OBJECTIVETo detect the histological changes and the expression of proliferating cell nuclear antigen (PCNA) in junctional epithelialization of dental implants.

METHODSThe junctional epithelium was detected by HE staining and the PCNA expression was observed by immunohistochemical staining on the st, 3rd, 6th, 9th, 12th, 15th, 20th and 30th day after implantation in Beagle dogs.

RESULTSThe junctional epithelium began to form and PCNA was expressed positively 12 days after implantation. The epithelial hyperplasia tended to stabilization and the expression of PCNA weakened drastically 15 days after implantation. The epithelial attachment showed to be similar to that of natural teeth 20 days later.

CONCLUSIONThe gingiva epithelium can attach to titanium implants forming junctional epithelium which is similar to the natural teeth.

Animals ; Dental Implantation ; Dental Implants ; Dogs ; Epithelial Attachment ; Female ; Male ; Proliferating Cell Nuclear Antigen ; biosynthesis ; Titanium