Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on regulatory T cells(CD4+CD25+ Foxp3+ T cell)and airway inflammation in asthmatic rats. Methods Asthmatic models were established in 28 rats with ovalbumin as sensitinogen,and four groups were randomly divided(n=7).1,25(OH)2D3 was orally administered 1 h before challenge in treatment group 1,dexamethasone was subcutaneously injected 1 h before challenge in treatment group 2,both 1,25(OH)2D3 and dexamethasone were given 1 h before challenge in treatment group 3,while no medicine was administered 1 h before challenge in group 4(asthmatic control group).The levels of IL-4 and IL-10 in bronchoalveolar lavage fluid(BALF) were detected,the total cell numbers were obtained and the eosinophil count was performed.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells were measured with flow cytometry,and the mRNA expression of IL-10 and Foxp3 in lung tissues was detected.Wistar rats without asthma were served as normal controls(n=7). Results There were significant differences between asthmatic control group and normal control group in each parameter.Compared with asthmatic control group,the total cell number and eosinophil count decreased,the level of IL-4 decreased and the level of IL-10 increased in BALF in treatment groups.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells significantly increased,and the mRNA expression of IL-10 and Foxp3 in lung tissues increased in treatment groups compared with asthmatic control group.There was no significant difference between treatment groups and normal control group in each parameter. Conclusion Administration of 1,25(OH)2D3 increases the proportion of Treg and expression of Foxp3 mRNA in peripheral blood and spleen in asthmatic rats.1,25(OH)2D3 has a regulatory effect on Treg,and may serve as a potential therapeutic approach against asthma.

Adolescent ; Cerebellar Cortex ; metabolism ; pathology ; Cerebellar Neoplasms ; metabolism ; pathology ; surgery ; Female ; Follow-Up Studies ; Ganglioneuroma ; metabolism ; pathology ; surgery ; Humans ; Neurofilament Proteins ; metabolism ; Synaptophysin ; metabolism

Brain Neoplasms ; pathology ; Cerebellar Neoplasms ; pathology ; Ganglioglioma ; pathology ; Ganglioneuroma ; pathology ; Humans ; Neoplasms, Neuroepithelial ; pathology

Objective To observe the therapeutic effect of ultrasonic microbubble combined with bevacizumab (Avastin) on choroidal neovascularization induced by photocoagulation in rabbits.Methods CNV was induced by photocoagulation with argon laser in 30 rabbits (60 eyes).All of the rabbits underwent fundus fluorecein angiography (FFA) 21 days after photocoagulation;6-8 hours later,3 rabbits were randomly chosen to be executed to having the immunohistochemical examination.Twenty-one days after photocoagulation,27 rabbits were divided randomly into 3 groups:bevacizumb,ultrasonic microbubble+bevacizumb,and control group;each group has 9 rabbits (18 eyes).The rabbits in control group had no interference treatment;while the rats in bevacizumb and ultrasonic microbubble+bevacizumb group underwent injection with bevacizumb or ultrasonic microbubble+bevacizumb respectively.FFA was performed on all of the rabbits 7,14,and 28 days after photocoagulation to observe the inhibition of CNV;immunofluorecence and Western blot were used to detect the expression of VEGF in retina and choroid.Twenty-eight days is the time point to determine the therapeutic efficacy.The expression of VEGF and the results of FFA were the sdandards of the judgement of therapeutic efficacy.Results Proliferaion of CNV to the retinal inner layer and the obvious leakage of fluoresein in the photocoagulation area indicated that the model of CNV was set up successfully.Twenty-eight days after injection,obvious fluorescent leakage was found in the control group,and the average fluorescent leakage in bevacizumab group differed much from the control group(t=16.2952,P<0.05);while the difference between ultrasonic microbubble+bevacizumb group and bevacizumab group was also significant (t=4.7955,P<0.05).At the same time point,the expression of VEGF in bevacizumab group detected by immunofluorecent assay and Western blot differed much from the control group (t=7.0327,9.2596;P<0.05),and the difference of VEGF between ultrasonic microbubble+bevacizumb group and bevacizumab group was significant (t=2.9724,17.1937;P<0.05).this experiment show that ultrasound combined bevacizumab intravitreal injection of the therapeutic effect of CNV superior to other groups(P<0.01).Conclusion Ultrasound microbubble combined with bevacizumab injection may improve the therapeutic effect on CNV by inhibiting the expression of VEGF.

ObJeetive To report a simple and efficient method to label two different types of retinal ganglion cells (RGCs) in mouse retina.Methods Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal nmicroscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglion cells (ipRGCs) using Brn3a and Melanopsin.Results The double staining results of whole mount retina showed that conventional RGCs and melanopsin immunopositive ipRGCs had a complementary distribution in mouse retina,these two subtypes of RGCs were predominantly present in the ganglion cell layer.The numbers of ipRGCs was just about 1％-2％ of conventional RGCs,and the axons of ipRGCs toward the direction of the optic disc,several dendrites toward the inner plaximem layer.Conclusion The immunofluorescence double staining of whole mount retina is a simple,stable and efficient method to label two different types of mouse RGCs.

Objective To study the possible mechanism of Bushenqiangdu recipe in treatment of ankylosing spondylitis(AS). Methods 30 out-patients were treated by Bushenqiangdu recipe for 6 months. The expression level of IL-18,IFN-?,IL-4 mRNA in outer-circumference blood of as patients and healthy volunteers were studied,as well as the change expression level of IL-18,IFN-?,IL-4 mRNA of as patients outer-circumference blood before and after treatment. Result The expression level of IL-18,IFN-?mRNA in AS patients PBMC group is higher prominently than control PBMC group(P

Objective To assess the clinical value of 18F -FDG PET/CT in the diagnosis of primary, suspected recurrent and metastatic cervical cancer.Methods The results of 67 women who were suspected suffering from cervical cancer had undergone Color dopplar ultrasound,HPV,TCT,some of them do CT,MRI and SCC -Ag examination from April 2013 to April 2015 were retrospectively analyzed.Their 18F -FDG PET/CT images with the other test results were compared.The presence or absence of tumor was confirmed by histopathology.Results In the 67 patients,22 cases were finally diagnosed as primary cervical tumors,and 22 cases of recurrent and metastatic cervical tumors.In all the patients with cervical cancer,18F -FDG PET/CT found a total of 41 cases,which had contributed to the diagnosis in 94.0%,But 3 cases had not been found.For the detection of primary cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 4.2%,For the detection of recurrent and metastatic cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 7.0%,and 2 cervical adenocarcinoma patients were not diagnosed by 18F -FDG PET/CT.Conclusion The study showed a significantly higher sensitivity of 18F -FDG PET/CT for the detection of primary,recurrent and metastatic cervical cancer than the other auxiliary examination.18F -FDG PET/CT for the diagnosis of cervical cancer can be worth popularization.

Purpose:Cochlear implant is a sort of device which is used to restore normal heating for the profoundly deaf by electrical stimulation of the auditory nerve.Simulation of multiple channels demonstrates that the number of channels has significant influence on sound effect.Method:This paper reviews the Continuous Interleaved Sampling (CIS) strategy for cochlear implants,and simulates it in PC.The audible sound of cochlear implanter is synthesized,and preliminary analysis of the speech identification rate of CIS strategy in different channels has also been done based on the simulation.Result:CIS strategy using 6 or 8 channels can achieve better sound effect .Conclusion:Simulation results show that CIS strategy with envelope extraction and high number of channels can produce satisfactory speech synthesis effect

We describe a 44-year old man who suffered an isolated external auditory canal granulocytic sarcoma in remission of acute myeloid leukemia. The patient confirmed acute promyelocytic leukemia three years ago and the disease was in remission after treatment. Two months ago he presented pain and hearing loss in the left ear and the symptom developed gradually. At otoscopic examination a tumoral lesion was noted in the external auditory canal and computerized tomography scan showed a mass in the left external acoustic meatus without bone erosion.
Adult ; Ear Canal ; diagnostic imaging ; pathology ; Hearing Loss ; Humans ; Leukemia, Promyelocytic, Acute ; Male ; Sarcoma, Myeloid ; diagnostic imaging ; pathology ; Tomography, X-Ray Computed

Objective A two-stage renovation has nowadays been considered as the optimal mode for the treatment of infected total hip joint.Extensive bone defects were often found during the subsequent operation,and they should be repaired by using allograft to attain stability of the implants.The purpose of present study is to investigate the efficacy and safety of employing bone allograft in repairing the femoral defect caused by post-operation infection of hip arthroplasty in the second stage operation.Method From Mar.2001 to Dec. 2006,a total of 20 patients(20 hips)with infected total hip arthroplasty were treated with bone allograft on the femoral side in the second operation.Impaction bone grafting technique was performed for 16 patients using bone allograft combined with cemented stems,and un-ce- mented prosthesis combined with bone strut allograft were used in the other 4 cases.A regular follow-up was done and the outcomes were evaluated using the Harris Hip Score System and radiography.Result All patients were followed-up clinically and radiographically for an average of 20.3 months(4-61 months).No re-infection was found at the last time of follow-up.The mean Harris Hip Score was in- creased from 34.2 before operation to 87.7 after operation.90% of the patients were satisfactory with the results.Conclusion The bone allograft can be used for reconstructing bone defects in the patients after infection of total hip arthroplasty,either with combined with ce- mented stems or non-cemented stems.It is an effective and safe way in dealing with femoral bone defect after infection of total hip arthro- plasty.