2.The study of genome DNA methylation in rheumatoid arthritis
Xiao-Yan XU ; Mei-Mei WANG ; Chuan-Shi XIAO ; Xiao-Feng LI ; Lai-Yuan WANG ;
Chinese Journal of Rheumatology 2003;0(08):-
Objective To study the genome DNA methylation in rheumatoid arthirits(RA)and the re- lated factors of DNA methylation.Methods Twenty-first cases with RA and 20 controls were recruited to par- ticipate the study.Plasma Hcy,SAM,SAH,the MTHFR gene C677T polymorphism and the expression of LFA-1 in CD4~+T cells was measured in all patients and controls.Results①The SAM levels were lower sig- nificantly in RA groups than in controls.The SAH levels were higher significantly in RA groups than in con- trols.②There was significant inverse correlation between plasma Hcy level and SAM level(r=-0.932,P<0.01). There was significant positive correlation between plasma Hcy level and SAH level(r=0.924,P<0.01).③The expression of LFA-1 in CD4~+T cells was higher significantly in RA groups than in controls.There was a signif- icant positive correlation between LFA-1 expression level and Hcy level(r=0.557,P<0.01),a significant in- verse correlation between LFA-1 expression level and SAM level(r=-0.651,P<0.01).④The MTHFR gene mu- tation lead to dramatically increase of Hcy,SAH level and the expression of LFA-1 level in CD4~+T cells and genome DNA hypomethylation.Conclusion①Hypomethylation of genome DNA is found in most RA pa- tients.②The factors associated with genome DNA hypomethylation include MTHFR gene mutation and hyper- homocysteinemia.③The expression of LFA-1 in CD4~+ T cells is higer in RA groups than in controls,which re- lates to the DNA methylation level and the MTHFR gene C677T polymorphism.
3.The Progress of Biological Research of Medicinal Fungus Shiraia bambusicola
Xiao-Ming JIA ; Xiao-Hong XU ; Bai-Chuan ZHUANG ; Hai-Ping LIN ;
Microbiology 1992;0(03):-
The research status is reviewed on taxonomic position, ecology, biological active matter and artificial cultivation of Shiraia bambusicola. Following problems are presented: whether there are new species and host specificity of Shiraia bambusicola. Since the artificial cultivation of stroma of Shiraia bambusicola has not been success, Shiraia bambusicola is in situation of emerge of itself and perish of itself so that it is not beneficial to resource protection and continual utilization of this medicinal fungus. It is pointed out that molecular biology of Shiraia bambusicola should be further researched and technology of artificial cultivation of Shiraia bambusicola should be explored in order to exploit and usage of Shiraia bambusicola.
4.Comparison of Pattern-pulse and Pattern-reversal Multifocal Visual Evoked Potentials in Normal Individuals.
Rong-ting ZHU ; Xing-ben LIU ; Xiao-ming XU ; Chuan-fei ZHENG
Journal of Forensic Medicine 2016;32(1):35-39
OBJECTIVE:
To compare pattern-pulse multifocal visual evoked potential (PPmfVEP) with pattern-reversal multifocal visual evoked potential (PRmfVEP), and to investigate the symmetry of mfVEP between both eyes in normal individuals.
METHODS:
The multifocal Vision Monitor was used to observe the mfVEP. T-test and ANOVA were used to analyze P1 wave, amplitude and signal noise ratios (SNR) of two mfVEPs.
RESULTS:
The SNR and the P1 amplitude reached the maximum at the central visual field and decreased with the increase of eccentricity, and then decreased slowly. The amplitude of the PPmfVEP was significantly smaller than the PRmfVEP in the central retina, while in the peripheral retina the result was exactly the opposite. SNR and amplitude of the PRmfVEP showed no statistical difference in both eyes (P > 0.05). The variance of the amplitude at the same side of visual field was larger than that at the symmetrical visual quadrant.
CONCLUSION
mfVEP can reflect the visual function in different parts of retina objectively and exactly. PPmfVEP reflect the vision function of the central retina better than PRmfVEP. The stability of PPmfVEP is better than PRmfVEP in the central retina, while the result is opposite in the peripheral retina. The mfVEP is symmetrical in both eyes of the same individual.
Evoked Potentials, Visual/physiology*
;
Humans
;
Neurologic Examination
;
Reference Values
;
Retina
;
Sensitivity and Specificity
;
Visual Fields/physiology*
5.Clinical significance of detection of tumor suppressor genes aberrant methylation in cervical carcinoma tissue
Jun XU ; Hong-Lin WANG ; Gao-Chuan LU ; Zhi-Jie WANG ; Xiao LIN ; Hong-Wei ZHOU ;
Chinese Journal of Obstetrics and Gynecology 2001;0(06):-
0.05).(4) Significant differences between CC and CIN Ⅰ for p16,CDH1,RASSF1A and TIMP3 genes(P
6.Accuracy for cytological evaluation in the detection of breast cancer among patients with pathologic nipple discharge: a PRISMA-compliant meta-analysis
Li XIAO-QIAN ; Xu FENG ; Lei CHU-QI ; Li JIE ; Jiang HONG-CHUAN
Chinese Medical Journal 2020;133(4):435-443
Background:Nipple discharge cytology is a simple non-invasive method that may provide valuable information for detecting underlying malignancy.Several studies have investigated the diagnostic value of cytology in breast cancer patients with pathological nipple discharge,but the results have been highly variable.Herein we presented a systematic review and meta-analysis of published studies pertaining to the diagnostic capacity of nipple discharge cytology in patients with breast cancer.Methods:A systematic literature search was performed (Medline/PubMed,Embase,Cochrane Library databases,and Google Scholar) to identify studies that investigated the diagnostic capacity of cytology with regard to breast cancer in patients with pathologic nipple discharge.Two independent researchers identified articles that assessed the sensitivity and specificity of cytological evaluation for breast cancer detection in patients with pathologic nipple discharge published between January 2000 and October 2018.Articles were only included in the meta-analysis if they met predetermined criteria.The characteristics of each study and the data they yielded were summarized.Quality assessment of all articles included was performed using the Methodological Index for Non-randomized Studies Criteria (MINORS) and the Quality Assessment of Diagnostic Accuracy Study 2 (QUADAS-2).Heterogeneity was tested via Cochran Q test and the I2 statistic using Stata 12.0 and Meta-DiSc 1.4 software,and meta-analysis was performed.Results:A total of 286 articles were identified,of which 12 articles including a total of 1476 patients were deemed eligible for inclusion in the meta-analysis.A random-effects model assessing the capacity of nipple discharge cytology to predict breast cancer yielded pooled sensitivity 63% (95% confidence interval [CI]:53%-72%),specificity 95% (95% CI:87%-98%),positive likelihood ratio 12.35 (95 % CI:4.87-31.34),and negative likelihood ratio 0.39 (95 % CI:0.30-0.50).The diagnostic odds ratio was 31.88 (95% CI:11.30-89.98).The area under the summary receiver operating characteristic curve was 0.79 (95% CI:0.75-0.82).Conclusion:The current meta-analysis suggests that nipple discharge cytology is a useful diagnostic modality for detection of breast cancer in patients with pathological nipple discharge,with moderate sensitivity and high specificity.
7.Clinical Study on Serum Vascular Endothelial Growth Factor in Human Colorectal Carcinoma
De-Chuan LI ; Hai-Yang FEN ; Xiao-Hong XU
Chinese Journal of Cancer 2001;20(3):314-316
Objective:This study was designed to determine the serum vascular endothelial growth factor (VEGF)level in human colorectal carcinoma and analyze its clinical significance. Methods:The level of serum VEGF was measured by ELISA kit (R& D,SYSTEM INC,USA) in 82 patients with colorectal carcinoma and 30 normal persons as the control group. Results:The level of serum VEGF in colorectal carcinoma was significantly higher than that of normal control. The level of serum VEGF was positively correlated with tumor size, vessel invasion, lymph-node metastasis. With upgrading of clinical-pathological stage(Dukes’stage), the level of VEGF increased significantly, but its relation to tumor site, histological type, age, and sex was not significant. Conclusion:The level of serum VEGF is closely correlated to invasion and metastasis of colorectal carcinoma. Serum VEGF assay may be used to preoperatively predict invasion and metastasis of colorectal carcinoma.
8.Detection of growth phenotype and blood biochemical parameters of wild type-derived chromosome 1 substitution mouse strain
Chuan GAO ; Wei XU ; Fuyi XU ; Yaoqi ZHANG ; Ying ZHAO ; Liya ZHAO ; Yuxun ZHOU ; Kai LI ; Junhua XIAO
Acta Laboratorium Animalis Scientia Sinica 2015;(3):233-238
Objective To analyze the growth phenotype and blood biochemical parameters of chromosome 1 substi-tution mouse strain(CSS1), and investigate their potential of QTL mapping .Methods Body weight, body length, tail length, organ weight of the CCS1 mice were measured at different days to create a growth curve while blood biochemical in -dexes were measured at about the 80th day.Results The CCS1 mice were different from C57BL/6 mice in several inde-xes.Compared with the C57BL/6 mice during different developmental stages , six strains including B6-Chr1KM mice were significantly different in body weight .There were five strains including B6-Chr1CM mice significantly different with C57BL/6 mice in body length, and all of the CSS1 mice were significantly different from C57BL/6 mice in tail length.Part of CCS1 mice were significantly different from C57BL/6 mice in the weight of liver, spleen, kidney and brain.The ALT of female B6-Chr1CM mice was significantly higher than that in the C 57BL/6 mice.The ALP of female B6-Chr1HZ mice was signifi-cantly higher than that in the male C57BL/6 and B6-Chr1KM mice, and was significantly lower than that in the C57BL/6 mice.The TB of male B6-Chr1CM, B6-Chr1SMX and B6-Chr1HZ mice was significantly higher than that of the C 57BL/6 mice.The TG of male B6-Chr1SMX mice and male B6-Chr1TW mice was significantly higher than that in the C 57BL/6 mice. Conclusions The phenotype of Chr1 CSS mice is quite different from commonly used inbred strain C 57BL/6 mice.CCS1 mice show great potential in QTL mapping for their characteristic growth phenotype and blood biochemical indexes .
9.Permeability research of human temporomandibular joint disc and cartilage.
Xiao-chuan XU ; Meng-shi CHEN ; Xin-zhu YI
West China Journal of Stomatology 2006;24(3):254-256
OBJECTIVETo measure the permeability of human temporomandibular joint (TMJ) disc and cartilage to provide basic parameter for oral biomechanics and tissue engineering, and analyze its mechanisms of pathology and load-release.
METHODSConfined compression method was used to measure the permeability (k value) of four cadavers' TMJs, which were sampled into three parts: disc, condyle and glenoid fossa with different diameters (2 mm, 3 mm and 4 mm). All 128 samples were tested with correspond diameter indenter.
RESULTSLarger the sample diameter was, higher the k value became. The highest k value appeared in the disc while the lowest appeared in glenoid fossa.
CONCLUSIONIn normal condition, TMJ can suffer huge load by decreasing its permeability. Disc is weakest for the higher permeability, it's easy-damaged region is an initiated factor of TMJ disease.
Cartilage ; Humans ; Permeability ; Temporomandibular Joint Disc ; Tissue Engineering
10.Analysis of current γ knife stability testing status in medical institutions
HU Chuan peng HUANG Wei xu ZHAI He zheng CHENG Xiao jun ZHANG Lei JIA Chen zhi
China Occupational Medicine 2022;52(04):464-
Abstract: Objective
To follow up and evaluate the stability testing status of γ stereotactic radiotherapy system (hereinafter
γ knife WS 582-2017 Specifications for Testing of Quality Control in X and γ
referred to ) in medical institutions based on the
RayStereotactic Radiotherapy System WS 582-2017 Methods
(hereinafter referred to ). A total of 43 medical institutions using
γ knife and 54 medical physics personnel were recruited as the research subjects by random sampling method. Based on the
- -
standard tracking evaluation method, we followed up the evaluation of γ knife stability testing based on WS 582 2017.
Results -
Only 66.7% of the 54 subjects preformed WS 582 2017 in their daily work. Only 30.2% (all were tertiary hospital) of
43 medical institutions were equipped with complete quality control and testing equipment. The implementation rate of γ knife
stability test was 67.4%, and it was not carried out in strict accordance with the test events and test cycle requirements
-
specified in the WS 582 2017. There were incomplete test events, incomplete records or test cycles that did not meet the
Conclusion
standard requirements in γ knife stability test. Medical institutions did not carry out γ knife stability tests in strict
-
accordance with the projects and cycle required by the WS 582 2017. The reasons are related to medical institutions are not
equipped with complete testing equipment, the personnel are lack of understanding of relevant national standards, the poor
publicity and implementation of the standards, the lack of standardized training channels and inadequate health supervision.