Brain Neoplasms ; pathology ; Cerebellar Neoplasms ; pathology ; Ganglioglioma ; pathology ; Ganglioneuroma ; pathology ; Humans ; Neoplasms, Neuroepithelial ; pathology

Adolescent ; Cerebellar Cortex ; metabolism ; pathology ; Cerebellar Neoplasms ; metabolism ; pathology ; surgery ; Female ; Follow-Up Studies ; Ganglioneuroma ; metabolism ; pathology ; surgery ; Humans ; Neurofilament Proteins ; metabolism ; Synaptophysin ; metabolism

Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on regulatory T cells(CD4+CD25+ Foxp3+ T cell)and airway inflammation in asthmatic rats. Methods Asthmatic models were established in 28 rats with ovalbumin as sensitinogen,and four groups were randomly divided(n=7).1,25(OH)2D3 was orally administered 1 h before challenge in treatment group 1,dexamethasone was subcutaneously injected 1 h before challenge in treatment group 2,both 1,25(OH)2D3 and dexamethasone were given 1 h before challenge in treatment group 3,while no medicine was administered 1 h before challenge in group 4(asthmatic control group).The levels of IL-4 and IL-10 in bronchoalveolar lavage fluid(BALF) were detected,the total cell numbers were obtained and the eosinophil count was performed.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells were measured with flow cytometry,and the mRNA expression of IL-10 and Foxp3 in lung tissues was detected.Wistar rats without asthma were served as normal controls(n=7). Results There were significant differences between asthmatic control group and normal control group in each parameter.Compared with asthmatic control group,the total cell number and eosinophil count decreased,the level of IL-4 decreased and the level of IL-10 increased in BALF in treatment groups.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells significantly increased,and the mRNA expression of IL-10 and Foxp3 in lung tissues increased in treatment groups compared with asthmatic control group.There was no significant difference between treatment groups and normal control group in each parameter. Conclusion Administration of 1,25(OH)2D3 increases the proportion of Treg and expression of Foxp3 mRNA in peripheral blood and spleen in asthmatic rats.1,25(OH)2D3 has a regulatory effect on Treg,and may serve as a potential therapeutic approach against asthma.

Objective To analyze the mutations in nucleotide sequences of transfusion transmitted virus(TTV) in neonatal infection.Methods Neonatal serum TTV-DNA was detected by a nested PCR technique.Fifteen Chinese neonates with positive TTV-DNA were diagnosed as TTV infection.ORF1 sequences of TTV-DNA from these neonates were determined.Results Homology of Chinese TTV(C01-C15) and Japanese TTV(N22)isolated ranged from 87.1%-97.7% at nucleotide level,but there were point mutations in Chinese TTV,such as GG→TT in locus 112 and 113,TTATC→CCTAT in locus 236-240.Conclusions Chinese and Japanese TTV isolated had the same genotype.Some gene mutations may increase the TTV pathogen,and result in neonatal hepatitis syndrome or hyperbilirubinemia.

Objective A two-stage renovation has nowadays been considered as the optimal mode for the treatment of infected total hip joint.Extensive bone defects were often found during the subsequent operation,and they should be repaired by using allograft to attain stability of the implants.The purpose of present study is to investigate the efficacy and safety of employing bone allograft in repairing the femoral defect caused by post-operation infection of hip arthroplasty in the second stage operation.Method From Mar.2001 to Dec. 2006,a total of 20 patients(20 hips)with infected total hip arthroplasty were treated with bone allograft on the femoral side in the second operation.Impaction bone grafting technique was performed for 16 patients using bone allograft combined with cemented stems,and un-ce- mented prosthesis combined with bone strut allograft were used in the other 4 cases.A regular follow-up was done and the outcomes were evaluated using the Harris Hip Score System and radiography.Result All patients were followed-up clinically and radiographically for an average of 20.3 months(4-61 months).No re-infection was found at the last time of follow-up.The mean Harris Hip Score was in- creased from 34.2 before operation to 87.7 after operation.90% of the patients were satisfactory with the results.Conclusion The bone allograft can be used for reconstructing bone defects in the patients after infection of total hip arthroplasty,either with combined with ce- mented stems or non-cemented stems.It is an effective and safe way in dealing with femoral bone defect after infection of total hip arthro- plasty.

ObJeetive To report a simple and efficient method to label two different types of retinal ganglion cells (RGCs) in mouse retina.Methods Eyeballs were harvested from normal adult C57 BL/6 J mouse,the retinas were isolated,four radial cuts were done,the retinas were pasted on the nitrocellulose membrane with the ganglion cell layer upturned.The immunofluorescence double staining and laser confocal nmicroscope was used to reveal conventional retinal ganglion cells and intrinsically photosensitive retinal ganglion cells (ipRGCs) using Brn3a and Melanopsin.Results The double staining results of whole mount retina showed that conventional RGCs and melanopsin immunopositive ipRGCs had a complementary distribution in mouse retina,these two subtypes of RGCs were predominantly present in the ganglion cell layer.The numbers of ipRGCs was just about 1％-2％ of conventional RGCs,and the axons of ipRGCs toward the direction of the optic disc,several dendrites toward the inner plaximem layer.Conclusion The immunofluorescence double staining of whole mount retina is a simple,stable and efficient method to label two different types of mouse RGCs.

Purpose:Cochlear implant is a sort of device which is used to restore normal heating for the profoundly deaf by electrical stimulation of the auditory nerve.Simulation of multiple channels demonstrates that the number of channels has significant influence on sound effect.Method:This paper reviews the Continuous Interleaved Sampling (CIS) strategy for cochlear implants,and simulates it in PC.The audible sound of cochlear implanter is synthesized,and preliminary analysis of the speech identification rate of CIS strategy in different channels has also been done based on the simulation.Result:CIS strategy using 6 or 8 channels can achieve better sound effect .Conclusion:Simulation results show that CIS strategy with envelope extraction and high number of channels can produce satisfactory speech synthesis effect

Objective To assess the clinical value of 18F -FDG PET/CT in the diagnosis of primary, suspected recurrent and metastatic cervical cancer.Methods The results of 67 women who were suspected suffering from cervical cancer had undergone Color dopplar ultrasound,HPV,TCT,some of them do CT,MRI and SCC -Ag examination from April 2013 to April 2015 were retrospectively analyzed.Their 18F -FDG PET/CT images with the other test results were compared.The presence or absence of tumor was confirmed by histopathology.Results In the 67 patients,22 cases were finally diagnosed as primary cervical tumors,and 22 cases of recurrent and metastatic cervical tumors.In all the patients with cervical cancer,18F -FDG PET/CT found a total of 41 cases,which had contributed to the diagnosis in 94.0%,But 3 cases had not been found.For the detection of primary cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 4.2%,For the detection of recurrent and metastatic cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 7.0%,and 2 cervical adenocarcinoma patients were not diagnosed by 18F -FDG PET/CT.Conclusion The study showed a significantly higher sensitivity of 18F -FDG PET/CT for the detection of primary,recurrent and metastatic cervical cancer than the other auxiliary examination.18F -FDG PET/CT for the diagnosis of cervical cancer can be worth popularization.

Objective To study the possible mechanism of Bushenqiangdu recipe in treatment of ankylosing spondylitis(AS). Methods 30 out-patients were treated by Bushenqiangdu recipe for 6 months. The expression level of IL-18,IFN-?,IL-4 mRNA in outer-circumference blood of as patients and healthy volunteers were studied,as well as the change expression level of IL-18,IFN-?,IL-4 mRNA of as patients outer-circumference blood before and after treatment. Result The expression level of IL-18,IFN-?mRNA in AS patients PBMC group is higher prominently than control PBMC group(P

Objective To explore the significance of ultrasound in choosing the delivery modality in 200 cases scarred uterus gravida′s second deliveries .Methods The study selected 200 cases late pregnancy gravidas who had once uterine incision delivery history and analyzed clinical data retrospectively ,all of them were chosen from September 2010 to September 2013 in our hospital . All cases were taken by transabdominal ultrasound or transvaginal ultrasound firstly ,then taken by transabdomina transvaginal ul‐trasound ,finally contrast obsenved the transabdomina transvaginal ultrasound detections with their caesarean section observations , meanwhile observe how the uterus scar ranking affect the delivery consequence .Results (1)A total of 200 cases had higher uterus scars visualization ratio(100 .0% ) than ransabdominal ultrasound (92 .0% )and transvaginal ultrasound(96 .5% )(P<0 .05);con‐trasting the transabdomina transvaginal ultrasound with the caesarean section observations ,there was no significant difference(P>0 .05) .The grading diagnosis coincidence of transabdomina‐transvaginal ultrasound was 89 .74% .(2)The UD rate ,the bleeding vol‐ume during operation and the 24 hours postoperative bleeding volume of scarred uterus gravida grade Ⅱ and grade Ⅲ were signifi‐cantly higher than grade Ⅰ (P<0 .05) .Conclusion Transabdomina transvaginal ultrasound can effectively improve the diagnosis coincidence rate of uterine scar detection and can help to chose the better delivery mode according to the scars healing .