1.Study on the mechanism of moxibustion for rheumatoid arthritis based on liquid chromatography-mass spectrometry
Xiangtian PANG ; Yufei LENG ; Yao YAO ; Danwen WANG ; Cheng LI ; Xiao XU ; Zhiling SUN
Journal of Acupuncture and Tuina Science 2022;20(4):247-256
Objective: To explore the mechanism of moxibustion for rheumatoid arthritis (RA) by observing the metabolite changes in urine using liquid chromatography-mass spectrometry (LC-MS)-based metabolomic analysis. Methods: Twenty-four rats were randomly divided into a control group, a model group, and a moxibustion group. Rats in the model and moxibustion groups were established as collagen-induced arthritis (CIA) models. The control and model groups did not receive any intervention; rats in the moxibustion group received moxibustion at Shenshu (BL23) and Zusanli (ST36). After three weeks of intervention, ankle joint, serum, and urine samples were collected for pathological examinations and metabolomic tests. Results: After moxibustion treatment, the CIA rats showed increased body mass, reduced swelling of the hind paws and arthritis score, decreased serum cytokine levels, and improved histopathological evaluation of the ankle joint. Twenty-four significantly altered metabolites were found, mainly involved in alanine metabolism, taurine and hypotaurine metabolism, tricarboxylic acid cycle, phenylalanine metabolism, tyrosine metabolism, and primary bile acid biosynthesis. These metabolites may serve as potential biomarkers for RA. Conclusion: Moxibustion can effectively resist inflammation in CIA rats. The potential biomarkers and the abnormal metabolic pathways of RA can be identified by LC-MS-based metabolomics. Metabolomics may be an effective way to explain the mechanism of moxibustion in treating RA.
3.Analysis on the founding and management of the National Natural Science Foundation of Chongqing Medical University from 2013 to 2018
Xiaoli DU ; Xiaoling LI ; Jun YUAN ; Yao LENG ; Xiao YANG
Chinese Journal of Medical Science Research Management 2019;32(6):435-440
Objective The purpose of this paper is to study the problems and challenges faced by the university in the management of science funds,in order to provide evidence for improving the management level of science funds at university,at the same time to provide possible reference for other universities.Methods Through retrospective analysis of the application and funding of the National Natural Science Foundation of China of Chongqing Medical University during the period of 2013 to 2018.Results In recent years,the funding level of science funds in our university has entered the bottleneck period,main influencing factors include policy,talents,management and so on.Conclusions It is suggested to strengthen the top-level design of scientific research management,strengthen the training of scientific research personnel,and ensure the capability of scientific research management.
4.Expressional changes of CLC-2 and CLC-3 chloride channel in the hippocampus of rats with lithium-pilocarpine-induced epilepsy
Ya-Bo FENG ; Hong YAO ; Zhao-Fu CHI ; Xiao MAN ; Yi-Feng DU ; Zai-Ying PANG ; Zhen-Pu LENG
Chinese Journal of Neuromedicine 2010;9(3):222-226
Objective To investigate the distribution and expression changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat models with lithium-pilocarpine-induced chronic epileptic,and discuss the significance of these changes in epileptic pathogenesis.Methods Eighty Wistar rats were randomly divided into status epilepticus(SE,n=60)and control(n=20)groups and rats in the SE group were assigned to 3 subgroups according to the different sacrificed times(24 h,14 d and 30 d).Rat models with chronic epileptic in the SE group were induced by lithium-pilocarpine.Immunohistochemistry was employed to observe the changes of voltage-gated chloride channel CLC-2 and CLC-3 in rat's hlppocampal formation different time points after seizure.The mRNA changes of chloride channel CLC-2 and CLC-3 at different time points after seizure were observed by RT-PCR.Results Compared with those in the control group,the quantity of CLC-2 immune positive neurons and the average optical density in the SE group decreased obviously 14 and 30 d after seizure;so was the mRNA expression of CLC-2(P<0.05).Compared with the control group,the SE group showed obviously increased quantity of CLC-3 immune positive neurons and optical density at each area of the hippocampus 24 h after seizure;so was the mRNA expression of CLC-3(P<0.05).Conclusion Seizures at chronic phase have relation with the decreased expressions of CLC-2.
5.Ganglioglioma: a clinicopathological study of 19 cases
Le LIANG ; Jing FU ; Da-Sheng LI ; Hui LENG ; Yong-Ling LIU ; Xiao-Xiang YAO ; Ri-Le GE ; Yun-Lin LI ; Kang-Ping MA
Chinese Journal of Clinical and Experimental Pathology 2018;34(3):273-278
Purpose To study the clinicopathologic features of ganglioglioma. Methods The clinicopathologic data of the cases pathologically diagnosed as ganglioglioma that underwent resection of epileptic focus were retrospectively analyzed. Results In the 19 cases studied, the mean onset age was 9.1 years, and the duration of disease was 9.3 years. MRI images showed abnormal signals. The majority of the site was temporal lobe (14/19, 73.7%). The tumors showed heterogeneity and often accompanied by focal cortical dysplasias (13/19, 68.4%). Immunohistochemical staining showed CD34 positive in 18 cases, Nestin positive in 16 cases, and BRAF-V600E positive in 6 case. The positive expression rate of CD34 and Nestin did not have significant differences. Conclusion The diagnosis of ganglioglioma relies on pathological observations combined with clinical features and neuroradiological examinations. Differential diagnosis should be done from other tumors or cortical dysplasia. Immunohistochemical staining of CD34 and Nestin can help diagnosis.
6. "Watch and wait" strategy after neoadjuvant therapy for rectal cancer: status survey of perceptions, attitudes and treatment selection in Chinese surgeons
Tingting SUN ; Lin WANG ; Yunfeng YAO ; Yifan PENG ; Jun ZHAO ; Tiancheng ZHAN ; Jiahua LENG ; Hongyi WANG ; Nan CHEN ; Pengju CHEN ; Yingjie LI ; Xiao ZHANG ; Xinzhi LIU ; Yue ZHANG ; Aiwen WU
Chinese Journal of Gastrointestinal Surgery 2019;22(6):550-559
Objective:
To understand the perceptions, attitudes and treatment selection of Chinese surgeons on the "watch and wait" strategy for rectal cancer patients after achieving a clinical complete response (cCR) following neoadjuvant chemoradiotherapy (nCRT).
Methods:
A cross
7.Cataract-causing mutation S228P promotes βB1-crystallin aggregation and degradation by separating two interacting loops in C-terminal domain.
Liang-Bo QI ; Li-Dan HU ; Huihui LIU ; Hai-Yun LI ; Xiao-Yao LENG ; Yong-Bin YAN
Protein & Cell 2016;7(7):501-515
β/γ-Crystallins are predominant structural proteins in the cytoplasm of lens fiber cells and share a similar fold composing of four Greek-key motifs divided into two domains. Numerous cataract-causing mutations have been identified in various β/γ-crystallins, but the mechanisms underlying cataract caused by most mutations remains uncharacterized. The S228P mutation in βB1-crystallin has been linked to autosomal dominant congenital nuclear cataract. Here we found that the S228P mutant was prone to aggregate and degrade in both of the human and E. coli cells. The intracellular S228P aggregates could be redissolved by lanosterol. The S228P mutation modified the refolding pathway of βB1-crystallin by affecting the formation of the dimeric intermediate but not the monomeric intermediate. Compared with native βB1-crystallin, the refolded S228P protein had less packed structures, unquenched Trp fluorophores and increased hydrophobic exposure. The refolded S228P protein was prone to aggregate at the physiological temperature and decreased the protective effect of βB1-crystallin on βA3-crystallin. Molecular dynamic simulation studies indicated that the mutation decreased the subunit binding energy and modified the distribution of surface electrostatic potentials. More importantly, the mutation separated two interacting loops in the C-terminal domain, which shielded the hydrophobic core from solvent in native βB1-crystallin. These two interacting loops are highly conserved in both of the N- and C-terminal domains of all β/γ-crystallins. We propose that these two interacting loops play an important role in the folding and structural stability of β/γ-crystallin domains by protecting the hydrophobic core from solvent access.
Amino Acid Substitution
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Cataract
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genetics
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metabolism
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HeLa Cells
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Humans
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Molecular Dynamics Simulation
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Mutation, Missense
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Protein Aggregation, Pathological
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genetics
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metabolism
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Protein Domains
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Protein Structure, Secondary
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Proteolysis
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beta-Crystallin B Chain
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chemistry
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genetics
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metabolism
8.Herbal Textual Research and Processing Historical Evolution of Rhododendri Mollis Flos
Xiao-hong GUO ; Jing-wen FENG ; Yao-deng WANG ; Wen-hui WU ; Hong-ping CHEN ; Jing LENG ; You-ping LIU
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(22):172-180
In this paper, through the collection and collation of ancient materia medica, medical books and medical formulary, combining with modern literature, the historical changes of the name, origin, position, harvesting time, medicinal parts, toxicity, functions and indications, processing methods of Rhododendri Mollis Flos (RMF) were systematically combed and verified, so as to provide reference for clinical application, processing standard and basic research of RMF. According to textual research, RMF is the dried flower of
9. Salvianolic acid A inhibits platelet and neutrophil activation
Wen-Jie YANG ; Lu-Lu MA ; Le-Yu FANG ; Chun-Xiao LI ; Lu-Sha ZHANG ; Li-Yuan ZHANG ; Qian-Yi WANG ; Wei SUN ; Yu-Ze LENG ; Yue-Jin XUE ; Meng-Yao LI ; Hong WANG ; Hong-Ying WANG ; Lu CHEN
Chinese Pharmacological Bulletin 2022;38(5):740-748
Aim To explore the effeets of Salvianolie aeirl A (SAA) on platelet recruitment, activation and neutrophils in heart of myocardial infarction ( Ml ) mice.Methods C57BL/6 mice were randomly divid¬ed into: Sham operation group.Ml model group, SAA (5, 10 mg • kg 1 ) group, tirofiban (Tirofiban, 0.87 mg • kg ' ) group, using tail vein injection for 3 d.Echocardiography and HE staining were used to detect mouse heart function and infarct area; 1HC, FCS, ELISA, Western blot and other methods were used to explore the inhibitory effect of SAA on platelet and neutrophil activation.Results Compared with Ml group, SAA could improve the cardiac function and cardiac physiology changes of Ml mice, reduce the ex¬pression of CD42c in myocardial tissue and CD62p in peripheral blood without affecting tail bleeding time, reduce ADP-induced platelet activation and increase p- VASP/VASP ratio, reduce the ratio of p-PI3K/PI3K and p-AKT/AKT, reduce the expression of CD45, Ly6G, CXCL1 and CXCL2 in myocardial tissue, re¬duce the expression of complement component C3aR in myocardial tissue, and reduce C3a-induced NE and MPO, MMP9, LF level.Conclusions SAA has an anti-platelet activation effect by inhibiting the PI3K/ AKT and VASP pathways and an anti-neutrophil acti¬vation effect by inhibiting the expression of C3aR and C3a.
10.Short-term outcome of transanal total mesorectal excision for male low rectal cancer patients with "difficult pelvis" : a single center report from Peking University Cancer Hospital.
Aiwen WU ; Guoli HE ; Lin WANG ; Qiushi DONG ; Xinzhi LIU ; Yingjie LI ; Jiahua LENG ; Xiao ZHANG ; Tingting SUN ; Yue ZHANG ; Yunfeng YAO
Chinese Journal of Gastrointestinal Surgery 2018;21(6):646-653
OBJECTIVETo explore the applicable value of transanal total mesorectal excision (taTME) in male low rectal cancer patients with narrow pelvis-"difficult pelvis", which remains difficult for both open and laparoscopic sphincter-saving operations.
METHODSClinical data of male low rectal cancer patients diagnosed by pathology undergoing taTME between June 2016 and January 2018 at Peking University Cancer Hospital were collected. A retrospective cohort study was performed. Patients were selected according to the following criteria: (1) low rectal cancer, the distance between inferior margin of tumor and anal verge ≤5 cm; (2) the distance between two sciatic tubercles <5 cm; (3) body mass index (BMI) >25 kg/m; (4) tumor horizontal diameter ≤4 cm. Operation time, intraoperative blood loss, postoperative hospital stay, postoperative complications and anal function were analyzed.
RESULTSA total of 20 patients were included in this study. All the patients received preoperative neoadjuvant chemoradiation and hybrid transabdominal and transanal surgery. The median BMI was 27.7(26.2-36.4) kg/m; the median distance between two sciatic tubercles was 92.5 (78-100) mm; the median distance between the inferior margin of tumor to the anal verge was 4 (2-5) cm; the median operation time was 302 (215-402) min; the median intraoperative blood loss was 100 (50-200) ml; the median postoperative hospital stay was 9 (5-15) d. Postoperative complications occurred in 5 patients (25%), including 3 pelvic infection, 1 intestinal obstruction, 1 anastomotic leakage receiving sigmoid colostomy. There was no perioperative death. Sphincter-preservation rate was 100%. Nineteen patients received anal manometry 1 month after operation with normal resting pressure (41.5±8.6) mmHg and squeeze pressure (121.0±11.6) mmHg. All the patients were followed up to March 2018, and the median follow-up time was 4.5 months. Only 1 patient had supraclavicular lymph node metastasis and no local recurrence was found.
CONCLUSIONSThe safety of transanal total mesorectal excision for male patients with low rectal cancer and difficult pelvis is acceptable. TaTME is helpful to preserve the anal sphincter.
Adult ; Anal Canal ; surgery ; Humans ; Laparoscopy ; Male ; Neoplasm Recurrence, Local ; Pelvis ; surgery ; Postoperative Complications ; Rectal Neoplasms ; surgery ; Retrospective Studies ; Treatment Outcome ; Universities