1.INFLUENCE OF EXUDATES OF AMMONIUM-RESISTANT N_2 -FIXING BACTERIA ON THE GROWTH OF RICE SEEDLING
Ming-Qi QIU ; Qiu-Yan LU ; Hong-Hui ZHU ; Xiao-Ying QIU ; Yuan-Sheng QIU ;
Microbiology 1992;0(04):-
By using cup culture, influence of different strains (ammonium-resistant N2-fixing type, wild type, none N2-fixing type) of Klebsiella oxytoca on the growth of rice seedling was compared. It was discovered that ammonium-resistant N2 -fixing bacteria could excrete some plant growth promoting substance, which could be adsorbed by cation resin. It' s activity wouldn't be affected at 80℃. At optimal concentration, the weight of rice root and seedling were increased by
2.STUDY ON HIGH-DENSITY CULTURE OF AMMONIUM-RESISTANT N_2-FIXING BACTERIUM KLEBSIELLA OXYTOCA
Qiu-Yan LU ; Xiao-Bao XIE ; Hong-Hui ZHU ; Xiao-Ying QIU ; Ming-Qi QIU ; Yuan-Sheng QIU ;
Microbiology 1992;0(02):-
Based on the physiological properties of ammonium-resistant N2-fixingbacterium ( Klebsiella oxytoca NG13/pMC73A), the fermentation technology of it was studied. The basic medium of high-density culture was established, with glucose as carbon source coupled with appropriate nitrogen source and inorganic salts. At the middle and late phase of culture, glucose and ammonia were added to supply carbon source and nitrogen source, stabilizing the pHat 6.5 ~ 6.8. Optimal level of dissolved oxygen was kept by controlling aeration and stirring rate. Bacterium number of Klebsiella oxytoca NG13/pMC73A reached 600 ~ 700 x 10s cfu/mL at the end of culture. Compared with previous technology, bacterium number was increased by more than ten-fold with a comparable culture period.
6.Effect of 5-Azacytidine on In-Vitro Proliferation and Myogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
Kaijia CHEN ; Xiaobin LIU ; Shijun QIU ; Jianhong ZHOU ; Ying XIAO
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(05):-
0.05),but 5-Aza at the concentration of 15?mol/L inhibited the growth of MSCs(P
7.Impact of lead on cytotoxicity in NRK cells and interference of calcium antagonist.
Xiao-Ting LU ; Qiu-Ying LI ; Hui-Fen GUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):358-360
Calcium Channel Blockers
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pharmacology
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Cell Survival
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drug effects
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Drug Antagonism
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Humans
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Kidney
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cytology
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drug effects
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Lead
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toxicity
8.Primary Discussion on Ability Culture of Interns in Anaesthesiological Department
Qi-Ying LI ; Xiao-Qiu YANG ; Su MIN ;
Chinese Journal of Medical Education Research 2006;0(07):-
Objective:to culture excellent bachelor majoring in anesthesiology.Methods:to make teaching plan for practice in anesthesiological department with emphasis on following aspects:culture of emotional intelligence;development of the sense of responsibility for work;training of clinical thought to combine theory and practice and training of basic techniques;formation of good relationship and communication with the others and culture of scientific research ability.Results:All faculties execute the plan carefully,and all the interns graduate with clinical and research ability as expected.Conclusion:It is very important for ability improvement of interns to make and execute available plan,keep strict and careful attitude,train their clinical thought and basic techniques,and emphasis the sense of responsibility for work.
9.STUDY ON THE CHARACTERS OF AN HARPIN-PRODUCING BACTERIA STRAIN TOLERANT TO AMMONIUM AND NITROGEN-FIXING
Hong-Hui ZHU ; Yan-Qin LI ; Xiao-Ying QIU ; Ming-Qi QIU ; Li-Ping ZHAO ;
Microbiology 1992;0(04):-
The ability to induce hypersensity on leaves of tomato and the stability of double-plasmid of an harpin-producing, nitrogen-fixing engineered strain E4 were tested. Hypelsensitivity-inducing experiment indicated that the time and density of hypersensitivity-induction of E4 was similar to those of DH5, the positive control of pCPP430. Although E4 took the same time to induce hypersensitivity as 308R, another positive control of pCPP430, it induced weaker hypersen- sitivity on tobacco leaves. On tomato leaves, there was no difference in time and density of hypersensitivity between E4 and 308R (pCPP430). Results revealed that the two plasmids, pCPP430 and pMC73A, were unstable in host bacteria, with the losing rate of 100% at the 48th generation. The emergence probability of bacteria with either pCPP430 or pMC73A was almost the same.
10.Hospital Infection in EICU Ward: Investigation and Analysis
Xiao-Li SONG ; Ying HOU ; Hui LV ; Xi-Yun GUO ; Xiao-Qiu YANG ;
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To investigate the hospital infection rate and sites of patients in emergency intensive care(unit)(EICU),and provide basis for prevention and treatment of hospital infection.METHODS Patients who were hospitalized in EICU were investigated by retrospective study.RESULTS Among the hospital infection sites,(respiratory) tract was the most frequent one(65.63%),the next was urinary tract(28.13%),and the deep vein was the third(6.25%).The most common hospital infection bacteria were Staphylococcus aureus,Pseudomonas aeruginosa and Acinetobacter baumannii.CONCLUSIONS The analysis of the subjective and objective factors of hospital infection,and the acknowledge of relationship between nursing and hospital infection sites are(important) basis for hospital infection prevention and treatment.