ObjectiveTo investigate the feasibility and importance of the application of simulation ward in medical practice between classes.MethodsTo divide the interns into two groups:the experimental group of 30 and the control group of 30. Both groups apply the simulation ward to practice between classes and both are assessed at the end of the practice.ResultsTo compare the final score of the experimental group and the control group,and significant differences are found in the following aspects:humanistic care,history taking,physical examination,medical record analysis,the theoretical knowledge examinations with the data of P ＜0.05.ConclusionThe application of simulation ward in medical practice between classes can improve the quality of clinical teaching.

Objective To investigate the correlation of uric acid(UA) level and the circadian rhythm of blood pressure in hypertensive patients. Methods Among the individuals who presented to the cardiology clinic, 70 patients who had hypertension and were diagnozed with non- dipper hypertension (non-dipper hypertension group) by 24 h ambulatory blood pressure monitoring (ABPM), 70 patients with dipper hypertension patients (dipper hypertension group), and 52 normotensive individuals (control group) were enrolled in this study. Peripheral venous blood samples were collected from all the patients in order to evaluate the hematological and biochemical parameters. All the assessed parameters were compared among three groups. Results The level of UA in non-dipper hypertension group was the highest, in dipper hypertension group was higher and in contrl group was the lowerst:(393.57 ± 53.52), (280.57 ± 41.64), (267.66 ± 59.38) μmol/L, and there were significant differences (P<0.01). Multivariate Logistic regression analysis revealed that the level of UA was an independent risk factor for non-dipper circadian rhythm of blood pressure (P = 0.003, OR = 2.26, 95% CI: 1.34- 3.89). Conclusions The higher level of UA may be a risk factor for non-dipper circadian rhythm of blood pressure in hypertension patients.

Objective To discuss the nursing for patients with articular cartilage injury of knee joint after arthroscopic and stem cells transplantation.Methods With autologous bone marrow stem cell as the parent,stem cells were transplanted to patients' knee joints after separation and differentiation in vitro,promoting the recovery and reconstruction of the cartilage.Nursing intervention was implemented to 16 patients from preoperative preparation until after the surgery,to enhance patients' sense of self-protection and encourage them to do functional exercise actively.Results The success rate of operation was 100％ for the 16 patients,whose function was improved obviously after 4 weeks without recurrence and exacerbation.Conclusions Arthroscopic and stem cells transplantation can effectively alleviate the symptoms of articular cartilage injury of knee joint,and the whole nursing intervention can promote patients' functional recovery as soon as possible.

Background The excitotoxicity to retinal neurons caused by abnormal elevation of glutamate in retina is a common pathology concomitant with major blind-causing eye diseases.However,an effective approach to protect retinal neurons from glutamate-induced excitotoxicity is still lack.Intraperitoneal administration of α-melanocyte stimulating hormone(α-MSH)has been shown to protect hippocampal neurons from glutamate-induced excitotoxicity.Objective This study was to investigate the protective effect of α-MSH on glutamate-induced excitotoxicity in a chicken embryonic retinal explant culture system.Methods The retinas were isolated from chick embryos at embryonic day 9(E9) and cultured as explants.The explants at 3,5 and 7 days in vitro and the retinas at corresponding embryonic day 12,14 and 16(E12,E14,E16)were collected.The morphology of explant cultures was examined by hematoxylin and eosin staining,and the expression of melanocortin receptors (MCRs)was analyzed by real-time PCR.In the experiment of glutamate-induced excitotoxicity,the retinal explants at 4 days in vitro were treated with glutamate for 48 hours,α-MSH was incubated with the explants 30 minutes before and during the glutamate treatment period.Then the apoptotic cells were detected by TUNEL staining and quantified.The glutamate alone treated-explants and those treated with culture media were included as controls.The expression of glial fibrillary acidic protein(GFAP) at 48 hours after treatment in all retinal explants was analyzed by real-time PCR.Results Hematoxylin and eosin staining showed that the retinal explants exhibited similar morphology to those observed in the retinas from chick embryos at the corresponding developmental stages.The real-time PCR analyses of chick retinas showed that MC1R mRNA level at E9,E12,E14 and E16 was significantly lower than that in post-hatch day 1 (all P=0.000) ;whereas the transcript level of MC5R was significantly increased from E9 to E12 and E14 (both P =0.000),and then gradually decreased from E14 to P1.The expression of these genes showed similar temporal patterns in the retinal explant cultures.TUNEL staining revealed that treatment of the retinal explant cultures with α-MSH substantially and significantly reduced number of apoptotic cells induced by glutamate (P =0.000),which was accompanied by significant suppression of glutamate-induced GFAP up-regulation (P =0.000).Conclusions Application of α-MSH dramatically ameliorated glutamate-induced cell death in retinal explant cultures.This protective effect may be due to α-MSH-mediated suppression of astrogliosis caused by abnormal elevation of glutamate.

In the past few decades, our understanding of platelets has made great progress. Platelets play an unexpected central role in cancer and greatly affect the behavior of cancer cells. At the same time, the physiology and phenotype of platelets are also affected by cancer cells. Therefore, platelet-based tumor targeted therapy strategies have attracted the attention of researchers, but the limitations of their application require more attention. In this paper, the strategies of platelet-based tumor targeted therapy are summarized, and the strategies of platelet mimicking nanocarrier delivery, platelet hitch riding, platelet membrane coating biomimetic and engineered platelet targeting are mainly introduced. The easy activation, hard storage and unknown functional and phenotypic changes of platelets were discussed. At the same time, the strategy of platelet-based targeted tumor therapy is reviewed from theoretical basis and practical application. The development potential of platelets in the field of tumor diagnosis and treatment is discussed, which will provide some theoretical reference for the study of platelet-related tumor diagnosis and targeted therapy.

Objective To investigate the effect of sperm -associated antigen 9 ( SPAG9 ) on migration and invasion abilities of human osteo-sarcoma U2OS cells.Methods siRNA was used to decrease SPAG9 expression, and then cells were subjected to CCK -8 assay, wound healing assay and transwell assay to detect siSPAG9 on cell migration and invasion abilities. Western blot and Gelatin zymography were used to detect matrix metalloproteinase-2 ( MMP-2) and matrix metalloprotei-nase-9 ( MMP -9 ) expression and activities. Results siRNA for SPAG9 significantly decreased SPAG9 mRNA and protein expression. siSPAG9 had no effect on the proliferation of U2OS cells, but significant-ly inhibited its wound healing, migration and invasion abilities. The activities and expression of MMP-2 amd MMP-9 were also suppressed by siRNA for SPAG9.Conclusion Depletion of SPAG9 results in the inhi-bition of migration and invasion abilities of human osteosarcoma U2OS cells.

OBJECTIVETo test the reliability and validity of Meaningful Life Measure-Chinese Revised (MLM-CR) in Chinese college students.

METHODSA total of 1035 college students were evaluated with MLM-CR, Satisfaction with Life Scale (SWLS), Purpose in Life (PIL) and Patient Health Questionnaire-2 (PHQ-2), and 120 of the students were examined with PIL-SF twice.

RESULTSAll the items in MLM-CR had good discrimination indexes (r=0.753-0.838, P<0.001). Confirmatory factor analysis confirmed the hypothesized five-factor model of MLM-CR (Χ/df=3.4, GFI=0.946, AGFI=0.924, RMR=0.069, NFI=0.953, CFI=0.966, RMSEA=0.048). The total internal consistency reliability of MLM-CR was 0.942, and the alpha coefficients of the 5 dimensions ranged from 0.782 to 0.877; the total split-half reliability was 0.920, and the split-half reliability of the 5 dimensions ranged from 0.752 to 0.830; the total test-retest reliability was 0.871, and the test-retest reliability of the 5 dimensions ranged from 0.783 to 0.805. The criterion validity of MLM-CR in correlation with SWLS, PIL and PHQ-2 was 0.66, 0.755 and -0.388, respectively (P<0.01). The Average score of MLM-CR of the college students was 5.20∓0.90, and the scores were significantly higher in female students than in the male students (P<0.001).

CONCLUSIONMLM-CR has good psychometric properties for application in comprehensive evaluation of personal meaning in life.

Asian Continental Ancestry Group ; China ; Factor Analysis, Statistical ; Female ; Humans ; Male ; Psychometrics ; Reproducibility of Results ; Students ; Surveys and Questionnaires

OBJECTIVETo compare the results of application of Qu single abdominal aorta clamping for bloodless hepatectomy and Pringle hepatectomy in 118 cases of liver tumors.

METHODSThe clinical data of 118 patients, including 59 patients undergoing Qu single abdominal aorta clamping for bloodless hepatectomy (Group QG) and 59 patients undergone Pringle first hepatic portal clamping hepatectomy (Group PG) since March 2009 in the Ningbo Tumor Hospital and Jiangxi Provincial Hospital were retrospectively reviewed. The changes of blood pressure, oxygen saturation, urine volume, intravenous fluid volume, amount of bleeding, time of abdominal aorta (or first hepatic portal) clamping, duration of operation and anesthesia, and other intraoperative indexes of the two groups were compared, and the changes of peritoneal drainage, blood tests, liver functions, etc. before operation and 1, 3, 7, 14 days after the hepatectomy in the two groups were also analyzed.

RESULTSAfter taking appropriate measures for intraoperative blood pressure control, only small fluctuations of blood pressure, which could be safely adjusted and controlled with stable vital signs, was observed in the group QG. The amount of intraoperative bleeding in the group QG was (96.25 ± 18.45) ml, significantly less than (536.25 ± 35.65) ml in the group PG (P < 0.05). In the group QG, both the duration of operation time [(227.58 ± 28.20) min] and duration of anesthesia [(249.48 ± 31.35) min] were significantly shorter than that [(261.46 ± 32.12) min and (286.58 ± 35.62) min, respectively] in the group PG (both P < 0.05). The postoperative liver dysfunction in the group QG was also milder than that in the group PG (P < 0.05).

CONCLUSIONSFor liver tumor patients, Qu single abdominal aorta clamping for bloodless hepatectomy can basically achieve the goal of bloodless hepatectomy. This surgical operation is simple and safe, worthy of recommendation to skillful liver surgeons in hospitals there are some difficulties of blood supply.

Adolescent ; Adult ; Alanine Transaminase ; blood ; Aorta, Abdominal ; Aspartate Aminotransferases ; blood ; Blood Loss, Surgical ; Blood Pressure ; Carcinoma, Hepatocellular ; blood ; surgery ; Constriction ; Female ; Hemangioma, Cavernous ; blood ; surgery ; Hepatectomy ; methods ; Humans ; Liver Neoplasms ; blood ; surgery ; Male ; Middle Aged ; Operative Time ; Portal Vein ; Retrospective Studies ; Serum Albumin ; metabolism ; Young Adult

OBJECTIVETo investigate the expression and clinical implication of tissue inhibitor of metalloproteinase-1 (TIMP-1) in colorectal carcinoma.

METHODSTIMP-1 expression in 54 colorectal carcinoma was observed by SP immunohistochemical method, and the results were analyzed in relation to the clinical data of patients.

RESULTSTIMP-1 was localized on the membrane and in the cytoplasm of the enteric epithelial cells, and its expression rate was 100% in normal tissue but only 59.6% (31/52) in colorectal carcinoma tissues. In addition, the expression rate of TIMP-1 was higher in the tumor tissues without lymph node metastasis than in tissues with lymph node metastasis (P<0.05).

CONCLUSIONThe expression of TIMP-1 is inversely correlated to lymph node metastasis of colorectal carcinoma, and decreased TIMP-1 expression may play a role in the progression of colorectal carcinoma.

Adult ; Aged ; Colorectal Neoplasms ; metabolism ; pathology ; Epithelial Cells ; chemistry ; pathology ; Female ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Middle Aged ; Tissue Inhibitor of Metalloproteinase-1 ; biosynthesis

Objective To explore the relationships among negative life events,meaning in life and suicidal risk of college students.Methods A total of 897 students from southern medical university were surveyed by adolescent life events scale (ALES),suicidal behaviors questionnaire-revised (SBQ-R) and meaningful life measure-Chinese revised (MLM-CR).Results The scores of negative life events,meaning in life and suicidal risk were 1.03±0.88,124.32±20.35 and 4.17± 1.66,respectively.There were 64 (7.1％) college students with high risk of suicide.The overall stress score was positively correlated with total suicidal risk score (r=0.134,P＜0.01).Total suicidal risk score was negatively correlated with total score of meaning in life(r=-0.340,P＜0.01).Moderating effects of meaning in life between negative life events and suicidal risk were significant (P＜0.01).Meaning in life fully mediated the effects of negative life events on suicidal risk (P＜0.01),which accounted for 59.6％ of the total effects.Conclusion Meaning in life as a mediator and moderator between negative life events and suicidal risk.Meaning in life might play protective role in reducing the suicidal risk.