP-1 of pulmonary artery in rats of shunt group increased significantly compared with those of control group,respectively(all P
2.Oxaliplatin combined fluorouracil and calcium folinate in the treatment of 37 cases with advanced colorectal cancer
Xiao-Bo DU ; Hong WANG ; Hui LI ; Al ET
China Oncology 2001;0(05):-
Purpose:To study the response and toxicity of the regimen of oxaliplatin combined with fluorouracil and calcium folinate in the treatment of consisting of advanced colorectal cancer.Methods:Thirty-seven patients with advanced colorectal cancer received chemotherapy of regimen:oxaliplatin 130 mg/m~2 2 hours iv on day 1,calcium folinate 200 mg/ m~2 iv 2 hours on days 1 to 5,followed by fluorouracil 300 mg/m~2(≤500 mg/d) iv 4 h on days 1 to 5,three or four weeks as one cycle.Results:The total response rate was 29.7%,the main toxicity was bone marrow suppression and neuro-sensory toxicity,leukopenia was observed in 45.9% of the patients,but grade Ⅲ and Ⅳ in only 8.1%,neuro-sensory toxicity was observed in 81.9.%,but grade Ⅲ and Ⅳ in only 5.4%.Conclusions:This study shows that the regimen of oxaliplatin combined fluorouracil and calcium folinate is effective and tolerable in advanced colorectal cancer therapy.
3.Analytical method for benzene, toluene, xylene, hexane, trichloroethylene in gooey agent by gas chromatography.
Hui-Fang YAN ; Xiao-Shuang XIE ; Hui-Fang DU ; Jing ZHANG ; Chun-Ling LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(4):303-305
Benzene
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analysis
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Chemical Industry
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Chromatography, Gas
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methods
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Humans
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Occupational Exposure
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analysis
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Toluene
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analysis
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Trichloroethylene
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analysis
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Xylenes
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analysis
4.Research progress of small molecular drugs targeting RNA
Xiao-li DU ; Hui-hui CHEN ; Xiang-yang YE ; Tian XIE ; Xing-rui HE
Acta Pharmaceutica Sinica 2022;57(10):2902-2913
At present, majority of the small molecular drugs used in clinics target proteins, they exert the efficacy through the binding to specific sites on the target protein. However, the "druggable" protein targets account for a small portion of the total number of proteins, and "non-druggable" proteins account for 80%, because of not having suitable drug binding sites. In the central rule, RNA is located in the upstream of proteins and controls the transcription of proteins. The research of small molecule drugs targeting RNA can solve the problem of protein "undruggable proteins" in some extent. This review summarizes the representative research achievements of small molecular drugs targeting RNA in recent years, and the screening methods applied to this field, with the focuses on the latest progress of small molecular drugs targeting novel coronavirus RNA.
5.Effect of oxidative stress on ventricular arrhythmia in rabbits with adriamycin-induced cardiomyopathy.
Li, HE ; Jianmin, XIAO ; Hui, FU ; Guangsheng, DU ; Xing, XIAO ; Cuntai ZHANG ; Ye, GU ; Yexin, MA
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(3):334-9
The purpose of the present study was to examine the effects of oxidative stress on ventricular arrhythmias in rabbits with adriamycin-induced cardiomyopathy and the relationship between oxidative stress and ventricular arrhythmia. Forty Japanese white rabbits were randomly divided into four groups (n=10 in each): control group, metoprolol (a selective β1 receptor blocker) group, carvedilol (a nonselective β blocker/α-1 blocker) group and adriamycin group. Models of adriamycin-induced cardiomyopathy were established by intravenously injecting adriamycin hydrochloride (1 mg/kg) to rabbits via the auri-edge vein twice a week for 8 weeks in the adriamycin, metoprolol and carvedilol groups. Rabbits in the control group were given equal volume of saline through the auri-edge vein. Rabbits in the metoprolol and carvedilol groups were then intragastrically administrated metoprolol (5 mg/kg/d) and carvedilol (5 mg/kg/d) respectively for 2 months, while those in the adriamycin and control groups were treated with equal volume of saline in the same manner as in the metroprolol and carvedilol groups. Left ventricular end diastolic diameter (LVEDd) and left ventricular ejection fraction (LVEF) were measured by echocardiography. Plasma levels of N-terminal pro B-type natriuretic peptide (NT-proBNP), malondialdehyde (MAD) and superoxide dismutase (SOD) were detected. The left ventricular wedge preparations were perfused with Tyrode's solution. The transmural electrocardiogram, transmural action potentials from epicardium (Epi) and endocardium (Endo), transmural repolarization dispersion (TDR) were recorded, and the incidences of triggered activity and ventricular arrhythmias were obtained at rapid cycle lengths. The results showed that TDR and the serum MDA and NT-proBNP levels were increased, and LVEF and the serum SOD level decreased in the adriamycin group compared with the control group. The incidences of triggered activity and ventricular arrhythmia were significantly higher in the adriamycin group than those in the control group (P<0.05). In the carvedilol group as compared with the adriamycin group, the serum SOD level and the LVEF were substantially increased; the TDR, and the serum MDA and NT-proBNP levels were significantly decreased; the incidences of triggered activity and ventricular arrhythmia were obviously reduced (P<0.05). There were no significant differences in the levels of MDA and SOD, LVEF, TDR and the incidences of triggered activity and ventricular arrhythmia between the adriamycin group and the metoprolol group. It was concluded that carvedilol may inhibit triggered activity and ventricular arrhythmias in rabbit with adriamycin-induced cardiomyopathy, which is related to the decrease in oxygen free radials.
6.Impact of endogenous hydrogen sulfide on the content of pulmonary artery collagen in rats with high pulmonary blood flow.
Xiao-Hui LI ; Jun-Bao DU ; Chao-Shu TANG
Chinese Journal of Applied Physiology 2008;24(2):216-219
AIMTo explore the possible impact of endogenous hydrogen sulfide (H2S) on the content and metabolism of collagen in rats with high pulmonary blood flow.
METHODSThirty-two male SD rats, weighing 120-140 g, were randomly divided into 4 groups (n = 8), shunt group, shunt + PPG (propargylglycine, an antagonist of endogenous H2S producing enzyme) group, sham group and sham + PPG group. Rats in shunt group and shunt + PPG group were subjected to an abdominal aorta-inferior vena cava shunt to create an animal model of high pulmonary flow. In the sham group and sham + PPG group, rats experienced the same experimental processes except the shunting procedure. After 4 weeks of experiment, lung tissue H2S content of rat was determined by a modified sulfide electrode method. Pulmonary artery collagen I, collagen III, MMP-13 and TIMP-1 protein expressions of rat were investigated by immunohistochemistry.
RESULTSAfter 4 weeks of experiment, lung tissue H2S content increased significantly in rats of shunt group as compared with that of sham group (P < 0.05). Pulmonary artery collagen I and collagen III protein expression increased obviously in rats of shunt group as compared with that of sham group (P < 0.01). After administration of PPG for 4 weeks, lung tissue H2S content decreased significantly in rats of shunt + PPG group as compared with that of shunt group (P < 0.05). In contrast to rats in shunt group, collagen I and collagen III protein expression in pulmonary arteries of shunt + PPG group increased significantly, respectively (P < 0.05). Compared with rats of shunt group, pulmonary artery MMP-13, TIMP-1 and the ratio of MMP-13/TIMP-1 in shunt + PPG group down-regulated significantly (P < 0.05).
CONCLUSIONEndogenous H2S might play a protective regulatory role in the development of pulmonary hypertension and pulmonary vascular structural remodelling in rats by decreasing the content of pulmonary artery collagen resulting from catabolism of collagen.
Animals ; Collagen ; metabolism ; Hydrogen Sulfide ; metabolism ; Lung ; blood supply ; metabolism ; Male ; Matrix Metalloproteinase 13 ; metabolism ; Pulmonary Artery ; metabolism ; Rats ; Rats, Sprague-Dawley ; Tissue Inhibitor of Metalloproteinase-1 ; metabolism
7.Application and expectation of robot-assisted operation system in gastrointestinal surgery.
Chinese Journal of Gastrointestinal Surgery 2012;15(8):778-780
With the development of minimally invasive surgery in China, the da Vinci surgical system was used more and more widely in gastrointestinal surgery. This paper summarizes the development history of the artificial intelligence-assisted surgery system, the application and in the promotion of gastrointestinal surgery in China. Application of this new technology promotes further the innovation and development of minimally invasive surgery, and will drive leap in the field of gastrointestinal minimally invasive surgery.
Digestive System Surgical Procedures
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methods
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Humans
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Minimally Invasive Surgical Procedures
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Robotics
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Surgery, Computer-Assisted
8.Study on pharmacokinetics of geniposide in mice administrated by xingnaojing microemulsion and mPEG2000-PLA modified xingnaojing microemulsion.
Ran WEN ; Xiao-Lan CHEN ; Hui-Yun LI ; Qing-Li GUO ; Yang LU ; Shou-Ying DU
China Journal of Chinese Materia Medica 2014;39(6):1111-1114
An HPLC method for the determination of geniposide concentration in mouse plasma was developed and the pharmacokinetics after intranasal administration of Xingnaojing microemulsion (XNJ-M) and mPEG2000-PLA modified Xingnaojing microemulsion (XNJ-MM) were investigated. Eighty mice were treated by XNJ-M and XNJ-MM nasally. The plasma samples were collected at different times and the drug in samples was detected by HPLC. The pharmacokinetic parameters were calculated by the software of Kinetica. The pharmacokinetic parameters of geniposide of XNJ-M were C(max) (4.36 +/- 2.69) mg x L(-1), t(max) 1 min, MRT (29.73 +/- 4.54) min, AUC (53.63 +/- 14.03) mg x L(-1) x min. The pharmacokinetic parameters of geniposide of XNJ-MM were C(max) (9.75 +/- 4.14) mg x L(-1), t(max) 1 min, MRT(22.34 +/- 2.90) min, AUC (131.87 +/- 40.13) mg x L(-1) x min. Geniposide can be absorbed into blood in a higher degree after intranasal administration with XNJ-MM compared to XNJ-M, which maybe caused by its less irritating and more absorption.
Animals
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Drugs, Chinese Herbal
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administration & dosage
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chemistry
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Emulsions
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Iridoids
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blood
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pharmacokinetics
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Lactic Acid
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chemistry
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Male
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Mice
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Polyesters
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Polyethylene Glycols
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chemistry
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Polymers
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chemistry
9.Intracerebroventricular administrations of angiotensin IV (Ang IV) ameliorate cognitive disorder in diabetic rats
Hui-Min Du ; -Lin Niu MM ; Ke-Xiang Zhao ; Juan Li MMS ; Qian Xiao
Neurology Asia 2013;18(2):195-202
Cognitive impairment is a common complication of diabetes. Hippocampus plays an important role
in cognitive function. In hyperglycemia, synaptophysin, a crucial synaptic vesicle membrane protein
in hippocampus neuron is found to be down-regulated. Recent evidences have shown that angiotensin
IV can facilitate memory acquisition and recovery. However, whether it can also improve cognitive
functions of diabetic rats with cognitive disorder, and the possible mechanisms are uncertain. Hence,
the objectives of this study. Forty fi ve Sprague Dawley male rats were randomly divided into three
groups: Control, diabetic group and diabetes with angiotensin IV treatment group. The cognitive
functions, mainly learning and memory of the rats were evaluated using Morris water maze task. The
synapses ultrastructure, relative mRNA concentrations and protein expression levels of synaptophysin
in hippocampus CA1 area were estimated using transmission electron microscope, RT-PCR,
immunohistochemistry and western blotting, respectively. Our study showed that in the diabetic rats with
angiotensin IV treatment, the cognitive impairment as measured by Morris water maze task improved,
the ultrastructure of synapses in hippocampus reversed, the relative mRNA concentrations and protein
levels of synaptophysin in hippocampus signifi cantly increased, when compared with diabetic rats.
We conclude that angiotensin IV plays an important role in improving cognitive function of diabetic
rats. The possible mechanisms are up-regulating the expression of synaptophysin and normalizing the
ultrastructure of synapses in hippocampus.
10.Relationship between Serum Platelet Derived Growth Factor-BB and IgA Nephropathy in Children
gui-mei, DU ; fu-xiao, WU ; xiu-qiao, TIAN ; bing-hui, XU
Journal of Applied Clinical Pediatrics 2006;0(17):-
Objective To investigate the clinicopathologic relationship between serum platelet derived growth factor-BB(PDGF-BB)and IgA nephropathy in children.Methods The level of serum PDGF-BB was detected by double antibody enzyme linked immunosorbent assay(ELISA) in 15 cases healthy children and 30 cases IgA nephropathy.According to patholgical degree constituted by WHO in 1982,the IgA nephropathy group divided into 5 degrees:Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ(Ⅰ,Ⅱ were light patholgic change group;Ⅲ,Ⅳ were moderate patholgic change group;Ⅴ was severe patholgic change group).The serum PDGF-BB in IgA nephropathy group and none-IgA nephropathy group,and in different renal pathology type IgA nephropathy group were analyzed.Data were analyzed by using SAS 6.12 software.Results The level of serum PDGF-BB were(247.35?55.79) ng/L in control group and(869.16?200.73) ng/L in IgA nephropathy group.It was higher in IgA nephropathy group than in control group,the difference was significant(P