BACKGROUND: Previous research has indicated that bone marrow-derived mesenchymal stem cells had immunological regulation and hematopoiesis role. There were significant differences in immunological regulation and hemopoietic function between patients with aplastic anemia and normal cases. OBJECTIVE: To summarize the in vitro biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells from patients with aplastic anemia.METHODS: A computer-based online search was conducted in PUMMED (1987-2009) and VIP database (1989-2009) with the key words of "mesenchymal stem cell, bone marrow" in both Chinese and English. There were 55 articles in total. Articles which were related to biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells were included; however, duplicated articles were excluded. Finally, 32 articles were included, containing 3 reviews in English, 23 original articles in English, and 6 original articles in Chinese. RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells had the capacities of high proliferation, self-renewal and multilineage differentiation; in addition they had the roles of supporting hematopoiesis, promoting implantation and hematopoietic reconstitution in vivo. Bone marrow-derived mesenchymal stem cells still had the effects of negative immune regulation and reducing the incidence of graft-versus-host disease (GVHD). Aplastic anemia correlated with hematopoietic stem cells in the pathogenesis of intrinsic defects in the proliferation or differentiation of hematopoietic microenvironment and immune system abnormalities and other related disorders. Bone marrow-derived mesenchymal stem cells of patients with aplastic anemia played an important role in the pathogenesis of this disease.

Objective] To investigate and compare with western medicine on the advantages of traditional Chinese medicine in the treatment of subacute thyroiditis. [Method] Select subacute thyroiditis clear diagnosis, single disease cases in clinical work.For patient treated with pure Chinese medicine internal and external treatment. Observation, comparison and analysis of the degree of symptom relief, the size of the side effect, the prognosis of patients and the satisfaction of patients with medical treatment.Discussion on Chinese medicine in the treatment of subacute thyroiditis the high compliance of advantages, the advantage of fewer side effects,therapeutic advantage and the advantage of improving the prognosis and reducing the sequela of disease. [Result] Through clinical observation and comparison, traditional Chinese medicine to western medicine in the treatment of subacute thyroiditis, good treatment compliance, curative effect are exact, side effects rarely, high patient satisfaction,good prognosis show obvious advantage. [Conclusion] Chinese medicine treatment of subacute thyroiditis, compared with the western medicine has obvious advantage for early clinical intervention, controling an illness as soon as possible, improving prognosis, reducing hypothyroidism incidence are of great significance.

Objective To analyze the risk factors related to obesity of preschool children. Methods The physical activity and eating behavior of 120 obese children aged 1～6 years were investigated with questionnaire by case control 1:1 matched. Results Obesity group spent significantly longer time to watch TV than control group, but the time for physical activity, feeding pattern during the first 4 months, the time of initial supplementary foods and the sleeping duration were not different between both groups. Conditioned logistic regression of multi-variables showed that good appetite, fast eating, overeating of carbohydrate and meat , and snacks before breakfast were the risk factors of child obesity. Conclusion The bad eating behaviors and the lack of physical activity were related to obesity in preschool children.

Objectives: To verify the feasibility and safety of the anterior transpedicular screw(ATPS) fixation of the upper thoracic spine (T1-T4) through the radiological anatomy study on the cadaveric specimens. Methods: The upper thoracic spine thin-section CT data of 40 cases were collected from the radiology de-partment′s database(20 males and 20 females, aged from 18 to 68 years, the mean age was 39.7 years). The data of OPW(outer pedicle width), OPH(outer pedicle height), PAL(pedicle axis length), TPA(transverse section angle), SPA(sagittal section angle), DTIP(distance of transverse intersection point) and DSIP(distance of sagittal intersection point) of each pedicle were measured on the transverse and sagittal sections through the axis of each pedicle. The data were recorded and statistically analyzed. 10 upper thoracic spine(C7-T6) specimens of adults(5 males and 5 females, with unknown ages), with no damage to their appearance, the costovertebral joints and paravertebral soft tissue were completely retained. Then simulate surgical operations were done on the cadaveric specimens based on the obtained data. Screws were implanted anteriorly by free hand. After that, the specimens accepted X-ray fluoroscopy and CT scan. At last, the screws were removed, the speci-mens were sawed along the transaction and sagittal section of the screw channel. Then the success rate of the screw placement was evaluated according to Rao′s worn out classification standard of pedicle screws. Results: From T1 to T4, the OPW decreased from 8.14mm to 3.47mm; the OPH increased from 6.89mm to 10.29mm; the TPA decreased from 32.96° to 11.64°; the DTIP increased from 1.80mm to 5.50mm; the SPA increased from 104.95° to 115.74°; the DSIP increased from 5.95 to 8.76mm; the PAL changed irregularly, from 32.95 to 35.96mm. The pedicle diameters of T3 and T4 were too small to implant ATPS, but the ARTPS can be implanted successfully. The diameter of ATPS was about 4.0mm; the length of ATPS was about 35mm. The diameter of ARTPS was about 5.0mm; the length of ARTPS was about 35mm. 80 pedicle screws were implanted anteriorly, according to Rao′s worn out classification standard of pedicle screws, the fine rate was 90%. The internal walls of 7 pedicles were broken by screws of less than 2mm and no compression to the spinal cord. The internal walls of 5 pedicles were broken of 2 to 4mm, 1 at T1, 1 at T3 and 3 at T4, with varying degrees of spinal cord compression. The internal walls of 2 pedicles were broken of greater than 4mm, 1 at T2 and 1 at T4, with serious spinal cord compression. The external wall of 1 pedicle was broken at T2. Conclusions: The ATPS techniques at T1, T2 and the ARTPS techniques at T3, T4 are feasible, but the safety and clinical practice and further research is needed.

Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.Methods R plasmids of pseudomonas aeruginosa were isolated from the sputum of elderly patients suffering from respiratory infection,and the elimination test of radix Pulsatillae extracts on these plasmids was performed in vitro and in vivo.Results Being treated with the extracts for 24,48 and 72 h in vitro,the elimination rate of R plasmids was 0,1.8 %and 3.4 %respectively,while in SDS(sodium dode canesulphonate)control group the elimination rate was 0,0.8 %and 1.6 %respectively.Being treated with the extracts for 24,48 and 72 h in vivo,the elimination rate was 0,2 %and 12.8 %respectively,and 0 in the control group.Conclusions Both in vitro and in vivo,radix Pulsatillae extracts have an elimination effect on R plasmid of pseudomonas aeruginosa,and the effect in vivo is stronger than that in vitro.

Brain-computer interface (BCI) system is a system that achieves communication and control among humans and computers and other electronic equipment with the electroencephalogram (EEG) signals. This paper describes the working theory of the wireless smart home system based on the BCI technology. We started to get the steady-state visual evoked potential (SSVEP) using the single chip microcomputer and the visual stimulation which composed by LED lamp to stimulate human eyes. Then, through building the power spectral transformation on the LabVIEW platform, we processed timely those EEG signals under different frequency stimulation so as to transfer them to different instructions. Those instructions could be received by the wireless transceiver equipment to control the household appliances and to achieve the intelligent control towards the specified devices. The experimental results showed that the correct rate for the 10 subjects reached 100%, and the control time of average single device was 4 seconds, thus this design could totally achieve the original purpose of smart home system.
Brain-Computer Interfaces ; Electroencephalography ; Evoked Potentials, Visual ; Humans ; Microcomputers ; Wireless Technology

OBJECTIVETo investigate the clinical efficacy of Panlongqi tablet (Chinese characters) combined with lumbar facet joint release for lumbar spinal stenosis of type Fengshi Bizu (Chinese characters).

METHODSSince February 2012 to February 2013, 120 patients with lumbar spinal stenosis of Fengshi Bizu (Chinese characters) syndrome were retrospectively studied. According to different treatment methods, 120 patients with lumbar spinal stenosis were divided into Panlongqi tablet (Chinese characters)group and control groups, respectively. In Panlongqi tablet (Chinese characters)group, 60 patients were treated by Panlongqi tablet (Chinese characters) combined with lumbar facet joints release solution including 26 males and 34 females with an average age of (60.40±3.36) years old ranging from 46 to 65 ; the course of the disease was 2 to 15 years (averaged 7.6 years). In control group the other 60 patients were treated with lumbar facet joint release including 24 males and 36 females with an average age of (61.20±2.47) years old ranging from 48 to 63; the course was 3 to 14 years (averaged 6.9 years). The clinical effect of patients were evaluated by JOA and ODI score before treatment, at 4 weeks and 3 months after treatment.

RESULTSAll patients were followed up for 4 to 7 months (means 5.6 months). After 3 months,7 cases in control group recurrenced symptoms,only 1 case in Panlongqi tablet (Chinese characters) group recurrenced. At 4 weeks and 3 months of follow-up, ODI score and JOA score of Panlongqi tablet group were much better than those of the control group.

CONCLUSIONFor lumbar spinal stenosis of type Fengshi Bizu (Chinese characters),which were treated with lumbar facet joint release with Panlongqi tablet(Chinese characters), supplemented by back muscle exercise, in relieving waist and low back pain symptoms and improving functional status of lower lumbar spine, can obtain satisfactory clinical outcome, is a good method of conservative treatment for such diseases.

Aged ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Exercise Therapy ; Female ; Humans ; Lumbosacral Region ; physiopathology ; Male ; Middle Aged ; Punctures ; Retrospective Studies ; Spinal Stenosis ; drug therapy ; physiopathology ; therapy

Objective To analyse the effect of antivirus treatment on the survival time of chronic severe hepatitis B retrospectively.Methods one hundred and twenty-one patients with chronic severe hepatitis B and positive HBV DNA were divided into 3 groups:42 patients in entecavir(ETV)group,34 patients in lamivudine(LVD)group and 45 patients in control group.The primary data were recorded and followed.The survival curve was described using Kaolain-Maier method.The comparison of survival rate among groups was done using Logrank test.Results The baseline characteristics were well balanced among 3 groups.When the follow-up ended,the mean survival time of ETV group,LVD group and control group was(49.4±5.8)weeks,(51.6±6.7)weeks,(32.8±5.7)weeks;the total survival rate was 0.567,0.557,0.318 respectively,there was a statistical difference between ETV group and control grouP(χ2=5.742,P<0.05),or between LVD group and control group (χ2=5.472,P<0.05).There was not statistical difference between ETV group and LVD group in the week of 2,4,8,12,24,48.Conclusion ETV and LVD can improve the survival rate of chronic severe heoatitis B,and ETV iS not different from LVD.

Objective:To establish an HPLC method for the determination of entrapment efficiency of matrine microspheres .Meth-ods:A Fortis XiC18 (250 mm ×4.6 mm, 5μm) column was used , the mobile phase consisted of methanol-acetonitrile-3%phosphoric acid solution (11∶80∶9).The flow rate was 0.6 ml· min-1.The detection wavelength was 210 nm, the column temperature was at 25℃and the sample size was 20 μl.Results: The linear range of matrine was 3-150 μg · ml-1 ( r =0.999 7).The recovery was 99.92%(RSD=0.97%, n=9).The entrapment efficiency of matrine microspheres was 81.85%±3.22%(n=3).Conclusion: The method is suitable for the determination of entrapment efficiency of matrine microspheres .

OBJECTIVE:To optimize water reflux extraction technology of Jiuwei yiwei mixture. METHODS:With the over-all score of the content of salvianoli acid B,a water-soluble component of the principal drug Salvia miltiorrhiza,the content of pae-oniflorin in Paeonia Radix Rubra and dry ointment yield rate as the observed index,and taking soaking time,extraction time, amount of water added,and extraction times as the factors,L9(34)orthogonal test was designed to optimize the extraction technolo-gy of Jiuwei yiwei mixture. And the verification test was conducted. RESULTS:The optimal technology was to add water with an amount 8 times as much as that of the drugs to soak them for 1 h and to conduct extraction twice,1 h for each. The overall scores of 3 batches of samples in the verification test were respectively 98.793,99.078 and 99.220(RSD<2%,n=3). CONCLUSIONS:The optimal technology for Jiuwei yiwei mixture is stable and feasible.