1.Laser-induced choroidal neovascularization inhibited by tetrandrine in rats
Dai LI ; Shuiqing ZENG ; Xiao CHEN
Chinese Journal of Ocular Fundus Diseases 2000;0(04):-
Objective To investigate the effect of tetrandrine (Tet) on experimental choroidal neovascularization and the effect of Tet on retinal structure and function. Methods Choroidal neovascularization was induced in 20 Brown Norway (BN) rats (40 eyes) by diode laser (wavelength: 810 nm; exposal time: 0.1 second; facular diameter:100 ?m; energy: 120 mW), and the rats were divided randomly into experimental and control group with 10 rats (20 eyes) in each group. In experimental group, 0.05 ml Tet with the concentration of 3.21 ?mol/L was injected intravitreously 0 and 3 days after laser photocoagulation; in the control group, the rats underwent an intravitreous injection with the same volume of sodium chloride solution. The incidence of CNV was evaluated by fundus fluorescein angiography (FFA) 14 days after laser photocoagulation. Five right eyes of another Five healthy BN rats underwent intravitreous injection with 0.05 ml Tet with the concentration of 3.21 ?mol/L, and an intravitreous injection with the same volume of sodium chloride solution was performed on the left eyes. Before injection, 1 hour, and 1 day after the first injection, and 1 hour, 1 day, 7 days, 14 days after the second injection the electroretinography (ERG) was performed on these 5 rats; 14 days after the second injection, the retinae were examined by light microscopy and transmission electron microscopy. Results The incidence of CNV was 23.26% in experimental group,which was obviously lower than that in the control group (63.33%) (P0.05). There were no structural changes of retinal tissues examined by light and electron microscopy. Conclusion Tet may inhibit choroidal neovascularization in rats; there isn′t any significant toxic effect of intravitreous injection with Tet on retina at the dosage of 3.21 ?mol/L.
2.Comment on pathology research of malignant lymphoma.
Chinese Journal of Pathology 2006;35(4):193-196
Antiviral Agents
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therapeutic use
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Common Variable Immunodeficiency
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complications
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Epstein-Barr Virus Infections
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complications
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Humans
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Interferon-alpha
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therapeutic use
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Lymphoma
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etiology
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pathology
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therapy
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Pathology, Clinical
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methods
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trends
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Prognosis
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Zidovudine
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therapeutic use
3.Updates on lymphoma pathology.
Chinese Journal of Pathology 2009;38(11):721-723
Gene Expression Profiling
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Gene Rearrangement
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Humans
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Lymphoma
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classification
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genetics
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pathology
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Lymphoma, Follicular
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classification
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genetics
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pathology
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Lymphoma, Large B-Cell, Diffuse
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classification
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genetics
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Lymphoma, T-Cell
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classification
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genetics
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Translocation, Genetic
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World Health Organization
4.Current research status of serum tumor markers of pancreatic cancer
Linshan ZENG ; Weidong XIAO ; Yong LI
International Journal of Surgery 2010;37(12):839-843
The incidence of pancreatic cancer is gradually increasing worldwide. The overall 5-year survival rate of pancreatic cancer is about 5%, because the early diagnosis is difficult and the radical resection rate is low in pancreatic cancer. The keys of improving the poor prognosis of pancreatic cancer are early diagnosis and radical resection. Detection of serum tumor markers has an important utility in the diagnosis, prognosis and surveillance of pancreatic cancer. CA19-9 is the most widely used and best validated serum tumor marker for pancreatic cancer although it has some limits. With the development of molecular biological techniques in recent years, several potential serum tumor markers for pancreatic cancer are undergoing evaltation, including MIC- 1, M2- PK, OPN, RCAS1, and so on. This paper is to review the current status of serum tumor markers of pancreatic cancer.
6.Therapy for brain metastasis from non-small cell lung cancer
Yin-duo ZENG ; Xiao-xiao DINGLIN ; Li-kun CHEN ;
Journal of International Oncology 2011;38(7):537-540
Therapeutic approaches to brain metastases from non-small cell lung cancer ( NSCLC ) include corticosteroids, anticonvulsants, surgery, radiotherapy and chemotherapy. In recent years, molecular targeted therapy such as the epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) has become a new option. This article discussed the roles of surgery, brain radiation, chemotherapy, targeted therapy , and other new directions in the treatment of patients with brain metastases from NSCLC.
7.Effects of turicamycin on proliferation of high glucose-induced glomerular mesangial cells and its mechanism study
Songmin HUANG ; Wenli CAI ; Li XIAO ; Li ZENG ; Li ZHOU
Chinese Journal of Nephrology 1997;0(01):-
Objective To observe the effects of turicamycin-inhibitor of N-glycosylation of proteins on expression of (?1 integrin,FAK and cyclin D1 in high glucose-induced glomerular mesangial cells (CMC), and to explore the mechanism concerned. Methods Cultured HBZY-1 rat mesangial cells were divided into 5 groups:control group;high glucose group;mannitol group;high glucose plus TM group; TM group.The expression of ?1 integrin was measured by flow cytometry, expression of FAK and cyclin D1 was measured by immunohistochemistry,and proliferation of GMCs was measured by MTT. Results There was a little expression of ?1 integrin, FAK and cyclin D1 on normal mesangial cells. High glucose induced the proliferation and increased the expression of ?1 integrin and cyclin D1.There was no significant difference in mannitol group as compared to control group.The expression of (?1 integrin FAK and cyclin D1 decreased notably by TM.TM could also decrease proliferative abilitiy of cells. All the effects of TM were dose-dependent. Conclusion Through blocking glycosylation of glycoprotein, TM can suppress cellular proliferation and expression of pi integrin induced by high glucose in a dose-dependent manners,then pi integrin affects the expression of FAK and cyclin D1.
8.An experimental study on effects of local vibration on the serum concentration of tumor necrosis factor in rabbits.
Li LIN ; Chun-zhi ZNANG ; Qiang ZHANG ; Xiao-li ZENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(2):142-143
Animals
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Female
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Male
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Rabbits
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Tumor Necrosis Factor-alpha
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analysis
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Vibration
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adverse effects
9.Research of the mechanism of pre-B-cell colony-enhancing factor in pulmonary vascular endothelial permeability increasing after CPB
Yuan ZENG ; Wei YANG ; Xiaoqiang ZHANG ; Bin LI ; Xiao DONG
Chongqing Medicine 2013;(28):3388-3389,3392
Objective To investigate the mechanism of PBEF in pulmonary vascular endothelial permeability increasing after car-diopulmonory bypass ,in order to provide the basis for a better lung protective measures during cardiopulmonary bypass .Methods Animal models were established ,group A :the rats were transfected with the lentiviral AD-PBEFshRNA ;group B :the rats were took 30 min deep hypothermic circulatory arrest ;group C :the rats were took 30 min deep hypothermic circulatory arrest ,then trans-fected with the lentiviral AD-PBEFshRNA ;control group :the rats were anesthetized and established CPB tube ,without CPB by-pass .Lung tissue was detected with Western blotting and ELISA .Results PBEF ,phosphorylation of P38MAPK ,ERK ,MLC ,VE-cadherin ,FAK in group C had significant difference with group A ,B and the control group(P<0 .05) .VEGF ,MMP2 ,MMP9 ,W/D in group C had significant difference with group A ,B and the control group(P<0 .05) .The pathological results showed that rat lung tissue of the control group was normal ,while in group C ,it had severe pathological damage ,the pathological damage degree in group A ,B reduced compared to group C .Conclusion PBEF can through MAPK/PI3K-Akt/VEGF signaling pathway ,increase pulmonary vascular endothelial permeability .
10.The therapeutic effects of 3′-daidzein sulfonate sodium on isolated myocardial ischemia/reperfusion injury and its relation with anti-oxidation
Zhihua HUANG ; Liangdong LI ; Hai XIAO ; Xianhua HUANG ; Jing ZENG
Chinese Pharmacological Bulletin 1986;0(04):-
Aim To explore the therapeutic effects of 3′-daidzein sulfonate sodium(DSS) on isolated myocardial ischemia/reperfusion injury and the relation of its mechanism to anti-oxidation.Methods An isolated rat myocardial ischemia/reperfusion injury was made,and the injury was treated with DSS.Following the treatment,the coronary blood flow levels,left ventricular systolic pressure,and activities of enzymes(LDH,SOD,GSH-Px) were measured.Results After treatment,the isolated myocardial tissue showed an increase in the volume of perfusion and an increase in the enzymatic activities of LDH,SOD,GSH-Px in the myocardial tissue.Conclusion DSS has a therapeutic effect on isolated myocardial ischemia/reperfusion injury because it enhances anti-oxidative activity in myocardial tissue.