S100A8 and S100A9 are abundantly expressed in neutrophils cytoplasm,they are calciumbinding proteins and they often exist as S100A8/A9 heterodimer.Previous studies have shown that the biological functions of S100A8 and S100A9 are associated with chronic inflammatory diseases and a variety of cancers.They are important to inflammation by binding and activation Toll-like receptor4 (TLR4) and receptor for advanced glycation end products(RAGE),and mediating intracellular inflammatory signaling transduction.This review summarizes the studies on functions and molecular mechanism of S100A8 and S100A9 in autoimmune diseases,which might propose new strategies for diagnosis,treatment and suggested disease activity.

Objective To evaluate the feasible of dual-energy coronary artery CTA combined with affirmed iterative reconstruction technique for overweight patients.Methods 60 cases underwent dual-energy coronary artery CTA were enrolled in this reasearch.Patients were divided into two groups according to different reconstruction arithmetic model:group A with FBP reconstruction model,while group B with SAFIRE reconstruction model and monoenergetic images from 65 to 90 keV (with increment of 5 keV).All the data were measured by one doctor worked in CT department for more than 5 years,and the image quality were analyzed and evaluated by two senior radiologists.Randomized block ANOVA analysis was used to compare objective evaluation indices of different monoenergetic images from 65 to 90 keV to get the best keV level.The comparative t-test was applied to objective evaluation indices(CT values,SD,SNR,CNR and image quality score) of group A and B and comparative x2-test was applied to image quality score at the best keV level.Results There were significant differences in CT value,SD,SNR,CNR of different keV images (P ＜0.05).For objective evaluation of image quality,the SNR and CNR of group B were significantly higher than group A,and SD was significantly lower than group A (P＜0.05).For subjective evaluation of image quality,there were statistically significant difference between group B and group A (P＜0.05),the segment score of group B was superior to group A.Radiation dose:the DLP value was (238.7±30.82) mGy · cm,and the ED value was (3.3±0.43) mSv.Conclusion SAFIRE combine with purified single spectrum technique can improve the image quality and reduce radiation dose in dual-energy coronary artery CTA,and 75 keV is the best single energy level.

Humans ; Macrophage Activation Syndrome ; diagnosis

AIM: To explore the anti-LPS mechanisms of ?-melanocyte-stimulating hormone (?-MSH), the effects of ?-MSH on the expression of SOCS-3 mRNA and the production of NO in murine peritoneal macrophages induced by LPS were investigated. METHODS: BALB/c mouse peritoneal macrophages were cultured in vitro and induced by LPS, ?-MSH and LPS with ?-MSH, respectively. The expression of SOCS-3 mRNA was detected using reverse transcription polymerase chain reaction (RT-PCR). NO produced in macrophages was tested with Griess reagent. RESULTS: The level of NO and the expression of SOCS-3 mRNA were significantly increased in macrophages stimulated with LPS.?-MSH markedly decreased the expression of SOCS-3 mRNA and almost completely inhibited the production of NO induced by LPS. CONCLUSION: These results suggest that the negative regulative circuits operated by SOCS are activated during the inflammation induced by LPS, but SOCS might not be involved in the anti-LPS mechanism of ?-MSH.

Objective Liver is a common site of metastasis of gastric cancer and hitherto there has been no uniform treatment for liver metastasis of gastric cancer. This study was to investigate the clinical characteristics and prognostic factors of liver metastasis of gastric cancer in order to provide some evidence for the improvement of the diagnosis and treatment of the disease. Methods We ret-rospectively analyzed the clinical characteristics and survival data of 143 patients with liver metastasis of gastric cancer treated in our hospital between March 2007 and September 2012. We investigated the independent risk factors affecting the prognosis of the disease u-sing the Cox Regression Model. Results Seventy-five ( 52. 4%) of the 143 patients survived for 12 months, 41 ( 28. 7%) for 24 months, and 18 (12.6%) for 36 months, with a median survival time of 14 months. Univariate analysis revealed statistically significant differences in the survival time among the patients with different histological grades of primary gastric cancer, time of liver metastasis, types of metastatic liver cancer, extra-liver metastasis, and treatment methods ( P<0.05) . Multivariate Cox regression analysis showed the histological grade of gastric cancer to be low-or non-differentiation ( HR=2.67, 95% CI:1.51-4.72) and the type of liver meta-static cancer to be H2 ( HR=1.89, 95% CI:1.08-3.29) or H3 ( HR=2.13, 95% CI:1.32-3.44) , which were independent risk fac-tors affecting the prognosis of liver metastasis of gastric cancer, while palliative chemotherapy ( HR=0.55, 95% CI:0.32-0.95) was an independent protective factor of its prognosis. Conclusion Patients with liver metastasis from gastric cancer usually have a poor prognosis, especially those with histologically low-grade gastric cancer or type-H2 or -H3 liver metastatic cancer. The prognosis of thepatients undergoing palliative chemotherapy may have a better prognosis than those receiving best supportive care. A comprehensive therapy should be recommended to patients with liver metastasis from gastric cancer.

Objective To study the effect of metoprolol combined with benazepril on cardiac structure and heart function in patients with heart failure and the treatment of hypertension in the elderly. Methods 120 cases of elderly hypertensive patients with heart failure from June 2011 to June 2015 were studied, according to the random number table method, they were randomly divided into combination group and control group.The two groups were given symptomatic and supportive therapy, the control group on the basis of conventional treatment were given antihypertensive drugs of benazepril; combination treatment group were given metoprolol on the basis of control group. After treatment, the clinical efficacy of the two groups were evaluated, the changes of cardiac structure in the two groups were recorded and analyzed before and after treatment, and the blood pressure and cardiac function were analyzed. Results The total effective rate of the combined group was 91.67%, the control group was 78.33%, there was significant difference between the two groups (χ2=4.1830, P=0.0408). After 6 months of treatment, the blood pressure, cardiac structure and 6min walking distance were significantly improved in the two groups (P<0.05). The above indicators in combination group were significantly better than those in the control group (P<0.05). The complication rate in combination group was 8.33%, 13.33% in the control group, there was no statistically significant between two groups (χ2=0.7764, P=0.3782). Conclusion Metoprolol combined with antihypertensive treatment can significantly improve the clinical efficacy of the treatment, improve the cardiac structure and function.

Clinical risk index for babies( CRIB) is applied in low birth weight preterm neonates to assess the initial severity of illness,predict mortality risk rates,evaluate their own performance,and audit the performance between different medical institutions.CRIB plays an important role in the progressive develop-ment of neonatology.The scoring rules and advantagse of CRIB scorni g system were introud ced in this arti-cle.Teh scoring system is able to correctly predict mortaliyt probabilities and long-term neurodevelopmental outcomes for low birth weight preterm infants.

Objective To evaluate the reliability of using imipenem,meropenem,cefoperazonesulbactam,piperacillin-tazobactam in the treatment of hospital-acquired Gram-negative bacterial infections with Monte Carlo simulation(MCS).Methods The MIC of the four agents collected from hospital-acquired infections were detected in accordance with broth dilution method of Clinical and Laboratory Standard Institute (CLSI).MCS were conducted with MICs and the pharmacokinetics parameters of the four agents based on conventional dose regimens.The cumulative fraction of response (CFR) of time over MIC target attainment in different dosing regimen were generated.Results A total of 2 541 strains,including 2 093 strains of Enterobacteriaceae and 448 strains of glucose non-fermentative bacilli were collected.The MIC90 of imipenem and meropenem against Enterobacteriaceae were less than 1 mg/L in general,whereas MICg0 of two agents with β-1actamase inhibitors was around 64 mg/L.As to glucose non-fermenting bacteria,MICs of all the four agents were very high,especially to Acinetobacter baumannii,which indicated MIC50 more than 32 mg/L.MCS revealed that carbapenems had significantly higher CFR than those with β-1actamase inhibitors.Imipenem and meropenem (1 g,q8 h) obtained CFRs of 74.69％ and 81.42％,respectively.The CFR of cefoperazone-sulbactam (2 g,q8 h) and piperacillin-tazobactam (4 g,q6 h) (both excluding β-1actamase inhibitors) were just 49.59％ and 27.66％ respectively,which increased after excluding A.baumannii in piperacillin-tazobactam.Conclusions The conventional dose regimens of imipenem and meropenem are reliable for the empiric therapy of Gram-negative hospital-acquired bacterial infections.Piperacillin-tazobactam is suggested to use with higher doses or prolonged infusion time to satisfy the time of drug concentration exceeded the MIC (T ＞ MIC) requirement.More clinical studies of cefoperazone-sulbactam should be conducted to optimize its regimen and guarantee its efficacy.

Objective To compare the therapeutic efficacy and adverse effects between transcather arte-rial chemoembolization(TACE) and comprehensive therapy in primary advanced heptocellular carcinoma(HCC).Methods 216 patients with primary HCC were arranged randomly into two groups:104 (group A) were treated with TACE alone,the rest(group B)were treated with TACE combined with intraperitoneal perfusion chemothera-py, Chinese herbs and immunotherapy.The therapeutic efficacy and adverse effects were analysed and compared between two groups.Results The CR + PR rates were 28.84% in group A and 34.82% in group B.The 4-year survival rates were 1.92% in group A and 3.87% in group B(P < 0.05).There were no severe side effects in both groups.Conclusions The short-term effective rate and 4-year survival rate in comprehensive treatment group were superior to the control group.Therefore, the comprehensive treatment is worthwhile to use in primary advanced hepatocellular carcinoma.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.