Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Male ; Middle Aged ; Purpura, Thrombotic Thrombocytopenic ; etiology ; Transplantation, Autologous

Objective To assess the clinical value of 18F -FDG PET/CT in the diagnosis of primary, suspected recurrent and metastatic cervical cancer.Methods The results of 67 women who were suspected suffering from cervical cancer had undergone Color dopplar ultrasound,HPV,TCT,some of them do CT,MRI and SCC -Ag examination from April 2013 to April 2015 were retrospectively analyzed.Their 18F -FDG PET/CT images with the other test results were compared.The presence or absence of tumor was confirmed by histopathology.Results In the 67 patients,22 cases were finally diagnosed as primary cervical tumors,and 22 cases of recurrent and metastatic cervical tumors.In all the patients with cervical cancer,18F -FDG PET/CT found a total of 41 cases,which had contributed to the diagnosis in 94.0%,But 3 cases had not been found.For the detection of primary cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 4.2%,For the detection of recurrent and metastatic cervical tumors, 18F -FDG PET/CT was diagnostically wrong in 7.0%,and 2 cervical adenocarcinoma patients were not diagnosed by 18F -FDG PET/CT.Conclusion The study showed a significantly higher sensitivity of 18F -FDG PET/CT for the detection of primary,recurrent and metastatic cervical cancer than the other auxiliary examination.18F -FDG PET/CT for the diagnosis of cervical cancer can be worth popularization.

OBJECTIVE To compare the treatment effects of secreory otitis media with artificial eustachian tube insertion and tympanotomy tube insertion.To provide useful information for the treatment of secretory otitis media.METHODS Seventy-one patients(86 ears) with secretory otitis media were randomly divided into tow groups:experimental group(36 cases,44 ears) and control group(35 cases 42 ears).The experimental group was treated by artificial eustachian tube insertion under nasal endoscope.Drugs were injected through the tube into the tympanic cavity everyday after flushing it. The control group were treated by traditional method—the tympanic ventilation tube.All patients were followed up for 6 months.RESULTS The experimental group: 21 ears(47.73%) were cured,19 ears(43.18%) were straightened up,and no effects observed in 4 ears (9.09%).The control group:12 ears(28.57%) cured,17 ears(40.48%) wered straightened up and no effects obsered in 13 ears(30.95%).The efficiency rate of the experimental group(90.91%) was much higher than the control group(69.05%,P

Objective To explore the effects of oxidative stress in the pathogenesis of lung injury and observe the influence of N-acetylcysteine (NAC). Methods The lung histopathology was observed by light microscope. The level of 8-iso-prostaglandin F2?lpha (8-iso-PGF2?) in blood plasm were measured by ELISA. The difference of 8-iso-PGF2? in blood plasm in air group, different dose NAC groups between hyperoxia-model and the air group was compared. Results In hyperoxia-model group, the inequality of size of lung alveoli, hemorrhage and inflammatory cell infiltration in lung alveoli were observed on the 3rd and 7th day. The alveolar septum was thick in the hyperoxic-damaged lungs on the 14th and the 21st day. In hyperoxia+high-dose NAC group, very small amounts of red blood cells leaked out into alveoli on the 3rd and 7th day and alveolar septum had no thickening obviously on the 14th day and the 21st day. The level of 8-iso-PGF2? in blood plasm in hyperoxia-model group [(28.33?5.57) pg/ml, (51.21?15.01) pg/ml, (84.54?14.85) pg/ml and (43.14?11.37) pg/ml at every time points] was higher than that of the air group and hyperoxia+high-dose NAC group(P

Adult ; Chordoma ; Humans ; Male ; Nasal Septum ; pathology ; Nose Neoplasms

Abstract: Objective　To screening new compounds that can inhibit the growth and biofilm formation of Staphylococcus aureus. Methods　Compounds that can inhibit the growth of Staphylococcus aureus were screened from the FDA approved drug library by 96 well plates. The absorbance value of 600 nm wavelength (OD600) was measured by Microplate Reader to detect the growth of Staphylococcus aureus planktonic cells in the culture supernatant. The minimum inhibitory concentration (MIC) of ozanimod against Staphylococcus aureus clinical isolates were detected by micro broth dilution method. The inhibitory effect of sub-inhibitory concentrations of ozanimod on the biofilm formation of Staphylococcus aureus was detected by crystal violet staining. Results　This study found that ozanimod could significantly inhibit the growth of Staphylococcus aureus SA113 (screening reference strain), and the MIC was 25.00 μmol/L. The MIC of ozanimod against 119 clinical isolates of Staphylococcus aureus [65 isolates of methicillin sensitive (MSSA) and 54 isolates of methicillin resistant (MRSA)] was 12.50 or 25.00 μmol/L. The MIC50 and MIC90 of ozanimod against the 119 Staphylococcus aureus isolates all were 25.00 μmol/L. This study found that 6.25, 12.50, 25.00 μmol/L of ozanimod could significantly inhibit the biofilm formation of 2 MSSA and 2 MRSA. The sub-MIC concentration of ozanimod (12.50 μmol/L) could significantly inhibit the biofilm formation of 14 MSSA and 11 MRSA, but had no inhibitory effect on the growth of planktonic cells of these Staphylococcus aureus isolates. Conclusion　Ozanimod can inhibit the growth of Staphylococcus aureus, including MRSA, and has good antibacterial activity. The sub-MIC concentration of ozanimod could significantly inhibit the biofilm formation of Staphylococcus aureus.

Adult ; Chondroblastoma ; pathology ; Chondroma ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Ovarian Neoplasms ; pathology ; surgery ; Ovary ; pathology ; Teratoma ; pathology

Objective To provide clinical evidence for the optimal acupuncture time in acupuncture treatment of hearing disorder in kids with cerebral palsy.Method Ninety eligible patients were randomized into 3 groups, 30 in each group. Group A was treated for 30 min, group B for 45 min, and group C for 1 h. The hearing improvement was evaluated by using brain-stem auditory evoked potential (BAEP).Result The detection of BAEP showed that the latent and peak periods of wave I,Ⅲ, and V were significantly shortened in the 3 groups after intervention (P<0.01), suggesting that acupuncture can produce a marked efficacy in treating hearing disorder in cerebral palsy. The inter-group comparisons showed that the efficacy of group A was significantly higher than that of group B and C (P<0.05); there was no significant difference in comparing the efficacy between group B and C (P>0.05). It’s indicated that the optimal acupuncture time span should be 30 min.Conclusion With the same acupuncture skills, acupuncture for 30 min can produce a comparatively higher efficacy in treating infantile hearing disorder.

Heart sounds teletransmission is very important in telemedicine.The heart sounds are transmitted to the server which has been accessed to internet in custody center based on GPRS,then doctors analyze the heart sounds and transmit the conclusion to the consumer.

Objective To develope both qualitative and quantitative analytic method of the four urinary markers,i.e.lactate,uracil,orotate and hippurate,from ornithine transcarbamylase deficiency(OTCD) by Gas Chromatography-Mass Spectrometry(GC-MS).Methods Urea in urine samples was decomposed with urease,and heptadecanoiate was added as internal standard,then protein was denatured with ethanol and removed by centrifugation.After evaporation,the residue was derivatized trimethylsilylly by BSTFA/TMCS,and analyzed by GC-MS.ResultsThe markers can be separated in total ion current profiles,with indentifications confirmed by mass spectra.The significantly elevated levels of lactate,uracil and orotate in urine from OTCD patient were droped to normal or subnormal levels,together with large amount of hippurate excretion in the urine,after clinical therapeutic measures,including introduction of benzoic acid,were performed.Conclusion GC-MS analysis of the urinary markers is a valuable tool for the diagnosis and evaluation of the therapeutic outcome of OTCD.