Objective To research and develop a computer-based patient record (CPR) system so as to realize the collection, processing, storage, transmission and application of patient information. Methods A CPR system was accomplished through developing a structural patient record, a text editor, techniques of database security, a knowledge base of on-line help, real-time monitoring, print control and function expansion. Results The CPR system, established with the above techniques, was put into use in two third-tier hospitals. It was proved via practice that the system, sound in operation, safe and stable, easy to maintain, and compatible, enhanced medical quality and clinical efficiency. Conclusion ①Creating a structural patient record is the basis of realizing CPR. ②Developing a specialized editor is the key to bringing about CPR. ③Possessing perfect database security techniques is the guarantee for starting CPR. ④Constructing a knowledge base of on-line help is an effective way to help doctors raise the level of their clinical decisions. ⑤The CPR system is an effective means of improving the quality of patient records.⑥The CPR system is also an effective means of improving the efficiency of patient record writing.

Objective To establish a new method for rapid detection of electrolytes based on micro spectrometer and dry reagent.Methods The liquid electrolyte reagent was lyophilized for the preparation of reagentin powder form,which was then sealed into a detector cup of micro spectrometer for later experiment.During determination,the detector cup,in which the specimens and diluents were added to the dry reagent,was put into the detector slot of micro spectrometer,the contents in the detector cup were then well mixed by the magnetic stirring system of micro spectrometer and incubated for 6min with the heating system.Afterwards,the A values of potassium ion,sodium ion and chloride ion were detected respectively at 620nm,405nm and 456nm following the reaction principles of turbidimetry,enzymatic method and chemical method.Based on the findings of those detections mentioned above,the performance of the electrolytic dry reagent was evaluated,and the results were then statistically analyzed.Results The linear range of each parameter could meet the demand for clinical analysis,and the dry reagents had good reaction stability for 90 days after being lyophilized,with the intra-assay coefficient variation(CV) less than 4%,inter-assay CV less than 5%,and the recovery rate from 95% to 105%.No obvious interference was observed in the determination results under the circumstance that the serum TB was less than 290.40?mol/L and the TG was less than 11.20mmol/L.The detection results by this method were well correlated with that of dry chemical analysator VITRO S-250(R≥0.98).Conclusion The method is accurate and reliable in determining the serum electrolytes,and its process is simple process and convenient to carry out.Therefore,it can satisfy the condition for field aid as well as primary care.

Objective To compare the ocular parameters measured by 2 biometry devices Tomey OA-2000 and IOLMaster in cataract eyes.Methods A total of 74 cataract patients (74 eyes) were included in this prospective study.Ocular parameters,including axial length (AL),keratometry (K),anterior chamber depth (ACD) and white to white (WTW),were obtained from Tomey OA-2000 and IOLMaster,respectively.Paired t-test was applied to analyze the differences of ocular parameters from Tomey OA-2000 and IOLMaster,while Bland-Altman plot was used to assess the agreement of these ocular parameters.Results The AL,flat K,steep K,mean K,central corneal thickness (CCT),ACD,lens thickness,pupil diameter and WTW values measured by Tomey OA-2000 were (24.26 ± 2.71) mm,(44.09 ± 1.76) D,(45.01 ± 1.73) D,(44.55 ± 1.72)D,(518.41 ±34.18) μm,(3.07 ±0.48)mm,(4.48 ±0.61)mm,(4.07 ±0.95)mm and (11.50 ± 0.52)mm respectively.Values of AL,flat K,steep K,mean K,ACD and WTW from IOLMaster were (24.33 ± 2.69) mm,(44.21 ± 1.74) mm,(45.15 ± 1.76) D,(44.69 ± 1.74) D,(3.04 ± 0.46)mm and (11.65 ± 0.41) mm respectively.The difference values of AL,flat K,steep K,mean K,ACD and WTW between Tomey OA-2000 and IOLMaster were (-0.07 ±0.10) mm (t =-5.99,P＜0.01),(-0.12 ± 0.21) D (t =-4.14,P＜0.01),(-0.14 ± 0.20)D (t =-5.88,P＜0.01),(-0.12 ±0.13) D (t =-7.44,P＜0.01),(0.03 ±0.14) mm (t=1.99,P=0.05) and (-0.14±0.32) mm (t=-3.73,P＜0.01) respectively.The 95％ limits of agreement (LoA) of the AL,flat K,steep K,mean K,ACD obtained from the two devices was 0.27 mm,0.50 D,0.53 D,0.38 D,0.30 mm,respectively,and the agreement was good;95％ LoA of WTW was 0.78 mm,and the consistency was poor.Conclusion The differences of AL,K values and ACD between Tomey OA-2000 and IOLMaster were small,suggesting the agreement is unified.

OBJECTIVETo study whether the analgesis of oxymatrine (OMT) affects N-type voltage-gated calcium channels (VGCCs).

METHODSTotally 45 mice were randomly divided into the sham-operation group, the model group [established by partial sciatic nerve ligation (PSNL)] , and the OMT treatment group according to random digit table, 15 in each group. The dorsal root ganglions (DRG) were separated in PSNL pain model mice. Intracellular calcium concentration ([Ca2+]i) was determined with Fluo-3 AM immunofluorescent probe in cultured DRG neurons. Different protein expression levels of N-type (Cav2. 2) and L-type ( Cav1. 3) among VGCCs from brain and DRG tissues were detected with Western blot.

RESULTSCompared with the sham-operation group, [Ca2+]i, increased in cultured DRG neurons (P <0. 05) , protein expression levels of Cav2. 2 in the brain tissue increased (P <0. 05), protein expression levels of Cav2. 2 in DRG tissues decreased in the model group (P <0. 01). Compared with the model group, [Ca2+]i, decreased in cultured DRG neurons (P < 0. 05), protein expression levels of Cav2. 2 in the brain tissue decreased (P <0. 01), protein expression levels of Cav2. 2 in DRG tissues increased in the OMT treatment group (P <0. 01). There was no statistical difference in Cav1. 3 expressions in cultured DRG neurons and the brain (P >0. 05).

CONCLUSIONAnalgesic effect of OMT might be related to Cav2. 2 channel mediated calcium ion flux.

Alkaloids ; pharmacology ; Analgesia ; methods ; Analgesics ; pharmacology ; Aniline Compounds ; Animals ; Calcium ; Calcium Channels, N-Type ; physiology ; Ganglia, Spinal ; Mice ; Neurons ; Pain ; Quinolizines ; pharmacology ; Xanthenes

Objective: To observe the effects of interferon-induced protein 204 (p204) over-expression on apoptosis, proliferation and migration of aortic vascular adventitial ifbroblast (VAFs) in experimental rats.
Methods: Our research included in 3 groups: Iif204-Lv group, in whichVAFs were infected by Iif204-recombined lentivirus, Con-Lv group, in which VAFs carried the empty vector without virus, Blank control group, in which VAFs were untreated. VAFs proliferation was examined by MTT method, cell apoptosis was measured by lfow cytometry and the migration was detected by scratching assay and transwell chamber method. The mRNA and protein expressions of p204, p53 and p21were evaluated by real-time q RT-PCR and Western blot analysis respectively.
Results: Compared with Con-Lv and Blank control groups, Iif204-Lv group had decreased VAFs proliferation (by OD value) at 48 hours: (0.53 ± 0.05) vs (0.66±0.03) and (0.63 ± 0.06), at 72 hours: (0.89 ± 0.06) vs (1.02 ± 0.06) and (1.01 ± 0.07); distance of cell migration (by pixel): (61.00 ± 1.83) vs (74.50 ± 6.25) and (75.50 ± 7.85); number of cell migration: (61.75 ± 10.69) vs (155.25 ± 10.21) and (153.75 ± 9.40), allP<0.05. VAFs apoptosis rates were similar among different groups. Compared with Con-Lv and Blank control groups, Ifi204-Lv group presented up-regulated mRNA expressions of p204 (3.45 ± 0.15) vs (2.09 ± 0.10) and (2.06 ± 0.09); p53 (3.41 ± 0.09) vs (2.06 ± 0.07) and (2.10 ± 0.06); p21 (3.01 ± 0.08) vs (2.05 ± 0.06) and (2.11 ± 0.08), allP<0.05.
Conclusion: p204 over-expression inhibits VAFs proliferation and migration which might be partly related to the activation of p53 and p21 expression in experimental rats.

Objective: To study the impact of interferon-inducible protein 16 (IFI16) inhibition on apoptosis of human aortic adventitial fibroblasts (HAAFs). Methods: Our research included 3 groups: ① IFI16-siRNA group, specific small interference RNAs (siRNAs) of IFI16 were transfected into HAAFs in vitro to make IFI16 gene silence, ②Con-siRNA group, non-specific siRNAs were transfected into HAAFs as negative control and ③Untreated HAAFs group, blank control. HAAFs cell cycle and apoptosis rate were examined by flow cytometry, IFI16 mRNA expression was measured by real time qRT-PCR, protein expressions of IFI16, p53, p21, Bax and Bcl-2 were detected by Western blot analysis. Results: Compared with Con-siRNA group and Untreated HAAFs group, IFI16-siRNA group showed decreased apoptosis rate of HAAFs (3.33±0.41) % vs (7.42±1.51) % and (6.49±1.10) %, P<0.05, reduced ratio of G0/G1 phase cells (56.64 ± 4.77 ) % vs (69.67±3.54) % and (68.29±4.14) %, P<0.05, while increased ratio of S phase cells (25.23±5.19)% vs (13.76±2.07) % and (14.04±3.00) %, P<0.05. Meanwhile, IFI16-siRNA group presented down-regulated IFI16 mRNA and protein expressions, decreased protein levels of p53, p21, Bax and increased protein level of Bcl-2, all P<0.05. Conclusion: Inhibited IFI16 expression could decrease HAAFs apoptosis, promote cell cycle transition from G1 to S phase which might be related to the suppression of p53/p21 signaling pathway and regulation of Bax/Bcl-2 expression.

Background Spectral-domain optical coherence tomography (SD-OCT) can quantitatively analyze some ocular parameters in vivo.Although human ocular parameters have been obtained by SD-OCT,few studies were performed in animal experiment.Objective This study was to investigate the anterior and posterior segment parameters of C57BL/6 mice and pigmented rabbits using SD-OCT in vivo.Methods Some anterior and posterior segment ocular parameters,including the central corneal thickness (CCT),anterior chamber depth (ACD),white-to-white (WTW),optic nerve head (ONH) depth/width and retinal thickness,were measured in 8 eyes of 4 health SPF C57BL/6 mice and 12 eyes of 6 health SPF pigmented rabbits using SD-OCT.Results For C57BL/6 mice,Cornea,iris,lens in pupil area were clearly exhibited by SD-OCT.Mean CCT,ACD and WTW were (96±9)μm,(460±8) μm and (2.86 ± 0.41) mm pre-mydriasis,respectively,the corresponding values of post-mydriasis were (96±8) μm,(356±20)μm and (2.87±0.62)mm.There were no statistical differences of CCT and WTW between pre-and post-mydriasis (t =0.478,P =0.647 ; t =-0.737,P =0.485).ACD of post-mydriasis was significantly shallower than that of baseline (t =-13.022,P＜0.001).For the pigmented rabbits,the thickness of corneal thinnest point,retinal thickness,ONH depth and width were (370 ± 10) μm,(175 ± 4) μm,(1.35 ± 0.51) mm and (4.52±0.82) mm,respectively.Conclusions As a non-contact and non-invasive technology,SD-OCT can provide not only high resolution cross-sectional ocular images,but also high precise quantitative parameters for both C57BL/6 mouse and pigmented rabbit in vivo.

Aim To evaluate the effects of pantoprazole on various experimental acute ulcer inrats and mice. Methods The model of a gastric ulcer of rats or mice was caused bystree- induced ulcer and ligatel pylurus-induced ulcer. Results & Conclusions At adose of 5, 10, 20 mg? kg-1 of Pantoprazole can markedly decrease the ulcer index ofstree-induced ulcer. Pantoprazole(4, 8, 16 mg? kg -1 ) significantly decrease the areaof ligated pylorus-induced gastric ulcer. It was also found that pantoprazole caninhibit the output of basic gastric acid.

Adult ; Atropine ; therapeutic use ; Bronchodilator Agents ; therapeutic use ; Combined Modality Therapy ; Female ; Hemofiltration ; Humans ; Insecticides ; poisoning ; Male ; Middle Aged ; Organophosphorus Compounds ; Poisoning ; therapy ; Treatment Outcome

Adult ; Aged ; Aged, 80 and over ; Carcinoma in Situ ; virology ; Carcinoma, Squamous Cell ; virology ; Female ; Human papillomavirus 16 ; isolation & purification ; Human papillomavirus 18 ; isolation & purification ; Humans ; Microarray Analysis ; methods ; Middle Aged ; Papillomaviridae ; isolation & purification ; Papillomavirus Infections ; diagnosis ; Paraffin Embedding ; Polymerase Chain Reaction ; methods ; Uterine Cervical Neoplasms ; virology