Animals ; Antineoplastic Agents ; therapeutic use ; Benzamides ; Dasatinib ; Drug Resistance, Neoplasm ; drug effects ; Fusion Proteins, bcr-abl ; genetics ; metabolism ; Humans ; Imatinib Mesylate ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; drug therapy ; genetics ; metabolism ; Piperazines ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; Pyrimidines ; pharmacology ; therapeutic use ; Thiazoles ; pharmacology ; therapeutic use

Objective To study the relationship between the expression of matrix metallopro-teinase- 2(MMP- 2) and the clinical pathological characteristics of laryngeal squamous cell carcinoma.Methods Irnmunohistochemistry technique (ABC) was used to detect the expression and location ofMMP- 2 in the tissue of 43 cases with laryngeal squamous cell carcinoma. ResultsThe positive ex-pression rate of the MMP - 2 protein was 29(67.4% ) among the 43 laryngeal tumor tissues. It was sig-nificantly related to metastasis of the cervical lymph nodes (P<0.05). MMP- 2 was immunolocalizedon the membranes and intracytoplasm of metastatic cluster tumor cells or the tumor cells adjacent to thebasement membrane. ConclusionThese results suggest that MMP- 2 plays an important role in thetumor invasion and metastasis of laryngeal squamous cell carcinoma.

To identify the expression of the molecule recognized by Pf18-3 mAb (Pf18-3 molecule) on various cells. Meth-ods: The expression of pr18-3 molecule was assayed by flow cytometry and confocal laser scanning microscope . Result: The molecule recog-nized by Pf18-3 mAb expressed on TSC and other stromal cells. Whereas, fresh thymocytes were Pf18-3 negative. Interestingly, the expressionof Pf18-3 molecule was gradually up-regulated on thymocytes after activation by ConA. This molecule mainly expressed on CD4+ CD8+ andCD4+ CD8- cells. Under confocal laser scanning microscope, the staining of fluorescence showed as ring around the cell, it changed grsduallystronger and thicker with activation. Conclusion: This study indicated that the Pf18-3 molecule was co-expressive molecule of MTSC and acti-vated tlymocytes,it was concemed closely about the activation of CD4+ C D8+ and CD4+ CD8- cells.

Objective To evaluate the clinical effect of the enteral nutrient(Pediasure) on making improvements in nutritional status of children with anorexia.Methods Thirty children were treated with pediasure for 2 months, twice a day, and height(Ht),weight(Wt),AG,TSF,SSF,ASF,RBC,Hb were measured before and after treatment and their appetites,alimentary canal reactions were recorded.Results Compared with the effects after 2 months, the average Ht,Wt,AG,SF all increased, and there was significant difference between pretreatment and after treatment(P0.05). All of the 30 children had no adverse reactions during treatment.Twenty-two children (73.33%) had better appetites and increased the amount of eating after treatment.Conclusion The study suggests that pediasure may be safely used in the treatment of children′s anorexia and effectively improve patients′ nutrition without adverse reactions.

ObjectiveTo investigate the effect of high volume hemofiltration (HVHF) combined with mechanical ventilation (MV) on seawater respiratory distress syndrome (SW-RDS) canine models.MethodsTen nomal hybrid dogs were randomly assigned into two groups:MV group(MV group,n=5),all the animals only received MV after establishing model successfully; HVHF combined with MV group (HVHF+MV group,n=5),all were received HVHF plus MV after establishing model successfully.Both groups were observed for 4 hours.Mean arterial pressure (MAP),heart rate (HR),central venous pressure(CVP),arterial blood gas and venous plasma osmotic pressure were detected at baseline,0 min(model establishment),60 min,120 min,180 min,240min after treatment.Venous blood was collected to detect inflammatory mediators (IL-8,IL-6,TNF-α)at baseline,0 min,120 min,240 min after treatment.The lung pathology was examined at the end of the experiment.Results(1)All the animals were suvival after four hous of treatment in both groups. (2)Pattial pressure ofoxygen(PaO2) and O2 saturation(SaO2) rised after four hours of treatment in both groups(P＜0.05), and HVHF+MV group was better than MV group.After 4 hours of treatment,pH,actual bicarbonate (AB),bases excess(BE) in HVHF+MV group were significantly better than those in MV group(P＜0.05),recovering to the baseline values.(3)MAP,HR,CVP were stable during the four hours of treatment,and compared with 0 min,there was no significant differences after 4 hours of treatment in bothgroups. There were no significant differemces at the same time of treatment in both groups. (4)Plasma osmotic pressure were stable during the four hours of treatment,and compared with 0 min,there was no significant difference in MV group.But in HVHF+MV group,osmotic pressure was significantly higher after 4 hours of treatment than that at the same time in MV group(P＜0.05),and compared with 0 min and 180 min,those were higher too(P＜0.01). (5)Compared with those at the same time in MV group,plasma inflammatory mediators (IL-8,IL-6,TNF-α) were significantly decreased after 4 hours of treatment in HVHF+MV group(P＜0.01).After 4 hours of treatment IL-8,TNF-α in MV group were higher than those at the same time in 0 min (P＜0.05).(6)Compared with those in MV group,there were less infiltration of neutrophils,edema and injury of alveolar epithelium from pulmonary pathology.ConclusionsHVHF combined with MV can significantly improve hypoxemia and correct acidosis of SW-RDS model in canines.HVHF can effectively clear plasma inflammatory mediators and redundant water,improve pulmonary pathology changes.HVHF has no impact on MAP,HR and CVP of SW-RDS.

Objective To investigate and analyze the postoperative progression of ossification of the patients with ossification of the posterior longitudinal ligament (OPLL) on cervical spine. Methods From Jaunary 2001 to December 2007, 95 postoperative patients with cervical OPLL were followed and analyzed retrospectively. There are 72 males, 23 females, with the average age of 56.3 years (range, 40-73years). The follow-up time was from 1 to 6 years, average 3.1 years. Among them 36 patients were performed with anterior cervical corpectomy, fusion with titanium mesh and fixed with cervical plates, others treated with posterior cervical laminectomy and fixation. 2 of the 95 cases were performed anterior and posterior combined operation. Clinical data, X-rays, CT and MR images and progression of ossification, were measured and analyzed in details .The relationships between the progression of ossification and relative factors, as gender, age, C3 ossified involved, T-OPLL, OPLL-type, time of follow-up, surgical approach, Japanese Orthopaedic Association (JOA) scores and improvement rate of JOA scores, were analyzed. Results Progression of ossification in 39 cases among the 95 followed postoperative OPLL patients, 28 men and 11 women, average age 55.9 years, range 41-71 years. The age of progressed patients included 12 cases of ≤49 years, 12of 50-59 years, 12 of 60-69 years and 3 of ≥70 years. 35 patients were operated by posterior approach and only 4 treated with anterior operation. According to the standard of the progression of ossification that 2 mm in the length or/and thickness, there are 4 cases progressed only in length, 2 only in thickness, other 33 patients both the length and thickness. Progression of length is from 2mm to 20mm (average 7.74±4.71). But thickness is progressed from 2 mm to 6 mm (average 2.67±1.51). From 1 to 3 years follow-up time it appeard as a downtrend about the progression of ossification. But it may appear an uptrend from the 4th year. JOA score and improvement rate of the JOA score were almost improved to the high-point in three years. And according to the statistic data there are obvious relationship between progression of ossification with age, surgical approach and C3 ossified involved. Conclusion There is a high rate of postoperative ossification progression in cervical OPLL patients. Cervical OPLL patients with C3 ossification involved, performed with posterior laminectomy and those young at surgery may have higher rate of progression of the ossification. The JOA score and improvement rate of the JOA score were little influenced by the progression of the OPLL during the short and intermediate-term follow-up.

Objective To explore the relationship between cough variant asthma (CVA) and mycoplasma pneumoniae (MP) infection.Methods Fifty children with CVA were chosen as the experimental group at random,and 50 children with acute upper respiratory infection,who went to the hospital in the same time and with similar age,were chosen as control group.The MP-IgM of children in both groups were tested by the granule agglutinating method.Results Significant difference (? 2=9.013 P

In the reform of medical microbiology education on medical students of 8-year-education pro-gram, we compared the traditional teaching methods with Problem Based Learning (PBL) method. Through our practice, we have found that the combination of traditional lecture-based learning and PBL seems to better match the students’ way of learning. The lack of basic knowledge of the students hindered their learn-ing effect during the bilateral discussion in the PBL education. We also found that the application of PBL in medical microbiology education is an iterative process and should be promoted step by step. The theoretical level and the innovative ideas of the teachers play a crucial role in the dynamic process of education reform.

Our study investigated the neurotoxicity of quinolinic acid (QA) to spiral ganglion cells (SGCs), observed the protective effects of N-methyl-D-aspartate (NMDA) receptor antagonist MK-801 and magnesium ions on the QA-induced injury to SGCs, and analyzed the role of QA in otitis media with effusion (OME)-induced sensorineural hearing loss (SNHL). After culture in vitro for 72 h, SGCs were exposed to different media and divided into 4 groups: the blank control group, the QA injury group, the MK-801 treatment group, and the MgCl(2) protection group. The apoptosis rate of SGCs was analyzed by Annexin V and PI double staining under the fluorescence microscopy 24 h later. SGCs were cultured in vitro for 72 h and divided into four groups: the low concentration QA group, the high concentration QA group, the MK-801 group, the MgCl(2) group. The transient changes of intracellular calcium concentration were observed by the laser scanning confocal microscopy. Apoptosis rate in QA injury group was higher than that in blank control group and MgCl(2) protection group (both P<0.05), but there was no significant difference between MK-801 treatment group and blank control group (P>0.05). In high concentration QA group, there was an obvious increase of the intracellular calcium concentration in SGCs, which didn't present in low concentration QA group. In MgCl(2) group, the peak values of the intracellular calcium concentration in SGCs were reduced and the duration was shortened, but the intracellular calcium concentration in SGCs had no significant change in MK-801 group. It was concluded that QA could injure SGCs by excessively activating NMDA receptors on the cell membrane, which might be the mechanism by which OME induced SNHL, while Mg(2+) could protect the SCGs from the neurotoxicity of QA.

Objective To study the biological effects of cathepsin B phosporotbioated antisense oligodeoxynucleotide on human osteosarcoma cell line MG-63 after transfection. Methods A 18-mer phosphorothioate antisense oligodeoxynucleotide (ASODN) targeted against the cathepsin B mRNA was transfected into the human osteosarcoma cell line MG-63 by lipofectamine 2000. The sense and nonsense oligodeoxynucleotides to cathepsin B and blank vector were used as controls. The expression of cathepsin B mRNA was examined by RT-PCR and the expression of cathepsin B was examined by Western blot. The invasive capability of MG-63 cells was evaluated by the boydern chamber assay. Results The expression of cathcpsin B was obviously inhibited in antlsense oligodeoxynucleotide treated cells compared with the control cells. The number of invading MG-63 cells was significantly lower in the ASODN-treated groups than that in the control groups. Conclusion The cathepsin B ASODN significantly inhibits the expression of cathepsin B and invasive ability of MG-63 cell in osteosarcoma.