Objective To explore the changes of blood 8-iso-Prostaglandin F_(2a)(8-iso-PGF_(2a))content in different time after building-model and study its significance,and study the intervention effect of N-acetylcysteine(NAC) on it.Methods Seven-day-old Sprague-Dawley (SD) rats were randomly divided into 3 groups:intervention,operation and false operation groups.rats in intervention group were administered with NAC 0.1 mg/(g?d),Rats in operation group were given an equal volume of normal saline,and rats in false group operation had no administration.The blood was obtained to determine serum of 8-iso-PGF_(2a) content in 30 minutes,on the first,third,seventh and twenty first day after the building-model,respectively.EIA assay method was used to determine serum 8-(iso-PGF)_(2a) content.Results Serum 8-iso-PGF_(2a) concentration in rats from operation group,intervention and false operation groups were(168.7?(24.2)),(120.9?23.4) and (50.0?8.8) ng/L within 30 minutes after the building-model, respectively.There were a remarkable difference between serum 8-iso-PGF_(2a) concentration in three groups(P0.05).Conclusions The concentration of serum 8-iso-PGF_(2a) of rats rises remarkably early after building-model, and the peak concentration appears at 30 minutes after the building-model. NAC can effectively decrease serum 8-iso-PGF_(2a) content, which suggest that there is large value of clinical administration.

Adult ; Bronchoalveolar Lavage ; methods ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; therapy ; Treatment Outcome

Objective To investigate the eftect of VEGF gene transtection on endothelial progenitor cell derived from murine bone marrow.Methods Wistar rat's bone marrow was obtained, mononuclear cell isolated,and endothelial progenitor cells(EPS)were cultured in EGM-2MV.EPCs were identified by immunocytochemistry and electron microscope.EPCs were transfected by liposome mediated pcDNA3.0-hVEGF165.VEGF protein level was determined in the cultural medium supernatant after VEGF transfection by ELISA.Cultural medium supernatant was used to co-culture with ECV304,VEGF protein activity was evaluated by MTT.EPCs expression of vWF,VEGF,FLK-1 was detected by immunocytochemistry.Results EPCs were effectively enriched by EGM-2MV,and the EPCs obtained express the typical cell surface markers such as CD34,CD133,FLK-1.The concentration of VEGF protein in supernatant reaches 1280 pg/ml in the 7th day after pcDNA3.0-hVEGF transfection.No influence of EPCs proliferation could be found after transfeetion.The cell surface marker expression of VEGF,FLK-1, vWF became higher with time,and the ratios of positive cell were 88.52%,82.65% and 95.97% respectively.Conclusions pcDNA3.0-hVEGF165 transfeet EPCS mediated by liposome could excrete a high concentration of functional VEGF protein.It is helpful for EPC to maintain the characters of endothelial cell after VEGF gene transfection and differentiate to mature endothelial cell.

Chromosomes, Human, Pair 22 ; Chromosomes, Human, Pair 9 ; Humans ; Leukemia, Promyelocytic, Acute ; genetics ; Male ; Middle Aged ; Translocation, Genetic

Acupuncture Points ; Aged ; Aged, 80 and over ; Bloodletting ; Female ; Humans ; Middle Aged ; Pruritus ; therapy

Actins ; metabolism ; Adult ; Breast ; pathology ; Breast Neoplasms ; metabolism ; pathology ; surgery ; Female ; Humans ; Mammography ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Lung metastasis is one of the most common metastases.Metastasectomy is only indicated for selected patients,and most patients are unsuited to surgery.The main treatment is systemic chemotherapy,however,the long-term survival is limited.With the development of precision radiotherapies such as threedimensional conformal radiation therapy (3D-CRT) and stereotactic body radiotherapy (SBRT),it is proved that radiotherapy is favorable for the patients with lung metastasis,especially for limited lung metastasis.SBRT can be obtained better survival,but still need large prospective studies.

Cardiovascular disease is a major cause of morbidity and mortality in the contemporary society. Cardiac gene therapy has already been investigated in clinical studies profoundly. Adenovirus vector could transfer the gene efficiently, but low organ specificity and immuno- genie properties limited its application, With adenovims vector improved in the last few years, the third adenovims vector is probably the most potential vector systems for cardiovascular disease. This review will give a broad overview of the molecular basis of the adenovims vectors, their advantages, the development of increasingly efficient gene transfer approach, and experimental and clinical studies targeting the heart, application barriers and modified strategies.

目的观察中药与手法结合治疗腰椎间盘突出症的疗效。方法对66例腰椎间盘突出症患者采用中药与手法结合治疗,2个月后观察其疗效。结果中药与手法治疗的总有效率为86.37%。结论中药与手法结合治疗腰椎间盘突出症可获得满意疗效,且疗程1个月内疗效最为明显。

OBJECTIVE To study the analytic method for assaying of glutaral concentration by enzyme-labeled device at one time.METHODS Controlled solution of glutaral and sample solution were taken out and kept until the room temperature.Optical density value was detected at 450 nm.Content of sample was calculated by comparison with the known concentration of glutaral.RESULTS In the range of 0.75-2.50%,concentration of glutaral had linear relationship with the optical density value.Reproductive test:relative average error was within 2%,RSD≤2.51%;interference test:oxidation-resistant-sodium nitrite could make the result negative error.0.1% Sodium nitrite could make the test result decrease by 1.5% relatively.CONCLUSIONS The method is simple,fast and convenient,with accuracy which has met the requirement of hospital infection monitoring and acceptable interfering range made by additives,which is highly practical.