3.Microscopic observation on mycorrhiza of rare herb Dysosma versipellis.
Xiao-Ming TAN ; Li-Ying YU ; Ya-Qin ZHOU ; Xiao-Lei ZHOU ; Ying WEI
China Journal of Chinese Materia Medica 2013;38(23):4044-4046
Endophytic fungi played an important role in the growth of its host plant. To investigate the mycorrhizal characteristics and the distribution of fungi in the root, an endangered wild plant-Dysosma versipellis was collected and observed by electron microscope. The results showed that the host was closely associated with endophytic fungi. The fungi were mainly distributed in the epidermis and cortex. The aseptate and septate fungi with swollen hyphae were observed in some cell of the cortex. The result provides a reference for the study of mycorrhizal structure of Dysosma genus and the interaction between the fungi and its host.
Berberidaceae
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microbiology
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ultrastructure
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Endangered Species
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Endophytes
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physiology
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ultrastructure
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Fungi
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physiology
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ultrastructure
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Microscopy, Electron
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Plant Roots
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microbiology
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ultrastructure
4.Curative Effects of Monosialotetrahexosyl Ganglioside on Neonates with Moderate and Severe Hypoxic-Ischemic Encephalopathy
ya-dong, LU ; yong, LI ; xiao-yu, ZHOU ; xiao-ming, BEN
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To observe the curative effects of monosialotetrahexosyl ganglioside(GM1)on neonates with moderate and severe hypoxic-ischemic encephalopathy(HIE).Methods Eighty-six neonates with HIE were randomly divided into GM1 treatment group and control group.The control group(42 cases)were received routine treatment(including cerebrolysin and citicoline);the treatment group(44 cases)were given GM1 on the basis of routine treatment as early as possible(within 6 hours after birth).Brain CT,neonatal behavioral neurological assessment(NBNA)and children's development center of China(CDCC)at 12 months after birth were proformed in both groups.Results Brain CT,NBNA and CDCC markers in treatment group were better than those in control group(Pa
5.The protective effect on joint destruction of ~99Tc-MDP and its effect on tumor necrosis factor alpha in rat collagen-induced arthritis
Ya-Ou ZHOU ; Xiao-Xia ZUO ; Hui LUO ; Xian-Zhong XIAO ; Yi-Sha LI ;
Chinese Journal of Rheumatology 2001;0(05):-
Objective To determine the effects of~(99)Tc-MDP on joint inflammation and bone destruc- tion in collagen-induced arthritis(CIA)rats model and its effect on tumor necrosis factor alpha(TNF-?). Methods CIA was induced by immunization of male SD rats with an emulsion of collagen.~(99)Tc-MDP or placebo was intravenous infused to rats for 20 days.Joint inflammation was assessed by arthritis index.Lesions of bone were assessed based on the histological changes in ankle joints,radiographic analysis in hind paw with Larsen score.Systemic TNF-?level was measured by radioimmune assay.Results~(99)Tc-MDP suppressed joint swelling(P
6.Application of thenar perforator flaps for the narrow cicatricial contracture at thumb and the first web.
Xiao ZHOU ; Ya-Jun XU ; Yong-Jun RUI ; Yue-Feng BAO
Chinese Journal of Plastic Surgery 2013;29(3):181-183
OBJECTIVETo discuss the therapeutic effect of thenar perforator flaps for the narrow cicatricial contracture at thumb and the first web.
METHODSFrom Aug. 2010 to Jan. 2012, 9 cases with narrow cicatricial contracture at thumb and the first web, were treated. The defect size after releasing the contracture ranged from 8 mm x 20 mm to 15 cm x 30 mm. The bilateral thenar perforator flaps beside the wound were designed which size was 10 mm x 25 mm to 15 mm x 35 mm. The wounds at donor sites were closed directly.
RESULTSAll the 9 flaps survived completely with primary healing. The patients were followed up for 6-18 months. The flaps had soft texture and good appearance. The shape of flaps and function of the fingers were satisfied after 6-18 months of follow-up. There was no scar contracture at incisions in thenar. The thumb motion was really normal. The abduction of first web was 70 degrees - 90 degrees degrees.
CONCLUSIONSThe thenar perforator flaps is one of the ideal methods for the treatment of narrow cicatricial contracture at thumb and the first web. The main artery is not sacrified.
Arteries ; Cicatrix ; surgery ; Contracture ; surgery ; Fingers ; Humans ; Perforator Flap ; transplantation ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; Thumb ; surgery ; Wound Healing
8.Arthroscopic treatment of tibial intercondylar eminence fractures in children with single Kirschner wire and suture avoiding epiphyseal line fixation technique.
Qi-chun ZHAO ; Xiao-wen DENG ; Ping ZHOU ; Xi-fu SHANG ; Zhu YA-LIN
China Journal of Orthopaedics and Traumatology 2015;28(6):504-507
OBJECTIVETo discuss arthroscopic technique of single Kirschner wire and suture avoiding epiphyseal line fixation for tibial intercondylar eminence fracture and its clinical results.
METHODSFrom May 2008 to December 2012, 21 patients (13 males, 8 females, ranging in age from 6 to 14 years old) with tibial intercondylar eminence fracture were treated arthroscopically with single Kirschner wire and suture avoiding epiphyseal line fixation technique. According to Meyers and McKeever classification, 7 patients were type II, 10 patients were type III, and 4 patients were type IV. Active rehabilitation began at one week after operation. The patients were followed up for 10 to 30 months. X-ray films were taken to evaluate fracture healing at 1, 3, 6 months after operation; range of motion, the anterior drawer test, the lachman test and the Lysholm knee score were used to evaluate clinical effects.
RESULTSAll fractures were healed without displacement at 6 weeks after operation. Anterior drawer test and the lachman test were both negative in all patients at 3 months after operation. Lysholm knee score was 95.5 ±2.5 at 6 months after operation, and postoperative X-ray film did not find epiphyseal line broadening or narrowing.
CONCLUSIONArthroscopic treatment for tibial eminence intercondylar fracture with single Kirschner wire and 8-shaped suture avoiding epiphyseal line fixation technique has many advantages, such as firm fixation,early mobilization, less invasive, less injury of physis and satisfactory effect.
Adolescent ; Arthroscopy ; Bone Wires ; Child ; Female ; Fracture Fixation, Internal ; Humans ; Male ; Sutures ; Tibial Fractures ; surgery
9.Study of the treatment of ~(99)Tc-methylene diphosphonate on type Ⅱ collagen induced arthritis in rats and its effect on the synovial matrix metalloproteinase-3 and tissue inhibitor of matrix metalloproteinase-1
Yan-Li XIE ; Hui LUO ; Xiao-Xia ZUO ; Ya-Ou ZHOU ; Qiong FU ;
Chinese Journal of Rheumatology 2003;0(07):-
Objective To observe the treatment of ~(99)Te-MDP on typeⅡcollagen induced arthritis (CIA)in rat,and the effect on the expression of synovial MMP-3 and TIMP-1 mRNA.To explore the mech anisms of the ~(99)Te-MDP in the treatment of rheumatoid arthritis.Methods The rats in which C1A(n=24)were divided into three group:the control group(n=8),~(99)Tc-MDP group(n=8)and Methotrexate group(n=8). Arthritis were evaluated by arthritis index and histopathological index and the expressions of MMP-3 and TIMP-1 mRNA in synovium were detected by RT-PCR.Results①The arthritis indexs of the control group, the methotrexate group,the ~(99)Tc-MDP group were increased with time.②The histopathological scnres of the control group were significantly higher than those of methotrexate group and ~(99)Tc-MDP group(P<0.01).The histopathological scores of cartilage destruction and bone erosion of ~(99)Tc-MDP group were lower than those of methotrexate group(P<0.05).③The levels of MMP-3 mRNA of the control group,~(99)Tc-MDP group, methotrexate group were notably higher than those of the control group(P<0.01).The levels of control group was notably higher than that of the ~(99)Tc-MDP group(P<0.05).There was not significant difference in all groups on the levels of TIMP-1 mRNA(P>0.05).Conclusions ~(99)Tc-MDP can notably relieve the arthritis symdrome and retard the catilage damage and bone erosion of CIA in rats,and could significantly decrease the MMP-3 mRNA in the synovium.Which may be one of the therapeutic mechanism.~(99)Tc-MDP is better than methotrexate in retarding catilage and bone erosion and decreasing MMP-3 mRNA in CIA rats in a 3-week therapeutic intervention.
10.Clinical observation of the carotid of patients with anterior ischemic optic neuropathy using doppler ultrasound
Ya-Li, ZHANG ; Xiao-Li, CUI ; Xian-Min, JI ; Ai-Yi, ZHOU
International Eye Science 2015;(7):1269-1270
AlM: To investigate the relationship between the anterior ischemic optic neuropathy ( AlON ) and the carotid artery change using doppler ultrasound.METHODS:Fifty-four cases of AlON patients and 54 cases of healthy control were observed, atherosclerotic spots were detected by the application of color ultrasound.RESULTS:ln AlON group of 54 patients, 38 cases appeared carotid atherosclerosis, accounting for 70%. The number of cases with hard plaque, soft plaque and mixed plaques were 18, 13, and 7 respectively, accounting for 33%, 24% and 13%. ln the control group, 20 cases were detected atherosclerotic change, accounting for 37%. And the number of cases with hard plaque, soft plaque and mixed plaques were 12, 5 and 3 respectively, accounting for 22%, 9%, 6%. Significant stenosis and velocity change were showed in neither AlON group nor control group. Compared with the control group, AlON group had more cases of atherosclerotic plaque, the difference was statistically significant (χ2=12. 836, P=0. 005)CONCLUSlON: The incidence of AlON is correlated with carotid atherosclerosis, and carotid ultrasonography is significantly valuable for AlON etiology and diagnosis.