1.Synthesis and surface modification of magnetic silica micro-spheres and Its application in biomedicine
Tao HAN ; Qingping XIAO ; Zhongrong SONG
International Journal of Biomedical Engineering 2010;33(2):125-封3
Magnetic silica micro-spheres is a kind of novel functional materials, which is expected to be applied to targeted drug delivery, immunomagnetic beads, separation of nucleic acid and protein, immobilization of enzymes and isolation and purification of natural products in biomedicine. This review describes the methods of synthesis, surface modification and applications of magnetic silica micro-spheres, which focus on the various strategies in synthesis with core-shell and mosaic structure, sol-gel method, liquid phase deposition, self-assembly technique and micro-emulsion method. Finally, foregroud and prospect of the research are also discussed.
2.Roles of angiopoietin-2, Tie-2 and hypoxia-inducible factor 1alpha in angiogenesis of glioma.
Xiao-Han LI ; Xiao-Song WANG ; Cheng-Bo HAN ; Hua-Chuan ZHENG ; Xiang-Hong YANG
Chinese Journal of Pathology 2008;37(3):188-189
Adult
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Aged
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Angiopoietin-2
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physiology
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Female
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Glioma
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blood
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physiopathology
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Humans
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Hypoxia
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blood
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metabolism
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Male
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Middle Aged
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Neovascularization, Pathologic
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physiopathology
3.Clinical Observation of Warm Acupuncture at Biguan (ST 31 ) in Treating Post-apoplectic Restless Legs Syndrome
Xiaoyu DAI ; Yan LI ; Qiuzhen SONG ; Baojie HAN ; Yuanchun XIAO
Journal of Acupuncture and Tuina Science 2006;4(3):174-175
Objective:To investigate the efficacy of warm acupuncture at Biguan(ST 31) in treating post-apoplectic restless legs syndrome. Methods:Fifty patients were randomly divided into treatment and control groups by a waiting control method in order of hospitalization. The treatment group received deep acupuncture at point Biguan(ST 31) plus moxibustion and the control group took L-dopa orally. The curative effects were compared after 28 days' treatment. Results: The total efficacy rate was 76.00% in the treatment group and 26.00% in the control group. There was a significant difference. Conclusion:Warm acupuncture at Biguan(ST 31) is effective in treating post-apoplectic restless legs syndrome.
4.Experimental study on participation of interstitial cells of Cajal and connexin 43 in the pathogenesis of diabetic gastroparesis
Han-Ni WU ; Han TIAN ; Xi-Ting ZHANG ; Wei QIAN ; Jing-Song LIU ; Xiao-Hua HOU ;
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
The changes of interstitial cells of Cajal(ICC)distribution and connexin 43(Cx43)expression in gastric muscle layers were assayed in gastroparesis models of streptozotocin-induced diabetic rats.The results showed that gastric emptying was significantly delayed,the contraction frequency and amplitude of gastric muscle segments were greatly decreased,Cx43 gray values were significantly increased and Cx43 distributed homogeneously with ICC immunopositivity in the model rats.These changes appear to be related to the pathogenesis of diabetic gastroparesis.
5.Research and advancement of RNA.
Xiao-han XIAN ; Jin-song HU ; Zhang-min YANG ; Yi-li WANG ; Lü-sheng SI
Chinese Journal of Pathology 2003;32(3):276-278
6.Chemical constituents from Pleione bulbocodioides.
Chao WANG ; Shao-Wei HAN ; Bao-Song CUI ; Xiao-Juan WANG ; Shuai LI
China Journal of Chinese Materia Medica 2014;39(3):442-447
Fourteen compoumds were isolated from the ethyl acetate portion of the 95% ethanolic extract of Pleione bulbocodioides by a combination of various chromatographic techniques including silica gel, ODS, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC, of which ten compoumds were phenanthrenes and dihydrophenanthrenes, two compoumds were bibenzyls, one was lignan and a sterol. Their structures were identified on the basis of spectroscopic data as monbarbatain A(1), 2, 7, 2'-trihy-droxy-4, 4', 7'-trimethoxy-1, 1'- biphenanthrene(2), blestriarene A(3), pleionesin B(4), shanciol H(5), 17-hydroxy-7'-(4'-hy-droxy-3 '-methoxyphenyl)- 4-methoxy-9, 10, 7', 8'-tetrahydrophenanthro[2, 3-b]furan-8'-yl methyl acetate(6), 1-p-hydroxybenzyl-4-methoxy phenanthrene-2, 7-diol(7), 1-p-hydroxybenzyl-4-met-hoxy-9, 10-dihydrophenanthrene-2, 7-diol(8), hircinol(9), coelonin( 10), gigantol(11), batatasin 11 (12), syringaresinol(13) and ergosta4, 6, 8 ( 14) , 22-tetraen-3-one (14). Compounds 1-3, 9, 13 and 14 were isolated from this genus for the first time.
Drugs, Chinese Herbal
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chemistry
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Orchidaceae
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chemistry
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Organic Chemicals
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analysis
7.Management of endometrioid carcinomapatients with preoperative diagnosis of endometrial hyperplasia
Ying SHAN ; Ying JIN ; Yan LI ; Yongxue WANG ; Jing ZENG ; Tiantian HAN ; Xiao SONG ; Lingya PAN
Basic & Clinical Medicine 2017;37(4):463-467
Objective To investigate the clinical characteristics and treatments of endometrioid carcinoma patients with preoperative diagnosis of endometrial hyperplasia.Methods From 2005 to 2010, 404 patients were diagnosed with endometrioid carcinoma after hysterectomy.Among these patients,44 of them were diagnosed atypical endometrial hyperplasia(AEH) preoperatively.Retrospectively analysis the characteristics of these patients with SPSS13.0.Results Among the 44 cases, all of them were grade G1 disease, and 39 of them received comprehensive staging surgery.14(32%)young cases preserved bilateral ovaries.9 cases(20%) were given adjuvant radiology.No recurrence was detected during the median follow up of 52 months.Compared to the premenstrual group, although no statistical difference was detected, more patients with risk factors of deep myometrium invasion(4/22 vs 1/22) and lymph-vascular space invasion(LVSI, 3/22 vs 0/22) in the postmenstrual group.Compared to the patients who diagnosed with endometrioid cancer(EC) preoperatively, there are more patients with grade G1(P=0.000), fewer patients received adjuvant chemotherapy(P=0.003) and fewer recurrence(P=0.019) in AEH group.Conclusions The endomtrioid cancer patients who diagnosed with atypical hyperplasia preoperatively have better prognosis.Hysterectomy with bilateral ovaries preserved is acceptable in young patients.Post menopause patients have more risk factors of deep myometrium invasion and LVSI.
8.Designation of gACE epitope and preparation of its polgclonal antibody
Liping AN ; Xiaoying WANG ; Xiao HAN ; Guangyu XU ; Beiying AN ; Song YANG ; Peige DU
Chinese Journal of Immunology 1985;0(03):-
Objective:To prepare gACE polyclonal antibody for functional study of gACE.Methods:According to the bioinformatics analysis and prediction of the possible conformational structure,hydrophobicity and antigenicity of gACE and the principal for antibody production,a partial peptide with 18-amino acid residues of gACE was synthesized after homology search.The synthesized peptide was then used to immunize after coupling with KLH.The properties of anti-gACE were analyzed by ELISA,Western blot and immunohistochemistry.Results:The antigenicity was repredicted by bioinformatics analysis.The polyclonal antibody against gACE was successfully obtained and its specificity and sensitivity we conformed by ELISA,Western blot and immunohistochemistry.Conclusion:By the bioinformatics analysis and prediction,the hydrophilicity and antigenicity of gACE are analyzed.The antibody of gACE is successfully obtained.
9.RNA-Seq and genuine traditional Chinese medicine.
Xiao-Yue WANG ; Jing-Yuan SONG ; Cai-Xiang XIE ; Jian-Ping HAN ; Shi-Lin CHEN
Acta Pharmaceutica Sinica 2014;49(12):1650-1657
RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at specific space and time. So it has been a research hotspot to analyze the mechanism of biosynthesis through RNA-Seq in the study on the secondary metabolism of medicinal plant. This article mainly illustrates the RNA-Seq and its advantages, it also discusses the potential application in genuine TCM, and it can provide useful information for other researchers.
Drugs, Chinese Herbal
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Gene Expression Profiling
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High-Throughput Nucleotide Sequencing
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Medicine, Chinese Traditional
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Plants, Medicinal
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genetics
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RNA
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Sequence Analysis, RNA
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Transcriptome
10.Retroperitoneal laparoscopic surgery for huge adrenal benign tumors: report of 9 cases
Shaozhong XIAN ; Bo SONG ; Xiao HAN ; Dawei CAI ; Peishan WU ; Guangqi KONG
Chinese Journal of General Practitioners 2017;16(7):554-556
The clinical data of 9 patients with huge adrenal tumors (≥6 cm) undergoing retroperitoneal laparoscopic adrenalectomy between February 2015 and September 2016 were retrospectively analyzed.There were 2 males and 7 females aged 29-65 years.The tumors were detected due to investigation of paroxysmal hypertension in 4 cases, and during the health check-up in 5 cases.The lesions were located on the right side in 4 cases, on the left side in 4 cases and bilaterally in 1 case.The operations were all successful, there was no case transferring to open surgery.The average operation time was 70 min (50-100 min),mean bleeding volum was 100 ml(50-200 ml).The postoperative diagnosis: 4 cases of pheochromocytoma, 2 cases of cortical adenoma, 2 cases of neurilemmoma and 1 case of neuroinoma.The average follow-up time was 13.5 months (6-24 months) and no recurrence was found.