Objective To preliminary investigate the cytoactive of HELA cell after PUMA gene promoting HELA cell apoptosis and whether or not the protein of cyt-c,AIF in the chondrosome have participated in the process of PUMA gene inducing HELA cell.Methods we Used AO/EB dyeing to detect the Morphologic change of cell induces by PUMA gene and Western Blot to detect whether or not the position of cyt-c,AIF in the chondrosome have transferred after PUMA gene induces HELA cell apoptosis.Result ①Fluorescence microscope and inverted phase contrast microscope display HELA cell have a series of apoptosis Morphologic change after transfecting PUMA into HELA cell,and this Morphologic change of cell is more obviously after transfecting 48h than after 24h.;②Western Blot display the protein cyt-c and AIF transferred toward to kytoplasm after HELE cell took place apoptosis,the mount of transfecting protein is more obviously after transfecting 48h than after 24h.Conclusion ① PUMA gene have the effect of promoting HELA cell apoptosis;②cyt-c and AIF take part in the process of promoting HELA cell apoptosis,but during this process if the membrane potential of chondrosome was changing need to do some more study to confirm it.

Objective: To explore the value of cadaveric vein allografts in hepatic vein reconstruction in adult-to-adult living donor liver transplantation (LDLT). Methods: The clinical data of 9 recipients, who underwent LDLT (including the hepatic vein reconstruction) in the right liver lobe without middle hepatic vein (MHV) from June 2007 to January 2008, were retrospectively analyzed. The cadaveric vein allografts were stored in 4°C UW solution within seven days and were used for construction of large hepatic veins such as: tributaries of the middle hepatic vein from V, VIII and right inferior hepatic vein. Results: Cadaveric interposition vein allografts were used for venous reconstruction in 9 (81.8%) of 11 cases receiving a modified right liver graft, 6 cases receiving one-vein reconstruction, cases receiving two-vein reconstruction and 1 case receiving three-vein reconstruction. Only 1 recipient died of renal failure and severe pulmonary infection on day 14 after transplantation without any hemiliver venous outflow obstruction. Doppler ultrasound showed no thrombosis and the blood flowed smoothly in the other 7 recipients during the 9 to 15 months follow-up period. The cumulative patency rates of the 8 survivals for interposition vein grafts were 72.7% (8/11), 54.5% (6/11), and 36.5% (4/11) at 3, 6, and 9 months, respectively. The regeneration of paramedian sectors was equivalent. Conclusion: Cadaveric vein allograft is safe, simple, and effective in adult-to-adult right lobe living donor liver transplantation for hepatic vein reconstruction.

Objective: To investigate the modification of the technique for establishing rat model of orthotopic liver transplantation. Methods: Male Sprague-Dawley rats with similar body weights were selected as the donors and recipients. Two protocols were used for establishing orthotopic liver transplantation model in rats: one was Kamada's two-cuff method and the other was modified two-cuff method. The major modifications included the improvement of the anastomosis of superior and inferior vena cava, improvement of anastomosis between the portal vein and hepatic inferior vena cava, and the improvement of total bile duct reconstruction. The successful rate of operation, total operation time, anhepatic time, and the recipient operative time were recorded and compared in 2 groups. Results: The successful rates of the original and modified methods were 78.9%(71/90) and 91.7% (55/60), respectively(P<0.05). No differences were found between the 2 groups in the total operative time, anhepatic time, and the recipient operative time. Conclusion: The modified model is simple and easy to be made, and can serve as a stable model for the study of liver transplantation.

BACKGROUND:DCLK1 is a transmembrane microtubule-associated kinase in neurons after mitotic division, which may be the intestinal cancer stem cel marker. OBJECTIVE:To observe the expression and pathological significance of DCLK1 and Ki67 in colorectal cancer. METHODS: Expression of Ki67 and DCLK1 in 150 cases of colorectal cancer tissues was detected by immunohistochemical method in contrast to normal colorectal mucosa, para-carcinoma tissue, and adenoma tissue. RESULTS AND CONCLUSION:The expression rates of DCLK1 and Ki67 were 36.7% and 34.7% in cancer tissues, respectively, both of which were significantly higher than those in normal colorectal mucosa and adenoma. The expression of DCLK1 was associated with the location, depth of invasion, lymph node metastasis (P < 0.05), while the expression of Ki67 was just associated with the depth of invasion (P < 0.05). There was a negative correlation between the expression of DCLK1 and Ki67 (r=-0.460,P=0.000). The count of DCLK1+/Ki67-cels was about 2.01% in colorectal cancer tissues, and these cels mainly distributed at the bottom of intestinal mucosa base and common duct wal. DCLK1+/Ki67- cels were oval, the nuclei were large and deep-stained with prominent nucleolus, and there was rare nuclear fission and less cytoplasm. From the aspects of cel number, location, and cel morphology, DCLK1+/Ki67- cels are in line with the characteristics of cancer stem cels; therefore, DCLK1+/Ki67-can be used as a cancer stem cel marker of colorectal cancer.

Acupuncture Therapy ; Child ; Child, Preschool ; Female ; Humans ; Male ; Narcolepsy ; therapy ; Qi

Objective:To explore the methods of emergency terminating refractory tachyarrhythmia.Methods:Data of 117 cases,Who received emergency termination of tachyarrhythmia in emergency department of Taihe Hospital in the last five years,Were summarized and revieWed.Results:All 117 cases With tachyarrhythmia Were successfully terminated in emergency.A total of 21 cases (17.9%)Were successfully terminated in emergency using tWo or more methods (12 cases With supraventricular tachycardia,five cases With ventricular tachycardia,one case With atrial flutter and three cases With atrial fibrillation),in these patients concluded as refractory tachyarrhythmia,five pa-tients (23.8%)Were terminated via synchronous electrical cardioversion.Conclusion:Termination of refractory tachyarrhythmia needs individualization treatment strategy,its treatment may be drugs first and then electrical car-dioversion,or electrical cardioversion directly if necessary.

Anticoagulants ; therapeutic use ; Child ; Coagulants ; blood ; Heparin, Low-Molecular-Weight ; therapeutic use ; Humans ; Purpura, Schoenlein-Henoch ; blood ; drug therapy ; Signal Transduction ; Thromboplastin

Objective To investigate the relapse risk assessment for patients with acute promyelocytic leukemia(APL)through testing PML-RAR? fusion gene by real-time quantitative polymerase chain reaction(RQ-PCR).Methods Relative copies of PML-RAR? fusion gene were measured in 25 patients with APL in phases of first diagnosis,complete remission(CR)and relapse.The minimal residual disease(MRD)situations were also monitored in 6 of the 25 patients.Results Different PML-RAR? fusion gene expression levels were observed in patient groups of different phases of the disease.(P

OBJECTIVE To observe the protective effect and underlying mechanism of total ginsenosides (TG) on bleomycin-induced pulmonary fibrosis. METHODS Intratracheal instillation of bleomycin 5 mg · kg-1 was conducted to establish a pulmonary fibrosis mouse model. Kunming mice(1/2 males and 1/2 females)were randomly divided into sham-operation(Sham),model,total ginsen?osides 40,80 and 160 mg·kg-1 and prednisone acetate(5 mg·kg-1) groups. After 28 d administration,the histopathological changes in the lung were analyzed by hematoxylin eosin(HE)and Masson staining. The exprssion of alpha smooth muscle actin(α-SMA)in the lung was detected by real-time PCR. The content of hydroxyproline(HYP)and glutathione(GSH),level of total antioxidant capacity(T-AOC)and hydroxy radical(·OH),activity of myeloperoxidase(MPO)and nitric oxide synthase(NOS)in the lung were detected by corresponding kits. RESULTS Compared with the sham group,the pulmonary indexes in model group were significantly increased(P<0.01),alveolitis and pulmonary fibrosis were obvious. The mRNA expression ofα-SMA,content of HYP and · OH,activity of MPO and NOS were increased(P<0.05),but the content of GSH and T-AOC in model group was decreased(P<0.05). Compared with model group,the pulmonary indexes in TG 80 and 160 mg · kg-1 and prednisone acetate 5 mg · kg-1 groups were reduced(P<0.05),and the degree of alveolitis and pulmonary fibrosis was mitigated. The mRNA expression ofα-SMA,content of HYP and · OH, the activity of MPO and NO were decreased (P<0.05),while the content of GSH and T-AOC was increased(P<0.05). CONCLUSION TG can improve the degree of mice pulmonary fibrosis induced by bleomycin. The mechanism may be related to the increased antioxidant capacity of organisms.

The application of structured education in patients with type 2 diabetes mellitus showed significant effect. This paper introduced relevant content of structured education, the application status quo of structured education in patients with type 2 diabetes mellitus at home and abroad, aiming to provide reference for exploring health education models of patients with diabetes mellitus and reduce patients' complications, thus improving their quality of life.