1.Obstructive sleep-disordered breathing in infants.
Xiao-hong CAI ; Liang-xing WANG
Chinese Journal of Pediatrics 2013;51(10):753-755
Continuous Positive Airway Pressure
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Facial Bones
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abnormalities
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Humans
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Infant
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Larynx
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pathology
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Oxygen Inhalation Therapy
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Pharynx
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pathology
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Polysomnography
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Respiration
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Risk Factors
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Sleep Apnea Syndromes
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diagnosis
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etiology
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therapy
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Sleep Apnea, Obstructive
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diagnosis
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etiology
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therapy
2.Human umbilical cord mesenchymal stem cells may differentiate into Leydig cells through conditioned medium induction.
Xiao-yu XING ; Ji-tao FAN ; Zhi-yuan ZHANG ; Liang ZHONG ; Jie SUN
National Journal of Andrology 2015;21(1):11-16
OBJECTIVETo explore the feasibility of inducing human umbilical cord mesenchymal stem cells (HuMSCs) to differentiate into Leydig cells through conditioned medium derived from Leydig cells.
METHODSHuMSCs and Leydig cells were obtained by tissue blocks culture attachment and enzymatic digestion respectively. HuMSCs were induced by conditioned medium of Leydig cells as an experiment group while those before induction were cultured as a control group. The expressions of LHR, 3β-HSD and StAR in the induced HuMSCs were determined by RT-PCR after 3, 7 and 10 days of culture; those of CYP11A1, CYP17A1 and 3β-HSD measured by immunofluorescence staining after 2 weeks; and that of 3β-HSD detected by Western blot after 4 weeks.
RESULTSThe experimental group showed positively expressed LHR, 3β-HSD and StAR at 3, 7 and 10 days, CYP11A1, CYP17A1 and 3β-HSD at 2 weeks, and 3β-HSD at 4 weeks, while the control group revealed negative expressions at all the time points.
CONCLUSIONInduced with conditioned culture medium derived from Leydig cells, HuMSCs are likely to differentiate into steroidogenic cells and eventually into Leydig cells.
Cell Differentiation ; Culture Media, Conditioned ; Humans ; Leydig Cells ; cytology ; Male ; Mesenchymal Stromal Cells ; cytology ; Umbilical Cord ; cytology
3.Application of gene knockout technology in spermatogenesis-associated gene study.
Xiao-Ju DING ; Chao-Liang WANG ; Wei-Xing ZHANG ; Rui WANG
National Journal of Andrology 2014;20(9):850-853
The gene approach to the pathogenesis of male infertility may bring about some strategies for the diagnosis and manage of the condition. Gene knockout technology is the mainstream method currently used in the study of gene function. Screening and identification of testis-specific genes and insights into their features and functions in spermatogenesis are significant for a further understanding of testicular functions and searching for new therapeutic targets for male reproductive disorders. This review focuses on the application of gene knockout technology in the study of spermatogenesis-associated genes.
Animals
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Gene Knockout Techniques
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Humans
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Infertility, Male
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genetics
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Male
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Spermatogenesis
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genetics
4.Analysis on hemorrhagic fever with renal syndrome through surveillance data from 1993 - 2004 in Donggang distrat, Rizhao city Shandong province.
Liang-Hong XING ; Xiao-Mei DENG
Chinese Journal of Epidemiology 2008;29(4):416-416
Adult
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Animals
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China
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epidemiology
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Female
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Hemorrhagic Fever with Renal Syndrome
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epidemiology
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virology
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Humans
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Male
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Middle Aged
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Rodentia
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virology
5.Quality Study on Cinnabar-analysis of Dyeing Status for Cinnabar
Xiao XING ; Zhimin LIU ; Tongtong XU ; Da GU ; Yubing LIANG ; Xin ZHANG ; Jianlong YANG
China Pharmacy 2016;27(27):3864-3866
OBJECTIVE:To analyze the dyeing status of cinnabar and its pieces,and provide reference for its quality clinical safetey appicaton. METHODS:TLC was used for the qualitative identification of amaranth,carmine,erythrosine,acid red 73, 808 udan and indirubin. HPLC-MS was used to detect the 808 udan :HPLC conditions were as follows,column was Acquity UPLC BEH C18 with mobile phase of cetonitrile-0.1% formic acid(70∶30,V/V)at a flow rate of 0.3 ml/min,the detection wave-length was 520 nm;MS conditions were as follows,ion source was electrospray ionization source,scanning mode was positive ion scanning with full scanning tandem mass spectrometry,nebulizer pressure was 30 psi,drying gas was nitrogen,ion spray voltage was 4 000 V,collision energy was 30 V,and the injection volume was 5 μl. The volumetric method was used for content determi-nation of HgS. RESULTS:TLC spots of amaranth,carmine,erythrosine,acid red 73,808 udan and indirubin were clear and well-separated. 4 batches of 808 udan dyeing were included in the 18 batches of samples,6 batches had non-compliance contents (including 3 batches of 808 udan dyeing). CONCLUSIONS:Dyeing-doped and other quality problems exist in the cinnabaris in markets,which should be noticed.
6.Identification and renaming of one strain used for tracheitis vaccine production
Jichun SHI ; Huijing DU ; Li LIANG ; Kang LI ; Xiao XU ; Yuhua XING ; Cuiping CHENG ; Qiang YE
Chinese Journal of Microbiology and Immunology 2016;36(6):448-452
Objective To identify and rename one strain stored in National Center for Medicine Culture Collections ( CMCC) and used for tracheitis vaccine production. Methods The test strain CMCC (B)29108 and the type strain DSM30007T were cultured on NA medium. Characteristics in morphology, physiology, biochemistry and fatty acid profile were compared between the two strains. Phylogenetic analysis was based on 16S rRNA and rpoB gene sequences, together with the DNA-DNA hybridization assay. Results A Comparative analysis of a partial sequence of the 16S rRNA gene sequence revealed that the CMCC( B) 29108 strain was closed to the Acinetobacter species and showed the highest similarity with the type strain Acinetobacter baumannii DSM30007T. Moreover, the CMCC(B)29108 strain was highly similar to type strain DSM30007T in morphology, physiology, biochemistry and fatty acid profile. On the phylogenetic tree based on 16S rRNA and rpoB gene of all Acinetobacter members, the CMCC(B)29108 strain steadily clustered into one independent branch only with the DSM30007 T strain with a DNA-DNA hybridization value of 100%. Conclusion The CMCC(B)29108 strain that is one of the strains used for the production of tracheitis vac-cine should be assigned to the species of Acinetobacter baumannii based on its phenotypic, phylogenetic and chemotaxonomic characteristics.
7.Comparative analysis of two surgical techniques for controlling nasal width after Le Fort I osteotomy.
Miao-Zhen WANG ; Xiao-Xia WANG ; Zi-Li LI ; Biao Y I ; Cheng LIANG ; Xing WANG
Chinese Journal of Plastic Surgery 2013;29(3):184-188
OBJECTIVETo compare the efficacy of two surgical techniques for controllong nasal width after Le Fort I osteotomy.
METHODSFifty-five patients who received the Le Fort I osteotomy have been included in this study. They were randomly divided into 2 groups. The experimental group received extraoral ABS, and the control group received traditional intraoral ABS. 3D photos of the patient's face were taken before operation and at postoperative 3 months. Alar width was measured on the 3D photos. Data was reported as means and standard deviations, and statistic analysis was done by using student t test.
RESULTSCompared with presurgical data, G. lat-G. lat increased by (2.66 +/- 1.47) mm, Al-Al increased by (2.20 +/- 1.22) mm and Sbal-Sbal increased by (1.30 +/- 1.33) mm in experimental group. G. lat-G. lat increased by (1.38 +/- 1.29) mm, Al-Al increased by (1.06 +/- 0.95) mm and Sbal-Sbal increased by (0.36 +/- 1.33) mm in the control group. There was significant difference between two groups.
CONCLUSIONSThe surgical technique of ABS is the most important factor for determining the postoperative alar width. Both techniques have better effect on the Sbal-Sbal width control than the G. lat-G. lat and Al-Al width control. Traditional intraoral ABS can more effectively control the alar width. Both techniques cannot completely control the alar base widening after Le Fort I osteotomy.
Face ; Humans ; Nose ; anatomy & histology ; Nose Deformities, Acquired ; surgery ; Osteotomy, Le Fort ; adverse effects ; Photography
8.Clinicopathological analysis of 34 cases of primary small intestine lymphoma
jun-liang, YAO ; xing-zhi, NI ; yan-ying, SHEN ; zi-zhen, ZHANG ; zi-li, XIAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To analyse the clinicopathological features of primary small intestine lymphoma(PSIL), and explore the relationship between clinical stage,histological findings,therapeutic modality and prognosis. Methods The clinical data of 34 cases of PSIL were collected,the pathohistological features and results of immunohistochemical examinations were obtained,and the follow-up findings were adopted for comprehensive analysis. Results Among these 34 cases of PSIL,abdominal pain or discomfort,gastrointestinal bleeding and abdominal mass were the predominant symptoms.PSIL mainly involved ileum,especially the bottom of ileum and ileocecal area.Among the 26 patients with follow-up for more than one year,the 1-year survival rate was significantly higher in patients without tumor perforation than those with tumor perforation(76.2% vs 20.0%)(P
9.On expression of VEGF-C and its receptor in breast carcinoma tissue and in peritumoral tissue and their clinic significance
Xiao-Jun MA ; Yong-Dong PU ; Jian-Miao HE ; Liang-Hong NI ; Hong-Xing JIA ;
Cancer Research and Clinic 1997;0(03):-
Objective To study the expression of VEGF-C and its receptor in breast carcinoma tissue and in peritumoral tissue,as well as their clinic significance.Methods Immunohistochemistry SP method was used to examine the expression of VEGF-C and VEGFR3 in 70 cases of breast cancer and in its peritu- moral tissue.Results In all 70 cases of breast cancer,the positive expression rate of VEGF-C in breast car- cinoma tissue was 78.6 %,and its rate in peritumoral tissue was 54.3 %.There was a significant stastistic dif- ference between the two groups(P
10.The study of catheterization to measure ventricular pressure in mice through venous cannula in external jugular vein.
Pan-Pan LIU ; Shuang-Quan YAN ; Ma-Yun CHEN ; Li-Zhen ZOU ; Xiao-Ying HUANG ; Liang-Xing WANG
Chinese Journal of Applied Physiology 2014;30(2):189-192
OBJECTIVETo study a feasible method of measuring right ventricular pressure by catheterization in mice.
METHODSMeasuring the right ventricular pressure and the pulmonary artery pressure by homemade PE pipe through venous cannula in external jugular vein, using catheterization in mice with powerlab multimodal biometric signal recording system.
RESULTSForty-six out of 51 mice were experimented with this method smoothly and got a total success rate of 90.2%. Thirty of 33 normal mice and 16 of 18 mice with pulmonary arterial hypertension (PAH) were catheterized successfully. The right ventricular pressure were as follow: systolic blood pressure: (23.4 +/- 5.7) mmHg in normal group vs (32.2 +/- 2.8) mmHg in mice with PAH, diastolic blood pressure: (3.7 +/- 2.6) mmHg vs (3.8 +/- 2.0) mmHg, mean pressure: (12.0 +/- 3.7) mmHg vs (14.9 +/- 2.3) mmHg. After autopsy for those 5 failed cases, we found that 2 cases were into the inferior vena cava, another 2 cases pierced the right auricle and the last one punctured the axillary vein into the chest wall.
CONCLUSIONMeasuring the right ventricular pressure through venous cannula in external jugular vein with homemade PE pipe in mice gets not only a high success rate but also help to save time. Moreover, this method can be popularized easily. It is a good and feasible method for measuring right ventricular pressure in mice.
Animals ; Cardiac Catheterization ; methods ; Jugular Veins ; Male ; Mice ; Mice, Inbred BALB C ; Ventricular Pressure