Congresses as Topic ; Laryngeal Diseases ; Otolaryngology ; Pediatrics ; Pharyngeal Diseases

Objective To study the infection rate and sensitivity of genitourinary tract mycoplasma among the patients in the traditional Chinese medicine(TCM) hospital ,and its similarities and differences with the western medicine hospital ,thus to increase the therapeutic effect by combining with the related therapeutic measures of TCM .Methods The mycoplasma identification and drug susceptibility test results in 4 086 female genital tract specimens were performed the retrospective analysis .Then the medica‐tion strategy was investigated by combining with clinic .Results Of the 4 086 specimens ,1 891 cases were Mycoplasma positive with the total positive rate of 46 .3% ;in which the positive rate of ureaplasma urealyticum(UU) was higher(40 .7% ,1663 cases) , the most sensitive drugs were pyostacin and josamycin with the sensitive rates of 99 .11% and 99 .01% respectively ;mycoplasma hominis(M H) was less with the positive rate of 2 .7% (110 cases) ,josamycin and doxycycline were sensitive;the positive rate of UU and M H was 2 .9% (118 cases) ,doxycycline and pyostacin were sensitive with the sensitive rates of 95 .77% and 95 .26% re‐spectively .Conclusion Mycoplasma has higher infection rate in the genitourinary tract among gynecological patients ,the results are similar between the hospital of TCM and Western medicine hospital ;UU is mainly Mycoplasma type;the drug susceptibility test re‐sults reveal that the empirical medication for anti‐mycoplasma infection can select doxycycline and josamycin;it is recommended that the combined therapy with syndrome differentiation of traditional Chinese medicine by combining with the drug sensitivity test re‐sults has better clinical efficacy .

Objective To improve the nursing skills of low age and service seniority nurses and meet the needs of clinical nurses,in order to serve patients better and improve the quality of cave.Methods According to knowledge tests 50 points for nurse job-related skill issued by Chinese Ministry of Health,our hospital selected 12 commonly used basic operation skills and designed comprehensive cases through integration and reformation.We trained and examined nurses with undergraduate degree came to our hospistal in 2008.Results After training,the score of clinical nursing skill reached 91.81,86 nurses reached the standard and the effective compliance rate was 95%.Conclusions It proved to be an effective method to improve the nursing skills of low-age and service seniority nurses by carrying out the comprehensive casestyle training methods.

Objective To investigate the value of MR perfusion imaging in early detection of findings following arterial chemoembolization of hepatocellular carcinoma Methods Twenty eight consecutive patients with pathologically-confirmed HCC were evaluated. All patients underwent MR perfusion imaging at pre-TACE and 3 to 10 days after TACE. The negative enhancement integral (NEI) ,the time to peak(TTP) ,the maximum slope of decrease (MSD) , the signal enhance ratio (SER) were acquired from MRI software FuncTool 2. 5.36a Version. Statistical analysis using SPSS 14, least significant difference test (t test) were utilized. Results The time intensive curve of tumor was observed to descend rapidly to reach the peak at pre-TACE studies, whereas it descended slowly to reach the peak on post TACE studies. The Value of TTP and SER prior to TACE were(51.2 ± 10. 3) s, 60. 6 ± 36. 3 respectively, and post TACE (43.7 ± 12. 0)s, 41.2 ±27. 5 respectively. The values of TTP and SER post TACE were lower than those prior to TACE (P ＜ 0. 05). The value of NEI prior to TACE was 108.7 ± 58.9, and after TACE 149. 6 ±80. 1 and there was statistically significant difference (P ＜0. 05). The Value of MSD post TACE were lower than those prior to TACE, but there was no statistical significance (P ＞ 0. 05). Conclusion PWI is a very sensitive imaging technique that can be used to monitor early dynamic changes of HCC following TACE.

OBJECTIVETo discuss the results and significance of the detection of the CFTR gene mutation in azoospermia patients with congenital unilateral absence of the vas deferens (CUAVD).

METHODSWe collected peripheral blood samples from 6 azoospermia patients with CUAVD for detection of the CFTR gene mutations and single nucleotide polymorphisms. We analyzed the genome sequences of the CFTR gene in comparison with the website of the UCSC Genome Browser on Human Dec. 2013 Assembly.

RESULTSMissense mutation of c. 592G > C in exon 6 was found in 1 of the 6 azoospermia patients with CUAVD and splicing mutation of c. 1210-12T[5] was observed in the noncoding region before exon 10 in 2 of the patients, both with the V470 haplotype in exon 11.

CONCLUSIONMutations of the CFTR gene can be detected in azoospermia patients with CUAVD and the detection of the CFTR gene mutation is necessary for these patients.

Azoospermia ; genetics ; Cystic Fibrosis Transmembrane Conductance Regulator ; genetics ; Exons ; Humans ; Male ; Male Urogenital Diseases ; genetics ; Mutation, Missense ; genetics ; Vas Deferens ; abnormalities

BACKGROUND: Morroniside has been shown to play roles of anti-inflammation, antioxidant, anti-apoptosis, promoting vascular and neural regeneration, anti-platelet aggregation and neuroprotection in the rat model of ischemic brain injury, but whether it can inhibit the inflammatory reaction of cerebral hemorrhage is unclear. OBJECTIVE: To observe the changes of inflammatory factors (interleukin-1, tumor necrosis factor-α) and inflammatory-related proteins (nuclear factor-κB and SUMO2/3) as well as neurologic function in a rat model of cerebral hemorrhage treated with morroniside at different doses. METHODS: Healthy male Sprague-Dawley rats were randomly divided into sham operation, cerebral hemorrhage and low-, medium- and high-dose morroniside groups. The model of cerebral hemorrhage was established in the latter four groups by injecting autologous blood from the tail artery, followed by intragastric injection of 30, 90, 270 mg/kg morroniside in the three morronicide groups, respectively, three times daily for consecutive 7 days; the rats in the sham operation and model groups were given same volume of normal saline. Then, the neurological function was evaluated by Neurological Severity Scores; the brain tissue around the hematoma were removed to observe the morphological changes of neurocytes around the hematoma by hematoxylin-eosin staining; the expression levels of interleukin-1 and tumor necrosis factor α in the brain tissue were detected by ELISA; the expression levels of nuclear factor-κB and SUMO2/3 were detected by immunohistochemistry and western blot assay. RESULTS AND CONCLUSION: Compared with the icerebral hemorrhage group, the low-, medium- and high-dose morroniside groups showed a significnat neurological improvement, especially the high-dose group (P < 0.05). Compared with the sham operation group, the cerebral hemorrhage and morroniside groups exhibited a significant increase in the nerve function damage and expression levels of interleukin-1, tumor necrosis factor α, nuclear factor-κB and SUMO2/3 (P < 0.05). Compared with the cerebral hemorrhage group, in the low-, medium- and high-dose morroniside groups, the expression levels of interleukin-1 and tumor necrosis factor α were significantly reduced, and expression levels of nuclear factor-κB and SUMO2/3 were significantly increased (P < 0.05). In summary, high-dose morroniside can improve the neurological function in rats with cerebral hemorrhage by down-regulating the levels of inflammatory cytokines.

Objective To lay the foundation for further exploration on parasitifer's defence reaction to pneumolysin through constructing ply gene-deletion strain of Streptococcus pneumoniae and researching on its virulence change. Methods A linker fragment with erm gene in middle and homologous upstream and downstream fragment of ply gene at both sides was prepared by long flanking homology-polymerase chain reaction(LFH-PCR). The linker fragment was transformed into Streptococcus pneumoniae. ply-deficient strain was then screened out from blood plate which contains erythromycin and identified by PCR. ply-deficient strain growth in vitro was observed and virulence change was observed through infecting mouse model. Results PCR results showed that ply gene was replaced completely by erm gene. The ply deficient strain was successfully constructed. The growth of single strain culture medium showed that ply genetic defect made no influence on bacterial's external growth. While in the mice nasal cavity infecting experiment, deficient strain enter into blood after 6 h from infecting which obviously slower than that did wild-type(2 h). And the number of bacteria at each point was much smaller than that of wild-type(P ＜0. 01 ). The mice peritonaeum infecting experiment showed that median lethal time of wild-type was 3 d, while that of deficient strain was 18 d(P＜0. 01). Conclusion It is a good way to completely substitute ply gene using LFH-PCR. ply deletion made no influence on baterial's growth in vitro, but it resulting in reduction of bacterial virulence in vivo.

Hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase(HCT) is an key enzyme involved in the biosynthesis of chlorogenic acid in Lonicera japonica. In this study, eight putative HCT genes were cloned with RACE (rapid amplification of cDNA ends) technology based on the analysis of transcriptome in L. japonica. Among them, one was suggested as HCT gene (LjHCT) in L. japonica through analysis of sequence similarity, physical and chemical properties, and domain conservation of the proptein. LjHCT gene containing 1 275 bp encodes a protein with the molecular weight of 47 kDa. These results will provide foundation for exploring the function of LjHCT in Lonicera japonica.