Objective To investigate the alteration of serotonin-producing gastric enterochromaffin (EC)cell in patients with functional dyspepsia(FD).Methods Fifteen healthy volunteers and 33 patients with FD were enrolled.Proximal gastric mucosal EC cells were countered after immunohisto- chemistry staining.The ultrastructure of EC cell was observed by electromicroscope.Results The EC cells in proximal gastric mucosa in patients with FD were significant higher than that in controls(12.5?2.1 vs 8.3?1.4,t=2.353,P＜0.05),and the staining intensity of EC cell in patients with FD was also stronger than that in controls(3.72?0.42 vs 2.61?0.57,t=2.078,P＜0.05).The more sever the gastric mucosal inflammation was,the more number of EC cells and the stronger staining intensity were.Under the electromicroscopy,more Golgi apparatus,mitochondria and endoplasmic reticulum were found in EC cells.Special secreting particles were also found in cytoplasm.Conclusions EC cells may be involved in the pathogenesis of FD.The number of EC cell is related with the severity of gastric mucosal inflammation.

OBJECTIVETo explore a new technique for repair of alveolar cleft by sutural distraction osteogenesis.

METHODSNine 8-weeks mongrel dogs were used in this study, three being in the control group, six in the experimental group. Alveolar cleft model was created surgically in all animals. Two weeks later, a U-shaped distractor made of Ni-Ti memory alloy wire was insterted into the premaxilla to distract the mid-premaxillary suture. When the premaxilla of the cleft side approached the ipsilateral maxilla, periosteoplasty of the alveolar cleft was performed. The distractor was removed at two weeks after periosteoplasty. The results were evaluated clinically, radiographically, morphologically and histologically.

RESULTSThe cleft model in dogs was stable and similar to the human alveolar cleft. In experimental dogs, the premaxilla was moved gradually toward the maxilla so that the cleft was closed. The distracted mid-premaxillary suture showed a gradually widened traingle, with its tip being posterior. The density of the distracted traingle suture was increased gradually. Bony repair was achieved completely at the cleft three months post-periosteoplasy. The morphology of the mid-premaxillary suture was restored.

CONCLUSIONThe alveolar cleft could be repaired by the technique of mid-premaxilla suture distraction.

Alveoloplasty ; methods ; Animals ; Dogs ; Maxilla ; surgery ; Models, Animal ; Osteogenesis, Distraction ; methods

Objective To explore the clinical pathway of operable and evaluable psychological nursing for elderly patients so as to improve the psychological nursing competence of duty nurses.Methods Sixty elderly patients were randomly divided into control group( n =30 cases) which received traditional psychological nursing and pathway group( n =30 cases) which received psychological nursing in clinical pathway.Then,patients' satisfaction to psychological nursing service and the effect of psychological nursing pathway on competence of duty nurses were compared.Results The rate of emotional attention in nurses,psychological nursing content,mastery of relax skill,mental support feeling and patients' satisfaction in pathway group was 96.67％,86.67％,93.33％,100％,respectively,which were higher than 63.33％,36.67％,6％,36.67％,80％in control group,and difference was statistically significant (x2 =10.42,15.86,45.06,27.80,4.04,respectively;P ＜0.05 or P ＜0.01 ).The score of health education,assessment and intervention,communication and cooperation of nurses after clinical pathway application of psychological nursing respectively was (94.00 ±0.42),(87.61 ±7.09),(98.40 ±0.69) significantly higher than (92.80 ±0.49),(61.3.0 ±9.50),(93.70 ±1.24) before application (t =11.76,14.04,20.95,respectively; P ＜ 0.05 ).Conclusions Application of psychological nursing pathway is good for improving competence of nurses and promoting psychological nursing service in geriatric internal medicine wards.

OBJECTIVETo probe the possibility of a new technique of primary cleft palate repair by sutural distraction osteogenesis.

METHODSThe distractor was made of TiNi-shape memory alloy with 2 secure and 2 active arms. Under general anesthesia, 1 hole was drilled on each maxillary process and horizontal plate of palatine. The four arms of the distractor were inserted into holes. The palatine bones would then be distracted and moved medially and posteriorly. After 4 to 5 months' distraction, the two cleft edges approached gradually. The residual cleft, according to its width, was repaired by direct stitch of muco-periosteum incised at cleft edges, or with an additional relaxing incision on one side of the palate. Direct measurement of width of maxillary arch and the cleft, and the length of the hard palate was performed before and after distraction to evaluate the effects of distraction.

RESULTSEight cleft children at their 2 to 4 years of age were treated by the new technique. Two of them terminated the procedure due to premature dislocation of the distractor. Six children underwent 37 to 126 days' distraction. Dramatic narrowing of the cleft and lengthening of hard palate were seen in these children, with the average narrowing of the cleft being 6.5 mm and average lengthening of the hard palate being 4.8 mm.

CONCLUSIONIt is clinically demonstrated that palatal tissue regeneration and cleft closure or narrowing and hard palate lengthening could be achieved by the technique of sutural distraction osteogenesis.

Child, Preschool ; Cleft Palate ; surgery ; Humans ; Maxilla ; Osteogenesis, Distraction ; instrumentation ; methods ; Palate, Hard ; surgery ; Suture Techniques ; Time Factors

OBJECTIVETo probe the feasibility of a new technique of sutural distraction osteogenesis for midface hypoplasia repair.

METHODSA protraction system was developed, which consisted of three parts: a rigid external framework, a bone hook, and the elastic band. Four 12-week mongreal dogs were randomly assigned into two groups: the control (n = 1) and the experimental groups (n = 3). Four pairs of titanium bone markers were fixed on either side of the bone sutures of all animals. The experimental group was fitted with a distraction device, through which a forward elastic force was exerted for 1 month. Clinical observations and X-ray examinations were performed at the beginning and the end of distraction. New bone specimens were examined histologically.

RESULTSAll the animals in the experimental group showed progressively forward movement of the maxilla and manifested class II occlusion at the end of the experiment. The distance increase between each pair of bone markers showed that premaxilla advancement was more than that of the maxilla. The next was zygoma. There was no obvious difference between the control and the experimental group in terms of the basilar-maxillary angle and counterclockwise rotation. Theer was active osteogenesis in the sutures, especially in premaxilla-maxillary sutures. The number of osteoblasts and fibroblasts increased dramatically and new bone formation was found at the edge of the suture.

CONCLUSIONThis technique can create effective advancement of the maxilla. The direct force can avoid counterclockwise rotation of the maxilla during distraction.

Animals ; Dogs ; Maxilla ; abnormalities ; diagnostic imaging ; surgery ; Osteogenesis, Distraction ; methods ; Radiography ; Random Allocation ; Suture Techniques

Objective To investigate the efficacy and safety of early rehabilitation programme in elderly patients after percutaneous coronary intervention (PCI).Methods A total of 96 elderly patients treated by PCI were randomly divided into rehabilitation group and control group.In rehabilitation group,the patients received the early cardiac rehabilitation programs,while the control group received the traditional rehabilitation therapy.The echocardiography and complications of two groups were observed and compared.Results During hospitalization,the left ventricular end diastolic diameter,left ventricular end systolic diameter,left ventricular posterior wall thickness and left ventricular ejection fraction in the two groups were no significant difference (P ＞ 0.05).While the complications incidence of rehabilitation group such as irritability (8.33％ vs 27.08％),insomnia (14.58％ vs 39.58％),abdominal distension (6.25％ vs 33.33％),low back pain(4.17％ vs 29.17％),point-pricking bleeding (2.08％ vs 16.67％) were significantly lower than that in the control group (x2 ＝ 5.790,7.596,11.090,10.800,6.008,respectively;P ＜0.05).Conclusions Early postoperative cardiac rehabilitation program in elderly patients with PCI is safe and helpful,which can significantly reduce the incidence of complications.

Chronic Disease ; Coronary Occlusion/surgery* ; Humans ; Treatment Outcome ; Ultrasonography, Interventional

Treatment of refractory idiopathic intracranial hypertension (IIH) is a challenging problem. We reported a refractory IIH patient who manifested with typical intracranial hypertensive symptoms successfully treated with endovascular stent implantation. Pre-operative cerebrospinal fluid (CSF) opening pressure is 36 cmH2O. Cerebral angiography demonstrated a stenotic lesion located at the right transverse sinus (TS). The stenotic TS returned to its normal caliber and the pressure gradient deceased from 36 mmHg to 4 mmHg after the stent placement. The intracranial hypertensive symptoms resolved and one month later, the CSF opening pressure decreased to 14 cmH2O.
Cerebral Angiography ; Female ; Humans ; Middle Aged ; Pseudotumor Cerebri ; diagnostic imaging ; surgery ; Transverse Sinuses ; diagnostic imaging ; surgery

OBJECTIVETo investigate the distribution characteristics of serum specific IgE (sIgE) for inhaled allergens in children with different airway allergic diseases.

METHODSFluorescent enzyme-linked immunosorbent assay on the UniCAP250 system was performed to measure serum sIgE for 9 common inhaled allergens in 256 children aged 3-14 years with different airway allergic diseases. According to the clinical diagnosis, these children were divided into rhinitis group (37 children with allergic rhinitis), asthma group (82 children with bronchial asthma), and rhinitis-asthma group (137 children with allergic rhinitis complicated by bronchial asthma). The three groups were compared in terms of the detection rates of 9 inhaled allergens, sensitization level, and number of allergens.

RESULTSThe detection rate of serum sIgE for inhaled allergens was 57.3% (47/82) in the asthma group, 86.5% (32/37) in the rhinitis group, and 82.5% (113/137) in the rhinitis-asthma group (P<0.05). The most common allergen in the asthma, rhinitis, and the rhinitis-asthma groups was mould fungi (32.9%, 54.1%, and 48.9% respectively), followed by dust mites (30.5%, 45.9%, and 46.0% respectively), pollen (26.8%, 35.1%, and 32.8% respectively), pets (12.2%, 27.0%, and 18.2% respectively), and cockroach (9.8%, 5.4%, and 5.8% respectively). The rhinitis group and the rhinitis-asthma group had a significantly higher detection rate of mould fungi (mx2) than the asthma group (P<0.0166). There were no significant differences in the sensitization level of 9 allergens and number of allergens between the three groups.

CONCLUSIONSIn children with either bronchial asthma, allergic rhinitis, or bronchial asthma complicated by allergic rhinitis, the three most common inhaled allergens are mould fungi, dust mites, and pollens. Compared with bronchial asthma, allergic rhinitis may be more closely associated with sensitization by mould fungi. The three common airway allergic diseases have similar distribution characteristics of inhaled allergens.

Adolescent ; Allergens ; immunology ; Asthma ; immunology ; Child ; Child, Preschool ; Female ; Humans ; Immunoglobulin E ; blood ; Male ; Rhinitis, Allergic ; immunology

OBJECTIVE: To determine the association between asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide (NO) synthase, with atherosclerosis in patients with chronic kidney disease (CKD). METHODS: One hundred thirty-eight CKD patients were enrolled in this study. Serum levels of L-arginine, ADMA, and SDMA were measured by high-performance liquid chromatography (HPLC). Common carotid arteries intimae-medial thickness (CCA-IMT), cross-sectional calculated intimae-medial thickness (cIM area) and atherosclerotic plaque were detected by noninvasive high-resolution B-mode ultrasonography. RESULTS: Serum levels of ADMA and SDMA were significantly increased in CKD patients (n=138) compared with age matched healthy subjects (n=42, P<0.01). ADMA and SDMA levels increased with the progression of renal dysfunction and were negatively related to creatinine clearance (Ccr) in pre-dialysis patients (r=-0.315, P<0.05; r=-0.426, P<0.01). Serum levels of ADMA and SDMA in dialysis patients (n=74) were significantly higher than those in pre-dialysis patients (P<0.05). Patients with carotid artery plaques showed significantly higher levels of ADMA compared with those without plaques. Serum levels of ADMA closely correlated with the mean IMT (r=0.471, P<0.01) and cIM area value (r=0.430, P<0.01). These correlations remained significant even after adjusting GFR, age, gender ,and other risk factors for atherosclerosis in the multiple regression analysis. CONCLUSION Serum levels of ADMA increased with the progression of CKD and may play a role in the pathogenesis of accelerated atherosclerosis in CKD patients.
Adult ; Arginine ; analogs & derivatives ; blood ; Carotid Artery Diseases ; blood ; etiology ; Female ; Humans ; Kidney Failure, Chronic ; blood ; complications ; therapy ; Male ; Middle Aged ; Nitric Oxide Synthase ; antagonists & inhibitors ; Renal Dialysis