Humans ; Occupational Health ; Polymorphism, Single Nucleotide

Objective To study the effects of valproic acid (VPA) to the expression of SMN2 mRNA in neuron-like cells (NLCs) derived from patients with spinal muscular atrophy (SMA). Methods Polymerase chain reaction-restriction fragment length polymerphism (PCR-RFLP) was performed to select the patients with SMA. Mesenchymal stem cells (MSCs) derived from patients were induced into NLCs which were set as the model of neurons. The transcripts of SMN2 by reverse transcription-polymerase chain reaction (RT-PCR) combined with sequenceing were detected. Semi-quantitative RT-PCR analysis was performed to detect the changes of SMN2 mRNA expression between before and after the NLCs were treated by VPA. Results Two bands (266 bp and 212 bp) were found in the gel picture of RT-PCR, which were respectively the products of full length transcript (fl-SMN mRNA) and skipping exon 7 (SMN?7 mRNA). NLCs had significantly increased fl-SMN mRNA and SMN?7 mRNA levels as compared with the untreated cells after treatment with VPA, and shown a dose effect(0.210?0.035,0.282?0.041,0.351?0.020,0.450?0.052,0.553?0.035,P

Hedgehog family proteins are important morphogens which mediate the development of embryo as well as the carcinogenesis in adults. Inappropriate activation of Hedgehog signaling pathway contributes to numerous human cancers. The Hedgehog signaling pathway is considered to be involved in the molecular mechanism of the invasion and metastasis of the tumors, and to facilitate the metastasis of tumors through signaling pathways interaction. It is suggested that a potential therapeutic strategy pharmacologically targeting Hedgehog-dependent tumors may be developed.

BACKGROUND:Anterior cruciate ligament is the important anatomic structure to maintain the knee joint stability. The tendon bone healing and clinical functional recovery after anterior cruciate ligament have attracted more attention. OBJECTIVE:To observe the healing of graft tendon and surrounding bone with histological method through the same diameter grafts matching with the bone tunnel of different sizes in the anterior cruciate ligament reconstruction surgery, and to detect the functional recovery with biomechanics. METHODS:Middle 1/3 of canine autologous tendon was selected as the anterior cruciate ligament graft, and then trimmed into the same diameter of 4 mm. Sixteen adult mongrel canine were randomly divided into four groups. The anterior cruciate ligament was resected completely, and the tibial and femoral tunnels were prepared on the end sites of tibia and femur with the diameters of 5, 4.5, 4 and 3.5 mm, then implanted into the tendon in prepared and linked into the bone tunnel. At 6 weeks after reconstruction, the experimental canine were sacrificed under general anesthesia to col ect the tissue and organs in the surgical area. Then the hematoxylin-eosin staining, biomechanical testing and statistical analysis were performed. RESULTS AND CONCLUSION:At 6 weeks after anterior cruciate ligament reconstruction, anatomical observation showed that there were no significant differences in growth of grafts and bone tunnels between groups;hematoxylin-eosin staining showed sharpey-like fibronectin could be seen in the tendon bone healing surface, and the col agen fibers in the 3.5 mm bone tunnel group were more compact and regular than those in the other groups;the biomechanical testing results in the 3.5 mm bone tunnel group were better than those in the other groups. The results indicate that during anterior cruciate ligament reconstruction, decreasing the diameter of bone tunnel that matched with grafts in order to make the tendon and the bone tunnel closely matched can provide a more stable cel biological and mechanical environment, accelerate the formation and transformation of tendon-bone healing interface, and can improve the quality of tendon-bone healing.

Hepatocellular carcinoma(HCC)has high incidence rate,recurrence rate after surgecal treat-ment,as well as fatality rate in China .HCC is not sensitive to radiotherapy or chemotherapy and no effective treat-ment is available for HCC patients at advanced stage .Sorafenib is the first effective molecularly targeted drug for the treatment of HCC,which represents a milestone in the treatment of HCC .However,it also shows drug resist-ance during clinical application .Therefore,it is important to investigate the mechanism of drug resistance and its molecular markers for HCC .In this review ,we summarize the current status of the studies in these fields .

Objective To study the clinical and pathological manifestation of simple polymyositis. To investigate the expression of costimulatory molecules CD28/CTLA-4: B7 in peripheral blood lymphocytes and their roles in the pathogenesis of SPM.Methods The clinical situation, serum emzymes, electromyography(EMG) and muscular pathology of 141 patients with SPM were investigated. The expression of costimulatory molecules CD28, CTLA-4, B7-1, BB-1 and B7-2 in peripheral blood lymphocytes of six patients with simple polymyositis was measured with one-color flow cytometry(FCM). The control group were healthy volunteers.Results Muscle weakless, myalgia, elevation of creatine kinase and abnormal EMG of myogenic damage were very frequently to see in SPM. The muscle biopsy showed degeneration, necrosis and regeneration of muscle fibres, sporadic muscle fibre atrophy and endomysial inflammatory infiltration. The expression levels of costimulatory molecules CD28, CTLA-4, B7-1 and B7-2 in peripheral blood lymphocytes increased in SPM. Compared to control group, the mean fluorescence intensity of these molecules in SPM group showed by FCM increased remarkably (CD28, B7-2, P

Objective To explore the expression of G protein inwardly rectifier potassium channels subunit 2(GIRK*!2) in hippocampus of temporal epileptic rat.Methods After temporal lobe epilepsy was induced by kainic acid (KA), we took advantage of in situ hybridization to investigate the altered expression of GIRK*!2 mRNA in rat hippocampus.Results GIRK*!2 mRNA significantly increased in epileptic rats dentate gyrus region compared with normal control( P

Objective To systemically discuss the role of Parvalbumin (PV), Calretinin (CR) and Calbindin-D28k (CB)-containing GABAergic interneurons in the acute onset and development of temporal lobe epilepsy.Methods Immunohistochemistry method was used to detect the changes of PV, CR and CB-containing interneuron numbers in hippocampus of temporal lobe epileptic rats induced by lithium-pilocarpine at different time points (6 h, 24 h, 7 d, 15 d, 30 d and 60 d).Results Compared with control group, no loss of PV-positive cells was observed in CA3 region at any time point in epileptic model groups, while dramatic reduction of PV-positive cells was seen in CA1 region ( P0.05). In CA1 region, the number of CB positive interneurons decreased dramatically at 6 h ( P

Objective To illuminate relationships between epilepsy and functional and morphologic plasticity of synapse through investigating temporal-spatial expression of syanpsinⅠand the alteration of synaptic ultrastructure in hippocampus after seizure. Methods The models of epilepsy were established by injection of pilocarpine and lithium. Electromicroscope and the software of image manipulation were applied to observe the alteration of synaptic ultrastructure in hippocampus during acute phase, resting phase and chronic phase. The expressions of synapsinⅠ were determined by immunohistochemistry. Results The expression of synapsinⅠin every subfield of hippocampus decreased at 3 h after induction of seizure, reached the peak at 6 h and 12 h, which was significantly different from the control ( P

Objective To investigate which subfamily genes of T cell receptor (TCR) V? expand predominantly during the course of experimental allergic neuritis (EAN). Methods Using RT-PCR and in situ hybridization techniques,the expression levels of TCR V? 2,6,8,10,14 in the peripheral blood,lymph nodes,peripheral nerves of group EAN and those of the control group were compared. Results In group EAN,the expression of TCR V? 6?8 mRNA increased at the early phage(41.1?1.1 and 74.4?1.9 vs 25.9?1.5 and 26.1?1.6) and became more significantly at the peak of the disease,and resolved to normal at the recovery phage in the lymph nodes.But they amounted up gradually from the early stage to the peak,and then decreased a little in the infiltrating T-lymphocytes in peripheral nerves,the difference was significant.In addition,the expression of TCR V? 8 was notably higher than that of TCR V? 6 in the levels of mRNA. Conclusion The subfamilies of TCRV? genes which restrict the development of EAN due to recognizing the specific antigens are TCRV? 8 and TCRV? 6. T lymphocytes specifically expressing TCR V?6?8 genes are activated and clonally proliferated in the lymph nodes,and then migrate to the involved peripheral nerves,which might induce a series of immune lesions consequently.