1.Effects of Borneol on Membrane Fluidity and Membrane Potential of HaCaT Cell
Dali FU ; Xiaolan YONG ; Defang LIU ; Shuang XIAO
Chinese Journal of Information on Traditional Chinese Medicine 2015;(4):62-66
Objective To investigate the action mode of borneol on activity of epidermal skin;To investigate action mode of borneol as penetration enhancer. Methods The well-established and standard penetration enhancer Azone was employed as a positive control in this study. The cytotoxicities of borneol and Azone on HaCaT cells were detected by CCK-8 assay, and their half 50% inhibitory concentrations (IC50) were calculated. The fluorescence recovery after photo bleaching was employed to investigate the effect of borneol and Azone on membrane fluidity, and the flow cytometer was used to monitor the changes of membrane potential of HaCaT cell after treated with these penetration enhancers. Results The IC50 values of borneol and Azone were 2.826 , 0.172 mmol/L, respectively. Borneol could significantly improve the membrane fluidity in a concentration-dependent manner, and effectively decrease the membrane potential of HaCaT cell, which exhibited the performances similar to those of Azone. Conclusion The penetration enhancement mechanism of borneol was associated with the concentrations of Ca2+ in keratinocytes, which changes the membrane fluidity and membrane potential of HaCaT cell.
2.Expression of PED/PEA-15 and XIAP in prostate cancer cells and their effects on prostate cancer cell (PC-3) apoptosis
Xiao-Yong HU ; Xiao-Chun CHEN ; Zhao-Hui ZHU ; Fu-Qing ZENG ; Gong-Cheng LU ;
Chinese Journal of Geriatrics 2001;0(03):-
Objective To study the effect of antiapoptosis factors PED/PEA-15 and XIAP on prostate cancer cells(PC-3)apoptosis.Methods The expressions of XIAP and PED/PEA-15 in prostate cancer cells(PC-3)were respectively assayed using the RT-PCR technique.XIAP and PED/ PEA-15 specific siRNA vectors were designed and constructed and then were transiently cotransfected into PC-3 cells under induction of liposome.The effects of siRNA vectors on PED/PEA-15 and XIAP transcription were assayed by RT-PCR technique,and the effect of XIAP and PED/PEA-15 on cancer cell apoptosis were determined by flow cytometry and microscope observation.Results PED/PEA- 15 and XIAP were both highly expressed in PC-3 cells.Enzyme digestion analysis and DNA sequencing confirmed that the PED/PEA-15 and XIAP-specific siRNA expression vectors were constructed successfully.The designed siRNA sequences of PED/PEA-15 and XIAP could specifically inhibit their transcription.The PC-3 cells which were cotransfected with PED/PEA-15 and XIAP- specific siRNA vectors were more sensitive to doxorubicin.The apoptosis rate of cotransfected cells was significantly increased.Conclusions PED/PEA-15 and XIAP might be involved in the development of prostate cancer.
3.Development and application of network system in radiotherapy
Zhi-Yong XU ; Xiao-Long FU ; Liang SONG ; Xiao-Hua CHEN ; Kai SUN ;
Chinese Journal of Radiation Oncology 2005;0(05):-
Objective To develop a network system for radiotherapy.Methods Delphi 6.0 lan- guage was used to program the system based on PACS through the model of client-server machine and local network.Data of different facilities were transferred among each other through Dicom 3.0 and Dicom RT pro- tocol.Results The main function of this system was a management software for radiotherapy,a PACS sys- tem,a TPS system and a therapeutic machine system.Conclusion The network system operates steadily with data safe and reliable,and is an important part of the information construction in the department of radio- therapy
4.Anatomical and clinical study on partial pectoralis minor transplantation for reconstruction of the opposition of the thumb
Yong-Qing ZHUANG ; Hong-Tao XIONG ; Qiang FU ; Hao-Li JIANG ; Xiao-Kuan FU ; Dong DU ; Jing TONG ;
Chinese Journal of Microsurgery 2006;0(05):-
Objective To investigate a new surgical method by using pectoralis minor muscle for its partial transplantation to reconstruct the function of the opposite thumb anastomosed vessels and nerves. Methods The proposed method was evaluated by taking 20 cases of adult cadaveric thoraxes and hands,then compared with the morphology and dimension of both pectoralis minor muscle and palmer muscle to study the feasibility of the new method.Based on the observed data.We selected five suitable cases,in which the opposi- tion function was lost,and then applied the new operation on them with partial transplantation of pectoralis mi- nor muscle anastomosed vessels and nerves according to the results of anatomic study.The follow-up study was conducted to observe the functional recovery of opposition of the thumb.Results The main results can be summarized as follows.First,the anatomical position of pectoralis minor muscle was stable,and every peetoralis minor muscle was provided with self-sustaining artery,vein and nerve.The oppostition process of cadaveric hand succeeded after similar transplantation to clinic operation.Second,follow-up studies conducted 6 - 12 months after the operation showed that all five patients recovered fully.The muscle strength in all five cases re- covered to level four or higher.The shape of palm eminence was satisfactory.Conclusion The surgical method of pectoralis minor muscle transplantation for reconstructing the opposition function of the thumb was based on the clinical and anatomical application.The function of opposition of the thumb reached the satisfacto- ry requirement after the operation.So,the new surgical method can achieve better results than other existing operation methods.
5.Study on the effect of combined therapy of Chinese and Western medicines and gene therapy (GT) on cartilage end-plate calcification in rabbits with cervical intervertebral disc regression.
Fu-cheng LIU ; Xiao-yong ZHAO ; Hai-tao WANG
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(10):907-911
OBJECTIVETo study the relationship between cartilage end-plate calcification (CEC) and cervical intervertebral discs regression (CIDR) in rabbits, and to study the inhibitory effect of combined therapy of Kanggu Zengsheng Capsule (KZC) ansforming growth factor-apsule (TGF-PLC) and igene therapy (GT) on CEC by measuring the thickness of CEC layer.
METHODSThirty-five New Zeland rabbits of 4 months old were selected to establish cervical dynamic imbalance rabbit model for inducing CIDR (No disposal was given to rabbits in the normal control group). Seven months after operation, combined therapy of KZC and PLC were given, in doses calculated by body weight, to the modeled rabbits in the drug treated group with CEC of either superficial layer or full layer, twice a dantly by gavage for 30 successive days. While to those in the gene therapy group, the recombinant plasmid DNA with transforming growth factor-beta1 (TGF-beta1) was injected once their intervertebral discs (ID) of C(2-3) C(3-4) and C(4-5), 20 microl for each injection. One month later, all rabbits were sacrificed with periotic venous gas embolic method and their ID of C(4-5) (including partial body of the upper and lower vertebrae) was resected. The degree of CIDR was evaluated morphologically, and the thickness of CEC in rabbits was measured and compared between groups.
RESULTSThickness of CEC in the model group, either of superficial layer or of full layer, was significantly more than that in the normal control group with significant difference. Both combined KZC and PLC therapy and gene therapy showed significant inhibitory effects on CEC in treating CIDR (P < 0.05).
CONCLUSIONCEC is the initial factor of CIDR with highly positive correlation. Both combined therapy of KZC and PLC and gene therapy can significantly inhibit CEC.
Animals ; Calcinosis ; complications ; pathology ; Cartilage ; pathology ; Cervical Vertebrae ; pathology ; Combined Modality Therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Genetic Therapy ; Intervertebral Disc ; pathology ; Male ; Phytotherapy ; Rabbits ; Random Allocation ; Spinal Diseases ; complications ; pathology ; therapy
6.Current Situation and Thinking of Statistical Management of TCM
Yong XIAO ; Shaowu SHEN ; Wenjiao FU ; Ting ZHOU ; Shuanggui TIAN ; Na ZHAO
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(2):5-8
Objective To understand the current situation of statistical management of TCM;To analyze existing problems; To provide references for policy formulation and measure implementation for statistical management of TCM. Methods Literature research, field research, comparative analysis, questionnaire surveys, expert consultation, and Fisher's exact test were used in the study. Results It was found that China’s statistical management of TCM had the problems of imperfect system, unestablished investigation system, incomplete statistical index system, and nonstandard statistical management. No statistical difference was found in the three regions of east, middle and the west. Conclusion Management and investigation system should be established in statistical management of TCM; pilot projects should be conducted before they are spread;information sharing and data mining should be strengthened.
7.Treatment of diabetic foot with microsurgical operation
Dong DU ; Yong-Qing ZHUANG ; Xiao-Kuan FU ; Jing TONG ; Hao-Li JIANG ; Yi-Jian YANG
Chinese Journal of Microsurgery 2006;0(06):-
Objective To report clinical study of diabetic foot with microsurgical treatment.Methods 32 cases basing on physical treatment underwent operation which included reconstruction of vessel under DSA and flap transfer and relaxation of nerves.Results 8 eases were examined with DSA after operation,it showed that the bypass grafts were unobstructed and the distal blood were improved;All flap were lively. Conclusion The ulcer of the patients with diabetic foot was closed early and the blood supply of the limb have been reconstructed by microsurgical treatment,it can not only avoid amputation or lower the limb amputation level,but also improve the life quality of patients and obtain social benefit.
8.Atypical meningioma of left lateral sulcus with chondroid metaplasia: report of a case.
Yan REN ; Ai-Jun LIU ; Xiao ZOU ; Fu-Yong WANG ; Jin-Wu WANG
Chinese Journal of Pathology 2008;37(1):65-66
Adult
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Female
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Humans
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Meningeal Neoplasms
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complications
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pathology
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Meningioma
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complications
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pathology
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Metaplasia
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complications
9.Study on effect of aqueous extracts from aconite on "dose-time-toxicity" relationships in mice hearts.
Qun FENG ; Xiao-yu LI ; Yong-fu LUAN ; Sai-nan SUN ; Rong SUN
China Journal of Chinese Materia Medica 2015;40(5):927-932
OBJECTIVETo study the effect of single administration of aqueous extracts from aconite on "dose-toxicity" relationship and "time-toxicity" relationship of mice hearts, through changes in electrocardiogram (ECG) and serum biochemical indexes.
METHODMice were grouped according to different drug doses and time points, and orally administered with water extracts from aconite for once to observe the changes of mice ECG before and after the administration, calculate visceral indexes heart, liver and kidney, and detect levels of CK, LDH, BNP and CTn-I in serum.
RESULTAccording to the "time-toxicity" relationship study, at 5 min after oral administration with aqueous extracts from aconite in mice, the heart rate of mice began rising, reached peak at 60 min and then slowly reduced; QRS, R amplitude, T duration and amplitude and QT interval declined at 5 min, reduced to the bottom at 60 min and then gradually elevated. The levels of CK, LDH, BNP and CTn-I in serum elevated at 5 min and reached the peak at 60 min, with no significant change in ratios of organs to body at different time points. On the basis of the "dose-toxicity" relationship, with the increase in single dose of aqueous extracts from aconite, the heart rate of mice. QRS, T duration and amplitude and QT interval declined gradually, and levels of CK, LDH, BNP and CTn-I in serum slowly elevated, with a certain dose dependence and no significant change in ratios of organs to body in mice.
CONCLUSIONSingle oral administration of different doses of aqueous extracts from aconite could cause different degrees of heart injury at different time points, with a certain dose dependence. Its peak time of toxicity is at 60 min after the administration of aqueous extracts from aconite.
Aconitum ; adverse effects ; chemistry ; Animals ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; toxicity ; Female ; Heart ; drug effects ; physiology ; Heart Rate ; drug effects ; Kidney ; drug effects ; Liver ; drug effects ; Male ; Mice
10.Histological observation on new bone induced by platelet-rich fibrin
Dongmei FU ; Qiong XIAO ; Qinqiu YANG ; Lu DONG ; Hongliang CHEN ; Yong SUN
Chinese Journal of Tissue Engineering Research 2016;20(7):933-939
BACKGROUND:In previous experiments, we have confirmed that platelet rich fibrin has the ability of osteoinduction, and have conducted a preliminary study on its microstructure and biomechanics. However, little is reported on its histology research.
OBJECTIVE:To compare the histological changes after implanting platelet-rich fibrin, Bio-Oss and autologous bone and to analyze the pros and cons of platelet-rich fibrin implantation for repair of bone defects.
METHODS: As previously reported, animal models of critical bone defects were established respectively on the bilateral femoral condyles of 12 beagle dogs. Then, platelet-rich fibrin, Bio-Oss+colagen membrane (Bio-Oss group) and autologous bone (autologous bone group)+colagen membrane were respectively implanted. At 3, 6, 8 and 12 months, one experimental dog from each group was kiled, respectively, and histological observation was performed. Another beagle dog as blank control was enroled to establish the animal model of critical bone defects, with no implantation.
RESULTS AND CONCLUSION:At 3, 6, 8 and 12 months after implantation, there were significant differences in the new bone formation speed and amount between the platelet-rich fibrin group, Bio-Oss group and autologous bone group. These three kinds of bone grafts al had osteoinductive ability to different extents. In the platelet-rich fibrin group, the osteogenic effects were better at 3 and 6 months, and the new bone was similar to natural one; in the autologuos bone group, bone necrosis was noticeable at 3 and 6 months, but the osteogenic effects became better at 8 months, and the new bone was similar to natural one at 12 months; in the Bio-oss group, the osteogenic effects were similar to those in the platelet-rich fibrin group, but the residual of Bio-oss was visible at 12 months; in the blank control group, no bone formed at 3 months, indicating the animal model of critical bone defects was made successfuly. In brief, the platelet-rich fibrin has good osteoinductive ability, with shorter time and better quality.