1.Isolation, culture and identification of endothelial cells from rat glomeruli.
Yu ZENG ; Hong DENG ; Xiao-jun ZHOU ; Yun WANG
Chinese Journal of Pathology 2005;34(4):233-234
Animals
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Antigens, CD34
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metabolism
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Cell Culture Techniques
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methods
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Cells, Cultured
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Endothelial Cells
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cytology
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metabolism
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Female
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Kidney Glomerulus
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cytology
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metabolism
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Male
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Rats
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Rats, Sprague-Dawley
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Vimentin
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metabolism
3.Enhancers on the transmembrane transport of chlorogenic acid.
Jing REN ; Shengqi DENG ; Xuehua JIANG ; Lingling WANG ; Yu XIAO
Acta Pharmaceutica Sinica 2014;49(2):252-5
To investigate the influence of the difference enhancers on the transport mechanism of chlorogenic acid (CGA) across Caco-2 cells model, a RP-HPLC method was adopted to detect the concentrations of CGA. At the concentrations of 20 to 80 microg x mL(-1), the difference of absorption rate constants (K(a)) was not statistically significant. At the concentrations of 40 and 20 microg x mL(-1), the ratios of apparent permeability coefficients (P(app)) of the apical to basolateral and the basolateral to apical were 1.14 and 1.18, respectively. With the effect of enhancers K(a) and P(app) increased, the absorption half-life (T1/2) decreased. CGA passed through the Caco-2 cell membrane mainly by passive transport. It showed that monocarboxylic acid transporter (MCT) could be involved in the across membrane transport process of CGA. Borneol had no effect on the cell membrane transport processes. The order of increasing absorption of CGA caused by the enhancers was sodium lauryl sulphate > sodium taurocholate > carbomer.
4.Correlation of ST6 Gal-Ⅰ expression and atherosclerotic plaque reduction induced by rosuvastatin in ApoE-/- mice
Yan LIU ; Jun ZHANG ; Qianghong PU ; Xiao DENG ; Chao YU
Chinese Pharmacological Bulletin 2016;32(4):525-529,530
Aim To investigate whether rosuvastatin induced reduction of atherosclerotic plaque was related to the expression of Sialyltransferase ( ST6 Gal-Ⅰ) in ApoE-/ - mice. Methods Six-weeks old ApoE-/ -mice fed with high fat were divided randomly into three groups: baseline group ( n=12 ) , control group ( n=12 ) and rosuvastatin group ( n =12 ) . Sixteen weeks later, control group was sacrificed. Serum and aortic intima were saved. Control group and rosuvastatin group were fed for seven weeks continually. Concentra-tions of serum lipids(TC, TG, LDL and HDL) were analyzed. Sections from the aortic root were examined by Hematoxylin-Eosin( HE) staining. The size of ath-erosclerotic lesion in each section was evaluated. Ex-pression of ST6 Gal-Ⅰ in aortic intima was detected by immunohistochemistry. Results Plasma TG and LDL-C, plaque areas and intimal thickness of control group were significant higher than those of baseline group ( P<0. 05 ) . Those results indicated that the AS model was successfully constructed. After seven weeks, the plaque areas and concentrations of serum lipids of rosu-vastatin group were obviously smaller than those of con-trol group(P<0. 05). The expression of ST6Gal-Ⅰin aortic root was decreased in control group compared to the baseline, and which was increased in control group compared to the rosuvastatin group. Conclusion Ro-suvastatin could inhibit the progression of atherosclero-sis, which might be related to the expression of ST6Gal-Ⅰ in aortic root.
5.Study on morphological and immunophenotypic features of renal cell carcinoma with sarcomatoid differentiation.
Li XIAO ; Xiao-yu LU ; Ru-yong TANG ; Min DENG ; Xiong-zeng ZHU
Chinese Journal of Pathology 2007;36(4):259-260
Actins
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metabolism
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Aged
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Aged, 80 and over
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Carcinoma, Renal Cell
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metabolism
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pathology
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Cell Differentiation
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Female
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Humans
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Immunohistochemistry
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Keratin-7
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metabolism
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Keratin-8
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metabolism
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Kidney Neoplasms
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metabolism
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pathology
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Male
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Middle Aged
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Sarcoma
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metabolism
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pathology
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Vimentin
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metabolism
6.Diagnostic Value of Gap-Ligase Chain Reaction Enzyme Linked Immunosorbent Assay for Chlamydia Trachomatis Pneumonia in Neonatal and Small Infants
chun, DENG ; xiao-wen, LI ; jia-lin, YU ; shi-xiao, WU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the diagnostic value of Gap-ligase chain reaction(LCR)-enzyme linked immunosorbent assay(ELISA)for Chlamydia trachomatis(CT) pneumonia in infants(28 days-3 months old baby was 16.9%(27/160 cases),and the incidence in 3-6 months old baby was 8.1%(12/149 cases).The clinical symptoms included that 25 cases(51.0%) had fever,48 cases(98.0%) with paroxysmal cough,45 cases(91.8%) with rhinocleisis,45 cases(91.8%) with spitting foam,49 cases(100%) with tachypnea,28 cases(57.1%) with lips cyanosis,26 cases(53.1%) with medium and moist rales,24 cases(49.0%) with dry rales,18 cases(36.7%) with phlegm whimper,29 cases(59.2%) with rough breath sounds in both lungs and there were 13 cases(26.5%) with conjunctivitis.Chest film and paper capacitor showed that bilateral extensive interstitial and(or) alveolar infiltration,over-inflation 23 cases(46.9%) had no lobal consolidation or pleural effusion.The WBC counts were normal or slightly high.All children were treated with cephalothin or penicillin before confirmation with CT infection.Eleven cases were treated with azithromycin after diagnosis with CT pneumonia and were cured 9 days after treatment and the others were not treated with azithromycin so their course prolonged from 15 to 44 days,among them there were 11 out-patients who were treated many times because of repeated cough.Conclusions CT is important pathogenic organism to cause pneumonia in neonatal and small infants.It is important to pay attention to the possibility of pneumonia caused by CT and make the diagnosis in early period as soon as possible and treat them with sensitive drug to shorten the course.
7.532 laser photocoagulation combined with posterior sub - Tenon injection of triamcinolone acetonide in treatment of diffused diabetic macular edema
Xiao-Yan, HUANG ; Qin-Ying, YE ; Yu-Yin, HUANG ; Xiao-Ning, DENG
International Eye Science 2014;(6):1020-1022
AIM:To study the effect of posterior sub - Tenon's capsule injection of triamcinolone acetonide ( TA ) in treatment of patients with diffuse diabetic macular edema ( DME) before panretinal photocoagulation ( PRP) .
METHODS:Retrospective analysis of the clinical data of 96 cases (96 eyes) with DME treated in our hospital from October 2008 to May 2012, and the patients were divided into the study group and control group, each group with 48 cases ( 48 eyes ) , the control group were only treated with PRP, and for the study group, TA was injected one week before PRP. At 6mo after treatment, best-corrected visual acuity ( BCVA) and retinal thickness changes of two groups were compared, the changes of intraocular pressure in two groups was analyzed.
RESULTS:After treatment, two groups were followed up for 6mo, compared with before treatment, the expression of BCVA in control group was reduced, and rise in the study group, with significant difference between the two groups (P<0. 05), and during the follow-up period, IOP change was in the normal range for the two groups, with no the difference (P>0. 05), the study group had foveal thickness reduction of 9. 6μm, the control group was increased by 31. 9μm, with significant difference (P<0. 05), parafoveal thickness in the study group decreased 5μm, significantly increased 22. 1μm in the study group, centre concave surrounding thickness increased 0. 4μm in study group and 19. 4μm for the control group, with no significant difference (P>0. 05).CONCLUSION:TA injection in patients with diffuse DME before PRP is safe and effective, and it is superior to simple PRP therapy, and it can be applied in primary hospital.
8.Enhancers on the transmembrane transport of chlorogenic acid.
Jing REN ; Sheng-Qi DENG ; Xue-Hua JIANG ; Ling-Ling WANG ; Yu XIAO
Acta Pharmaceutica Sinica 2014;49(2):252-255
To investigate the influence of the difference enhancers on the transport mechanism of chlorogenic acid (CGA) across Caco-2 cells model, a RP-HPLC method was adopted to detect the concentrations of CGA. At the concentrations of 20 to 80 microg x mL(-1), the difference of absorption rate constants (K(a)) was not statistically significant. At the concentrations of 40 and 20 microg x mL(-1), the ratios of apparent permeability coefficients (P(app)) of the apical to basolateral and the basolateral to apical were 1.14 and 1.18, respectively. With the effect of enhancers K(a) and P(app) increased, the absorption half-life (T1/2) decreased. CGA passed through the Caco-2 cell membrane mainly by passive transport. It showed that monocarboxylic acid transporter (MCT) could be involved in the across membrane transport process of CGA. Borneol had no effect on the cell membrane transport processes. The order of increasing absorption of CGA caused by the enhancers was sodium lauryl sulphate > sodium taurocholate > carbomer.
Absorption
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Acrylic Resins
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pharmacology
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Caco-2 Cells
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Cell Membrane Permeability
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drug effects
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Chlorogenic Acid
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pharmacokinetics
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Humans
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Sodium Dodecyl Sulfate
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pharmacology
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Taurocholic Acid
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pharmacology
9.Expression of Ref-1 and FAP-1 mRNA in hypoxic-ischemic injury in neonatal rat.
Chun DENG ; Chun-bao GUO ; Jia-lin YU ; Shi-xiao WU ; Yi TAN
Chinese Journal of Pediatrics 2003;41(4):297-299
Animals
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Animals, Newborn
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Brain
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blood supply
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metabolism
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pathology
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Carrier Proteins
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genetics
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DNA-(Apurinic or Apyrimidinic Site) Lyase
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genetics
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Gene Expression
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Hypoxia-Ischemia, Brain
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genetics
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pathology
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In Situ Hybridization
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Protein Tyrosine Phosphatase, Non-Receptor Type 13
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Protein Tyrosine Phosphatases
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genetics
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Wistar
10.Association of ABO genotype with acute rejection in renal transplantation
Lixin YU ; Youcheng LIN ; Wenfeng DENG ; Yibin WANG ; Junsheng YE ; Yun MIAO ; Lulu XIAO
Chinese Journal of Urology 2010;31(12):814-817
Objective To investigate a possible association of donor-recipient compatibility for ABO blood group alleles with acute rejection (AR) in renal transplantation. Methods A study comprising 87 pairs of donor and recipient was performed. The ABO genotype A1, A2, O1, O2, and B alleles of renal transplanted recipients and their respective donors were assessed by PCR amplification with sequence-specific primers (PCR-SSP). Accordingly, recipients were divided into donor-recipient ABO genotype matched and mismatched groups. Results The PCR-SSP based types of all cases showed total concordance with their serologically assigned ABO groups. Fifty pairs (57. 5%) were matched for ABO genotype among the 87 pairs of donor and recipient while 37 (42. 5%) were mismatched, including 1 allele mismatch in 31 pairs (83.8%), 2 alleles mismatches in 6 pairs (16. 2%).The incidence of AR was 12.0% (6 cases) and 29. 7% (11 cases) for ABO genotype matched and mismatched transplant patients, respectively ( P < 0.05). After high dose methylprednisolone (MP)treatment, all cases exepienced reversion of AR except a A2O1 recipient receiving kidney from a A1O1enced 4 AR episodes within 3-10 months, and the period of AR was gradually shortened. After high dose MP was administered empirically, even though short-term improvement of renal function was observed, the serum creatinine continued to increase progressively with decreased efficacy of high dose MP. One year after operation the serum creatinine rose to 441 μmol/L. Conclusions Simultaneous definition of the ABO genotype and HLA is highly feasible. The A2 patient is suitable for receiving kidneys from blood group O donors. DNA mismatch for ABO genotype of renal transplant recipients and their respective donors is an independent risk factor for AR. Genotyping of ABO blood group is conducive to prevent AR.