1.Application of RNA Secondary Structure in Phylogenetic Analysis of Microbiology
Yang LIU ; Xiao-Long CUI ; Wen-Jun LI ; Qian PENG ;
Microbiology 1992;0(02):-
Attention was gradually paid by biologists to the using of RNA secondary structure in the classification of microbiology and phylogenetic relationship analysis in recent years. The development around the research was summarized here briefly. And more emphasis was given to the part introducing the application of RNA secondary structure to the analysis of phylogenetic relationship.
2.lnfluence factors of dry eye among aerospace science and technology staff and the health guidance
Qian-Wen, LI ; Xiao-Lin, HAO ; Zhong-Chen, ZHANG
International Eye Science 2014;(12):2240-2243
AlM: To investigate and evaluate the prevalence of xerophthalmia among aerospace science and technology staff and its influencing factors for the eye health guidance.
METHODS: The staff underwent physical examination in our examination center from September 2013 to June 2014 were preliminary screened, and those patients with symptoms of dry eye were investigated with questionnaires and diagnosed by basic lacrimal secretion test ( Schirmer l ) , breakup time of tear film ( BUT ) test and fluorescein ( FL) staining test .
RESULTS: There were 606 complaints of dry eye symptoms of the 1 000 premiers, distributed in random (60. 6%), and 432 employees were diagnosed (43. 2%). The positive rate of dry eye was 71. 3%. The causes of xerophthalmia, besides of gender, age, also include a variety of factors such as air-conditioner, heating, video terminals, sleep time, etc.
CONCLUSlON: Dry eye, an epidemic disease, has a variety of symptoms and complex etiology, the main causes of aerospace science and technology staff with dry eye is working environment and some local factors. We can give appropriate health guidance according to the different factors. Meanwhile the relevant series of dry eye screening should also be taken as a routine examination in ophthalmic examination.
3.Rupture of primary splenic multilocular mucous cyst accompanied by pseudomyxoma peritonei: report of a case.
Wen QI ; Wen-xiu LIN ; Na DU ; Xiao WANG ; Li-yi GU ; Qian-xing ZHANG
Chinese Journal of Pathology 2013;42(2):129-130
Aged
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CDX2 Transcription Factor
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Cysts
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Homeodomain Proteins
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metabolism
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Humans
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Keratin-20
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metabolism
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Mucous Membrane
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pathology
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Peritoneal Neoplasms
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metabolism
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pathology
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surgery
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Pseudomyxoma Peritonei
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metabolism
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pathology
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surgery
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Rupture
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Splenic Neoplasms
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metabolism
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pathology
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surgery
4.Efficacy and safety of chimeric antigen receptor-T cells in the treatment of B cell lymphoma: a systematic review and meta-analysis
Zheng XIAO-HONG ; Zhang XI-YOU ; Dong QIAN-QIAN ; Chen FENG ; Yang SHOU-BO ; Li WEN-BIN
Chinese Medical Journal 2020;133(1):74-85
Background:Conventional treatment has limited efficacy in relapsed/refractory B-cell lymphoma.Since chimeric antigen receptor Tcell (CAR-T) technology has shown high safety and results in high remission rates,we investigated its efficacy and safety in B-cell lymphoma treatment and analyzed potential affecting factors to provide evidence for therapeutic strategies and applications.Methods:We searched databases including PubMed,Embase,and Cochrane up to July 2019.Meta-analysis 1 was conducted to study the efficacy of CAR-T cell for treating B-cell lymphoma,measuring the response rate and complete remission rate as outcomes.Sub-group analysis was performed for age,pathological type,target antigen,co-stimulatory molecule,and conditioning chemotherapy.Meta-analysis 2 was undertaken on the safety of the treatment with the incidence rate of toxicity (cytokine-releasing syndrome [CRS],neurotoxicity) as an outcome.Results:Seventeen studies were included in the systematic review and meta-analysis.It was found that CAR-T cells had good therapeutic effects in the following cases:B-cell lymphoma (patients ≥65 years old);diffuse large B-cell lymphoma pathological type;patients with treatment target antigen other than CD 19;patients treated with co-stimulatory molecules other than CD28,including 4-1BB+CD28 or 4-1BB;and patients treated with cyclophosphamide/fludarabine pre-treatment protocol conditioning chemotherapy.Although the CRS and neurotoxicity incidences were high,most were reversible with minimal risk of death.Conclusion:CAR-T cell treatment is safe for clinical annlication:however,toxicity effects should be monitored.
5.A new flavonoid glycoside from leaves of Eucalyptus robusta.
Xi-feng GUAN ; Qian-yi GUO ; Xiao-jun HUANG ; Ying WANG ; Wen-cai YE
China Journal of Chinese Materia Medica 2015;40(24):4868-4872
A new flavonoid glycoside, (-)-2S-8-methyl-5,7,4'-trihydroxyflavanone-7-O-β-D-glucopyranoside (1), along with five known ones, quercetin-3-O-(2"-galloyl)-α-L-arabinoside (2), kaempferol-3-O-α-L-arabinoside (3), guaijaverin (4), trifolin (5) and hyperin (6), was isolated from the leaves of Eucalyptus robusta. Their structures with absolute configurations were elucidated by NMR, HR-ESI-MS, CD spectra data and physicochemical methods. In addition, 2-6 were isolated from E. robusta for the first time.
Eucalyptus
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chemistry
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Flavonoids
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chemistry
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isolation & purification
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Glycosides
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chemistry
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isolation & purification
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Plant Leaves
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chemistry
6.Antimicrobial susceptibility of 487 Mycoplasma strains
Xiao-Yan QIU ; Wen-Zhong WANG ; Qian-Jun FU ; Shi-E CHEN ; Zhi-Xiong WANG ;
Chinese Journal of Infection and Chemotherapy 2006;0(04):-
Objective To analyze the antimicrobial susceptibility of Mycoplasma isolates for rational antimicrobial therapy. Methods BioM?rieux IST kit was used for identification and susceptibility testing of Mycoplasma strains.Results Mycoplasma was positive in 49.5% of the specimens tested.Of the Mycoplasma detected,Ureaplasma urealyticum(Uu)alone accounted for 74.7%,Mycoplasma huminis(Mh)alone accounted for 18.1%,and Uu+Mh was identified in 7.2% of the patients.The results of antimicrobial susceptibility testing showed that the Mycoplasma isolates were most susceptible to doxycycline (98.1%).Ciprofloxacin was the least active (17.3%).Conclusions Doxyeycline,josamycin,and clarithromycin can be used in the treatment of urinary tract infections caused by Mycoplasma.
7.Correlative analysis of eccentricity of optical treatment zone with tear film function and corneal shape after wearing orthokeratology lenses
International Eye Science 2018;18(11):1967-1972
AIM: To explore the correlation between eccentricity in the optical treatment area and tear film function and corneal morphology after orthokeratology.
METHODS:Eighty patients(80 eyes)with myopia who were treated with orthokeratology were selected as subjects. The eccentric distance, tear film function and corneal morphology of the patient's optical treatment area were detected at different time points before and after wearing the lens. According to the median eccentric distance, the patients were divided into low eccentricity group and high eccentricity group. The differences of tear film function and corneal morphology at different time points before and after wearing were compared between the two groups. Pearson correlation coefficient was used to analyze the correlation between total eccentricity and tear film function and corneal morphological parameters at 12mo after wearing glasses.
RESULTS:Compared with before wearing lenses, the corneal surface asymmetry index and corneal surface regularity index of the two groups were significantly increased at 1wk, 3, 6 and 12mo after wearing lenses(P<0.05). Patients in the high eccentric group were more significantly elevated than in the lower eccentric group, and the differences were statistically significant(P<0.05). The time of tear film rupture, corneal curvature, basic tear secretion, and central corneal thickness were significantly lower, and the differences were statistically significant(P<0.05). Compared with the patients with lower eccentricity, the patients with high eccentricity group had more significant reduction in tear film rupture time and basic tear secretion, and the difference was statistically significant(P<0.05), however, the degree of corneal curvature and central corneal thickness decreased less(P<0.05). There was no significant difference in corneal endothelial cell density between 1wk after wearing lenses and 3mo after wearing lenses(P>0.05). The difference was statistically significant at 6mo and 12mo after wearing lenses(P<0.05). At 12mo after wearing the lens, the total eccentric distance was positively correlated with the asymmetry index of the corneal surface and the regularity index of the corneal surface(r=0.869, 0.815; P<0.05). At 12mo after wearing the lens, the total eccentric distance was negatively correlated with tear film rupture time, corneal curvature, basic tear secretion, and central corneal thickness(r=-0.865, -0.745, -0.912, -0.713; all P<0.05), and there was no significant correlation with corneal endothelial cell density(r=0.185, P=0.199).
CONCLUSION:The eccentricity of the optical treatment area occurs at the beginning of wearing lenses. And the total eccentric distance has a significant correlation with the changes of tear film function and corneal morphology after keratoplasty.
8.Expression of IL-18 in the peripheral blood mononuclear cells of chronic hepatitis B patients
Weiqun WEN ; Lian ZHANG ; Zhangyong HU ; Mingxia ZHANG ; Hong XIAO ; Yi QIAN
Immunological Journal 2001;(2):125-127
Objective To investigated the possible roles of IL-18 in HBV infection, and the expression of IL-18 in the peripheral blood mononuclear cells (PBMC) of chronic hepatitis B. Methods In 15 cases of asymptomatic carriers, 30 cases of active and remissive phases of chronic hepatitis and 10 healthy individuals (as normal controls), IL-18 expression in PBMC were quantitatively analyzed with flow cytometric immunological method. The PBMC were separated routinely and stimulated with LPS (SIGMA) and Monensin (SIGMA) for 6 hours. Then the cells were harvested and fixed by PBS/4% paraformaldehyde. The treated cells were stored in liquid nitrogen for detection later. After immunologic stain, the expression of IL-18 in PBMC were examined by flow cytometry. Results ① IL-18 was the lowest in asymptomatic carriers. It was fewer in remissive phase than in normal controls (P<0.01).There was no difference between active phase and normal controls(P=0.25).②Within the group of active phase, IL-18 was significantly different between each grade of inflammatory activity(P<0.01),and correlated with serum ALT positively(r=0.63,P<0.01). Conclusion IL-18 may relate to disease activity and the inflammation of liver.
9.Evaluation the adenoid and tonsil in children with obstructive sleep apnea hypopnea syndrome
Qian CAI ; Zhenzhong SU ; Weiping WEN ; Liping CHAI ; Yiqing ZHENG ; Yishu TENG ; Xuan WU ; Jiqian XIAO
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(07):-
0.05),but there was a significant difference in the lowest SaO2,average SaO2 and AHI between the 2 groups(P
10.Effect of femtosecond laser and mechanical lamellar LASIK on the stability of corneal flap thickness
International Eye Science 2019;19(6):920-923
AIM: To explore the effects of femtosecond laser and mechanical lamellar blade excision laser in situ keratomileusis(LASIK)on corneal flap thickness stability and dry eye syndrome.
METHODS: Retrospective analysis of 80 patients with myopia who underwent LASIK in our hospital. According to different methods of flapping, it is divided into femtosecond laser group and mechanical laminating knife group. The flap thickness of the two groups of patients at different time points(1h, 1d, 1wk, 1mo, 3mo)were measured. The differences in corneal apex 1, 2, 3mm and corneal apex flap thickness in different directions were compared. The incidence of dry eye syndrome was compared between 1 and 3mo, 1a, 2a after surgery.
RESULTS: The apex thickness of the corneal flap of the femtosecond laser group is 99.62±4.50μm, and the thickness of the apex of the mechanical lamellar knife group is 125.25±12.81μm.The thickness of the corneal flap at different centrifugal points of the femtosecond laser flap is relatively uniform, while the thickness of the corneal flap of the mechanical lamellar knife varies greatly. The OSDI scores of the mechanical lamellar group were higher than those of the femtosecond laser group at 1, 3, 1, and 2a postoperatively(P<0.01). At 2a of follow-up, the incidence of dry eye in the femtosecond laser group was lower than that in the mechanical lamellar group(χ2=4.692, P=0.030). At 1 and 2a postoperatively, the proportion of mild dry eye in the femtosecond laser group was higher than that in the mechanical lamellar group(P=0.044, 0.001).
CONCLUSION: Compared with the mechanical lamellar scalpel, the corneal flap thickness of patients with femtosecond laser LASIK is better, more uniform and less error. And the incidence of dry eye after surgery is lower, with mild dry eyes.