Bone marrow mesenchyma stem cells are stem cells that occupy self-renewal and multiple differentiations into cells,which have been recognized as the optimal origin for liver cell.With an increasing number of studies addressing marrow mesenchyma stem cells,more attention has been drawn to treating liver disease using the stem cell and organ reconstruction,especially seeks for the appropriate in vitro induction system.This paper comprehensive summarized the differentiation characteristics of marrow mesenchyma stem cell under many kinds of micro environment,and explains the superiority,as well as existence question of each kind of induction condition.

Adolescent ; Child ; Diabetes Mellitus ; drug therapy ; Drug Design ; Humans ; Hypoglycemic Agents ; administration & dosage ; therapeutic use ; Insulin ; administration & dosage ; therapeutic use

Adolescent ; Child ; Diabetes Mellitus ; drug therapy ; Humans ; Hypoglycemic Agents ; pharmacokinetics ; therapeutic use ; Insulin, Short-Acting ; pharmacokinetics ; therapeutic use

[Objective]To introduce a method to obtain Schwann cells from newly born SD rats massively and purely by immunomagnetic heads method.[Method]SD rats that had been born within 5 to 7 days were used. Their bilateral sciatic nerves were dissected under sterile condition. Under 16?microscope the nerve fascicles and the epineurium were carefully extracted in oder to get the nerve tract without impurity cell. Then the nerve tract was cut into small particle about 1 mm3.Two enzymes (0.25% trypsinase and 0.2% collagenaseⅠ)were used to digest the particle of sciatic nerve specimens twice. After using 20% fetal bovine serum to stop the process of digestion,centrifugate(1000r/min,5 min) and DF12 culture medium was added into the precipitation.Seven days later,Schwann cells were purified with immunomagnetic heads.During the whole process,the change of Schwann cells was observed under the inverted biological microscop and vital force of Schwann cells was evaluated.The growth curve of Schwann cell was drawn with MTT.The Schwann cells was identicated under immunofluorescent test and record the purity.[Result]The cell separated from the SD rat's sciatic nerve and cultured in incubator was affirmed as Schwann cells.Immunomagnetic heads can be used to purify the Schwann cells.The 96% vigor and 98% pure Schwann cell cultures were generated and passaged after two days.[Conclusion]This method can obtain massive purified normal schwann cells to satisfy the need of tissue-engineered bioartificial nerve graft.

[Objective] To investigate the combined effects of different neurotrophin couples of NGF,bFGF,BDNF on rat spinal cord neurons.[Method]Spinal cord neurons were obtained from SD rats born within 1d,and then were seeded in culture plates.Different factors and their couples were added in each chamber while DMEM/F12 served as controls,concentration of NGF,bFGF or BDNF were 50ng/ml.Phase contrast microscope observation was done.At the 3rd and 7th day after incubation,the cells were detected by ?-tubulin3 immunofluorescence and Hoechst staining.The length of neuritis was measured,and the numbers of neuron cells and nuclears were determined.At the 1st,3rd,5th,7th and 9th day MTT method was used,and the growth curve was made according to OD results.[Result] The length of axon and positive rate in the experimental groups were superior to those in the control group(P

Objective To explore the potential risk factors of schistosomiasis transmission so as to provide evidence for setting up the surveillance scheme of schistosomiasis prevention and control. Methods The schistosome infection of local inhabitants, mobile population and stock from endemic areas were investigated from 2002 to 2007 by using IHA or ELISA technique. The historical endemic reports of Chongqing City and the schistosomiasis cases, and the plants imported from schistosomiasis endemic areas and Oncomelania snails were investigated. The behavior of local inhabitants was also investigated. Results The investigated people included 5 112 local inhabitants, 1 455 mobile people from schistosomiasis endemic areas and 1 744 residents returned from the endemic areas. The sero-positive rates of them were 0.57%, 1.44% and 0.86%, respectively. Eighteen cases of imported schistosomiasis were found. There were many plants and stock imported from the schistosomiasis endemic areas. No imported snail was found. The farming of local inhabitants depended on the cattle chiefly. The rate of the harmless lavatories was 14.88%. The local residents had the habit of water contact. Conclusion There are potential risk factors for schistosomiasis transmission in the Three Gorges Reservoir areas. The local residents have potential risk behavior of getting infection of Schistosoma japonicum. The surveillance should be emphasized on the mobile population, imported plants and stock from endemic areas.

The cardiovascular disease, accompanied by psychological disorder frequently, often re-quiresphysical and mental (double heart) treatment. The clinical teaching of cardiovascular graduate stu-dents is very important for cultivating qualified cardiovascular doctors. According to present situation, we improved the diagnosis rate and the doctor-patient communication skills by the strengthening of learning thedouble heart theory, the analysis ofdouble heart typical cases, and the clinical practice of double heart medical model. Accordingly, we enhanced the consciousness of double heart, which is helpful to reducing the conflicts between doctors and patients, promoted the rational use of medical resources, and fi-nally promoted the application ofdouble heartmedical model in clinical practice.

Acute Coronary Syndrome ; blood ; Aged ; Cell Membrane ; metabolism ; Female ; Humans ; Male ; Middle Aged ; Thromboplastin ; metabolism

AIM: To discover specific neural stem cell (NSC) proliferation-promoting factor, which will contribute to study on development of nervous system and treatment of nervous system diseases. METHODS: The extracts of forebrain, midbrain,afterbrain and cerebellum of neonatal mice were prepared, and the NSCs of newborn mice were cultured in vitro. Neurospheres were observed, immunocytochemical staining of characteristic protein, nestin, and MTT assay were performed to identify NSCs and their proliferative properties. RESULTS: A great deal of neurospheres were formed in the presence of the extracts of afterbrain and cerebellum, which were positive for characteristic protein (Nestin) of NSC showed by immunocytochemical staining. CONCLUSION: The afterbrain and cerebellum extracts can increase the total number of NSCs isolated from newborn mice in vitro in a dose-dependent manner.

Objective To analyze the ABCD1 gene mutations in 5 cases of X-linked adrenoleukodystrophy(X-ALD) patients and 2 cases of their mothers.Methods Of 5 patients with X-ALD,10 exons and flanking intronic sequences of ABCD1 gene were amplified by polyme-rase chain reaction,and then sequenced directly.The outcomes were compared with normal ABCD1 sequencings to identify the mutation type and site.Thirty normal men were examined in the mean time as control for the confirmation of mutations and gene polymorphisms.Results Three patients showed ABCD1 gene mutations,1 had a point mutation in exon 6,Arg518Gly(CGG→GGG);2 patients showed the same novel mutation in exon 1 with 8 bases deletion(134del8).Four gene polymorphisms were identified in exon 7.They were Gly551X(GGC→GGT),Arg554His(CGT→CAT),Gln567Arg(CAA→CGA) and Val582Ile(GTC→ATC).ABCD1 gene mutation was not found in 2 mothers from 2 unrelated fa-milies with X-ALD.Conclusions Three cases of 5 were detected for ABCD1 gene mutations.Between them,the 134del8 mutation is a novel one.Four new gene polymorphisms were detected in exon 7 in normal Chinese people,which were Gly551X,Arg554His,Gln567Arg and Val582Ile.