1.Treatment of Recurrent Oral Ulceration (Yin Deficiency Fire Excess Type) by Qianjin Kouchuang Jiawei Granule: a Clinical Observation.
Yan HAN ; Pei-yuan LIU ; Jing XIAO
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(7):816-819
OBJECTIVETo observe the curative effect of Qianjin Kouchuang Jiawei Granule (QKJG) on recurrent oral ulceration [yin deficiency fire excess type (YDFET)].
METHODSTotally 120 patients who suffered from recurrent oral ulceration (YDFET) were randomly assigned to two groups, the experiment group and the control group, 60 in each group. Patients in the experiment group took QKJG, 20 g each time, twice per day, while those in the control group took Kouyanqing Granule (KG) , 20 g each time, twice per day. Fourteen days consisted of one therapeutic course, two for all. Scores for patients' symptoms and signs (ulcer area, exudation, hyperaemia, edema, the number of ulceration, burning sensation, and pain degrees) were assessed before treatment, at day 3 and 7 after treatment. Short-term efficacy was assessed by visual analogue scale (VAS). The total paralysis time and the total number of ulceration at month 12 after treatment were taken as judgment for long-term efficacy. Results Compared with before treatment in the same group, symptoms and signs were obviously improved at day 3 and 7 after treatment in the two groups (P < 0.05). Compared with the control group at day 3 after treatment, the improvement of edema, exudation, pain degree, and burning sensation was more obvious in the experiment group (P < 0.05, P < 0.01). The improvement of edema, pain degree, and burning sensation at day 7 after treatment was more obvious in the experiment group than in the control group (P < 0.05). As for short-term efficacy, the total effective rate was 86.67% (52/60 cases) in the experiment group and 83.33% (50/60 cases) in the control group, with no statistical difference between the two groups (P > 0.05). As for long-term efficacy, the total effective rate was 90.00% (54/60 cases) in the experiment group, significantly higher than that of the control group with statistical difference [81.67% (49/60 cases), P < 0.05]. At month 12 after treatment, the total number of ulceration was reduced and the paralysis time of ulcer attack prolonged in the experiment group, with statistical difference when compared with the control group (P < 0.05).
CONCLUSIONQKJG showed better long-term efficacy than that of KG in treating recurrent oral ulceration (YDFET).
Chronic Disease ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Pain Measurement ; Phytotherapy ; Treatment Outcome ; Yin Deficiency ; drug therapy
3.Phenylethanoid glycosides distribution in medicinal plants of Gesneriaceae.
Zhen-Fang BAI ; Xiao-Qin WANG ; Pei-Gen XIAO ; Yong LIU
China Journal of Chinese Materia Medica 2013;38(24):4267-4270
To investigate the role of distribution and phylogeny of phenylethanoid glycoside in medicinal plants of Gesneriaceae, five phenylpropanoid glycosides, acteoside, paraboside B, isonuomioside A, paraboside II, and paraboside III were quantitatively determined in 12 species of Gesneriaceae by HPLC. The existence and content of these compounds were analyzed. The results showed that phenylethanoid glycosides were found in the most of those plants, but the kind of phenylethanoid glycosides varied in different species. Acteoside distribute in most of this plant group, paraboside B, isonuomioside A, paraboside II, and paraboside III were rare in those plants. The results of this study support morphological viewpoint that Trib. Trichosporeae is more developmental than Trib. Didymocarpeae.
Glucosides
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chemistry
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metabolism
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Magnoliopsida
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metabolism
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Phenylethyl Alcohol
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chemistry
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Plants, Medicinal
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metabolism
4.Effects of angiotensin II on Ca2+ signal in cultured rat cardiac myocytes revealed by confocal laser scanning microscopy.
Xiao-li LIU ; Pei-yong WANG ; Jian LIU
Chinese Journal of Applied Physiology 2005;21(2):163-165
AIMThe effects of angiotensin II on the changes of Ca2+ signal in cultured rat neonatal myocytes were investigated in order to reveal the localization and distribution of elementary Ca2+ signaling units.
METHODSThe cultured neonate rat myocytes were treated with angiotensin II, and calcium signal was detected using confocal laser scanning microscopy and fluo-4/AM calcium probe.
RESULTSThe propagation of Ca2+ waves was observed in rest and angiotensin II stimulated cardiac myocytes. Calcium fluorescent intensity oscillated slightly in myocytes and the average intensity was much higher in the nucleus than in the cytosol, all of which could be magnified significantly by AngII (10(-6) mol/L). Ca2+ oscillation induced by Ang II was completely blocked by NO donor sodium nitroprusside. AngII evoked Ca2+ sparks close to the cell surface membrane, and couldn't be abolished by sodium nitroprusside.
CONCLUSIONThere are spatiotemporal dynamics of Ca2+ signaling patterns such as Ca2+ wave, Ca2+ spikes, Ca2+ oscillation and the whole cell Ca2+ transients induced by angiotensin II, which might play very important roles in cellular cardiac function.
Angiotensin II ; pharmacology ; Animals ; Calcium ; metabolism ; Calcium Signaling ; Cells, Cultured ; Microscopy, Confocal ; Myocytes, Cardiac ; drug effects ; metabolism ; Rats
5.Expression of cell cycle regulator ATM, Chk2 and p53 and their clinicopathological correlation in breast infiltrative ductal carcinoma.
Xiao-juan PEI ; Qing-xu YANG ; Shao-jie LIU ; Min SU ; Zhuo-ya HUANG ; An-jia HAN
Chinese Journal of Pathology 2012;41(7):479-480
Ataxia Telangiectasia Mutated Proteins
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Breast
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metabolism
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pathology
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Breast Neoplasms
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metabolism
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pathology
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Carcinoma, Ductal, Breast
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metabolism
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pathology
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Carcinoma, Intraductal, Noninfiltrating
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metabolism
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pathology
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Cell Cycle Proteins
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metabolism
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Checkpoint Kinase 2
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DNA-Binding Proteins
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metabolism
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Female
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Humans
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Lymphatic Metastasis
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Neoplasm Grading
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Protein-Serine-Threonine Kinases
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metabolism
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Tumor Burden
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Tumor Suppressor Protein p53
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metabolism
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Tumor Suppressor Proteins
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metabolism
6.Aggressive systemic mastocytosis: report of a case.
Mi WANG ; Qun-Pei YANG ; Xiao-Qing WANG ; Xia XU ; Wei-Ping LIU
Chinese Journal of Pathology 2010;39(11):775-777
Antineoplastic Agents
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therapeutic use
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Diagnosis, Differential
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Female
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Histiocytosis, Langerhans-Cell
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metabolism
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pathology
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Humans
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Interferons
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therapeutic use
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Leukemia, Mast-Cell
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metabolism
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pathology
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Mastocytosis, Cutaneous
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metabolism
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pathology
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Mastocytosis, Systemic
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diagnostic imaging
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drug therapy
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metabolism
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pathology
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Middle Aged
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Proto-Oncogene Proteins c-kit
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metabolism
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Radiography
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Radionuclide Imaging
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Spleen
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pathology
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surgery
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Splenectomy
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Tryptases
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metabolism
7.Review of research and development and significant effect of Hippophae rhamnoides.
Yong LIU ; Yong-Shan LIAN ; Ying-Li WANG ; Min-Hui LI ; Pei-Gen XIAO
China Journal of Chinese Materia Medica 2014;39(9):1547-1552
Hippophae rhamnoides is one of the most representative economy crops for its wide uses of biological diversity and abundance of resource. As the key healthy food development and ecology protection, H. rhamnoides has been developed widely. Meanwhile, the development of H. rhamnoides has obtained great achievements. Nowadays, H. rhamnoides is still a necessary economy crop, while it has great influence on ecology protection. This paper discussed the phytochemistry, pharmacology, clinical application and product development, and propounded some suggestions for future research and economy development to get comprehensive benefit of H. rhamnoides and to serve for well-off society.
Biomedical Research
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methods
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trends
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Flavonoids
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chemistry
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therapeutic use
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Hippophae
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chemistry
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Humans
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Molecular Structure
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Phytotherapy
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methods
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trends
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Polyphenols
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chemistry
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therapeutic use
8.Essentials of pharmacophylogeny: knowledge pedigree, epistemology and paradigm shift.
Da-cheng HAO ; Pei-gen XIAO ; Li-wei LIU ; Yong PENG ; Chun-nian HE
China Journal of Chinese Materia Medica 2015;40(17):3335-3342
Chinese materia medica resource (CMM resource) is the foundation of the development of traditional Chinese medicine. In the study of sustainable utilization of CMM resource, adopting innovative theory and method to find new CMM resource is one of hotspots and always highlighted. Pharmacophylogeny interrogates the phylogenetic relationship of medicinal organisms (especially medicinal plants), as well as the intrinsic correlation of morphological taxonomy, molecular phylogeny, chemical constituents, and therapeutic efficacy (ethnopharmacology and pharmacological activity). This new discipline may have the power to change the way we utilize medicinal plant resources and develop plant-based drugs. Phylogenomics is the crossing of evolutionary biology and genomics, in which genome data are utilized for evolutionary reconstructions. Phylogenomics can be integrated into the flow chart of drug discovery and development, and extends the field of pharmacophylogeny at the omic level, thus the concept of pharmacophylogenomics could be redefined in the context of plant pharmaceutical resources. This contribution gives a brief discourse of knowledge pedigree of pharmacophylogeny, epistemology and paradigm shift, highlighting the theoretical and practical values of pharmacophylogenomics. Many medicinally important tribes and genera, such as Clematis, Pulsatilla, Anemone, Cimicifugeae, Nigella, Delphinieae, Adonideae, Aquilegia, Thalictrum, and Coptis, belong to Ranunculaceae family. Compared to other plant families, Ranunculaceae has the most species that are recorded in China Pharmacopoeia (CP) 2010. However, many Ranunculaceae species, e. g., those that are closely related to CP species, as well as those endemic to China, have not been investigated in depth, and their phylogenetic relationship and potential in medicinal use remain elusive. As such, it is proposed to select Ranunculaceae to exemplify the utility of pharmacophylogenomics and to elaborate the new concept empirically. It is argued that phylogenetic and evolutionary relationship of medicinally important tribes and genera within Ranunculaceae could be elucidated at the genomic, transcriptomic, and metabolomic levels, from which the intrinsic correlation between medicinal plant genotype and metabolic phenotype, and between genetic diversity and chemodivesity of closely related taxa, could be revealed. This proof-of-concept study regards pharmacophylogenomics as the updated version of pharmacophylogeny and would enrich the intension and spread the extension of pharmacophylogeny. The interdisciplinary knowledge and techniques will be integrated in the proposed study to promote development of CMM resource discipline and to boost sustainable development of Chinese medicinal plant resources.
China
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Knowledge
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Medicine, Chinese Traditional
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Phylogeny
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Plants, Medicinal
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chemistry
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classification
;
genetics
9.Effect of recombinant human edostatin on peritoneal angiogenesis in uremic peritoneal dialysis rats
Zhanzheng ZHAO ; Ying CAO ; Zhangsuo LIU ; Jing XIAO ; Pei WANG ; Xianhui LIANG
Chinese Journal of Nephrology 2010;26(10):791-795
Objective To study the effect of recombinant human edostatin on peritoneal angiogenesis in uremic peritoneal dialysis(PD) rats. Methods Forty male SD rats were randomly divided into 5 groups: normal control rats (group 1), renal failure without PD rats (group 2), rats dialyzed with 4.25% PD solution (group 3), rats dialyzed with 4.25% PD solution and received subcutaneous injection of recombinant human endostatin 10 mg/kg (group 4), rats dialyzed with 4.25% PD solution and received subcutaneous injection of recombinant human endostatin 40 mg/kg (group 5). Recombinant human endostatin was given every other day during peritoneal dialysis period, total 14 times. After regular PD for 28 days, tissue immunohistochemical staining and RT-PCR were used to detect the mRNA and protein expressions of VEGF and bFGF in peritoneal tissues of each group rats. Microvessel density (MVD) of peritoneum was detected and quantified with anti-CD34 immunohistochemical staining. Results The mRNA and protein of VEGF and bFGF were expressed in each group. Compared to group 1, the mRNA and protein expression of VEGF and bFGF were significantly up-regulated in group 2 and group 3 (all P<0.05). Compared with group 3, the mRNA and protein expression of VEGF and bFGF were significantly downregulated in group 4 and group 5 (all P<0.05). Compared with group 4, the mRNA and protein expression of VEGF and bFGF were significantly down-regulated in group 5 (all P<0.05). The new microvascular vessels in group 1 showed little or none. Compared with group 1, MVD was significantly increased in group 2 and group 3 (P<0.05). Compared with group 3, MVD was significantly decreased in group 4 and group 5 (all P<0.05). Conclusions Recombinant human endostatin can effectively inhibit rat peritoneal neoangiogensis. Down-regulated expression of VEGF and bFGF in peritoneum may be one of the mechanisms of recombinant human endostatin inhibiting peritoneal angiogenesis.
10.Screening and identification of neutralizated single-chain antibody of anti-glomerular basement membrane antibody
Jing XIAO ; Zhangsuo LIU ; Pei WANG ; Liuyu HUANG ; Hongbin SONG ; Minghui ZHAO
Chinese Journal of Microbiology and Immunology 2010;30(9):791-794
Objective To screen a human single-chain variable fragments(scFv)against antiGBM antibody.Methods Using phage display technique,the phage antibody library was panned by antiglomerular basement membrane(GBM)antibody which was coated in a micro-titer plate,one clone was found to have high affinity to anti-GBM antibody.The DNA sequence of the positive clone was determined.Results Along with the increase of rounds anti-GBM antibody specific phage antibody was highly enriched and screening efficiency was increased 137 folds than the firest round.ELISA and competition inhibition assay showed that the scFv had a specific combination character with anti-GBM antibody.DNA sequencing confirmed that the whole gene of scFv was 750 bp,and in accordance with humanized single-chain variable region antibody sequence structure.Conclusion The results suggested that the scFv fragment to anti-GBM antibody could be successfully selected by recombinant phage antibody technique,which will laid an experimental foundation for further research of the therapy of Goodpasture syndrome.